Improved recovery of post-thaw motility and vitality of human spermatozoa cryopreserved in the presence of dithiothreitol.

abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

journal_title：Cryobiology

authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

更新日期：2020-12-04 00:00:00

abstract：:The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...

journal_title：Cryobiology

authors： Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

更新日期：2000-06-01 00:00:00

abstract：:Hypothermic machine perfusion (HMP) and static cold storage (SCS) are the two methods used to preserve deceased donor kidneys prior to transplant. This study seeks to characterise the metabolic profile of HMP and SCS porcine kidneys in a cardiac death donor model. Twenty kidneys were cold flushed and stored for two ho...

journal_title：Cryobiology

authors： Nath J,Smith TB,Patel K,Ebbs SR,Hollis A,Tennant DA,Ludwig C,Ready AR

更新日期：2017-02-01 00:00:00

abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...

journal_title：Cryobiology

authors： Slotsbo S,Maraldo K,Malmendal A,Nielsen NC,Holmstrup M

更新日期：2008-12-01 00:00:00

abstract：:Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...

journal_title：Cryobiology

authors： Lee S,Iwasaki Y,Yoshizaki G

更新日期：2016-10-01 00:00:00

abstract：:Organ preservation solutions have been designed to protect grafts against the injury inflicted by cold ischemia. However, toxicity of University of Wisconsin (UW) solution during rewarming has been reported. Therefore, we here assessed the toxicity of UW, histidine-tryptophan-ketoglutarate (HTK), Euro-Collins, histidi...

journal_title：Cryobiology

authors： Rauen U,de Groot H

更新日期：2008-02-01 00:00:00

abstract：:Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol. Fifteen mi...

journal_title：Cryobiology

authors： Takahashi T,Hirsh A,Erbe EF,Bross JB,Steere RL,Williams RJ

更新日期：1986-04-01 00:00:00

abstract：:Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...

journal_title：Cryobiology

authors： Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng X

更新日期：2013-12-01 00:00:00

abstract：:One thousand cryohemorrhoidectomies were performed in the past 7 years (1973-1979). Ninety percent of the patients were satisfied with the technique; approximately one-third of the patients experienced severe to moderate pain and 11% had recurrences or related problems. The majority of patients with postoperative pain...

journal_title：Cryobiology

authors： Oh C

更新日期：1982-06-01 00:00:00

abstract：OBJECTIVE:Liver metastases occur in approximately 4%-14% of gastric cancer patients and are associated with high mortality. However, no standardized treatment approach is available for these patients. We aimed to assess the clinical outcomes of patients with gastric cancer liver metastases (GCLM) who underwent percutan...

journal_title：Cryobiology

authors： Chang X,Wang Y,Yu HP,Zhang WH,Yang XL,Guo Z

更新日期：2018-06-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:In vitro testing of human liver for biotransformation or xenobiotic metabolism studies has been limited by unpredictable acquisition of samples. Consequently, it has become necessary to consider methods to cryopreserve and store these samples whenever they do become available for culture of the revived tissue at a mor...

journal_title：Cryobiology

authors： Wishnies SM,Parrish AR,Sipes IG,Gandolfi AJ,Putnam CW,Krumdieck CL,Brendel K

更新日期：1991-06-01 00:00:00

abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...

journal_title：Cryobiology

authors： Benson JD

更新日期：2012-04-01 00:00:00

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:Ten strains of cryophilic yeast were studied from glacier ice cores of Svalbard, Arctic. The ice melt samples contained about 3×10(3) - 1×10(4) colony forming unit (CFUs) per ml. Sequence analysis of the isolates, using D1/D2 domain identified five species of yeasts: Cryptococcus adeliensis (MLB-18 JX192655), Cryptoco...

journal_title：Cryobiology

authors： Singh P,Tsuji M,Singh SM,Roy U,Hoshino T

更新日期：2013-04-01 00:00:00

abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

journal_title：Cryobiology

authors： Karow AM Jr,Jeske AH

更新日期：1976-08-01 00:00:00

abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

journal_title：Cryobiology

authors： Stott SL,Karlsson JOM

更新日期：2009-02-01 00:00:00

abstract：:Cryopreservation by low-cryoprotectant (CPA) vitrification has the potential to combine all the advantages of the conventional high-CPA vitrification and slow-freezing approaches while avoiding their drawbacks. However, current low-CPA vitrification protocol for cryopreservation of oocytes requires a lengthy and multi...

journal_title：Cryobiology

authors： Choi JK,Huang H,He X

更新日期：2015-06-01 00:00:00

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:Maintenance of membrane fluidity is of crucial importance in ectotherms experiencing thermal changes. This maintenance has in ectotherms most often been indicated using indirect measures of biochemical changes of phospholipid membranes, which is then assumed to modulate the physico-chemical properties of the membrane....

journal_title：Cryobiology

authors： Waagner D,Bouvrais H,Ipsen JH,Holmstrup M

更新日期：2013-12-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00

abstract：:Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central ne...

journal_title：Cryobiology

authors： Jensen S,Sørensen T,Zimmer J

更新日期：1987-04-01 00:00:00

abstract：:Wood frogs (Rana sylvatica) can endure weeks of subzero temperature exposure during the winter with up to 65% of their body water frozen as extracellular ice. Associated with freezing survival is elevated expression of a number of genes/proteins including the unidentified gene, li16, first described in liver. The curr...

journal_title：Cryobiology

authors： Sullivan KJ,Storey KB

更新日期：2012-06-01 00:00:00

abstract：:Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and ...

journal_title：Cryobiology

authors： Puts CF,Berendsen TA,Bruinsma BG,Ozer S,Luitje M,Usta OB,Yarmush ML,Uygun K

更新日期：2015-08-01 00:00:00

abstract：:The process of ice formation and propagation during cryopreservation impacts on the post-thaw outcome for a sample. Two processes, either network solidification or progressive solidification, can dominate the water-ice phase transition with network solidification typically present in small sample cryo-straws or cryo-v...

journal_title：Cryobiology

authors： Kilbride P,Morris GJ,Milne S,Fuller B,Skepper J,Selden C

更新日期：2014-12-01 00:00:00

abstract：:In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...

journal_title：Cryobiology

authors： Kusuda S,Teranishi T,Koide N

更新日期：2002-08-01 00:00:00

abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...

journal_title：Cryobiology

authors： Huang KY,de Groot SA,Woelders H,van der Horst GT,Themmen AP,Colenbrander B,van Vlissingen JM

更新日期：2010-04-01 00:00:00

abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

journal_title：Cryobiology

authors： Hao YJ,Liu QL,Deng XX

更新日期：2001-08-01 00:00:00

abstract：:The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...

journal_title：Cryobiology

authors： Song WJ,Jiji LM

更新日期：1988-04-01 00:00:00

abstract：:Liver transplantation is currently the preferred treatment option for end-stage liver disease. Donation after cardiac death was a common practice in the early years of organ donation before brain death criteria were established. Those organs were subjected to variable periods of warm ischemia that might intensify cold...

journal_title：Cryobiology

authors： Balaban CL,Rodríguez JV,Tiribelli C,Guibert EE

更新日期：2015-08-01 00:00:00