当前位置： SCI文献检索 > CRYOBIOLOGY期刊下所有文献 > Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants.

Studies on membrane permeability of zebrafish (Danio rerio) oocytes in the presence of different cryoprotectants.

Abstract：

:Investigation into fish oocyte membrane permeability is essential for developing successful protocols for their cryopreservation. The aim of the present work was to study the permeability of the zebrafish (Danio rerio) oocyte membrane to water and cryoprotectants before cryopreservation protocol design. The study was conducted on stage III and stage V zebrafish oocytes. Volumetric changes of stage III oocytes in different concentrations of sucrose were measured after 20 min exposure at 22 degrees C and the osmotically inactive volume of the oocytes (Vb) was determined using the Boyle-van't Hoff relationship. Volumetric changes of oocytes during exposure to different cryoprotectant solutions were also measured. Oocytes were exposed to 2 M dimethyl sulphoxide (DMSO), propylene glycol (PG), and methanol for 40 min at 22 degrees C. Stage III oocytes were also exposed to 2 M DMSO at 0 degrees C. Oocyte images were captured on an Olympus BX51 cryomicroscope using Linkham software for image recording. Scion Image was used for image analysis and diameter measurement. The experimental data were fitted to a two-parameter model using Berkeley Madonna 8.0.1 software. Hydraulic conductivity (L(p)) and solute (cryoprotectant) permeability (Ps) were estimated using the model. The osmotically inactive volume of stage III zebrafish oocytes was found to be 69.5%. The mean values+/-SE of Lp were found to be 0.169+/-0.02 and 0.196+/-0.01 microm/min/atm in the presence of DMSO and PG, respectively, at 22 degrees C, assuming an internal isosmotic value for the oocyte of 272 mOsm. The Ps values were 0.000948+/-0.00015 and 0.000933+/-0.00005 cm/min for DMSO and PG, respectively. It was also shown that the membrane permeability of stage III oocytes decreased significantly with temperature. No significant changes in cell volume during methanol treatment were observed. Fish oocyte membrane permeability parameters are reported here for the first time. The Lp and Ps values obtained for stage III zebrafish oocytes are generally lower than those obtained from successfully cryopreserved mammalian oocytes and higher than those obtained with fish embryos and sea urchin eggs. It was not possible to estimate membrane permeability parameters for stage V oocytes using the methods employed in this study because stage V oocytes experienced the separation of outer oolemma membrane from inner vitelline during exposure to cryoprotectants.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Zhang T,Isayeva A,Adams SL,Rawson DM

doi

10.1016/j.cryobiol.2005.02.007

subject

Has Abstract

pub_date

2005-06-01 00:00:00

pages

285-93

issue

3

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(05)00034-9

journal_volume

50

pub_type

杂志文章

相关文献

CRYOBIOLOGY文献大全
  • Strategies for exploration of freeze responsive gene expression: advances in vertebrate freeze tolerance.

    abstract：:Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2003.10.008

    authors： Storey KB

    更新日期：2004-04-01 00:00:00

  • Improvement of the post-thaw qualities of Okinawan native pig spermatozoa frozen in an extender supplemented with ascorbic acid 2-O-alpha-glucoside.

    abstract：:The technical establishment of boar sperm cryopreservation is indispensable for effective breeding of the scarce Okinawan native pig Agu. The objective of the present study was to determine whether ascorbic acid 2-O-alpha-glucoside (AA-2G), a stable ascorbate derivative, is capable of improving the quality of cryopres...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.05.002

    authors： Yoshimoto T,Nakamura S,Yamauchi S,Muto N,Nakada T,Ashizawa K,Tatemoto H

    更新日期：2008-08-01 00:00:00

  • The effects of preservation procedures on amniotic membrane's ability to serve as a substrate for cultivation of endothelial cells.

    abstract：:Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2011.08.003

    authors： Niknejad H,Deihim T,Solati-Hashjin M,Peirovi H

    更新日期：2011-12-01 00:00:00

  • Fc receptors and human T lymphocytes in frozen-thawed peripheral blood cells.

    abstract：:The present study was carried out to investigate the influence of cryopreservation on human T-cell subsets defined by their membrane receptors for Fc IgM (TM) and Fc IgG (TG) and by their membrane antigens. For this purpose isolated T cells, obtained by neuraminidase-treated sheep erythrocyte (E-N) rosetting, and enri...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(84)90080-4

    authors： Pusztai-Markos Z,Ebenfeld R,Scheiwe MW

    更新日期：1984-08-01 00:00:00

  • Comparison of three methods for human corneal cryopreservation that utilize dimethyl sulfoxide.

    abstract：:We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1999.2182

    authors： Bourne WM,Nelson LR,Hodge DO

    更新日期：1999-08-01 00:00:00

  • Cryoprotection of red blood cells by a 2,3-butanediol containing mainly the levo and dextro isomers.

    abstract：:A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(92)90036-2

    authors： Boutron P

    更新日期：1992-06-01 00:00:00

  • Non-toxic freezing media to retain the stem cell reserves in adipose tissues.

    abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.07.005

    authors： Shaik S,Wu X,Gimble JM,Devireddy R

    更新日期：2020-10-01 00:00:00

  • Development of a spermatogonia cryopreservation protocol for blue catfish, Ictalurus furcatus.

    abstract：:Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonial stem cells (SSCs) from blue catfish into channel catfish hosts has the potential to greatly increase gamete availab...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.10.010

    authors： Abualreesh M,Myers JN,Gurbatow J,Johnson A,Xing,Wang J,Li S,Coogan M,Vo K,El Husseini N,Dunham RA,Butts IAE

    更新日期：2020-12-01 00:00:00

  • Long-term (5 years) cryopreserved spermatogonia have high capacity to generate functional gametes via interspecies transplantation in salmonids.

    abstract：:Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.08.001

    authors： Lee S,Iwasaki Y,Yoshizaki G

    更新日期：2016-10-01 00:00:00

  • A new cryomacroscope device (Type III) for visualization of physical events in cryopreservation with applications to vitrification and synthetic ice modulators.

    abstract：:The objective of the current study is to develop a new cryomacroscope prototype for the study of vitrification in large-size specimens. The unique contribution in the current study is in developing a cryomacroscope setup as an add-on device to a commercial controlled-rate cooler and in demonstration of physical events...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2013.08.005

    authors： Rabin Y,Taylor MJ,Feig JS,Baicu S,Chen Z

    更新日期：2013-12-01 00:00:00

  • Vitrification of mature mouse oocytes in a 6 M Me2SO solution supplemented with antifreeze glycoproteins: the effect of temperature.

    abstract：:Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2098

    authors： O'Neil L,Paynter SJ,Fuller BJ,Shaw RW,DeVries AL

    更新日期：1998-08-01 00:00:00

  • Novel technical strategies to optimize cryopreservation of goat semen using cholesterol-loaded cyclodextrin.

    abstract：:Artificial insemination is well-established in dairy cattle, with sires housed in commercial studs for processing. In some species, however, sires located on-farm are used for artificial insemination by shipping their semen to an off-site laboratory for processing within 24 h of collection. To expedite semen transport...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.12.010

    authors： Salmon VM,Leclerc P,Bailey JL

    更新日期：2017-02-01 00:00:00

  • In vitro fertilization of ovine oocytes vitrified by solid surface vitrification at germinal vesicle stage.

    abstract：:Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2012.04.008

    authors： Moawad AR,Fisher P,Zhu J,Choi I,Polgar Z,Dinnyes A,Campbell KH

    更新日期：2012-10-01 00:00:00

  • MicroRNA regulation below zero: differential expression of miRNA-21 and miRNA-16 during freezing in wood frogs.

    abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.08.009

    authors： Biggar KK,Dubuc A,Storey K

    更新日期：2009-12-01 00:00:00

  • Application of the osmotic virial equation in cryobiology.

    abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.07.011

    authors： Prickett RC,Elliott JA,McGann LE

    更新日期：2010-02-01 00:00:00

  • Osmotic sensitivity of canine spermatozoa.

    abstract：:The objective of this study was to determine osmotic tolerance of canine spermatozoa. The study comprised three experiments: (1) spermatozoa suspended either in an egg yolk-citrate (EYC) extender or in Kenney skim milk extender were exposed to NaCl solutions ranging from 290 to 1500 mOsm; (2) spermatozoa suspended in ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/S0011-2240(02)00009-3

    authors： Songsasen N,Yu I,Murton S,Paccamonti DL,Eilts BE,Godke RA,Leibo SP

    更新日期：2002-02-01 00:00:00

  • Effects of different cryoprotectants on the viability and biological characteristics of porcine preadipocyte.

    abstract：:Effective techniques for the cryopreservation of porcine preadipocytes could increase the usefulness of these cells as a model in obesity studies. The objective of this study was to test the effects of the following cryoprotective agents (CPAs) on the cytotoxicity, post-thaw survival, proliferation and differentiation...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2006.06.007

    authors： Li Y,Lu RH,Luo GF,Pang WJ,Yang GS

    更新日期：2006-10-01 00:00:00

  • The death pathways in mussel larval cells after a freeze-thaw cycle.

    abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.05.009

    authors： Odintsova NA,Boroda AV,Maiorova MA,Yakovlev KV

    更新日期：2017-08-01 00:00:00

  • Improved recovery of post-thaw motility and vitality of human spermatozoa cryopreserved in the presence of dithiothreitol.

    abstract：:Semen was collected in the laboratory from nine healthy donors. The concentrations and the percentages of live and motile spermatozoa in all semen samples were within the normal range. Each sample was diluted with citrate-egg yolk-glycerol medium with and without 5 mM dithiothreitol (DTT). Samples were frozen in liqui...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(84)90052-x

    authors： Rao B,David G

    更新日期：1984-10-01 00:00:00

  • A novel strategy for conservation of European eel (Anguilla anguilla) genetic resources: Cryopreservation of ovarian stem cells.

    abstract：:The aim of this study was to develop short- and long-term preservation protocols for European eel ovarian stem cells (OSCs) through hypothermic storage and cryopreservation of ovarian fragments that will assist in current conservation programs of this critically endangered species. Firstly, a freezing procedure was de...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.03.009

    authors： Šćekić I,Marinović Z,Lujić J,Müller T,Kitanović N,Urbányi B,Horváth Á

    更新日期：2020-08-01 00:00:00

  • Introduction to the special issue: Thermodynamic aspects of cryobiology.

    abstract：:This brief paper introduces the subject of thermodynamics and the papers of the special issue on thermodynamic aspects of cryobiology. Thermodynamic terminology is defined for the non-specialist. ...

    journal_title：Cryobiology

    pub_type：

    doi：10.1016/j.cryobiol.2009.07.006

    authors： Elliott JA

    更新日期：2010-02-01 00:00:00

  • Effect of sulforaphane on apoptosis, reactive oxygen species and lipids peroxidation of human sperm during cryopreservation.

    abstract：:Sperm cryopreservation is a common procedure to preserve viable sperm for an indefinite period. This procedure has numerous detrimental effects on sperm function due to increased generation of reactive oxygen species (ROS). During cryopreservation, while ROS increases, antioxidant enzymes level decreases. It has been ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.11.012

    authors： Valipour J,Nashtaei MS,Khosravizadeh Z,Mahdavinezhad F,Nekoonam S,Esfandyari S,Amidi F

    更新日期：2020-11-26 00:00:00

  • Effect of cryopreservation on apple genetic resources at morphological, chromosomal, and molecular levels.

    abstract：:Shoot tips of three apple genotypes, namely, Malus pumila cv. M26, Gala, and Hokkaido No. 9, were successfully cryopreserved using a modified encapsulation-dehydration method. As a result, in addition to a high survival rate and regeneration rate, the capacity of shoots regenerated from cryopreserved samples to root w...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.2001.2339

    authors： Hao YJ,Liu QL,Deng XX

    更新日期：2001-08-01 00:00:00

  • Thermal stability properties of an antifreeze protein from the desert beetle Microdera punctipennis.

    abstract：:An insect antifreeze protein gene Mpafp698 was cloned by the RT-PCR approach from the desert beetle Microdera punctipennis. The gene was constructed and heterogeneously expressed in Escherichia coli as fusion proteins, His-MpAFP698, glutathione S-transferase (GST)-MpAFP698, and maltose-binding protein (MBP)-MpAFP698. ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.10.014

    authors： Qiu LM,Ma J,Wang J,Zhang FC,Wang Y

    更新日期：2010-04-01 00:00:00

  • Defining the optimal cryoprotectant and concentration for cryopreservation of limbal stem cells.

    abstract：:Limbal stem cell (LSC) deficiency causes progressive loss of vision but may be treated by transplant of autologous LSCs. Cryopreservation has the potential to indefinitely extend the lifespan of LSCs allowing re-transplant in case of graft failure. In this study, we aimed to identify the optimal cryoprotectant and cry...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.07.008

    authors： Osei-Bempong C,Ghareeb AE,Lako M,Figueiredo FC,Armitage WJ

    更新日期：2018-10-01 00:00:00

  • Osmotic parameters of red blood cells from umbilical cord blood.

    abstract：:The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.04.002

    authors： Zhurova M,McGann LE,Acker JP

    更新日期：2014-06-01 00:00:00

  • Cryopreservation of turkey semen: effect of breeding line and freezing method on post-thaw sperm quality, fertilization, and hatching.

    abstract：:Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.04.003

    authors： Long JA,Purdy PH,Zuidberg K,Hiemstra SJ,Velleman SG,Woelders H

    更新日期：2014-06-01 00:00:00

  • Cryopreservation of amniotic fluid-derived stem cells using natural cryoprotectants and low concentrations of dimethylsulfoxide.

    abstract：:Amniotic fluid-derived stem cells (AFSCs) are a potential cell source for therapeutic applications. They can be easily mass produced, cryopreserved and shipped to clinics for immediate use. However, one major obstacle to the manufacturing of clinical grade stem cells is the need for current good manufacturing practice...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2011.02.003

    authors： Seo JM,Sohn MY,Suh JS,Atala A,Yoo JJ,Shon YH

    更新日期：2011-06-01 00:00:00

  • Sperm cryopreservation in guppies and black mollies--a generalized freezing protocol for livebearers in Poeciliidae.

    abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2009.09.011

    authors： Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

    更新日期：2009-12-01 00:00:00

  • Determination of cryothermal injury thresholds in tissues impacted by cardiac cryoablation.

    abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2017.01.002

    authors： Goff RP,Quallich SG,Buechler RA,Bischof JC,Iaizzo PA

    更新日期：2017-04-01 00:00:00