Non-disaccharide-based mechanisms of protection during drying.

abstract：:The exchange of genetically engineered mouse strains between research facilities requires transporting fresh mouse sperm under refrigerated temperatures. Although sperm generally maintains fertility for 48 h at cold temperatures, in vitro fertilization rates of C57BL/6 mouse sperm are low after 48-h cold storage. Furt...

journal_title：Cryobiology

authors： Takeo T,Tsutsumi A,Omaru T,Fukumoto K,Haruguchi Y,Kondo T,Nakamuta Y,Takeshita Y,Matsunaga H,Tsuchiyama S,Sakoh K,Nakao S,Yoshimoto H,Shimizu N,Nakagata N

更新日期：2012-12-01 00:00:00

abstract：:This study evaluates the effect of control factors on cryopreservation of articular cartilage chondrocytes using the Taguchi method. Freeze-thaw experiments based on the L(8)(2(7)) two-level orthogonal array of the Taguchi method are conducted, and ANOVA (analysis of variables) is adopted to determine the statisticall...

journal_title：Cryobiology

authors： Lyu SR,Wu WT,Hou CC,Hsieh WH

更新日期：2010-04-01 00:00:00

abstract：:In recent years cryotherapy has been more and more frequently used for the treatment of tumors of different organs. Until now, the use of cryotherapy for the treatment of thyroid lesions, as well as histopathologic changes in thyroid tissue after cryotherapy, has not been described. Nitrous oxide cryotherapy of one th...

journal_title：Cryobiology

authors： Pomorski L,Bartos M,Matejkowska M,Amsolik M,Kuzdak K

更新日期：1999-11-01 00:00:00

abstract：:The purpose of our study was to investigate underlying basic mechanisms of hypothermia-induced cardioprotection during oxidative stress in a cardiomyocyte cell culture model. For hypothermic treatment we cooled H9c2 cardiomyocytes to 20°C, maintained 20min at 20°C during which short-term oxidative damage was inflicted...

journal_title：Cryobiology

authors： Diestel A,Drescher C,Miera O,Berger F,Schmitt KR

更新日期：2011-02-01 00:00:00

abstract：:An insect antifreeze protein gene Mpafp698 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis. The gene was constructed and heterogeneously expressed in Escherichia coli as fusion proteins, His-MpAFP698, glutathione S-transferase (GST)-MpAFP698, and maltose-binding protein (MBP)-MpAFP698. ...

journal_title：Cryobiology

authors： Qiu LM,Ma J,Wang J,Zhang FC,Wang Y

更新日期：2010-04-01 00:00:00

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...

journal_title：Cryobiology

authors： Traversari G,Cincotti A

更新日期：2020-11-27 00:00:00

abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...

journal_title：Cryobiology

authors： De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

更新日期：1998-09-01 00:00:00

abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...

journal_title：Cryobiology

authors： Liu Y,Xu T,Robinson N,Qin J,Li X

更新日期：2014-04-01 00:00:00

abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

journal_title：Cryobiology

authors： Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

更新日期：2009-04-01 00:00:00

abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...

journal_title：Cryobiology

authors： Shirazi A,Soleimani M,Karimi M,Nazari H,Ahmadi E,Heidari B

更新日期：2010-04-01 00:00:00

abstract：:Dynamic mechanical analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analy...

journal_title：Cryobiology

authors： Gearing J,Malik KP,Matejtschuk P

更新日期：2010-08-01 00:00:00

abstract：:The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...

journal_title：Cryobiology

authors： Suzuki T,Boediono A,Takagi M,Saha S,Sumantri C

更新日期：1996-10-01 00:00:00

abstract：:Previous reports established that butylated hydroxy toluene (BHT) minimized cold-induced membrane rupture in sperm from several species. No data regarding the specificity of its effect is available. In this study 25 BHT analogs were tested for their effect on bovine sperm membrane stability. Fourteen were membrane lyt...

journal_title：Cryobiology

authors： Graham JK,Hammerstedt RH

更新日期：1992-02-01 00:00:00

abstract：:Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure t...

journal_title：Cryobiology

authors： Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

更新日期：1983-02-01 00:00:00

abstract：:Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...

journal_title：Cryobiology

authors： Jin B,Kleinhans FW,Mazur P

更新日期：2014-06-01 00:00:00

abstract：:Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...

journal_title：Cryobiology

authors： Woods EJ,Liu J,Gilmore JA,Reid TJ,Gao DY,Critser JK

更新日期：1999-05-01 00:00:00

abstract：:As known for different metabolic functions, α-lipoic acid (ALA) has been tested for spermatozoa preservation of animals as well as of human, but not for fish spermatozoa. The present study determined the effects of ALA on short and long-term (cryopreservation) preservation of common carp (Cyprinus carpio) spermatozoa,...

journal_title：Cryobiology

authors： İnanan BE,Kanyılmaz M

更新日期：2020-06-01 00:00:00

abstract：:Advancement in biomedical simulation and imaging modality have catalysed the development of in silico predictive models for cryoablation. However, one of the main challenges in ensuring the accuracy of the model prediction is the use of proper thermal and biophysical properties of the patient. These properties are dif...

journal_title：Cryobiology

authors： Chan JY,Ooi EH

更新日期：2016-12-01 00:00:00

abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...

journal_title：Cryobiology

authors： Goff RP,Quallich SG,Buechler RA,Bischof JC,Iaizzo PA

更新日期：2017-04-01 00:00:00

abstract：:Dipalmitoylphosphatidylcholine (DPPC) bilayers hydrated in the presence of trehalose were equilibrated at various temperatures (4, 20, and 60 degrees C) corresponding to the crystalline Lc, gel L beta', and liquid-crystalline L alpha phases, respectively, and then desiccated at these temperatures or freeze-dried at -8...

journal_title：Cryobiology

authors： Tsvetkova N,Tenchov B,Tsonev L,Tsvetkov T

更新日期：1988-06-01 00:00:00

abstract：:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...

journal_title：Cryobiology

authors： Zhang T,Isayeva A,Adams SL,Rawson DM

更新日期：2005-06-01 00:00:00

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：:The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

journal_title：Cryobiology

authors： Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

更新日期：2001-11-01 00:00:00

abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...

journal_title：Cryobiology

authors： Shaw JM,Trounson AO

更新日期：1989-10-01 00:00:00

abstract：:Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...

journal_title：Cryobiology

authors： Booth KS,James ER,Popiel I

更新日期：1996-06-01 00:00:00

abstract：:Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...

journal_title：Cryobiology

authors： Long JA,Purdy PH,Zuidberg K,Hiemstra SJ,Velleman SG,Woelders H

更新日期：2014-06-01 00:00:00