Introduction to the special issue: Thermodynamic aspects of cryobiology.

abstract：OBJECTIVE:Liver metastases occur in approximately 4%-14% of gastric cancer patients and are associated with high mortality. However, no standardized treatment approach is available for these patients. We aimed to assess the clinical outcomes of patients with gastric cancer liver metastases (GCLM) who underwent percutan...

journal_title：Cryobiology

authors： Chang X,Wang Y,Yu HP,Zhang WH,Yang XL,Guo Z

更新日期：2018-06-01 00:00:00

abstract：:Hypothermia is used in the clinic for protection of organs such as the brain against ischemic injury during aortic/complex congenital cardiac surgery or post-resuscitation encephalopathy. The principal mechanism of hypothermic protection is suppression of metabolism, however, the pleiotropic effects of cooling are inc...

journal_title：Cryobiology

authors： Oda T,Shimizu K,Yamaguchi A,Satoh K,Matsumoto K

更新日期：2012-10-01 00:00:00

abstract：:Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...

journal_title：Cryobiology

authors： Hara H,Tagiri M,Hwang IS,Takahashi M,Hirabayashi M,Hochi S

更新日期：2014-06-01 00:00:00

abstract：:The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of th...

journal_title：Cryobiology

authors： Amps KJ,Jones M,Baker D,Moore HD

更新日期：2010-06-01 00:00:00

abstract：:During cryopreservation, spermatozoa are exposed to chemical or physical stress that has adverse effects on the quality of mammalian spermatozoa. Recently, much attention has been paid to environmental contaminants (ECs) in livestock, because of their detrimental effects on livestock productivity and fertility. ECs li...

journal_title：Cryobiology

authors： Naderi N,Hajian M,Souri M,Nasr Esfahani MH,Vash NT

更新日期：2021-01-16 00:00:00

abstract：AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...

journal_title：Cryobiology

authors： Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

更新日期：2017-12-01 00:00:00

abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...

journal_title：Cryobiology

authors： Yildiz C,Mullen B,Jarvi K,McKerlie C,Lo KC

更新日期：2018-10-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...

journal_title：Cryobiology

authors： Hashimoto M,Schmid HA,Ohwatari N,Pleschka K

更新日期：1998-11-01 00:00:00

abstract：:The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection. They were then treated with a...

journal_title：Cryobiology

authors： Teoh KH,Weathers PJ,Cheetham RD,Walcerz DB

更新日期：1996-02-01 00:00:00

abstract：:All the aqueous solutions of linear saturated polyalcohols with four carbons have been investigated at low temperature. Only ice has been observed in the solutions of 1,3-butanediol and 1,2,3- and 1,2,4-butanetriol. For same solute concentration, the glass-forming tendency on cooling is highest with 2,3-butanediol, wh...

journal_title：Cryobiology

authors： Boutron P,Mehl P,Kaufmann A,Angibaud P

更新日期：1986-10-01 00:00:00

abstract：:Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...

journal_title：Cryobiology

authors： Paynter SJ,Fuller BJ,Shaw RW

更新日期：1999-09-01 00:00:00

abstract：:Semen was collected in the laboratory from nine healthy donors. The concentrations and the percentages of live and motile spermatozoa in all semen samples were within the normal range. Each sample was diluted with citrate-egg yolk-glycerol medium with and without 5 mM dithiothreitol (DTT). Samples were frozen in liqui...

journal_title：Cryobiology

authors： Rao B,David G

更新日期：1984-10-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：:The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...

journal_title：Cryobiology

authors： Hunt CJ,Song YC,Bateson EA,Pegg DE

更新日期：1994-10-01 00:00:00

abstract：:The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...

journal_title：Cryobiology

authors： Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

更新日期：2018-02-01 00:00:00

abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...

journal_title：Cryobiology

authors： Solocinski J,Osgood Q,Wang M,Connolly A,Menze MA,Chakraborty N

更新日期：2017-04-01 00:00:00

abstract：:One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study w...

journal_title：Cryobiology

authors： Kanias T,Acker JP

更新日期：2009-04-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

journal_title：Cryobiology

authors： Wang CL,Teo KY,Han B

更新日期：2008-08-01 00:00:00

abstract：:Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyo...

journal_title：Cryobiology

authors： Lavenburg VM,Liao YT,Salvador A,Hsu AL,Harden LA,Wu VCH

更新日期：2020-10-01 00:00:00

abstract：:Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...

journal_title：Cryobiology

authors： O'Neil L,Paynter SJ,Fuller BJ,Shaw RW,DeVries AL

更新日期：1998-08-01 00:00:00

abstract：:The effect of low temperatures on the ultrastructure of the plasma membrane of bull and boar spermatozoa was investigated. Cold-induced changes in the organization of sperm plasma membrane components were demonstrated by the use of fast-freezing combined with freeze-fracture electron microscopy. This preparation techn...

journal_title：Cryobiology

authors： De Leeuw FE,Chen HC,Colenbrander B,Verkleij AJ

更新日期：1990-04-01 00:00:00

abstract：:In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...

journal_title：Cryobiology

authors： Pomorski L,Bartos M,Matejkowska M,Amsolik M,Kuzdak K

更新日期：1999-11-01 00:00:00

abstract：:Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...

journal_title：Cryobiology

authors： Oliver AE,Leprince O,Wolkers WF,Hincha DK,Heyer AG,Crowe JH

更新日期：2001-09-01 00:00:00

abstract：:The aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. The aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in v...

journal_title：Cryobiology

authors： Ahmed H,Jahan S,Khan A,Khan L,Ullah H,Riaz M,Ullah K,Ullah F

更新日期：2020-02-01 00:00:00

abstract：:The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...

journal_title：Cryobiology

authors： Zhurova M,McGann LE,Acker JP

更新日期：2014-06-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00