Abstract：
:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34+ cells, colony-forming unit-granulocyte-macrophage (CFU-GM), and total CFU were analyzed. Twenty UCB units cryopreserved using the automated system and stored for a median of 34 days were analyzed. Mean CD34+ cell viabilities before freezing were 99.8+/-0.5% and after thawing were 99.8+/-0.4% in the large bag compartments and 99.7+/-0.5% in the small compartments. The mean recovery values for total nucleated cells, CD34+ cells, CFU-GM, and total CFU were 94.8+/-16.0%, 99.3+/-18.6%, 103.9+/-20.6%, and 94.3+/-12.5%, respectively in the large compartments, and 95.8+/-25.9%, 106.8+/-23.9%, 101.3+/-23.3%, and 93.8+/-19.2%, respectively in the small compartments. A small-scale automated cryopreservation and storage system did not impair the clonogenic capacity of UCB HPCs. This cryopreservation system could provide cellular products adequate for UCB banking and HPC transplantation.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Miura J,Minegishi M,Itoh T,Kitaura T,Fukawa N,Takahashi H,Suzuki A,Kudo Y,Narita A,Sato Y,Suzuki M,Wada Y,Takeyama Y,Watanabe T,Tsuchiya Sdoi
10.1016/j.cryobiol.2008.07.004subject
Has Abstractpub_date
2008-10-01 00:00:00pages
178-81issue
2eissn
0011-2240issn
1090-2392pii
S0011-2240(08)00087-4journal_volume
57pub_type
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