Gas exchange measurement as a non-destructive viability assay for frozen-thawed, winter-dormant apple buds.

Abstract:

:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. If post-treatment grafting indicates low survival of the harvested population then further collection and study has to be delayed until the next winter season. This study trials the use of a portable gas exchange system able to discriminate between live and dead buds rapidly, with the assay confirmed as non-destructive by subsequent micropropagation. Active respiration was recorded for 85% of a winter-dormant Malus domestica buds population that showed 91% viability when grafted (n = 45). Lethally stressed material gave no false positive results. When micropropagated after respiratory measurement, a population viability of 76% was recorded. There was a significant, positive correlation between respiration and fresh weight for buds of mass >10 mg, from a population with a mean fresh weight of 17 mg.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Vogiatzi C,Rosenqvist E,Grout BWW

doi

10.1016/j.cryobiol.2018.05.001

subject

Has Abstract

pub_date

2018-05-06 00:00:00

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(18)30061-0

pub_type

杂志文章
  • A comparison of sucrose, saline, and saline with egg-yolk diluents on the cryopreservation of cane toad (Bufo marinus) sperm.

    abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/s0011-2240(02)00031-7

    authors: Browne RK,Mahony M,Clulow J

    更新日期:2002-06-01 00:00:00

  • MicroRNA regulation below zero: differential expression of miRNA-21 and miRNA-16 during freezing in wood frogs.

    abstract::Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2009.08.009

    authors: Biggar KK,Dubuc A,Storey K

    更新日期:2009-12-01 00:00:00

  • 0 °C is better?- Thawing temperature optimization study for cancer cryoablation in a mouse model with green fluorescent protein-labeled Lewis lung cancer.

    abstract:PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2017.01.008

    authors: Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

    更新日期:2017-04-01 00:00:00

  • Effect of cholesterol-loaded cyclodextrin on the cryosurvival of bull sperm.

    abstract::Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2003.12.001

    authors: Purdy PH,Graham JK

    更新日期:2004-02-01 00:00:00

  • Environmental stress responsive expression of the gene li16 in Rana sylvatica, the freeze tolerant wood frog.

    abstract::Wood frogs (Rana sylvatica) can endure weeks of subzero temperature exposure during the winter with up to 65% of their body water frozen as extracellular ice. Associated with freezing survival is elevated expression of a number of genes/proteins including the unidentified gene, li16, first described in liver. The curr...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2012.01.008

    authors: Sullivan KJ,Storey KB

    更新日期:2012-06-01 00:00:00

  • Paradoxical effects of temperature on vascular tone.

    abstract::Temperature may have significant influence on vascular tone in such cases as organ preservation, coronary bypass surgery, and extracorporeal circulation. The aim of this research was to study the direct effect of temperature variation on vascular tone in an attempt to elucidate the mechanisms involved. In a first seri...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.2000.2263

    authors: Herrera B,Eisenberg G,Holberndt O,Desco MM,Rábano A,García-Barreno P,Del Cañizo JF

    更新日期:2000-08-01 00:00:00

  • The state of T cells before cryopreservation: Effects on post-thaw proliferation and function.

    abstract:AIM:We aim to assess the effect of the state of T cells before cryopreservation on the post-thaw proliferative capacity, phenotype and functional response. METHODS:Peripheral blood mononuclear cells (PBMCs) were isolated from a hepatocellular carcinoma (HCC) patient, and the T cells were frozen during cell culture acc...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2017.08.008

    authors: Luo Y,Wang P,Liu H,Zhu Z,Li C,Gao Y

    更新日期:2017-12-01 00:00:00

  • Survival of mammalian cells under high vacuum condition for ion bombardment.

    abstract::An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2004.08.003

    authors: Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

    更新日期:2004-12-01 00:00:00

  • Piglets produced by transfer of vitrified porcine embryos after stepwise dilution of cryoprotectants.

    abstract::A total of 498 porcine embryos at various stages of development collected from superovulated gilts was used to investigate cryopreservation. First, blastocysts (BL), expanded blastocysts (ExB), and hatched blastocysts (HB) were used to determine the effect of exposure to concentrated solutions of ethylene glycol as cr...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1997.2056

    authors: Kobayashi S,Takei M,Kano M,Tomita M,Leibo SP

    更新日期:1998-02-01 00:00:00

  • Ferulago angulata extract improves the quality of buck spermatozoa post-thaw and counteracts the harmful effects of diazinon and lead.

    abstract::During cryopreservation, spermatozoa are exposed to chemical or physical stress that has adverse effects on the quality of mammalian spermatozoa. Recently, much attention has been paid to environmental contaminants (ECs) in livestock, because of their detrimental effects on livestock productivity and fertility. ECs li...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2021.01.008

    authors: Naderi N,Hajian M,Souri M,Nasr Esfahani MH,Vash NT

    更新日期:2021-01-16 00:00:00

  • Roles of unfrozen fraction, salt concentration, and changes in cell volume in the survival of frozen human erythrocytes.

    abstract::The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(89)90030-8

    authors: Mazur P,Cole KW

    更新日期:1989-02-01 00:00:00

  • Non-toxic freezing media to retain the stem cell reserves in adipose tissues.

    abstract::Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2020.07.005

    authors: Shaik S,Wu X,Gimble JM,Devireddy R

    更新日期:2020-10-01 00:00:00

  • An experimental study on periodontal regeneration after subcutaneous transplantation of rat molar with and without cryopreservation: an in vivo study.

    abstract::This study analysed the effects of cryopreservation on periodontal regeneration of autotransplanted rat molars. First and second maxillary molars (n=92) of 24 four-week-old Wistar rats were gently extracted and autotransplanted into the abdominal tissue immediately (control group n=44) or after cryopreservation in liq...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2013.03.008

    authors: Staels S,De Coster P,Vral A,Temmerman L,De Pauw G

    更新日期:2013-06-01 00:00:00

  • Percutaneous ablative cryoimmunotherapy for micrometastaic abscopal effect: No complications.

    abstract::Recent studies have shown efficacy of nivolumab, a monoclonal antibody specific for an immunoregulatory protein termed programmed death 1 (PD-1), against metastatic renal cell carcinoma. PD-1 is a transmembrane protein expressed on T cells that suppresses activation upon binding to its ligands, which may be secreted p...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2018.04.013

    authors: Soule E,Bandyk M,Matteo J

    更新日期:2018-06-01 00:00:00

  • Effect of the desiccation tolerance and cryopreservation methods on the viability of Citrus limon L. Burm cv. Eureka seeds.

    abstract::Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2019.05.006

    authors: Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

    更新日期:2019-08-01 00:00:00

  • Pre-grafting histological studies of skin grafts cryopreserved in α helix antarctic yeast oriented antifreeze peptide (Afp1m).

    abstract::A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2019.09.012

    authors: Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

    更新日期:2020-02-01 00:00:00

  • Isolation of ice-nucleating active bacteria from the freeze-tolerant frog, Rana sylvatica.

    abstract::Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1995.1036

    authors: Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

    更新日期:1995-08-01 00:00:00

  • Effect of cooling rates on the cold hardiness and cryoprotectant profiles of locust eggs.

    abstract::To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2005.07.003

    authors: Wang HS,Kang L

    更新日期:2005-10-01 00:00:00

  • Survivals of mouse oocytes approach 100% after vitrification in 3-fold diluted media and ultra-rapid warming by an IR laser pulse.

    abstract::Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2014.03.005

    authors: Jin B,Kleinhans FW,Mazur P

    更新日期:2014-06-01 00:00:00

  • Effect of two cooling protocols on the post-thaw characteristics of Iberian ibex sperms.

    abstract::The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2018.01.003

    authors: Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

    更新日期:2018-02-01 00:00:00

  • Crystallization of Ice in Aqueous Solutions of Glycerol and Dimethyl Sulfoxide. 1. A Comparison of Mechanisms

    abstract::The crystallization of ice from aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using differential scanning calorimetry. In particular, the ice crystallization behavior of glycerol and Me2SO solutions containing approximately the same mole percent solute concentration (i.e., approximately...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.1996.0021

    authors: Hey JM,Macfarlane DR

    更新日期:1996-04-01 00:00:00

  • An amino acidic adjuvant to augment cryoinjury of MCF-7 breast cancer cells.

    abstract::One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2008.05.007

    authors: Wang CL,Teo KY,Han B

    更新日期:2008-08-01 00:00:00

  • Establishment of a transport system for mouse epididymal sperm at refrigerated temperatures.

    abstract::The exchange of genetically engineered mouse strains between research facilities requires transporting fresh mouse sperm under refrigerated temperatures. Although sperm generally maintains fertility for 48 h at cold temperatures, in vitro fertilization rates of C57BL/6 mouse sperm are low after 48-h cold storage. Furt...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2012.06.002

    authors: Takeo T,Tsutsumi A,Omaru T,Fukumoto K,Haruguchi Y,Kondo T,Nakamuta Y,Takeshita Y,Matsunaga H,Tsuchiyama S,Sakoh K,Nakao S,Yoshimoto H,Shimizu N,Nakagata N

    更新日期:2012-12-01 00:00:00

  • Effect of cooling rate on the survival of larvae, pupariation, and adult emergence of the gallfly Eurosta solidaginis.

    abstract::Freeze-tolerant third instar larvae of the gallfly Eurosta solidaginis were cooled at 10, 5, 1, and 0.1 degrees C min-1 to -40 degrees C and then warmed to +5 degrees C at 1 degree C min-1. After cooling and warming the larvae were transferred to 21 degrees C and the survival of larvae, success of pupariation, and adu...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(89)90024-2

    authors: Bale JS,Hansen TN,Nishino M,Baust JG

    更新日期:1989-06-01 00:00:00

  • Sperm cryopreservation in guppies and black mollies--a generalized freezing protocol for livebearers in Poeciliidae.

    abstract::In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2009.09.011

    authors: Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

    更新日期:2009-12-01 00:00:00

  • Effects of high-molecular-weight cryoprotectants on platelets and the coagulation system.

    abstract::The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1006/cryo.2000.2247

    authors: Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

    更新日期:2000-06-01 00:00:00

  • Short- and long-term freezing effects in a coastal (Lobaria virens) versus a widespread lichen (L. pulmonaria).

    abstract::Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2018.03.007

    authors: Solhaug KA,Chowdhury DP,Gauslaa Y

    更新日期:2018-06-01 00:00:00

  • An investigation on the use of cryosurgery for treatment of bone spavin, splint, and fractured splint bone injuries in standardbred horses.

    abstract::Bone spavin, splint, and fractured splint bone injuries have been treated with varying methodologies at Wheatley Hall Farm Equine Clinic. Cryosurgery is the most successful. With cryosurgery the small, pain-producing afferent C fibers are destroyed, and painful neuromas do not return. Injured sites were cryosurgically...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/0011-2240(85)90158-0

    authors: McKibbin LS,Paraschak DM

    更新日期:1985-10-01 00:00:00

  • Cryopreservation by slow cooling with DMSO diminished production of Oct-4 pluripotency marker in human embryonic stem cells.

    abstract::We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2006.05.005

    authors: Katkov II,Kim MS,Bajpai R,Altman YS,Mercola M,Loring JF,Terskikh AV,Snyder EY,Levine F

    更新日期:2006-10-01 00:00:00

  • Use of dynamic mechanical analysis (DMA) to determine critical transition temperatures in frozen biomaterials intended for lyophilization.

    abstract::Dynamic mechanical analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analy...

    journal_title:Cryobiology

    pub_type: 杂志文章

    doi:10.1016/j.cryobiol.2010.04.002

    authors: Gearing J,Malik KP,Matejtschuk P

    更新日期:2010-08-01 00:00:00