Abstract：
:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (Bufo marinus) was undertaken to determine the feasibility of cryopreservation of amphibian sperm. Sperm suspensions for cryopreservation were prepared by macerating testes in cryoprotective additives of 10% (w/v) sucrose or 10% (w/v) sucrose containing either 10, 15, or 20% (v/v) glycerol or 10, 15, or 20% (v/v) dimethyl sulfoxide (Me2SO). Suspensions were then cooled to -85 degreesC using a controlled rate cooler, stored in LN2, and thawed in air. The motility and fertilization rate of cryopreserved suspensions and unfrozen control suspensions in Simplified Amphibian Ringer were compared. Sucrose alone had no cryoprotective effect. All other treatments showed varying degrees of recovery of motility and fertilizing capacity. High rates of recovery of motility and fertilizing capacity were observed with 15% Me2SO (68.9 +/- 3.8 and 60.5 +/- 4.7%) and 20% glycerol (58.0 +/- 5.9 and 81.4 +/- 4.3%), respectively. Motility and fertilization rates were similar with Me2SO but diverged with glycerol as cryoprotectant. The data demonstrate the feasibility of using sperm cryopreservation with amphibian species. Copyright 1998 Academic Press.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Browne RK,Clulow J,Mahony M,Clark Adoi
10.1006/cryo.1998.2129subject
Has Abstractpub_date
1998-12-01 00:00:00pages
339-45issue
4eissn
0011-2240issn
1090-2392pii
S0011-2240(98)92129-0journal_volume
37pub_type
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