Abstract:
:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the adequate container for embryo loading, and the temperature for thawing, were the parameters considered at different developmental stages in the present study. After vitrification, embryo morphology was evaluated under stereoscopic microscopy, establishing the percentage of intact embryos. Two of the studied parameters yielded differences in this percentage, the volume of straw used for embryo loading (1 ml straws were significantly better than 0.5 ml straws, with regard to post-thawed embryo morphologies), and the thawing temperature, achieving 49% of embryos with intact morphology after thawing at 0 degrees C. After thawing, the intact embryos were incubated and periodically observed to detect morphological changes. Changes in the perivitelline space, shrinkage of the yolk and chorion ruptures as well as a progressive whitening of the embryo and yolk were observed. After 8 h all embryos showed clear signs of degradation and during this incubation period no embryo showed any developmental ability.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Robles V,Cabrita E,Real M,Alvarez R,Herráez MPdoi
10.1016/s0011-2240(03)00066-xsubject
Has Abstractpub_date
2003-08-01 00:00:00pages
30-9issue
1eissn
0011-2240issn
1090-2392pii
S001122400300066Xjournal_volume
47pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::We have shown previously that the survival of human red blood cells during slow freezing at 2% hematocrit is dependent more on the magnitude of the unfrozen fraction than on the salt concentration in that unfrozen fraction. In parallel, first Nei and more recently Pegg and colleagues have shown that survival is affect...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(85)90029-x
更新日期:1985-12-01 00:00:00
abstract::Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(89)90046-1
更新日期:1989-04-01 00:00:00
abstract::Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice melt samples contained about 3×10(3) - 1×10(4) colony forming unit (CFUs) per ml. Sequence analysis of the isolates, using D1/D2 domain identified five species of yeasts: Cryptococcus adeliensis (MLB-18 JX192655), Cryptoco...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.01.002
更新日期:2013-04-01 00:00:00
abstract::Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90031-7
更新日期:1983-08-01 00:00:00
abstract::The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.01.003
更新日期:2018-02-01 00:00:00
abstract::The development of drugs in cardiovascular research involves in vitro studies that are generally performed on freshly isolated blood vessels. Naturally, data obtained from human tissues would be the most accurate and predictable for human pharmacology. However, the main problems are that the supply of fresh human mate...
journal_title:Cryobiology
pub_type: 杂志文章,评审
doi:10.1006/cryo.1994.1007
更新日期:1994-02-01 00:00:00
abstract::Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cry...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.07.008
更新日期:2018-10-01 00:00:00
abstract::Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.08.003
更新日期:2011-12-01 00:00:00
abstract::Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2199
更新日期:1999-09-01 00:00:00
abstract::Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.06.003
更新日期:2013-10-01 00:00:00
abstract::The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.11.007
更新日期:2009-04-01 00:00:00
abstract::Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2010.10.158
更新日期:2010-12-01 00:00:00
abstract::We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1994.1023
更新日期:1994-04-01 00:00:00
abstract::Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.06.007
更新日期:2006-10-01 00:00:00
abstract::The purpose of our study was to investigate underlying basic mechanisms of hypothermia-induced cardioprotection during oxidative stress in a cardiomyocyte cell culture model. For hypothermic treatment we cooled H9c2 cardiomyocytes to 20°C, maintained 20min at 20°C during which short-term oxidative damage was inflicted...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2010.12.003
更新日期:2011-02-01 00:00:00
abstract::Morulae and unhatched blastocysts from Large White hyperprolific (LWh) and Meishan (MS) gilts were selected to test an ultrarapid open pulled straw (OPS) vitrification method with two media. The viability of vitrified/warmed embryos was estimated by the percentage of embryos that developed to the hatched blastocyst st...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2273
更新日期:2000-09-01 00:00:00
abstract::Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2016.08.001
更新日期:2016-10-01 00:00:00
abstract::We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2017.12.005
更新日期:2018-02-01 00:00:00
abstract::The effect of low temperatures on the ultrastructure of the plasma membrane of bull and boar spermatozoa was investigated. Cold-induced changes in the organization of sperm plasma membrane components were demonstrated by the use of fast-freezing combined with freeze-fracture electron microscopy. This preparation techn...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(90)90009-s
更新日期:1990-04-01 00:00:00
abstract::Wood frogs (Rana sylvatica) can endure weeks of subzero temperature exposure during the winter with up to 65% of their body water frozen as extracellular ice. Associated with freezing survival is elevated expression of a number of genes/proteins including the unidentified gene, li16, first described in liver. The curr...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2012.01.008
更新日期:2012-06-01 00:00:00
abstract::(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(87)90054-x
更新日期:1987-12-01 00:00:00
abstract::The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2009.07.011
更新日期:2010-02-01 00:00:00
abstract::Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2019.09.001
更新日期:2019-10-01 00:00:00
abstract::Primary cell line cultures from human skin biopsies, adipose tissue and tumor tissue are valuable samples for research and therapy. In this regard, their derivation, culture, storage, transport and thawing are important steps to be studied. Towards this end, we wanted to establish the derivation, and identify the cult...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.10.011
更新日期:2015-02-01 00:00:00
abstract::The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2147
更新日期:1999-02-01 00:00:00
abstract::Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.05.001
更新日期:2018-05-06 00:00:00
abstract::To examine the relationship between cooling rate and cold hardiness in eggs of the migratory locust, Locusta migratoria, the survival rates and cryoprotectant levels of three embryonic developmental stages were measured at different cooling rates (from 0.05 to 0.8 degrees C min(-1)) in acclimated and non-acclimated eg...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.07.003
更新日期:2005-10-01 00:00:00
abstract::Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.03.005
更新日期:2014-06-01 00:00:00
abstract::In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.01.013
更新日期:2011-04-01 00:00:00
abstract::We have previously shown that cold-induced injury to hepatocytes and liver endothelial cells occurs predominantly via an iron-dependent pathway. However, other groups have reported evidences suggesting that Ca(2+) ions could be involved in the process of cold-induced injury of liver cells. We here assessed the relativ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2007.11.002
更新日期:2008-04-01 00:00:00