Abstract：
:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing induces an immunostimulatory signal that creates a T-cell response to the tumor. Six groups of animals (three experimental groups and three control groups) were treated once per week for 4 weeks with different therapies designed to investigate these mechanisms. Three types of immune response were measured: (1) the anti-AT-1 tumor immune titer (Ab response) by serum ELISA, (2) the effect on secondary tumor growth after challenge with live AT-1 cells (size and weight of the secondary tumor over time), and (3) the nature of the immunologic infiltrate into the secondary tumors by immunoperoxidase stain. ELISA showed that immune titers were present in the experimental groups after therapy, but the presence of an immune titer did not have a significant effect on tumor propagation. Histology showed the immunologic infiltrate was similar in all groups. These results showed that an immune response to AT-1 tumor was measurable by serum antibody, but it did not significantly limit secondary tumor growth or affect tumor histology. This suggests that the growth of AT-1 tumors is not inhibited by a cryoimmunological response. Thus, the effect of in vivo cryosurgery in the AT-1 tumor system would likely be limited to cellular and vascular changes.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JCdoi
10.1006/cryo.2001.2305subject
Has Abstractpub_date
2001-02-01 00:00:00pages
59-68issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(01)92305-3journal_volume
42pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.07.001
更新日期：2014-08-01 00:00:00
abstract：:The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(84)90215-3
更新日期：1984-04-01 00:00:00
abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.09.011
更新日期：2009-12-01 00:00:00
abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.01.002
更新日期：2014-04-01 00:00:00
abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.09.001
更新日期：2018-10-01 00:00:00
abstract：:The main aim of the present study was to investigate the effect of sperm freezing medium dilution ratio (1:1, 1:2, and 1:5 v/v), two cryoprotectants: dimethyl sulphoxide (Me(2)SO) and methanol (MeOH), and the addition of foetal bovine serum (FBS) on the cryopreservation of European eel sperm. The effect of these facto...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2006.03.011
更新日期：2006-08-01 00:00:00
abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90066-7
更新日期：1989-10-01 00:00:00
abstract：:Two experiments were conducted to investigate the relationship between survival rates and ultrastructural appearance, using the freeze-replica technique, of bovine blastocysts after vitrification. In experiment 1, blastocysts obtained from in vitro-matured and in vitro-fertilized (IVM-IVF) bovine oocytes were either e...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1994.1051
更新日期：1994-10-01 00:00:00
abstract：:Oxidative stress is one of the major issues associated with cryopreservation because it causes a marked reduction in the post-thaw quality of semen. This study investigated the ability of kinetin to preserve the structural and functional integrity of dog sperm during cryopreservation. Pooled ejaculates were divided in...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.05.015
更新日期：2020-08-01 00:00:00
abstract：:This study analysed the effects of cryopreservation on periodontal regeneration of autotransplanted rat molars. First and second maxillary molars (n=92) of 24 four-week-old Wistar rats were gently extracted and autotransplanted into the abdominal tissue immediately (control group n=44) or after cryopreservation in liq...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.03.008
更新日期：2013-06-01 00:00:00
abstract：:To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.07.003
更新日期：2005-10-01 00:00:00
abstract：:Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insul...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(83)90005-6
更新日期：1983-04-01 00:00:00
abstract：:The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ros...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.10.004
更新日期：2018-12-01 00:00:00
abstract：:The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90030-8
更新日期：1989-02-01 00:00:00
abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.11.015
更新日期：2020-12-04 00:00:00
abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2163
更新日期：1999-06-01 00:00:00
abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2020.04.009
更新日期：2020-06-01 00:00:00
abstract：PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2021.01.005
更新日期：2021-01-02 00:00:00
abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.04.013
更新日期：2018-06-01 00:00:00
abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2182
更新日期：1999-08-01 00:00:00
abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.08.009
更新日期：2009-12-01 00:00:00
abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.2001.2306
更新日期：2001-03-01 00:00:00
abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2106
更新日期：1998-09-01 00:00:00
abstract：:The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(86)90061-1
更新日期：1986-12-01 00:00:00
abstract：:We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(91)90051-o
更新日期：1991-10-01 00:00:00
abstract：:Cryopreservation is a technique that has been extensively used for storage of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine. Therefore, improving current cryopreservation procedures in terms of increasing cell viability and functionality is important. In this study, we optimized the cryopreserv...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.10.145
更新日期：2015-12-01 00:00:00
abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.01.002
更新日期：2017-04-01 00:00:00
abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.05.068
更新日期：2015-08-01 00:00:00
abstract：:Devitrification (ice formation during warming) is one of the primary obstacles to successful organ vitrification (solidification without ice formation). The only feasible approach to overcoming either devitrification or its damaging effects in a large organ appears at present to be the use of some form of electromagne...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(90)90035-3
更新日期：1990-10-01 00:00:00
abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(91)90026-k
更新日期：1991-06-01 00:00:00
