Abstract：
:One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study was to further investigate the extent of oxidative injury during the loading of trehalose into RBCs. RBCs were incubated in the absence (control) or presence of trehalose (0.8 mol/l) at 4 degrees C or 37 degrees C for different time scales. Oxidative damage was monitored by flow cytometry using dichlorofluorescin for reactive oxygen species formation, Annexin V-FITC for phosphatidylserine translocation and fluorescein-DHPE for lipid peroxidation. Percent methemoglobin, percent hemolysis and thiobarbituric acid reactive substances were measured by spectrophotometry. The extent of oxidative damage during trehalose loading is affected by the incubation temperature, incubation time and the presence of trehalose. Incubation at 4 degrees C was relatively innocuous; however, oxidative injury was evident at 37 degrees C in both RBC groups. The addition of trehalose is correlated with high osmotic pressure, which had minor effects during incubation at 4 degrees C, but seemed to have exacerbated the severity of cellular injury at 37 degrees C, as measured by higher levels of hemolysis, methemoglobin and lipid peroxidation. The process of trehalose-loading is problematic due to its requirement for prolonged incubations at 37 degrees C. These conditions are correlated with oxidative injury, even in the absence of trehalose. While trehalose is believed to be crucial for stabilizing biomembranes, the consequences of its introduction into the cells require further investigation.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Kanias T,Acker JPdoi
10.1016/j.cryobiol.2008.12.003subject
Has Abstractpub_date
2009-04-01 00:00:00pages
232-9issue
2eissn
0011-2240issn
1090-2392pii
S0011-2240(08)00314-3journal_volume
58pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract：:We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2006.05.005
更新日期：2006-10-01 00:00:00
abstract：:Mouse oocytes were cryopreserved by the vitrification method using vitrification solution (VSI) and the effects of dilution methods were examined on the rate of in vitro and in vivo development. Eighty-three percent and 75% of vitrified oocytes exhibited normal morphology when diluted in glycerol + sucrose and sucrose...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(91)90007-b
更新日期：1991-02-01 00:00:00
abstract：:The aromatic amino acid l-tryptophan is an essential and versatile molecule, acts by transferring an electron to free radicals and protects the plasma membrane from injuries. The aim of the present study was to investigate the effects of l-tryptophan in extender on semen quality parameters, in vitro longevity and in v...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2019.11.044
更新日期：2020-02-01 00:00:00
abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.03.009
更新日期：2020-08-01 00:00:00
abstract：:Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2098
更新日期：1998-08-01 00:00:00
abstract：:Wood frogs (Rana sylvatica) can endure weeks of subzero temperature exposure during the winter with up to 65% of their body water frozen as extracellular ice. Associated with freezing survival is elevated expression of a number of genes/proteins including the unidentified gene, li16, first described in liver. The curr...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2012.01.008
更新日期：2012-06-01 00:00:00
abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2017.01.001
更新日期：2017-04-01 00:00:00
abstract：:Ethylene glycol (EG) is the emerging cryoprotectant of choice for preservation of mammalian embryos but has not been widely used for oocyte preservation. Techniques for oocyte cryopreservation need to be improved before they can be incorporated into routine clinical practice. Hence the permeability characteristics of ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2199
更新日期：1999-09-01 00:00:00
abstract：:The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(89)90030-8
更新日期：1989-02-01 00:00:00
abstract：:Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.11.001
更新日期：2020-11-10 00:00:00
abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1006/cryo.1997.2014
更新日期：1997-06-01 00:00:00
abstract：:Dipalmitoylphosphatidylcholine (DPPC) bilayers hydrated in the presence of trehalose were equilibrated at various temperatures (4, 20, and 60 degrees C) corresponding to the crystalline Lc, gel L beta', and liquid-crystalline L alpha phases, respectively, and then desiccated at these temperatures or freeze-dried at -8...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(88)90033-8
更新日期：1988-06-01 00:00:00
abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.07.011
更新日期：2010-02-01 00:00:00
abstract：:Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(83)90031-7
更新日期：1983-08-01 00:00:00
abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90005-m
更新日期：1992-02-01 00:00:00
abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2020.04.009
更新日期：2020-06-01 00:00:00
abstract：:Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.10.017
更新日期：2020-11-04 00:00:00
abstract：:The importance of the frozen phase in the formation of cryooscillations of trypsin activity has been shown in experiments conducted at -10 degrees C under frozen and supercooled conditions, respectively. A solution containing trypsin obtained by trypsinogen activation and 0.1 M MnCl2 was distributed in test tubes with...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/0011-2240(92)90055-7
更新日期：1992-08-01 00:00:00
abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2182
更新日期：1999-08-01 00:00:00
abstract：:The effects of the presence or absence of cumulus cells and equilibration times (1 and 4 min) with cryoprotectant, and Taxol treatment before vitrification (0.5-5.0 microM) on development of in vitro matured porcine oocytes after vitrification were examined. Ethylene glycol (30%) and sucrose (0.5M) was used as a vitri...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2005.08.002
更新日期：2005-12-01 00:00:00
abstract：:Grafting of cryopreserved testicular tissue is a promising tool for fertility and testicular function preservation in endangered species, mutant animals, or cancer patients for future use. In this study, we aimed to improve the whole neonatal mouse testicular tissue cryopreservation protocols by comparing cryosurvival...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2018.09.001
更新日期：2018-10-01 00:00:00
abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2014.06.003
更新日期：2014-08-01 00:00:00
abstract：:Antifreeze proteins are characterised by their ability to prevent ice from growing upon cooling below the bulk melting point. This displacement of the freezing temperature of ice is limited and at a sufficiently low temperature a rapid ice growth takes place. The separation of the melting and freezing temperature is u...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2005.07.007
更新日期：2005-12-01 00:00:00
abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2009.11.002
更新日期：2010-04-01 00:00:00
abstract：:Despite the worldwide application of embryo-freezing technology as the means of preserving germplasm of mammalian species, there is no information available on the possible transmission of infectious agents to cryopreserved embryos via contaminated liquid nitrogen (LN). Recently, it has been reported that new methods ...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1999.2227
更新日期：2000-03-01 00:00:00
abstract：:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (B...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2129
更新日期：1998-12-01 00:00:00
abstract：:The cryopreservation of isolated fish blastomeres is likely to provide a valid alternative to embryo cryopreservation, the results of which are still discouraging. A repeatable technique for the cryopreservation of rainbow trout blastomeres has been established and the effect of embryonic developmental stage on freezi...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1006/cryo.1998.2084
更新日期：1998-06-01 00:00:00
abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2020.12.002
更新日期：2020-12-03 00:00:00
abstract：:A significant proportion of couples at reproductive age rely on assisted reproductive technology to overcome infertility. In vitro fertilisation (IVF) involves typically the use of exogenous gonadotropins to stimulate the ovary to produce oocytes, which are collected surgically. After fertilization by conventional IVF...
journal_title：Cryobiology
pub_type： 杂志文章,评审
doi：10.1016/j.cryobiol.2019.11.041
更新日期：2020-02-01 00:00:00
abstract：:The Zebrafish has gained increased popularity as an aquatic model species in various research fields, and its widespread use has led to numerous mutant strains and transgenic lines. This creates the need to store these important genetic materials as frozen gametes. Sperm cryopreservation in zebrafish has been shown to...
journal_title：Cryobiology
pub_type： 杂志文章
doi：10.1016/j.cryobiol.2013.05.011
更新日期：2013-10-01 00:00:00
