Abstract：
:Boar spermatozoa are extremely sensitive to low temperatures and the cryopreservation causes dramatic changes in sperm survivability, but it is not clear which part of the cryopreservation process affects the most. The aim of this work was to assess early events of apoptotic changes as damage indicators in boar sperm cooled to 5 °C and exposed to different glycerol (GLY) concentrations. For this purpose, progressive sperm motility (CASA), plasmatic and acrosome membranes integrity (CFDA/PI; phase contrast), plasma membrane functionality (HOS), phosphatidylserine translocation (Annexin-V/FITC) and reduction of mitochondrial membrane potential (Ψm) (JC-10) were carried out at 37 °C, 17 °C and 5 °C in eight boar sperm pools. Afterwards, three aliquots were diluted in different freezing extenders (control: 0% GLY; A: 2% GLY and B: 3% GLY); sperm quality and early apoptotic changes were assessed. Motility was negatively affected during cooling to 5 °C. Furthermore, plasma membrane functionality was the most affected by cooling. The number of necrotic cells was higher at 5 °C. However, no differences were observed in phosphatidylserine translocation. The extender with 3% GLY at 5 °C presented better Ψm than 0 and 2% GLY. Based on this analysis, boar sperm cooling to 5 °C does not modify the rate of early apoptotic changes, although alterations in the Ѱm were evident.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Malcervelli DM,Torres P,Suhevic JF,Cisale H,Fischman MLdoi
10.1016/j.cryobiol.2020.05.012subject
Has Abstractpub_date
2020-08-01 00:00:00pages
97-102eissn
0011-2240issn
1090-2392pii
S0011-2240(19)30622-4journal_volume
95pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1006/cryo.1997.2014
更新日期：1997-06-01 00:00:00
abstract：:The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2077
更新日期：1998-05-01 00:00:00
abstract：:Percutaneous ablation is the currently preferred locoregional therapy for non-resectable hepatocellular cancer (HCC). Cryoablation is an attractive option because it forms an ice ball viewable by many imaging methods. This study assessed the therapeutic effect of comprehensive cryoablation (of intra- and extrahepatic ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2012.11.008
更新日期：2013-02-01 00:00:00
abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.07.006
更新日期：2015-10-01 00:00:00
abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.2001.2306
更新日期：2001-03-01 00:00:00
abstract：:The development of efficient and robust methods for the cryopreservation of human embryonic stem cells (hESCs) is important for the production of master and working cell banks for future clinical applications. Such methods must meet requirements of good manufacturing practice (GMP) and maintain genetic stability of th...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2010.03.007
更新日期：2010-06-01 00:00:00
abstract：:Tambaqui (Colossoma macropomum) is a freshwater fish of great importance to aquaculture in several South American countries. Recent studies have developed a protocol for semen cryopreservation in 0.25 and 0.5 mL straws; however, this technique has limitations for fingerling production at a large scale due to the high ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.02.004
更新日期：2015-04-01 00:00:00
abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2011.12.001
更新日期：2012-04-01 00:00:00
abstract：:A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.07.001
更新日期：2014-08-01 00:00:00
abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2004.06.004
更新日期：2004-10-01 00:00:00
abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2019.09.012
更新日期：2020-02-01 00:00:00
abstract：:The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection. They were then treated with a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1996.0011
更新日期：1996-02-01 00:00:00
abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.10.003
更新日期：2010-04-01 00:00:00
abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.01.002
更新日期：2014-04-01 00:00:00
abstract：:A significant proportion of couples at reproductive age rely on assisted reproductive technology to overcome infertility. In vitro fertilisation (IVF) involves typically the use of exogenous gonadotropins to stimulate the ovary to produce oocytes, which are collected surgically. After fertilization by conventional IVF...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2019.11.041
更新日期：2020-02-01 00:00:00
abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2181
更新日期：1999-06-01 00:00:00
abstract：PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2021.01.005
更新日期：2021-01-02 00:00:00
abstract：:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.05.001
更新日期：2018-05-06 00:00:00
abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.11.002
更新日期：2010-04-01 00:00:00
abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1995.1036
更新日期：1995-08-01 00:00:00
abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(91)90026-k
更新日期：1991-06-01 00:00:00
abstract：:Hypothermia was induced in European hamsters (hibernators) and Wistar rats (nonhibernators), and changes in the firing rate and spike duration of extracellularly recorded action potentials were investigated in hypothalamic slices in vitro. At slice temperatures close to normal body temperature (37 +/- 3 degreesC), 32 ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2122
更新日期：1998-11-01 00:00:00
abstract：:The effects of rapid cooling of semen (cold shock) from 30 degrees C to various temperatures above 0 degrees C on survival of ram spermatozoa suspended in diluents with or without egg yolk were assessed before and after freezing. Rapid cooling of extended semen from 30 to 15 degrees C had little or no effect on sperma...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(86)90061-1
更新日期：1986-12-01 00:00:00
abstract：:We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2006.05.005
更新日期：2006-10-01 00:00:00
abstract：:The performance of a small-scale automated cryopreservation and storage system (Mini-BioArchive system) used in the banking of umbilical cord blood (UCB) units was evaluated. After thawing the units, the viability and recovery of cells, as well as the recovery rate of hematopoietic progenitor cells (HPCs) such as CD34...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2008.07.004
更新日期：2008-10-01 00:00:00
abstract：:The objective of this study was to evaluate in vitro fertilization and cleavage rates of frozen-thawed bovine oocytes at the germinal vesicle (GV) stage. In mouse oocytes, spindle microtubule reorganization after GV breakdown is particularly sensitive to cold and readily damaged by exposure to low temperatures, the da...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1996.0055
更新日期：1996-10-01 00:00:00
abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/s0011-2240(03)00066-x
更新日期：2003-08-01 00:00:00
abstract：:Maintenance of membrane fluidity is of crucial importance in ectotherms experiencing thermal changes. This maintenance has in ectotherms most often been indicated using indirect measures of biochemical changes of phospholipid membranes, which is then assumed to modulate the physico-chemical properties of the membrane....
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.09.164
更新日期：2013-12-01 00:00:00
abstract：:Liver transplantation is currently the preferred treatment option for end-stage liver disease. Donation after cardiac death was a common practice in the early years of organ donation before brain death criteria were established. Those organs were subjected to variable periods of warm ischemia that might intensify cold...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2015.06.006
更新日期：2015-08-01 00:00:00
abstract：:Over recent years, a considerable increase in the popularity of cryostimulation and whole body cryotherapy (WBC) procedures has occurred both among healthy individuals and in various groups of patients, including those with primary untreated hypertension. The aim of this study was to compare the effects of WBC on the ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.07.014
更新日期：2014-10-01 00:00:00
