Fc receptors and human T lymphocytes in frozen-thawed peripheral blood cells.

abstract：:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was ...

journal_title：Cryobiology

authors： Zhang T,Isayeva A,Adams SL,Rawson DM

更新日期：2005-06-01 00:00:00

abstract：:Attempts were made to develop a simplified procedure for long-term cryopreservation of intestinal smooth muscle cells (ISMC). ISMC were collected from the ileum of Sprague-Dawley neonatal rats through cellular dissociation in trypsin. Cryopreservation method comprised of a rapid 1-step (protocol 1) and a slow 3-step (...

journal_title：Cryobiology

authors： Batista Lobo S,Denyer M,Gopalan RC,Javid FA

更新日期：2008-10-01 00:00:00

abstract：:Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...

journal_title：Cryobiology

authors： Correia LFL,Espírito-Santo CG,Braga RF,Carvalho-de-Paula CJ,da Silva AA,Brandão FZ,Freitas VJF,Ungerfeld R,Souza-Fabjan JMG

更新日期：2020-11-10 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：:The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated ...

journal_title：Cryobiology

authors： Puhlev I,Guo N,Brown DR,Levine F

更新日期：2001-05-01 00:00:00

abstract：:Cold storage (at 4°C) offers a compromise between the benefits and disadvantages of cooling. It allows storage of organs or cells for later use that would otherwise quickly succumb to warm ischemia, but comprises cold ischemia that, when not controlled properly, can result in severe damage as well by both similar and ...

journal_title：Cryobiology

authors： Puts CF,Berendsen TA,Bruinsma BG,Ozer S,Luitje M,Usta OB,Yarmush ML,Uygun K

更新日期：2015-08-01 00:00:00

abstract：:Our previous study revealed that melatonin (MLT) protected the quality of cryopreserved ovarian tissues in mice. This work was carried out to examine the role of MLT in inducing HSP90 expression of ovarian tissue for achieving cryoprotection. Pieces of ovarian tissues were obtained from 50 female rats treated with MLT...

journal_title：Cryobiology

authors： Cheng LY,Sun TC,Liu XC,Yu H,Zhou SJ,Tian L,Yang SH,Liu BX

更新日期：2020-12-03 00:00:00

abstract：:We have studied the sensitivity to cryoprotecting agents of different embryos of the local sea urchin, Echinometra lucunter which is the species used for embryo-larval bioassays in Brazil. We have located significant differences between both species sensitivity to cryoprotecting agents; while for P. lividus propylene ...

journal_title：Cryobiology

authors： Ribeiro MB,Furley T,Spago FR,Paredes E

更新日期：2018-02-01 00:00:00

abstract：:The effect of low temperatures on the ultrastructure of the plasma membrane of bull and boar spermatozoa was investigated. Cold-induced changes in the organization of sperm plasma membrane components were demonstrated by the use of fast-freezing combined with freeze-fracture electron microscopy. This preparation techn...

journal_title：Cryobiology

authors： De Leeuw FE,Chen HC,Colenbrander B,Verkleij AJ

更新日期：1990-04-01 00:00:00

abstract：:This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

journal_title：Cryobiology

authors： Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

更新日期：2017-04-01 00:00:00

abstract：:This paper describes some new approaches for understanding the permeability of teleost embryos. The dechorionated zebrafish (Brachydanio rerio) was used as a model for basic studies of water and cryoprotectant permeability. These embryos are composed of two compartments, a large yolk (surrounded by the yolk syncytial ...

journal_title：Cryobiology

authors： Hagedorn M,Hsu E,Kleinhans FW,Wildt DE

更新日期：1997-06-01 00:00:00

abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...

journal_title：Cryobiology

authors： Kocyigit A,Cevik M

更新日期：2015-08-01 00:00:00

abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

journal_title：Cryobiology

authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

更新日期：2020-11-26 00:00:00

abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

journal_title：Cryobiology

authors： Stott SL,Karlsson JOM

更新日期：2009-02-01 00:00:00

abstract：:Trypsin activity oscillations are shown by the autocatalytic activation of trypsinogen at 0 degrees C in aqueous solution. The oscillations were observed for 3-4 days and show only slight decrease in enzyme activity. The zymogen has been kept at ice water temperature and pH 8.2 in the presence of Mn2+ ion. The mean pe...

journal_title：Cryobiology

authors： Vajda T

更新日期：1986-06-01 00:00:00

abstract：:Antifreeze proteins (AFPs) share two related properties: the ability to depress the freezing temperature below the melting point of ice (thermal hysteresis; TH); and the ability to inhibit the restructuring of ice into larger crystals. Since the 'hyperactive' AFPs, which have been more recently discovered, show an ord...

journal_title：Cryobiology

authors： Yu SO,Brown A,Middleton AJ,Tomczak MM,Walker VK,Davies PL

更新日期：2010-12-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:Cell injury due to osmotic dehydration, which is regarded as a major cause of injury during freeze-thaw processes, was examined closely using a perfusion microscope. Human prostatic adenocarcinoma cells (PC-3), which were put in a chamber, were subjected to hyperosmotic stresses by perfusing NaCl solutions of varying ...

journal_title：Cryobiology

authors： Zawlodzka S,Takamatsu H

更新日期：2005-02-01 00:00:00

abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

journal_title：Cryobiology

authors： Hao YJ,Liu QL,Deng XX

更新日期：2001-08-01 00:00:00

abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...

journal_title：Cryobiology

authors： Shaw JM,Trounson AO

更新日期：1989-10-01 00:00:00

abstract：:A simple method to cryopreserve adherent monolayers of neuronal cells is currently not available, but the development of this technique could facilitate numerous applications in the field of biomedical engineering, cell line development, and drug screening. However, complex tissues of some exceptional animals survive ...

journal_title：Cryobiology

authors： Bailey TL,Wang M,Solocinski J,Nathan BP,Chakraborty N,Menze MA

更新日期：2015-12-01 00:00:00

abstract：:One of the major challenges in cryosurgery is to minimize incomplete cryodestruction near the edge of the iceball. In the present study, the feasibility and effectiveness of an amino acidic adjuvant, glycine was investigated to enhance the cryodestruction of MCF-7 human breast cancer cell at mild freezing/thawing cond...

journal_title：Cryobiology

authors： Wang CL,Teo KY,Han B

更新日期：2008-08-01 00:00:00

abstract：:Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect i...

journal_title：Cryobiology

authors： Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng X

更新日期：2013-12-01 00:00:00

abstract：:Rabbit kidney were perfused at 37degreesC with various concentrations of DMSO in a K+-Mg2+-rich perfusate. The effects of DMSO on various functional parameters of the rabbit kidney perfused for 60 min were compared with the functional effects of perfusion without DMSO under the same conditions. DMSO produced deviation...

journal_title：Cryobiology

authors： Karow AM Jr,Jeske AH

更新日期：1976-08-01 00:00:00

abstract：:The volumetric response of oocytes during rapid alterations of the extracellular osmotic environment were recorded using video microscopy. From these observations, the kinetics of water loss for human and mouse oocytes were determined over the temperature range 37 to 10 degrees C, including 37, 30, 20, and 10 degrees ...

journal_title：Cryobiology

authors： Hunter JE,Bernard A,Fuller BJ,McGrath JJ,Shaw RW

更新日期：1992-04-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...

journal_title：Cryobiology

authors： Tsuji M,Goshima T,Matsushika A,Kudoh S,Hoshino T

更新日期：2013-10-01 00:00:00

abstract：:The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...

journal_title：Cryobiology

authors： Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

更新日期：2006-08-01 00:00:00

abstract：:Cryopreservation of the germplasm for long-term periods is of great importance to maintain the genetic resource. Argentina is one of the world's highest lemon producing country. The performance of different cooling/warming rates in the cryopreservation method of Citrus limon L. Burm cv. Eureka seeds and their influenc...

journal_title：Cryobiology

authors： Orjuela-Palacio JM,Graiver N,Santos MV,Zaritzky NE

更新日期：2019-08-01 00:00:00