Cryopreservation of small numbers of human spermatozoa in a Stripper tip: report of the first live-birth worldwide.

abstract：:Hypothermia is used in the clinic for protection of organs such as the brain against ischemic injury during aortic/complex congenital cardiac surgery or post-resuscitation encephalopathy. The principal mechanism of hypothermic protection is suppression of metabolism, however, the pleiotropic effects of cooling are inc...

journal_title：Cryobiology

authors： Oda T,Shimizu K,Yamaguchi A,Satoh K,Matsumoto K

更新日期：2012-10-01 00:00:00

abstract：:Cryoablation is a minimally invasive cancer treatment. In this study, the effects of cryoablation on normal rabbit bone were evaluated using imaging and histopathological examinations. Cryoablation was performed using a Cryo-Hit (Galil Medical, Yokneam, Israel). Under anesthesia, one cryoablation needle was inserted a...

journal_title：Cryobiology

authors： Yoshimoto Y,Azuma K,Miya A,Makino E,Nakamoto H,Abe N,Kaburagi M,Ueda H,Kuroda K,Tsuka T,Sugiyama A,Imagawa T,Murahata Y,Itoh N,Osaki T,Shimizu T,Okamoto Y

更新日期：2014-10-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...

journal_title：Cryobiology

authors： Worland MR,Block W

更新日期：1999-02-01 00:00:00

abstract：:In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...

journal_title：Cryobiology

authors： Edashige K,Asano A,An TZ,Kasai M

更新日期：1999-06-01 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...

journal_title：Cryobiology

authors： Moawad AR,Fisher P,Zhu J,Choi I,Polgar Z,Dinnyes A,Campbell KH

更新日期：2012-10-01 00:00:00

abstract：:Cryoablation has become a popular modality to treat a variety of malignant tumors in solid organs and soft tissues. In the future, the use of cryoablation should focus on its abscopal effect. The present review discusses the increased immune response triggered by cryoablation alone or by cryoablation combined with imm...

journal_title：Cryobiology

authors： Chen J,Qian W,Mu F,Niu L,Du D,Xu K

更新日期：2020-12-01 00:00:00

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...

journal_title：Cryobiology

authors： Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

更新日期：2017-12-01 00:00:00

abstract：:Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure t...

journal_title：Cryobiology

authors： Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

更新日期：1983-02-01 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...

journal_title：Cryobiology

authors： Šćekić I,Marinović Z,Lujić J,Müller T,Kitanović N,Urbányi B,Horváth Á

更新日期：2020-08-01 00:00:00

abstract：:The distribution of cryoprotectant (10% glycerol) and ice along the frozen plastic straw (the most useful container for freezing mammalian semen, oocytes and embryos) was studied by Raman scattering technique. Raman spectroscopy being a contactless, non-invasive tool was applied for the straws filled with the cryoprot...

journal_title：Cryobiology

authors： Karpegina YA,Okotrub KA,Brusentsev EY,Amstislavsky SY,Surovtsev NV

更新日期：2016-04-01 00:00:00

abstract：:The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...

journal_title：Cryobiology

authors： Hunt CJ,Song YC,Bateson EA,Pegg DE

更新日期：1994-10-01 00:00:00

abstract：:Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...

journal_title：Cryobiology

authors： Soule E,Bandyk M,Matteo J

更新日期：2018-06-01 00:00:00

abstract：:In cryopreservation procedures, the capacity to protect the cells from freezing and thawing processes is sensitive to the choice of the cryoprotective agent (CPA) and to its optimal concentration. The advancement of research on Tunicate model species has raised interest in liquid nitrogen cryopreservation for the stor...

journal_title：Cryobiology

authors： Sorrenti G,Bagnoli A,Miraglia V,Crocetta F,Vitiello V,Ristoratore F,Cirino P,Sansone G,Sordino P

更新日期：2014-02-01 00:00:00

abstract：:Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...

journal_title：Cryobiology

authors： Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

更新日期：2011-06-01 00:00:00

abstract：:Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...

journal_title：Cryobiology

authors： Anil S,Rawson D,Zhang T

更新日期：2018-08-01 00:00:00

abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

journal_title：Cryobiology

authors： Salmon VM,Leclerc P,Bailey JL

更新日期：2017-02-01 00:00:00

abstract：:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...

journal_title：Cryobiology

authors： Vogiatzi C,Rosenqvist E,Grout BWW

更新日期：2018-05-06 00:00:00

abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

journal_title：Cryobiology

authors： Hao YJ,Liu QL,Deng XX

更新日期：2001-08-01 00:00:00

abstract：:Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex sper...

journal_title：Cryobiology

authors： Coloma MA,Gómez-Brunet A,Velázquez R,Toledano-Díaz A,López-Sebastián A,Santiago-Moreno J

更新日期：2010-10-01 00:00:00

abstract：:The objectives of this study were to identify the presence of different spermatozoa subpopulations (SPs) according to their kinematic characteristics in the sperm of common carp and to test the effects of cryopreservation and prolonged (6-day) storage at room temperature (RT; 23 °C) and 4 °C on spermatozoa motility an...

journal_title：Cryobiology

authors： Marinović Z,Šćekić I,Lujić J,Urbányi B,Horváth Á

更新日期：2021-01-12 00:00:00

abstract：:Cold preservation and reperfusion of liver during transplantation are necessary steps in the procedure but which are also associated with damage to the organ. One aspect of this damage is thought to concern up-regulation of inflammatory markers, such as the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) ...

journal_title：Cryobiology

authors： El-Wahsh M,Fuller B,Davidson B,Rolles K

更新日期：2003-10-01 00:00:00

abstract：:The crystallization of ice from aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using differential scanning calorimetry. In particular, the ice crystallization behavior of glycerol and Me2SO solutions containing approximately the same mole percent solute concentration (i.e., approximately...

journal_title：Cryobiology

authors： Hey JM,Macfarlane DR

更新日期：1996-04-01 00:00:00