Osteogenic differentiation of GFP-labeled human umbilical cord blood derived mesenchymal stem cells after cryopreservation.

abstract：:Natural freeze tolerance depends on numerous biochemical adaptations that address the multiple stresses imposed on cells by freezing and preserves viability by suppressing energy-expensive cell functions in the frozen state. We hypothesized that microRNAs, small non-coding RNA transcripts that bind to mRNA, could act ...

journal_title：Cryobiology

authors： Biggar KK,Dubuc A,Storey K

更新日期：2009-12-01 00:00:00

abstract：:Cryopreservation is the only established method for long-term preservation of cells and cellular material. This technique involves preservation of cells and cellular components in the presence of cryoprotective agents (CPAs) at liquid nitrogen temperatures (-196 °C). The organic solvent dimethyl sulfoxide (Me2SO) is o...

journal_title：Cryobiology

authors： Solocinski J,Osgood Q,Wang M,Connolly A,Menze MA,Chakraborty N

更新日期：2017-04-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00

abstract：:Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...

journal_title：Cryobiology

authors： Baudot A,Cacela C,Duarte ML,Fausto R

更新日期：2002-04-01 00:00:00

abstract：:The effect of cryopreservation on ciliary activity of human nasal respiratory epithelium was evaluated. Samples were cryopreserved in a solution containing nutrient medium, 10% fetal calf serum, and two different concentrations (10 or 20%) of dimethyl sulfoxide and stored in liquid nitrogen at -196 degrees C for 2 wee...

journal_title：Cryobiology

authors： Di Benedetto G,Gill J,Lopez-Vidriero MT,Clarke SW

更新日期：1989-08-01 00:00:00

abstract：:Antifreeze proteins (AFP) play an important role in cellular survival at sub-zero temperatures. This study assessed the effect of AFP type I or III in semen extender (TRIS-egg yolk) for ram sperm cryopreservation. Pooled semen of four rams were allocated into five treatments: Control (CONT, without AFP); AFP Type I [0...

journal_title：Cryobiology

authors： Correia LFL,Espírito-Santo CG,Braga RF,Carvalho-de-Paula CJ,da Silva AA,Brandão FZ,Freitas VJF,Ungerfeld R,Souza-Fabjan JMG

更新日期：2020-11-10 00:00:00

abstract：:Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure t...

journal_title：Cryobiology

authors： Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

更新日期：1983-02-01 00:00:00

abstract：:Oocyte vitrification has extensively been applied in the field of embryo engineering and in the preservation of genetic resources of fine livestock. Following our previous work in oocyte vitrification and the level change of DNA methylation, here we further explored the dynamic change of three active demethylation pro...

journal_title：Cryobiology

authors： Fu L,Chang H,Wang Z,Xie X,Chen H,Lei Z,Zhang Y,Quan F

更新日期：2019-10-01 00:00:00

abstract：:Lactate dehydrogenase (LDH) was chemically ethyl-acetimidated (EA-), dimethyladipimidated (DMA-), carbamylated, acetylated, acetoacetylated, or succinylated in order to alter the ionic charges on the epsilon-amino group of lysine residues. Acetylation, acetoacetylation, and succinylation, which change the positive cha...

journal_title：Cryobiology

authors： Seguro K,Tamiya T,Tsuchiya T,Matsumoto JJ

更新日期：1989-04-01 00:00:00

abstract：:There is increasing interest in the possibility of treating diseased or damaged areas of synovial joint surfaces by grafts of healthy allogeneic cartilage. Such grafts could be obtained from cadaver tissue donors or in the future they might be manufactured by 'tissue engineering' methods. Cartilage is an avascular tis...

journal_title：Cryobiology

authors： Pegg DE,Wusteman MC,Wang L

更新日期：2006-06-01 00:00:00

abstract：:In this work, the protective capacity of galacto-oligosaccharides in the preservation of Lactobacillus delbrueckii subsp. bulgaricus CIDCA 333 was evaluated. Lactobacillus bulgaricus was freeze-dried or dried over silica gel in the presence of three commercial products containing galacto-oligosaccharides. The freeze-d...

journal_title：Cryobiology

authors： Tymczyszyn EE,Gerbino E,Illanes A,Gómez-Zavaglia A

更新日期：2011-04-01 00:00:00

abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...

journal_title：Cryobiology

authors： De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

更新日期：1998-09-01 00:00:00

abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...

journal_title：Cryobiology

authors： Izamis ML,Perk S,Calhoun C,Uygun K,Yarmush ML,Berthiaume F

更新日期：2015-10-01 00:00:00

abstract：:Previous reports established that butylated hydroxy toluene (BHT) minimized cold-induced membrane rupture in sperm from several species. No data regarding the specificity of its effect is available. In this study 25 BHT analogs were tested for their effect on bovine sperm membrane stability. Fourteen were membrane lyt...

journal_title：Cryobiology

authors： Graham JK,Hammerstedt RH

更新日期：1992-02-01 00:00:00

abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

journal_title：Cryobiology

authors： Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

更新日期：2009-04-01 00:00:00

abstract：:We have previously shown that cold-induced injury to hepatocytes and liver endothelial cells occurs predominantly via an iron-dependent pathway. However, other groups have reported evidences suggesting that Ca(2+) ions could be involved in the process of cold-induced injury of liver cells. We here assessed the relativ...

journal_title：Cryobiology

authors： Knoop S,de Groot H,Rauen U

更新日期：2008-04-01 00:00:00

abstract：:Loading with monosaccharide can improve the quality of human red blood cells (hRBCs) frozen with polymer. But in vivo life span of hRBCs frozen with polymer and sugar is not determined. In this study, following incubation with glucose, mouse red blood cells (mRBCs) were frozen in liquid nitrogen for 24h using dextran ...

journal_title：Cryobiology

authors： Quan GB,Liu MX,Han Y,Fang L,Du W,Wang JX

更新日期：2010-08-01 00:00:00

abstract：:Dynamic mechanical analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analy...

journal_title：Cryobiology

authors： Gearing J,Malik KP,Matejtschuk P

更新日期：2010-08-01 00:00:00

abstract：:The susceptibility of in vitro matured (IVM) porcine oocytes to be fertilized in vitro after vitrification was investigated. IVM oocytes were cryopreserved by solid surface vitrification (SSV) or treated with cryoprotectants (toxicity control, TC). Control oocytes were not treated or vitrified. Live oocytes in the thr...

journal_title：Cryobiology

authors： Somfai T,Ozawa M,Noguchi J,Kaneko H,Kuriani Karja NW,Farhudin M,Dinnyés A,Nagai T,Kikuchi K

更新日期：2007-10-01 00:00:00

abstract：:Previous studies on cane toad (Bufo marinus; Bufonidae; Anura) sperm cryopreservation were extended to compare the effects of cryopreservation in established sucrose (non-ionic) diluents with cryopreservation in ionic diluents containing amphibian Ringer solutions (with and without egg-yolk). In addition, methanol was...

journal_title：Cryobiology

authors： Browne RK,Mahony M,Clulow J

更新日期：2002-06-01 00:00:00

abstract：:The tetra-anionic form of ATP (ATP4-) is known to induce monovalent and divalent ion fluxes in cells that express purinergic P2X7 receptors and with sustained application of ATP it has been shown that dyes as large as 831 Da can permeate the cell membrane. The current study explores the kinetics of loading alpha,alpha...

journal_title：Cryobiology

authors： Elliott GD,Liu XH,Cusick JL,Menze M,Vincent J,Witt T,Hand S,Toner M

更新日期：2006-02-01 00:00:00

abstract：:Human mesenchymal stem cell (hMSC) research has grown exponentially in the last decade. The ability to process and preserve these cells is vital to their use in stem cell therapy. As such, understanding the complex, molecular-based stress responses associated with biopreservation is necessary to improve outcomes and m...

journal_title：Cryobiology

authors： Corwin WL,Baust JM,Baust JG,Van Buskirk RG

更新日期：2014-04-01 00:00:00

abstract：:Renal epithelial cells from donor kidneys are susceptible to hypothermic preservation injury, which is attenuated when they over express the cytoskeletal linker protein ezrin. This study was designed to characterize the mechanisms of this protection. Renal epithelial cell lines were created from LLC-PK1 cells, which e...

journal_title：Cryobiology

authors： Tian T,Lindell SL,Lam M,Mangino MJ

更新日期：2012-08-01 00:00:00

abstract：:The [K+]/[Na+] ratio of rabbit renal cortical slices was used to examine, at 25 degrees C, the effects on viability of three cryoprotectant agents (CPA) (dimethyl sulfoxide (Me2SO), ethylene glycol, and glycerol) in combination with three vehicle solutions (Krebs-Henseleit (K-H), solution A, and RPS-2). Viability asse...

journal_title：Cryobiology

authors： Clark P,Fahy GM,Karow AM Jr

更新日期：1984-06-01 00:00:00

abstract：:Despite widespread clinical use of cryoablation, there remain questions regarding dosing and treatment times which may affect efficacy and collateral injury. Dosing and treatment times are directly related to the degree of cooling necessary for effective lesion formation. Human and swine atrial, ventricular, and lung ...

journal_title：Cryobiology

authors： Goff RP,Quallich SG,Buechler RA,Bischof JC,Iaizzo PA

更新日期：2017-04-01 00:00:00

abstract：:Human platelets were frozen in 0.5 M glycerol, 0.5 M glycerol + 3% Dextran T40, or 0.5 M glycerol + 5% mannitol. The recovery of active transport of 5-hydroxytryptamine (5-HT) and the hypotonic stress response after freezing were dependent on the rate of cooling: the optimum range of rates was between 12 and 23 degree...

journal_title：Cryobiology

authors： Brodthagen UA,Armitage WJ,Parmar N

更新日期：1985-02-01 00:00:00

abstract：:Antarctic basidiomycetous yeast Mrakia blollopis SK-4 has unique fermentability for various sugars under a low temperature condition. Hence, this yeast was used for ethanol fermentation from glucose and also for direct ethanol fermentation (DEF) from cellulosic biomass without/with Tween 80 at 10°C. Maximally, 48.2 g/...

journal_title：Cryobiology

authors： Tsuji M,Goshima T,Matsushika A,Kudoh S,Hoshino T

更新日期：2013-10-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...

journal_title：Cryobiology

authors： Boutron P

更新日期：1992-06-01 00:00:00

abstract：:The response of cells to freezing depends critically on the presence of an intact cell membrane. During rapid cooling, the cell plasma membrane may no longer be an effective barrier to ice propagation and can be breached by extracellular ice resulting in the nucleation of the supercooled cytoplasm. In tissues, the for...

journal_title：Cryobiology

authors： Acker JP,McGann LE

更新日期：2000-02-01 00:00:00