Physiological evaluation of a rabbit kidney perfused with VS41A.

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00

abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...

journal_title：Cryobiology

authors： Benson JD

更新日期：2012-04-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...

journal_title：Cryobiology

authors： Hara H,Tagiri M,Hwang IS,Takahashi M,Hirabayashi M,Hochi S

更新日期：2014-06-01 00:00:00

abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

journal_title：Cryobiology

authors： Karow AM Jr,Jeske AH

更新日期：1976-08-01 00:00:00

abstract：:Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and ...

journal_title：Cryobiology

authors： Puts CF,Berendsen TA,Bruinsma BG,Ozer S,Luitje M,Usta OB,Yarmush ML,Uygun K

更新日期：2015-08-01 00:00:00

abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...

journal_title：Cryobiology

authors： Izamis ML,Perk S,Calhoun C,Uygun K,Yarmush ML,Berthiaume F

更新日期：2015-10-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

journal_title：Cryobiology

authors： Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

更新日期：1995-08-01 00:00:00

abstract：:The present study aimed to examine the behavior of ram spermatozoa subjected to a vitrification process in free-egg yolk diluents in relation with conventional diluents and cryopreservation protocol used in this species. Previously it was investigated the toxicity of cryoprotectants, sucrose and glycerol, based on dif...

journal_title：Cryobiology

authors： Jiménez-Rabadán P,García-Álvarez O,Vidal A,Maroto-Morales A,Iniesta-Cuerda M,Ramón M,del Olmo E,Fernández-Santos R,Garde JJ,Soler AJ

更新日期：2015-08-01 00:00:00

abstract：:Cryopreservation procedures cause osmotic stress to spermatozoa following cryoinjury and reduce their content of osmolytes. Conventional method for cryoprotectant loading and dilution on canine semen freezing which could be categorized in single step protocol, makes decreasing in sperm performance such as motility, mo...

journal_title：Cryobiology

authors： Setyawan EM,Kim MJ,Oh HJ,Kim GA,Jo YK,Lee SH,Choi YB,Lee BC

更新日期：2015-10-01 00:00:00

abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

journal_title：Cryobiology

authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

更新日期：2010-12-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:Cryoablation has become a popular modality to treat a variety of malignant tumors in solid organs and soft tissues. In the future, the use of cryoablation should focus on its abscopal effect. The present review discusses the increased immune response triggered by cryoablation alone or by cryoablation combined with imm...

journal_title：Cryobiology

authors： Chen J,Qian W,Mu F,Niu L,Du D,Xu K

更新日期：2020-12-01 00:00:00

abstract：:The exchange of genetically engineered mouse strains between research facilities requires transporting fresh mouse sperm under refrigerated temperatures. Although sperm generally maintains fertility for 48 h at cold temperatures, in vitro fertilization rates of C57BL/6 mouse sperm are low after 48-h cold storage. Furt...

journal_title：Cryobiology

authors： Takeo T,Tsutsumi A,Omaru T,Fukumoto K,Haruguchi Y,Kondo T,Nakamuta Y,Takeshita Y,Matsunaga H,Tsuchiyama S,Sakoh K,Nakao S,Yoshimoto H,Shimizu N,Nakagata N

更新日期：2012-12-01 00:00:00

abstract：:In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...

journal_title：Cryobiology

authors： Kusuda S,Teranishi T,Koide N

更新日期：2002-08-01 00:00:00

abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...

journal_title：Cryobiology

authors： Anil S,Rawson D,Zhang T

更新日期：2018-08-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...

journal_title：Cryobiology

authors： Marco-Jiménez F,Garzón DL,Peñaranda DS,Pérez L,Viudes-de-Castro MP,Vicente JS,Jover M,Asturiano JF

更新日期：2006-08-01 00:00:00

abstract：:The Zebrafish has gained increased popularity as an aquatic model species in various research fields, and its widespread use has led to numerous mutant strains and transgenic lines. This creates the need to store these important genetic materials as frozen gametes. Sperm cryopreservation in zebrafish has been shown to...

journal_title：Cryobiology

authors： Bai C,Wang X,Lu G,Wei L,Liu K,Gao H,Huang C,Dong Q

更新日期：2013-10-01 00:00:00

abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

journal_title：Cryobiology

authors： Wang HS,Kang L

更新日期：2005-10-01 00:00:00

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...

journal_title：Cryobiology

authors： Purdy PH,Graham JK

更新日期：2004-02-01 00:00:00

abstract：:Freeze-tolerant third instar larvae of the gallfly Eurosta solidaginis were cooled at 10, 5, 1, and 0.1 degrees C min-1 to -40 degrees C and then warmed to +5 degrees C at 1 degree C min-1. After cooling and warming the larvae were transferred to 21 degrees C and the survival of larvae, success of pupariation, and adu...

journal_title：Cryobiology

authors： Bale JS,Hansen TN,Nishino M,Baust JG

更新日期：1989-06-01 00:00:00

abstract：:There is increasing interest in the possibility of treating diseased or damaged areas of synovial joint surfaces by grafts of healthy allogeneic cartilage. Such grafts could be obtained from cadaver tissue donors or in the future they might be manufactured by 'tissue engineering' methods. Cartilage is an avascular tis...

journal_title：Cryobiology

authors： Pegg DE,Wusteman MC,Wang L

更新日期：2006-06-01 00:00:00

abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....

journal_title：Cryobiology

authors： Zeron Y,Tomczak M,Crowe J,Arav A

更新日期：2002-10-01 00:00:00

abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...

journal_title：Cryobiology

authors： Odintsova NA,Boroda AV,Maiorova MA,Yakovlev KV

更新日期：2017-08-01 00:00:00

abstract：:High concentrations of membrane permeable cryoprotectants are necessary to protect human polymorphonuclear leukocytes from osmotic stress injury during freezing, but there are reports that some cryoprotectants are chemically toxic. Cells were exposed to various concentrations of glycerol, dimethyl sulfoxide, or ethyle...

journal_title：Cryobiology

authors： Takahashi T,Bross JB,Shaber RE,Williams RJ

更新日期：1985-08-01 00:00:00

abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...

journal_title：Cryobiology

authors： Liu Y,Xu T,Robinson N,Qin J,Li X

更新日期：2014-04-01 00:00:00