Oscillations of trypsinogen activation.

abstract：:Few tissues or organisms can survive the removal of nearly all their intra and extracellular water. These few have developed specialized adaptations to protect their cellular components from the damage caused by desiccation and rehydration. One mechanism, common to almost all such organisms, is the accumulation of dis...

journal_title：Cryobiology

authors： Oliver AE,Leprince O,Wolkers WF,Hincha DK,Heyer AG,Crowe JH

更新日期：2001-09-01 00:00:00

abstract：:The objective of this study is to examine the effects of the most widely used high-molecular-weight cryoprotectants on the coagulation system. Dextran, hydryoxyethyl starch (HES), polyvinyl pyrrolidone (PVP), polyethylene glycol (PEG), and albumin were added at different concentrations in the range between 0.01-1% (w/...

journal_title：Cryobiology

authors： Bakaltcheva I,Ganong JP,Holtz BL,Peat RA,Reid T

更新日期：2000-06-01 00:00:00

abstract：:The present study was carried out to investigate the influence of cryopreservation on human T-cell subsets defined by their membrane receptors for Fc IgM (TM) and Fc IgG (TG) and by their membrane antigens. For this purpose isolated T cells, obtained by neuraminidase-treated sheep erythrocyte (E-N) rosetting, and enri...

journal_title：Cryobiology

authors： Pusztai-Markos Z,Ebenfeld R,Scheiwe MW

更新日期：1984-08-01 00:00:00

abstract：:Cryopreservation procedures cause osmotic stress to spermatozoa following cryoinjury and reduce their content of osmolytes. Conventional method for cryoprotectant loading and dilution on canine semen freezing which could be categorized in single step protocol, makes decreasing in sperm performance such as motility, mo...

journal_title：Cryobiology

authors： Setyawan EM,Kim MJ,Oh HJ,Kim GA,Jo YK,Lee SH,Choi YB,Lee BC

更新日期：2015-10-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:Trehalose, a non-reducing glucose disaccharide found at high concentrations in many species of anhydrobiotic organisms, shows significant promise in protecting cellular viability and structural integrity during freezing and desiccation. As mammalian cell membranes are impermeable to trehalose, extensive efforts have b...

journal_title：Cryobiology

authors： Holovati JL,Acker JP

更新日期：2007-10-01 00:00:00

abstract：:In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...

journal_title：Cryobiology

authors： Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

更新日期：2011-04-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...

journal_title：Cryobiology

authors： Benson JD

更新日期：2012-04-01 00:00:00

abstract：:Previous reports established that butylated hydroxy toluene (BHT) minimized cold-induced membrane rupture in sperm from several species. No data regarding the specificity of its effect is available. In this study 25 BHT analogs were tested for their effect on bovine sperm membrane stability. Fourteen were membrane lyt...

journal_title：Cryobiology

authors： Graham JK,Hammerstedt RH

更新日期：1992-02-01 00:00:00

abstract：:This study was conducted to evaluate the effects of developmental stage of in vitro produced (IVP) ovine embryos and the type of vitrification procedure used on embryo cryotolerance. The IVP embryos were vitrified at five different developmental stages: 4-, 8- and 16-cell, morula, and blastocyst. For each stage, half ...

journal_title：Cryobiology

authors： Shirazi A,Soleimani M,Karimi M,Nazari H,Ahmadi E,Heidari B

更新日期：2010-04-01 00:00:00

abstract：:(1) Tyrosine and tryptophan metabolism in brain and peripheral tissues were studied in hypothermic hibernating and normothermic nonhibernating 13-lined ground squirrels (Spermophilus tridecemlineatus). (2) In the hypothermic hibernating state, there were significant elevations of brain stem tyrosine, norepinephrine, a...

journal_title：Cryobiology

authors： Weekley BL,Harlow HJ

更新日期：1987-12-01 00:00:00

abstract：:The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...

journal_title：Cryobiology

authors： Murakami M,Otoi T,Sumantri C,Suzuki T

更新日期：1998-05-01 00:00:00

abstract：:This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...

journal_title：Cryobiology

authors： Hoffmann NE,Coad JE,Huot CS,Swanlund DJ,Bischof JC

更新日期：2001-02-01 00:00:00

abstract：:Two experiments were conducted to investigate the relationship between survival rates and ultrastructural appearance, using the freeze-replica technique, of bovine blastocysts after vitrification. In experiment 1, blastocysts obtained from in vitro-matured and in vitro-fertilized (IVM-IVF) bovine oocytes were either e...

journal_title：Cryobiology

authors： Kuwayama M,Fujikawa S,Nagai T

更新日期：1994-10-01 00:00:00

abstract：:Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...

journal_title：Cryobiology

authors： Wolkers WF,Walker NJ,Tablin F,Crowe JH

更新日期：2001-03-01 00:00:00

abstract：:This study investigated factors important to the development of the liquid nitrogen (LN) vapor sperm cryopreservation technique in farmed greenlip abalone Haliotis laevigata, including (1) cryoprotectant agent (CPA) toxicity; (2) cooling temperature (height above LN surface); (3) thawing temperature; (4) sperm to egg ...

journal_title：Cryobiology

authors： Liu Y,Xu T,Robinson N,Qin J,Li X

更新日期：2014-04-01 00:00:00

abstract：:We analyzed cell viability, caspase activity, plasma membrane alterations and cell ultrastructure morphology to estimate the morphological and biochemical alterations that occur in bivalve molluscan cell cultures during cryopreservation. The use of 5% dymethyl sulfoxide as a cryoprotectant resulted in greater cell sur...

journal_title：Cryobiology

authors： Odintsova NA,Boroda AV,Maiorova MA,Yakovlev KV

更新日期：2017-08-01 00:00:00

abstract：:Morulae and unhatched blastocysts from Large White hyperprolific (LWh) and Meishan (MS) gilts were selected to test an ultrarapid open pulled straw (OPS) vitrification method with two media. The viability of vitrified/warmed embryos was estimated by the percentage of embryos that developed to the hatched blastocyst st...

journal_title：Cryobiology

authors： Berthelot F,Martinat-Botté F,Locatelli A,Perreau C,Terqui M

更新日期：2000-09-01 00:00:00

abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...

journal_title：Cryobiology

authors： Zhang T,Rawson DM,Tosti L,Carnevali O

更新日期：2008-04-01 00:00:00

abstract：:A cryomicroscope is described which provides the possibility of quantifying the volume loss of cells during freezing, detection of intracellular ice formation during cooling and warming, as well as the determination of viability as function of (constant) cooling rates. The basic mechanisms occurring in cryopreservatio...

journal_title：Cryobiology

authors： Scheiwe MW,Körber C

更新日期：1984-02-01 00:00:00

abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

journal_title：Cryobiology

authors： Stott SL,Karlsson JOM

更新日期：2009-02-01 00:00:00

abstract：:The osteogenic capacity of human umbilical cord blood derived mesenchymal stem cells (UCB-MSCs) has been demonstrated both in vitro and in vivo. Therefore, cell labeling and storage are becoming necessary for researching the potential therapeutic use of UCB-MSCs for bone tissue engineering. The aim of this study was t...

journal_title：Cryobiology

authors： Liu G,Ye X,Zhu Y,Li Y,Sun J,Cui L,Cao Y

更新日期：2011-10-01 00:00:00

abstract：:Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...

journal_title：Cryobiology

authors： Vogiatzi C,Rosenqvist E,Grout BWW

更新日期：2018-05-06 00:00:00

abstract：:Amniotic membrane (AM) has been used as a scaffold for the ex vivo expansion of different types of cells and a cell delivery matrix in regenerative medicine. Since the preservation procedures can influence the AM properties for experimental and clinical purposes, this study was established to investigate the feasibili...

journal_title：Cryobiology

authors： Niknejad H,Deihim T,Solati-Hashjin M,Peirovi H

更新日期：2011-12-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The present study examined the developmental capacity and cryotolerance of cultured bovine embryos in defined media (synthetic oviduct fluid, SOF) supplemented with insulin-like growth factor I (IGF-I) and leukemia inhibitor factor (LIF). The objectives of the present study were: (1) to examine the effects IGF-I and L...

journal_title：Cryobiology

authors： Kocyigit A,Cevik M

更新日期：2015-08-01 00:00:00

abstract：:A recent study from our laboratory indicated additional tissue injury during rewarming of a cooled rabbit leg. Oxygen-derived free radicals were believed to play a role in such "rewarming injury." Since free radicals may attack membrane phospholipids, we analyzed the phospholipid composition in the leg tissue during c...

journal_title：Cryobiology

authors： Das DK,Iyengar J,Jones RM,Lu D,Maity S

更新日期：1991-04-01 00:00:00

abstract：:In order to preserve genetic resources of chum salmon, Oncorhynchus keta, optimum conditions for cryopreservation of isolated blastomeres were investigated. Survival rates under various conditions were compared: the nature and the concentration of cryoprotectants before and after freezing, the seeding temperature, and...

journal_title：Cryobiology

authors： Kusuda S,Teranishi T,Koide N

更新日期：2002-08-01 00:00:00

abstract：:Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyo...

journal_title：Cryobiology

authors： Lavenburg VM,Liao YT,Salvador A,Hsu AL,Harden LA,Wu VCH

更新日期：2020-10-01 00:00:00