Abstract:
:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attachment to reproducible circular or rectangular domains. Employing frame rates of 8000 frames/s and 16,000 frames/s to record IIF in micropatterned and suspended cells, respectively, intracellular crystal growth manifested as a single advancing front that initiated from a point source within the cell, and traveled at velocities of 0.0006-0.023 m/s. Whereas this primary crystallization process resulted in minimal change in cell opacity, the well-known flashing phenomenon (i.e., cell darkening) was shown to be a secondary event that does not occur until after the ice front has traversed the cell. In cells that were attached and spread on a substrate, IIF initiation sites were preferentially localized to the peripheral zone of the adherent cells. This non-uniformity in the spatial distribution of crystal centers contradicts predictions based on common theories of IIF, and provides evidence for a novel mechanism of IIF in adherent cells. A second IIF mechanism was evident in approximately 20% of attached cells. In these cases, IIF was preceded by paracellular ice penetration; the initiation site of the subsequent IIF event was correlated with the location of the paracellular ice dendrite, indicating an association (and possibly a causal relationship) between the two. Together, the peripheral-zone and dendrite-associated initiation mechanisms accounted for 97% of IIF events in micropatterned cells.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Stott SL,Karlsson JOMdoi
10.1016/j.cryobiol.2008.11.003subject
Has Abstractpub_date
2009-02-01 00:00:00pages
84-95issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(08)00277-0journal_volume
58pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2339
更新日期:2001-08-01 00:00:00
abstract::The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2221
更新日期:2000-02-01 00:00:00
abstract:BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2015.07.006
更新日期:2015-10-01 00:00:00
abstract::Development of in vitro culture protocol for early stage ovarian follicles of zebrafish is important since cryopreserved early stage ovarian follicles would need to be matured in vitro following cryopreservation before they can be fertilised. Development of molecular markers for zebrafish (Danio rerio) ovarian follicl...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.05.004
更新日期:2018-08-01 00:00:00
abstract::An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.08.003
更新日期:2004-12-01 00:00:00
abstract::Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2085
更新日期:1998-06-01 00:00:00
abstract::A 9 kDA antifreeze protein (AFP) was isolated and purified from the Antarctic springtail, Gomphiocephalus hodgsoni. By combining selective sampling procedures and a modified ice affinity purification protocol it was possible to directly isolate a single AFP protein without recourse to chromatographic separation techni...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.07.001
更新日期:2014-08-01 00:00:00
abstract::Twenty-eight-day-old male rats were used in three experiments to study whether cold exposure potentiates pineal actions in nonhibernating mammals. The following questions were considered: (a) Can cold exposure increase the antigonadal effects of light deprivation? (b) Are the effects induced by blindness plus cold exp...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(86)90020-9
更新日期:1986-02-01 00:00:00
abstract::Boar spermatozoa are extremely sensitive to low temperatures and the cryopreservation causes dramatic changes in sperm survivability, but it is not clear which part of the cryopreservation process affects the most. The aim of this work was to assess early events of apoptotic changes as damage indicators in boar sperm ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.05.012
更新日期:2020-08-01 00:00:00
abstract:PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2021.01.005
更新日期:2021-01-02 00:00:00
abstract::Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...
journal_title:Cryobiology
pub_type: 杂志文章,评审
doi:10.1006/cryo.2001.2359
更新日期:2001-09-01 00:00:00
abstract::This study analysed the effects of cryopreservation on periodontal regeneration of autotransplanted rat molars. First and second maxillary molars (n=92) of 24 four-week-old Wistar rats were gently extracted and autotransplanted into the abdominal tissue immediately (control group n=44) or after cryopreservation in liq...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.03.008
更新日期:2013-06-01 00:00:00
abstract::Bull sperm were treated with several levels of cholesterol-loaded cyclodextrin (CLC) and frozen in egg yolk diluents containing either Tris or sodium citrate, to determine the CLC concentration that best benefits bull sperm cryosurvival. After thawing, higher percentages of motile (60%) and viable (55%) sperm were obt...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2003.12.001
更新日期:2004-02-01 00:00:00
abstract::The common carotid artery of the rabbit, a typical small elastic artery, can be cryopreserved using dimethyl sulfoxide, slow cooling, storage at less than -160 degrees C, and rapid warming. This technique provides satisfactory preservation of muscle and endothelial cells, but in about 75% of cases, gross circumferenti...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1994.1061
更新日期:1994-10-01 00:00:00
abstract::Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2012.04.008
更新日期:2012-10-01 00:00:00
abstract::Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.11.007
更新日期:2012-04-01 00:00:00
abstract::Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2098
更新日期:1998-08-01 00:00:00
abstract::Vitrification is the most sought after route to the cryopreservation of animal embryos and oocytes and other cells of medical, genetic, and agricultural importance. Current thinking is that successful vitrification requires that cells be suspended in and permeated by high concentrations of protective solutes and that ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.03.005
更新日期:2014-06-01 00:00:00
abstract::Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application. We previously reported a novel cell transplantation method, in which bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured to confluence and differentiated down th...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.03.011
更新日期:2013-06-01 00:00:00
abstract::Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90031-7
更新日期:1983-08-01 00:00:00
abstract::Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2146
更新日期:1999-05-01 00:00:00
abstract::The purpose of our study was to investigate underlying basic mechanisms of hypothermia-induced cardioprotection during oxidative stress in a cardiomyocyte cell culture model. For hypothermic treatment we cooled H9c2 cardiomyocytes to 20°C, maintained 20min at 20°C during which short-term oxidative damage was inflicted...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2010.12.003
更新日期:2011-02-01 00:00:00
abstract::Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.02.007
更新日期:2005-06-01 00:00:00
abstract::We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2017.05.009
更新日期:2017-08-01 00:00:00
abstract::Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2010.10.158
更新日期:2010-12-01 00:00:00
abstract::This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.01.002
更新日期:2008-04-01 00:00:00
abstract::Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00031-7
更新日期:2002-06-01 00:00:00
abstract::Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2017.01.002
更新日期:2017-04-01 00:00:00
abstract::We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90045-4
更新日期:1988-08-01 00:00:00
abstract::We have measured the effectiveness of different standard cryogenic liquids (liquid nitrogen, chilled isopentane, chilled hexane, and chilled ethanol) and applied a mathematical model for calculating the temperature changes at different depths in rabbit and rat heads cooled in liquid nitrogen. We have also evaluated th...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1997.2032
更新日期:1997-09-01 00:00:00