Effect of cryoprotectants on the viability and function of unfrozen human polymorphonuclear cells.

abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...

journal_title：Cryobiology

authors： Liu Y,Xu T,Robinson N,Qin J,Li X

更新日期：2014-04-01 00:00:00

abstract：:This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze-thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statisticall...

journal_title：Cryobiology

authors： Lyu SR,Wu WT,Hou CC,Hsieh WH

更新日期：2010-04-01 00:00:00

abstract：:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...

journal_title：Cryobiology

authors： Hashimoto M,Schmid HA,Ohwatari N,Pleschka K

更新日期：1998-11-01 00:00:00

abstract：:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (B...

journal_title：Cryobiology

authors： Browne RK,Clulow J,Mahony M,Clark A

更新日期：1998-12-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...

journal_title：Cryobiology

authors： Niknejad H,Deihim T,Solati-Hashjin M,Peirovi H

更新日期：2011-12-01 00:00:00

abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...

journal_title：Cryobiology

authors： Mu F,Niu L,Li H,Liao M,Li L,Liu C,Chen J,Li J,Zuo J,Xu K

更新日期：2013-02-01 00:00:00

abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...

journal_title：Cryobiology

authors： Soule E,Bandyk M,Matteo J

更新日期：2018-06-01 00:00:00

abstract：:The process of ice formation and propagation during cryopreservation impacts on the post-thaw outcome for a sample. Two processes, either network solidification or progressive solidification, can dominate the water-ice phase transition with network solidification typically present in small sample cryo-straws or cryo-v...

journal_title：Cryobiology

authors： Kilbride P,Morris GJ,Milne S,Fuller B,Skepper J,Selden C

更新日期：2014-12-01 00:00:00

abstract：:Temperature may have significant influence on vascular tone in such cases as organ preservation, coronary bypass surgery, and extracorporeal circulation. The aim of this research was to study the direct effect of temperature variation on vascular tone in an attempt to elucidate the mechanisms involved. In a first seri...

journal_title：Cryobiology

authors： Herrera B,Eisenberg G,Holberndt O,Desco MM,Rábano A,García-Barreno P,Del Cañizo JF

更新日期：2000-08-01 00:00:00

abstract：:The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...

journal_title：Cryobiology

authors： Arnaud FG,Khirabadi B,Fahy GM

更新日期：2003-06-01 00:00:00

abstract：PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

journal_title：Cryobiology

authors： Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

更新日期：2017-04-01 00:00:00

abstract：:Boar spermatozoa are extremely sensitive to low temperatures and the cryopreservation causes dramatic changes in sperm survivability, but it is not clear which part of the cryopreservation process affects the most. The aim of this work was to assess early events of apoptotic changes as damage indicators in boar sperm ...

journal_title：Cryobiology

authors： Malcervelli DM,Torres P,Suhevic JF,Cisale H,Fischman ML

更新日期：2020-08-01 00:00:00

abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...

journal_title：Cryobiology

authors： Slotsbo S,Maraldo K,Malmendal A,Nielsen NC,Holmstrup M

更新日期：2008-12-01 00:00:00

abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...

journal_title：Cryobiology

authors： Yildiz C,Mullen B,Jarvi K,McKerlie C,Lo KC

更新日期：2018-10-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarcti...

journal_title：Cryobiology

authors： Hájek J,Barták M,Hazdrová J,Forbelská M

更新日期：2016-12-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00

abstract：:The [K+]/[Na+] ratio of rabbit renal cortical slices was used to examine, at 25 degrees C, the effects on viability of three cryoprotectant agents (CPA) (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) in combination with three vehicle solutions (Krebs-Henseleit (K-H), solution A, and RPS-2). Viability asse...

journal_title：Cryobiology

authors： Clark P,Fahy GM,Karow AM Jr

更新日期：1984-06-01 00:00:00

abstract：:Tambaqui (Colossoma macropomum) is a freshwater fish of great importance to aquaculture in several South American countries. Recent studies have developed a protocol for semen cryopreservation in 0.25 and 0.5 mL straws; however, this technique has limitations for fingerling production at a large scale due to the high ...

journal_title：Cryobiology

authors： Maria AN,Carvalho AC,Araújo RV,Santos JP,Carneiro PC,Azevedo HC

更新日期：2015-04-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00

abstract：:A previous study demonstrated that disaccharides, antioxidants, and caspase inhibitors can be used in freezing solutions to reduce the concentration of Me2SO from the current standard of 10% (v/v) to 5% (v/v) or 2.5% and to eliminate fetal bovine serum (FBS) for the cryopreservation of human amniotic fluid-derived ste...

journal_title：Cryobiology

authors： Cho HJ,Lee SH,Yoo JJ,Shon YH

更新日期：2014-04-01 00:00:00

abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...

journal_title：Cryobiology

authors： Marco-Jiménez F,Garzón DL,Peñaranda DS,Pérez L,Viudes-de-Castro MP,Vicente JS,Jover M,Asturiano JF

更新日期：2006-08-01 00:00:00

abstract：:The Arrhenius plots of inactivation (killing) rates of five mammalian cell lines, V-79 Chinese hamster lung, mouse L-929, mouse neuroblastoma, human 18LU, and human erythrocytes, exposed to hypothermia contain a break somewhere between 5 and 10 degrees C caused by a change from positive to negative slope, which corres...

journal_title：Cryobiology

authors： Kruuv J,Glofcheski DJ,Lepock JR

更新日期：1995-04-01 00:00:00

abstract：:Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...

journal_title：Cryobiology

authors： Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

更新日期：2011-06-01 00:00:00

abstract：:Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...

journal_title：Cryobiology

authors： Paynter SJ,Fuller BJ,Shaw RW

更新日期：1999-09-01 00:00:00

abstract：:The development of drugs in cardiovascular research involves in vitro studies that are generally performed on freshly isolated blood vessels. Naturally, data obtained from human tissues would be the most accurate and predictable for human pharmacology. However, the main problems are that the supply of fresh human mate...

journal_title：Cryobiology

authors： Müller-Schweinitzer E

更新日期：1994-02-01 00:00:00

abstract：:We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...

journal_title：Cryobiology

authors： Katkov II,Kim MS,Bajpai R,Altman YS,Mercola M,Loring JF,Terskikh AV,Snyder EY,Levine F

更新日期：2006-10-01 00:00:00

abstract：:Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

journal_title：Cryobiology

authors： Choi JK,Huang H,He X

更新日期：2015-06-01 00:00:00

abstract：:We investigated cryopreservation of horizontal transmission stages of Holospora undulata, a micronucleus-specific bacterial parasite of Paramecium caudatum. Unlike in previous studies on related Holospora species, protocols using glycerol as cryoprotectant failed entirely. In contrast, freezing with dimethyl sulfoxide...

journal_title：Cryobiology

authors： Millot L,Kaltz O

更新日期：2006-02-01 00:00:00