In situ freezing of the rabbit and rat brain.

Abstract:

:We have measured the effectiveness of different standard cryogenic liquids (liquid nitrogen, chilled isopentane, chilled hexane, and chilled ethanol) and applied a mathematical model for calculating the temperature changes at different depths in rabbit and rat heads cooled in liquid nitrogen. We have also evaluated the extent to which the freezing time is shortened when the skin is removed prior to immersion. We conclude that: (i) of the coolants used, liquid nitrogen gives the most rapid freezing. (ii) dry ice-chilled ethanol is as effective as dry ice-chilled hexane. Liquid nitrogen and ethanol are safer for the environment than isopentane and hexane and should satisfy most needs. (iii) By removing the skin prior to immersion, the freezing time can be reduced by about 20 s for the rabbit cortex and rat hypothalamus, 30 s for the rat cortex, and 40 s for the rabbit hypothalamus.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Roos MW,Johansson A,Sperber GO

doi

10.1006/cryo.1997.2032

subject

Has Abstract

pub_date

1997-09-01 00:00:00

pages

187-91

issue

2

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(97)92032-0

journal_volume

35

pub_type

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