Abstract:
:Esophageal cancer is common in China. There is a lack of treatment strategies for metastatic esophageal cancer (MEC) after radical surgery on the primary tumor. Cryoablation is an attractive option because tumor necrosis can be safely induced in a minimally invasive manner. This study assessed its therapeutic effect in MEC after failure of radical surgery. One hundred and forty patients met the inclusion criteria from May, 2003 to March, 2011. Comprehensive cryotherapy of multiple metastases was performed on 105 patients; 35 received chemotherapy. No severe complications occurred during or after cryoablation. Overall survival (OS) was assessed according to therapeutic protocol, pathologic type, treatment timing and number of procedures. The OS of patients who received comprehensive cryoablation (44 ± 20 months) was significantly longer than that of those who underwent chemotherapy (23 ± 24 months; P = 0.0006). In the cryotherapy group, the OS for squamous cell carcinoma (45 ± 19 months) was longer than that for adenocarcinoma (33 ± 18 months; P = 0.0435); the OS for timely cryoablation (46 ± 19 months) was longer than that for delayed cryoablation (33 ± 20 months; P = 0.0193); the OS for multiple cryoablation (50 ± 17 months) was longer than that for single cryoablation (37 ± 20 months; P = 0.0172); and the OS for cryo-immunotherapy (56 ± 17 months) was longer than that for cryoablation alone (39 ± 19 months; P = 0.0011). Thus, comprehensive cryotherapy may have advantages over chemotherapy in the treatment of MEC and, in patients with squamous cell carcinoma, supplementary immunotherapy and timely and multiple cryoablation may be associated with a better prognosis.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Jiongyuan X,Lizhi N,Feng M,Shupeng L,Yin L,Mengtian L,Jianying Z,Fei Y,Jibing C,Jialiang L,Kecheng Xsubject
Has Abstractpub_date
2013-12-01 00:00:00pages
363-8issue
3eissn
0011-2240issn
1090-2392pii
S0011-2240(13)00391-Xjournal_volume
67pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::Winter survival for many cold-blooded species involves freeze tolerance, the capacity to endure the freezing of a high percentage of total body water as extracellular ice. The wood frog (Rana sylvatica) is the primary model animal used for studies of vertebrate freeze tolerance and current studies in my lab are focuse...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2003.10.008
更新日期:2004-04-01 00:00:00
abstract::A high-speed video cryomicroscopy system was developed, and used to observe the process of intracellular ice formation (IIF) during rapid freezing (130 degrees C/min) of bovine pulmonary artery endothelial cells adherent to glass substrates, or in suspension. Adherent cells were micropatterned, constraining cell attac...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.11.003
更新日期:2009-02-01 00:00:00
abstract::Uncontrolled hemorrhage is the primary cause of death in both blunt and penetrating liver trauma. Cryohemostasis was attempted in the past for elective liver surgery but did not gain popularity. During past decades, cryoequipment was refined and successfully used for tumor ablation. The purpose of the present study wa...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2000.2239
更新日期:2000-05-01 00:00:00
abstract::Human monocytes purified from peripheral blood by counterflow centrifugal elutriation were cryopreserved in a vitreous state at 1 atm pressure. The vitrification solution was Hanks' balanced salt solution (HBSS) containing (w/v) 20.5% Me2SO, 15.5% acetamide, 10% propylene glycol, and 6% polyethylene glycol. Fifteen mi...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(86)90001-5
更新日期:1986-04-01 00:00:00
abstract::This study was performed to evaluate the effectiveness of quercetin as a non-enzymatic antioxidant in combination with glycerol or Dimethylacetamide (DMA), on freezability of goat semen. Ejaculates from four healthy mature Mahabadi goats were collected using an artificial vagina. After primary processing, semen was po...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2017.03.002
更新日期:2017-04-01 00:00:00
abstract::Trehalose, a non-reducing glucose disaccharide found at high concentrations in many species of anhydrobiotic organisms, shows significant promise in protecting cellular viability and structural integrity during freezing and desiccation. As mammalian cell membranes are impermeable to trehalose, extensive efforts have b...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2007.06.003
更新日期:2007-10-01 00:00:00
abstract::1,3-Butanediol and 2,3-butanediol have been used in buffered solutions with 20, 30, or 35% (w/w) alcohol to cool erythrocytes to -196 degrees C at different cooling rates between 1 to 3500 degrees C/min, followed by slow or rapid rewarming. 1,3-butanediol shows the same shapes of red blood cell survival curves as 1,2-...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90019-3
更新日期:1988-02-01 00:00:00
abstract::Recently, a mathematical model able to describe the non-perfect osmotic behavior of cells during cryopreservation was proposed. The model improves the two-parameter formalism typically adopted in cryopreservation literature by allowing the transmembrane permeation of ions/salt, through the temporary opening of mechano...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.11.013
更新日期:2020-11-27 00:00:00
abstract::Cryopreservation is a technique that has been extensively used for storage of multipotent mesenchymal stromal cells (MSCs) in regenerative medicine. Therefore, improving current cryopreservation procedures in terms of increasing cell viability and functionality is important. In this study, we optimized the cryopreserv...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2015.10.145
更新日期:2015-12-01 00:00:00
abstract::The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.03.006
更新日期:2006-08-01 00:00:00
abstract::Cryopreservation in a vitrified state has vast potential for long-term storage of tissues and organs that may be damaged by ice formation. However, the toxicity imparted by the high concentration of cryoprotectants (CPAs) required to vitrify these specimens remains a hurdle. To address this challenge, we previously de...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2020.10.017
更新日期:2020-11-04 00:00:00
abstract::Low temperature studies with winter-dormant buds are severely limited by the lack of a rapid,non-destructive assay for their viability. Investigations involving the winter harvest of ecodormant buds of woody subjects, including cryopreservation, are restricted if viability cannot be assessed until dormancy is broken. ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.05.001
更新日期:2018-05-06 00:00:00
abstract::The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2077
更新日期:1998-05-01 00:00:00
abstract::The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2147
更新日期:1999-02-01 00:00:00
abstract::The effect of low temperatures on the ultrastructure of the plasma membrane of bull and boar spermatozoa was investigated. Cold-induced changes in the organization of sperm plasma membrane components were demonstrated by the use of fast-freezing combined with freeze-fracture electron microscopy. This preparation techn...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(90)90009-s
更新日期:1990-04-01 00:00:00
abstract::The transfusion of red blood cells from umbilical cord blood (cord RBCs) is gathering significant interest for the treatment of fetal and neonatal anemia, due to its high content of fetal hemoglobin as well as numerous other potential benefits to fetuses and neonates. However, in order to establish a stable supply of ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.04.002
更新日期:2014-06-01 00:00:00
abstract:PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2021.01.005
更新日期:2021-01-02 00:00:00
abstract::The estimation of several cellular biophysical parameters must be made in order to mathematically predict optimal cryopreservation protocols. These parameters include total cell volume, osmotically inactive volume, cell surface area, and relative water and solute permeabilities. Recent attention has been paid to the d...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.12.001
更新日期:2012-04-01 00:00:00
abstract:BACKGROUND:The number of revascularization procedures including coronary and lower extremity bypass, have increased greatly in the last decade. It suggests a growing need for vascular grafts. Cryopreserved allografts could represent a viable alternative but their immunologic reactivity remains controversial. METHODS:7...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.06.006
更新日期:2005-10-01 00:00:00
abstract::We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00123-2
更新日期:2002-10-01 00:00:00
abstract::The general belief is that slow freezing injury is either the result of exposure to high salt concentrations or the result of excessive cell shrinkage. Increased salt concentration arises as increasing amounts of pure ice precipitate out of solution during freezing and cause the liquid-filled channels in which the cel...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90016-0
更新日期:1983-06-01 00:00:00
abstract::There is increasing interest in the possibility of treating diseased or damaged areas of synovial joint surfaces by grafts of healthy allogeneic cartilage. Such grafts could be obtained from cadaver tissue donors or in the future they might be manufactured by 'tissue engineering' methods. Cartilage is an avascular tis...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.01.005
更新日期:2006-06-01 00:00:00
abstract::Cryopreservation methods for poultry semen are not reliable for germplasm preservation, especially for turkeys, where fertility rates from frozen/thawed semen are particularly low. The objective was to evaluate cryopreservation methods for effectiveness in promoting cryosurvival and post-thaw function of sperm from fi...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.04.003
更新日期:2014-06-01 00:00:00
abstract:UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1999.2181
更新日期:1999-06-01 00:00:00
abstract::Over recent years, a considerable increase in the popularity of cryostimulation and whole body cryotherapy (WBC) procedures has occurred both among healthy individuals and in various groups of patients, including those with primary untreated hypertension. The aim of this study was to compare the effects of WBC on the ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2014.07.014
更新日期:2014-10-01 00:00:00
abstract::Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.01.010
更新日期:2004-06-01 00:00:00
abstract::Addition of cholesterol-loaded cyclodextrin (CLC) to the diluents of mammalian semen increased stability and rigidity of phospholipid hydrocarbon chains of plasma membrane during sperm cryopreservation process. CLC has been tested successfully as cryoprotectant in various livestock sperm cryopreservation protocols but...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2015.01.009
更新日期:2015-04-01 00:00:00
abstract::To evaluate the effect of Ca2+ on recovery of heart function after hypothermic preservation, isolated working rat hearts were perfused continuously in different [Ca2+] (0.35-2.0 mM) at 37 degrees C for 30 min, during cooling to 5 degrees C, and then at 5 degrees C for 1, 6 or 18 h. After rewarming to 37 degrees C, con...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1994.1031
更新日期:1994-06-01 00:00:00
abstract::We have performed 128 rat liver transplants in order to examine the effect of sugars in preservation solutions on cold storage of rat livers. Glucose (Mw. 180), sucrose (Mw. 348), and raffinose (Mw. 594) were tested. Rat livers were preserved at 4 degrees C for 12, 16, 18, and 24 h in standard Eurocollins solution (EC...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(91)90051-o
更新日期:1991-10-01 00:00:00
abstract::Human blood platelets are stored in blood banks for 5 days, after which they are discarded, by federal regulation. This short lifetime has led to a chronic shortage of platelets, a problem that is particularly acute in immunosuppressed patients, such as those with AIDS. We report here that platelets can be preserved b...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2306
更新日期:2001-03-01 00:00:00