Abstract：
:Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and unique mechanisms. We hypothesized that polyethylene glycol (PEG) 35 kDa would ameliorate these injury pathways and improve cold primary hepatocyte preservation. We show that reduction of the storage temperature to below zero by means of supercooling, or subzero non-freezing, together with PEG supplementation increases the viable storage time of primary rat hepatocytes in University of Wisconsin (UW) solution from 1 day to 4 days. We find that the addition of 5% PEG 35 kDa to the storage medium prevents cold-induced lipid peroxidation and maintains hepatocyte viability and functionality during storage. These results suggest that PEG supplementation in combination with supercooling may enable a more optimized cell and organ preservation.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Puts CF,Berendsen TA,Bruinsma BG,Ozer S,Luitje M,Usta OB,Yarmush ML,Uygun Kdoi
10.1016/j.cryobiol.2015.04.010subject
Has Abstractpub_date
2015-08-01 00:00:00pages
125-9issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(15)00069-3journal_volume
71pub_type
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