Gas exchange measurement as a non-destructive viability assay for frozen-thawed, winter-dormant apple buds.


:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. If post-treatment grafting indicates low survival of the harvested population then further collection and study has to be delayed until the next winter season. This study trials the use of a portable gas exchange system able to discriminate between live and dead buds rapidly, with the assay confirmed as non-destructive by subsequent micropropagation. Active respiration was recorded for 85% of a winter-dormant Malus domestica buds population that showed 91% viability when grafted (n = 45). Lethally stressed material gave no false positive results. When micropropagated after respiratory measurement, a population viability of 76% was recorded. There was a significant, positive correlation between respiration and fresh weight for buds of mass >10 mg, from a population with a mean fresh weight of 17 mg.






Vogiatzi C,Rosenqvist E,Grout BWW




Has Abstract


2018-05-06 00:00:00








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    abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...


    pub_type: 杂志文章


    authors: Browne RK,Mahony M,Clulow J

    更新日期:2002-06-01 00:00:00

  • MicroRNA regulation below zero: differential expression of miRNA-21 and miRNA-16 during freezing in wood frogs.

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    pub_type: 杂志文章


    authors: Biggar KK,Dubuc A,Storey K

    更新日期:2009-12-01 00:00:00

  • 0 °C is better?- Thawing temperature optimization study for cancer cryoablation in a mouse model with green fluorescent protein-labeled Lewis lung cancer.

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    pub_type: 杂志文章


    authors: Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

    更新日期:2017-04-01 00:00:00

  • Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm.

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    pub_type: 杂志文章


    authors: Purdy PH,Graham JK

    更新日期:2004-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Sullivan KJ,Storey KB

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    authors: Herrera B,Eisenberg G,Holberndt O,Desco MM,Rábano A,García-Barreno P,Del Cañizo JF

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    pub_type: 杂志文章


    authors: Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

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    authors: Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

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  • Piglets produced by transfer of vitrified porcine embryos after stepwise dilution of cryoprotectants.

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    authors: Kobayashi S,Takei M,Kano M,Tomita M,Leibo SP

    更新日期:1998-02-01 00:00:00

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    pub_type: 杂志文章


    authors: Naderi N,Hajian M,Souri M,Nasr Esfahani MH,Vash NT

    更新日期:2021-01-16 00:00:00

  • Roles of unfrozen fraction, salt concentration, and changes in cell volume in the survival of frozen human erythrocytes.

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    pub_type: 杂志文章


    authors: Mazur P,Cole KW

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  • Non-toxic freezing media to retain the stem cell reserves in adipose tissues.

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    pub_type: 杂志文章


    authors: Shaik S,Wu X,Gimble JM,Devireddy R

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    pub_type: 杂志文章


    authors: Staels S,De Coster P,Vral A,Temmerman L,De Pauw G

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  • Percutaneous ablative cryoimmunotherapy for micrometastaic abscopal effect: No complications.

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    authors: Soule E,Bandyk M,Matteo J

    更新日期:2018-06-01 00:00:00

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    pub_type: 杂志文章


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    pub_type: 杂志文章


    authors: Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

    更新日期:1995-08-01 00:00:00

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    pub_type: 杂志文章


    authors: Wang HS,Kang L

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    pub_type: 杂志文章


    authors: Jin B,Kleinhans FW,Mazur P

    更新日期:2014-06-01 00:00:00

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    pub_type: 杂志文章


    authors: Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

    更新日期:2018-02-01 00:00:00

  • Crystallization of Ice in Aqueous Solutions of Glycerol and Dimethyl Sulfoxide. 1. A Comparison of Mechanisms

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    pub_type: 杂志文章


    authors: Hey JM,Macfarlane DR

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    pub_type: 杂志文章


    authors: Wang CL,Teo KY,Han B

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    authors: Takeo T,Tsutsumi A,Omaru T,Fukumoto K,Haruguchi Y,Kondo T,Nakamuta Y,Takeshita Y,Matsunaga H,Tsuchiyama S,Sakoh K,Nakao S,Yoshimoto H,Shimizu N,Nakagata N

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    pub_type: 杂志文章


    authors: Bale JS,Hansen TN,Nishino M,Baust JG

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    pub_type: 杂志文章


    authors: Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

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    pub_type: 杂志文章


    authors: Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

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    pub_type: 杂志文章


    authors: Solhaug KA,Chowdhury DP,Gauslaa Y

    更新日期:2018-06-01 00:00:00

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    pub_type: 杂志文章


    authors: McKibbin LS,Paraschak DM

    更新日期:1985-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Katkov II,Kim MS,Bajpai R,Altman YS,Mercola M,Loring JF,Terskikh AV,Snyder EY,Levine F

    更新日期:2006-10-01 00:00:00

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    pub_type: 杂志文章


    authors: Gearing J,Malik KP,Matejtschuk P

    更新日期:2010-08-01 00:00:00