Osmotic parameters of red blood cells from umbilical cord blood.

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:Despite some 26 published reports addressing oyster sperm cryopreservation, systematic factor optimization is lacking, and sperm cryopreservation has not yet found application in aquaculture on a commercial scale. In this study, the effects of cooling rate, single or combined cryoprotectants at various concentrations,...

journal_title：Cryobiology

authors： Dong Q,Huang C,Eudeline B,Tiersch TR

更新日期：2005-10-01 00:00:00

abstract：:Two parameters fundamental to cell cryobiology are the water permeability (hydraulic conductivity), Lp, and its activation energy, EA. The Lp can be calculated from two experimental determinations: the critical osmolality, Osmcrit, at which 50% of the cells lyse, and the time, tcrit, to 50% lysis in a highly hyposmoti...

journal_title：Cryobiology

authors： Noiles EE,Thompson KA,Storey BT

更新日期：1997-08-01 00:00:00

abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...

journal_title：Cryobiology

authors： Duvivier V,Darquy S,Reach G

更新日期：1999-06-01 00:00:00

abstract：:We have measured the effectiveness of different standard cryogenic liquids (liquid nitrogen, chilled isopentane, chilled hexane, and chilled ethanol) and applied a mathematical model for calculating the temperature changes at different depths in rabbit and rat heads cooled in liquid nitrogen. We have also evaluated th...

journal_title：Cryobiology

authors： Roos MW,Johansson A,Sperber GO

更新日期：1997-09-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...

journal_title：Cryobiology

authors： Hagedorn M,Hsu E,Kleinhans FW,Wildt DE

更新日期：1997-06-01 00:00:00

abstract：:The importance of the frozen phase in the formation of cryooscillations of trypsin activity has been shown in experiments conducted at -10 degrees C under frozen and supercooled conditions, respectively. A solution containing trypsin obtained by trypsinogen activation and 0.1 M MnCl2 was distributed in test tubes with...

journal_title：Cryobiology

authors： Vajda T

更新日期：1992-08-01 00:00:00

abstract：BACKGROUND:The effect of dimethyl sulfoxide (Me2SO) on enumeration of post-thaw CD45+ and CD34+ cells of umbilical cord blood (HPC-C) and mobilized peripheral blood (HPC-A) has not been systematically studied. METHODS:Cells from leukapheresis products from multiple myeloma patients and umbilical cord blood cells were ...

journal_title：Cryobiology

authors： Yang H,Zhao H,Acker JP,Liu JZ,Akabutu J,McGann LE

更新日期：2005-10-01 00:00:00

abstract：:Under optimal freeze-drying conditions, solutions exhibit a cake-like porous structure. However, if the solution temperature is higher than the glass transition temperature of the maximally freeze-concentrated phase (Tg') during drying phase, the glassy matrix undergoes viscous flow, resulting in cake collapse. The pu...

journal_title：Cryobiology

authors： Hara H,Tagiri M,Hwang IS,Takahashi M,Hirabayashi M,Hochi S

更新日期：2014-06-01 00:00:00

abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...

journal_title：Cryobiology

authors： Miura J,Minegishi M,Itoh T,Kitaura T,Fukawa N,Takahashi H,Suzuki A,Kudo Y,Narita A,Sato Y,Suzuki M,Wada Y,Takeyama Y,Watanabe T,Tsuchiya S

更新日期：2008-10-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cry...

journal_title：Cryobiology

authors： Osei-Bempong C,Ghareeb AE,Lako M,Figueiredo FC,Armitage WJ

更新日期：2018-10-01 00:00:00

abstract：:Reperfusion injury has been suggested to cause delayed graft function in renal transplantation. Methods to reduce reperfusion injury could lead to improved clinical renal transplantation. Glycine has been shown to suppress reperfusion injury in rabbit renal tubules and rat hepatocytes. In this study we have determined...

journal_title：Cryobiology

authors： Saunder A,Ametani MS,Belzer FO,Southard JH

更新日期：1993-06-01 00:00:00

abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

journal_title：Cryobiology

authors： Storey KB

更新日期：2004-04-01 00:00:00

abstract：:The efficacy of attenuated strain of gdhA derivative Pasteurella multocida B:2 mutant as a live vaccine to control haemorrhagic septicaemia (HS) disease in cattle and buffaloes has been demonstrated. In order to use P. multocida B:2 mutant as a commercial product, it is essential to optimise its formulation for high v...

journal_title：Cryobiology

authors： Oslan SNH,Halim M,Ramle NA,Saad MZ,Tan JS,Kapri MR,Ariff AB

更新日期：2017-12-01 00:00:00

abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

journal_title：Cryobiology

authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

更新日期：2006-10-01 00:00:00

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00

abstract：:The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...

journal_title：Cryobiology

authors： Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

更新日期：2006-08-01 00:00:00

abstract：:Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...

journal_title：Cryobiology

authors： Purdy PH,Graham JK

更新日期：2004-02-01 00:00:00

abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

journal_title：Cryobiology

authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

更新日期：2008-08-01 00:00:00

abstract：:Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex sper...

journal_title：Cryobiology

authors： Coloma MA,Gómez-Brunet A,Velázquez R,Toledano-Díaz A,López-Sebastián A,Santiago-Moreno J

更新日期：2010-10-01 00:00:00

abstract：:The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...

journal_title：Cryobiology

authors： Worland MR,Block W

更新日期：1999-02-01 00:00:00

abstract：:The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...

journal_title：Cryobiology

authors： Fiser PS,Fairfull RW

更新日期：1986-12-01 00:00:00

abstract：:During cryopreservation, spermatozoa are exposed to chemical or physical stress that has adverse effects on the quality of mammalian spermatozoa. Recently, much attention has been paid to environmental contaminants (ECs) in livestock, because of their detrimental effects on livestock productivity and fertility. ECs li...

journal_title：Cryobiology

authors： Naderi N,Hajian M,Souri M,Nasr Esfahani MH,Vash NT

更新日期：2021-01-16 00:00:00

abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...

journal_title：Cryobiology

authors： Huang KY,de Groot SA,Woelders H,van der Horst GT,Themmen AP,Colenbrander B,van Vlissingen JM

更新日期：2010-04-01 00:00:00

abstract：:Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying ...

journal_title：Cryobiology

authors： Zawlodzka S,Takamatsu H

更新日期：2005-02-01 00:00:00