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Defining the optimal cryoprotectant and concentration for cryopreservation of limbal stem cells.

Abstract：

:Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cryoprotectant concentration for LSC cultures. Suspension cultures derived from cadaveric corneoscleral rims were cooled to 4 °C with Me2SO, propylene glycol or ethylene glycol at a concentration of 5%, 10% or 15%. Cell tolerance was measured in terms of membrane integrity, colony-forming efficiency and alamarBlue® reduction. Increasing cryoprotectant concentration above 5% reduced membrane integrity, metabolism and colony-forming efficiency. Cryoprotectant choice did not significantly influence these characteristics. Cells demonstrating Side Population were maintained after cryopreservation with 5% propylene glycol in vapour phase liquid nitrogen for 1 week, indicating that cryopreservation of LSCs with relatively low cryoprotectant concentration (5%) has promise in low-temperature eye banking.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Osei-Bempong C,Ghareeb AE,Lako M,Figueiredo FC,Armitage WJ

doi

10.1016/j.cryobiol.2018.07.008

subject

Has Abstract

pub_date

2018-10-01 00:00:00

pages

98-102

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(18)30086-5

journal_volume

84

pub_type

杂志文章

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