Altered tyrosine and tryptophan metabolism during hypothermic hibernation in the 13-lined ground squirrel (Spermophilus tridecemlineatus).

abstract：:The effects of the presence or absence of cumulus cells and equilibration times (1 and 4 min) with cryoprotectant, and Taxol treatment before vitrification (0.5-5.0 microM) on development of in vitro matured porcine oocytes after vitrification were examined. Ethylene glycol (30%) and sucrose (0.5M) was used as a vitri...

journal_title：Cryobiology

authors： Fujihira T,Nagai H,Fukui Y

更新日期：2005-12-01 00:00:00

abstract：:The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated ...

journal_title：Cryobiology

authors： Puhlev I,Guo N,Brown DR,Levine F

更新日期：2001-05-01 00:00:00

abstract：:Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...

journal_title：Cryobiology

authors： Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

更新日期：1997-11-01 00:00:00

abstract：:The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...

journal_title：Cryobiology

authors： Allen ED,Gau TC,Natale RB

更新日期：1984-04-01 00:00:00

abstract：:A total of 498 porcine embryos at various stages of development collected from superovulated gilts was used to investigate cryopreservation. First, blastocysts (BL), expanded blastocysts (ExB), and hatched blastocysts (HB) were used to determine the effect of exposure to concentrated solutions of ethylene glycol as cr...

journal_title：Cryobiology

authors： Kobayashi S,Takei M,Kano M,Tomita M,Leibo SP

更新日期：1998-02-01 00:00:00

abstract：:Two experiments were conducted to investigate the relationship between survival rates and ultrastructural appearance, using the freeze-replica technique, of bovine blastocysts after vitrification. In experiment 1, blastocysts obtained from in vitro-matured and in vitro-fertilized (IVM-IVF) bovine oocytes were either e...

journal_title：Cryobiology

authors： Kuwayama M,Fujikawa S,Nagai T

更新日期：1994-10-01 00:00:00

abstract：:Cryoablation is a minimally invasive cancer treatment. In this study, the effects of cryoablation on normal rabbit bone were evaluated using imaging and histopathological examinations. Cryoablation was performed using a Cryo-Hit (Galil Medical, Yokneam, Israel). Under anesthesia, one cryoablation needle was inserted a...

journal_title：Cryobiology

authors： Yoshimoto Y,Azuma K,Miya A,Makino E,Nakamoto H,Abe N,Kaburagi M,Ueda H,Kuroda K,Tsuka T,Sugiyama A,Imagawa T,Murahata Y,Itoh N,Osaki T,Shimizu T,Okamoto Y

更新日期：2014-10-01 00:00:00

abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

journal_title：Cryobiology

authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

更新日期：2007-10-01 00:00:00

abstract：:A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...

journal_title：Cryobiology

authors： Stott SL,Karlsson JOM

更新日期：2009-02-01 00:00:00

abstract：:Our objective was to study the effect of the concentration of ethylene glycol (EG) and dimethyl sulfoxide (Me2SO) during vitrification on the development of porcine blastocysts. Vitrification was performed with 0.4 M sucrose and either a Me2SO and EG mixture (15%, 16% and 17% v/v of each) or EG alone (40% v/v), using ...

journal_title：Cryobiology

authors： Cuello C,Sanchez-Osorio J,Almiñana C,Gil MA,Perals ML,Lucas X,Roca J,Vazquez JM,Martinez EA

更新日期：2008-06-01 00:00:00

abstract：:The utility of microalgal biomass and bioproducts depends on long-term maintenance of certain physiological or biochemical features of the species. While unique characteristics may not be durably maintained with general subculture, cryopreservation methods better prevent alterations from desired characteristics. Post-...

journal_title：Cryobiology

authors： Zheng L,Lu Z,Zhang Q,Li T,Song L

更新日期：2018-04-01 00:00:00

abstract：:In the determination of the solid-liquid phase equilibria in the aqueous mixtures of dimethyl sulfoxide (Me2SO) and ethylene glycol (EG) one often encounters the problem of equilibrium crystallization. In the present report the above aqueous solutions are equilibrated for crystallization in a dielectric cell during wh...

journal_title：Cryobiology

authors： Murthy SS

更新日期：1998-03-01 00:00:00

abstract：:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...

journal_title：Cryobiology

authors： Armitage WJ,Juss BK

更新日期：2003-04-01 00:00:00

abstract：:Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...

journal_title：Cryobiology

authors： Duker GD,Olsson SO,Hecht NH,Senturia JB,Johansson BW

更新日期：1983-08-01 00:00:00

abstract：:The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...

journal_title：Cryobiology

authors： Zhurova M,McGann LE,Acker JP

更新日期：2014-06-01 00:00:00

abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...

journal_title：Cryobiology

authors： Lautner LJ,Freed DH,Nagendran J,Acker JP

更新日期：2020-06-01 00:00:00

abstract：:Recently, a non-invasive delipation (lipid removal) method combined with ultrarapid vitrification has been used successfully for in vitro produced (IVP) porcine embryos. In the present study, this method was combined with parthenogenesis and a recent form of somatic cell nuclear transfer (SCNT) - handmade cloning (HMC...

journal_title：Cryobiology

authors： Du Y,Zhang Y,Li J,Kragh PM,Kuwayama M,Ieda S,Zhang X,Schmidt M,Bøgh IB,Purup S,Pedersen AM,Villemoes K,Yang H,Bolund L,Vajta G

更新日期：2007-04-01 00:00:00

abstract：:This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...

journal_title：Cryobiology

authors： Seifi-Jamadi A,Ahmad E,Ansari M,Kohram H

更新日期：2017-04-01 00:00:00

abstract：:Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0±2.0°C and 8.0±2....

journal_title：Cryobiology

authors： Paes Mdo C,da Silva RC,do Nascimento NF,Valentin FN,Senhorini JA,Nakaghi LS

更新日期：2014-12-01 00:00:00

abstract：:Ice-nucleating active (INA) bacteria were isolated from the gut of field-collected freeze-tolerant wood frogs (Rana sylvatica) collected in winter. Thirteen strains of Pseudomonas fluorescens, four strains of Pseudomonas putida, and two strains of Enterobacter agglomerans had ice-nucleating activity. Each of the INA p...

journal_title：Cryobiology

authors： Lee MR,Lee RE Jr,Strong-Gunderson JM,Minges SR

更新日期：1995-08-01 00:00:00

abstract：:The multisolute osmotic virial equation is the only multisolute thermodynamic solution theory that has been derived from first principles and can make predictions of multisolute solution behaviour in the absence of multisolute solution data. Other solution theories either (i) include simplifying assumptions that do no...

journal_title：Cryobiology

authors： Prickett RC,Elliott JA,McGann LE

更新日期：2010-02-01 00:00:00

abstract：:Hypothermia is used in the clinic for protection of organs such as the brain against ischemic injury during aortic/complex congenital cardiac surgery or post-resuscitation encephalopathy. The principal mechanism of hypothermic protection is suppression of metabolism, however, the pleiotropic effects of cooling are inc...

journal_title：Cryobiology

authors： Oda T,Shimizu K,Yamaguchi A,Satoh K,Matsumoto K

更新日期：2012-10-01 00:00:00

abstract：:The freeze tolerance and accumulation of cryoprotectants was investigated in three geographically different populations of the enchytraeid Enchytraeus albidus (Oligochaeta). E. albidus is widely distributed from the high Arctic to temperate Western Europe. Our results show that E. albidus is freeze tolerant, with free...

journal_title：Cryobiology

authors： Slotsbo S,Maraldo K,Malmendal A,Nielsen NC,Holmstrup M

更新日期：2008-12-01 00:00:00

abstract：:The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...

journal_title：Cryobiology

authors： Acker JP,McGann LE

更新日期：2000-02-01 00:00:00

abstract：:The soil microalgae of the genus Heterococcus are found in cold environments and have been reported for the terrestrial ecosystems of several Sub-Antarctic and Antarctic Islands. This study focused on resistance of Heterococcus sp. to sub-zero temperature. Heterococcus sp. was isolated from soil samples from James Ros...

journal_title：Cryobiology

authors： Orekhova A,Barták M,Hájek J

更新日期：2018-12-01 00:00:00

abstract：:Successful fish embryo cryopreservation is still far from being achieved. Vitrification is considered the most promising option. Many factors are involved in the success of the process. The choice of a proper vitrification solution, the enzymatic permeabilization of embryos to increase cryoprotectant permeability, the...

journal_title：Cryobiology

authors： Robles V,Cabrita E,Real M,Alvarez R,Herráez MP

更新日期：2003-08-01 00:00:00

abstract：:In this study, we evaluated various parameters of sperm cryopreservation in two livebearers, guppies (Poecilia reticulata) and black mollies (P. latipinna). Our results suggested a common freezing protocol for the guppies and mollies: suspend sperm in Hanks' balanced salt solution (HBSS) at 300 mOsm/kg, use 14% glycer...

journal_title：Cryobiology

authors： Huang C,Sun C,Su X,Zhao X,Miao M,Liu Y,Dong Q

更新日期：2009-12-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00