Freezing monolayers of cells without gap junctions.

Abstract:

:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread of ice between neighbouring cells. MDCK Type II cells do not form gap junctions in monolayer culture. When frozen at rates of 0.2 to 10 degrees C/min, monolayers in 10% (v/v) propane-1,2-diol or dimethyl sulphoxide showed little influence of cooling rate on survival. This suggested that, in the absence of gap junctions, cells in monolayers did not display enhanced susceptibility to intracellular freezing. In contrast, however, monolayers frozen in glycerol showed a marked increase in cell damage when cooled at rates higher than 0.5 degrees C/min. This does not necessarily counter the suggestion that lack of gap junctions mitigates intracellular freezing as there is evidence that glycerol may itself promote intracellular freezing.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Armitage WJ,Juss BK

doi

10.1016/s0011-2240(03)00017-8

subject

Has Abstract

pub_date

2003-04-01 00:00:00

pages

194-6

issue

2

eissn

0011-2240

issn

1090-2392

pii

S0011224003000178

journal_volume

46

pub_type

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