Little evidence for a major role of Ca2+ in cold-induced injury of liver cells.


:We have previously shown that cold-induced injury to hepatocytes and liver endothelial cells occurs predominantly via an iron-dependent pathway. However, other groups have reported evidences suggesting that Ca(2+) ions could be involved in the process of cold-induced injury of liver cells. We here assessed the relative importance and potential interaction of both pathways in cultured primary hepatocytes and cultured liver endothelial cells. The sequence cold incubation/rewarming of hepatocytes and endothelial cells led to an increase in the cytosolic calcium concentration during the early rewarming phase, but the increased cytosolic calcium concentration did not correlate with cell injury. A partial protection from cold-induced cell injury was achieved by the intracellular calcium chelators Quin-2 and BAPTA. However, additional experiments showed that the ability of these chelators to bind iron was probably responsible for a major part of this protection. Incubation in calcium-free media led to an increased cell injury and a physiological calcium concentration (2.5mM) was protective. In addition, targeting suggested downstream pathways of calcium-dependent cold-induced injury, i.e. by the addition of Ruthenium Red, an inhibitor of mitochondrial Ca(2+) uniporter, or by inhibiting Bax translocation to the mitochondria, did not provide protection from cold-induced injury in both cell types. Taken together, our data suggest that calcium increases but does not play a major role in cold-induced cell injury to hepatocytes and liver endothelial cells.






Knoop S,de Groot H,Rauen U




Has Abstract


2008-04-01 00:00:00














  • Relationship between equilibration times and the presence of cumulus cells, and effect of taxol treatment for vitrification of in vitro matured porcine oocytes.

    abstract::The effects of the presence or absence of cumulus cells and equilibration times (1 and 4 min) with cryoprotectant, and Taxol treatment before vitrification (0.5-5.0 microM) on development of in vitro matured porcine oocytes after vitrification were examined. Ethylene glycol (30%) and sucrose (0.5M) was used as a vitri...


    pub_type: 杂志文章


    authors: Fujihira T,Nagai H,Fukui Y

    更新日期:2005-12-01 00:00:00

  • Are nanometric films of liquid undercooled interfacial water bio-relevant?

    abstract::It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...


    pub_type: 杂志文章


    authors: Möhlmann DT

    更新日期:2009-06-01 00:00:00

  • Long-term preservation of dried phosphofructokinase by sugars and sugar/zinc mixtures.

    abstract::We have demonstrated that sugars and suger/zinc mixtures can be used to preserve the activity of dried phosphofructokinase (PFK) during long-term storage over CaSO4. After 9 weeks in the presence of either 200 mM sucrose or 200 mM trehalose little loss of PFK activity was noted, with almost 60% of the original prefree...


    pub_type: 杂志文章


    authors: Carpenter JF,Martin B,Loomis SH,Crowe JH

    更新日期:1988-08-01 00:00:00

  • The role of freezing in trypsin activity oscillations.

    abstract::The importance of the frozen phase in the formation of cryooscillations of trypsin activity has been shown in experiments conducted at -10 degrees C under frozen and supercooled conditions, respectively. A solution containing trypsin obtained by trypsinogen activation and 0.1 M MnCl2 was distributed in test tubes with...


    pub_type: 杂志文章


    authors: Vajda T

    更新日期:1992-08-01 00:00:00

  • Cryopreservation of an attenuated vaccine strain of the protozoan parasite Toxoplasma gondii.

    abstract::Toxoplasma gondii is a protozoan parasite that infects birds and mammals, including humans. T. gondii T-263 is an attenuated mutant strain that is being developed as a live vaccine to protect cats from shedding oocysts. A cryopreservation procedure for T. gondii T-263 bradyzoites has been developed to meet the require...


    pub_type: 杂志文章


    authors: Booth KS,James ER,Popiel I

    更新日期:1996-06-01 00:00:00

  • Crystallization of Ice in Aqueous Solutions of Glycerol and Dimethyl Sulfoxide. 1. A Comparison of Mechanisms

    abstract::The crystallization of ice from aqueous solutions of glycerol and dimethyl sulfoxide (Me2SO) has been studied using differential scanning calorimetry. In particular, the ice crystallization behavior of glycerol and Me2SO solutions containing approximately the same mole percent solute concentration (i.e., approximately...


    pub_type: 杂志文章


    authors: Hey JM,Macfarlane DR

    更新日期:1996-04-01 00:00:00

  • Determination of human platelet membrane permeability coefficients using the Kedem-Katchalsky formalism: estimates from two- vs three-parameter fits.

    abstract::Attempts to cryopreserve human blood platelets have resulted in poor postthaw survival rates and have been inadequate for routine clinical application. As a result, most blood banks maintain platelets in nonfrozen solutions. Using this approach, platelets can be stored for only about 5 days and are then discarded. Thi...


    pub_type: 杂志文章


    authors: Woods EJ,Liu J,Gilmore JA,Reid TJ,Gao DY,Critser JK

    更新日期:1999-05-01 00:00:00

  • Use of dynamic mechanical analysis (DMA) to determine critical transition temperatures in frozen biomaterials intended for lyophilization.

    abstract::Dynamic mechanical analysis is widely used to determine glass transitions in solid state materials. However, here we demonstrate the application of DMA for the determination of glass transitions (Tg') in the frozen liquid state by means of a steel sample pocket. The use of the pocket allows frozen material to be analy...


    pub_type: 杂志文章


    authors: Gearing J,Malik KP,Matejtschuk P

    更新日期:2010-08-01 00:00:00

  • Proteome variation of the rat liver after static cold storage assayed in an ex vivo model.

    abstract::Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver p...


    pub_type: 杂志文章


    authors: Knecht C,Balaban CL,Rodríguez JV,Ceccarelli EA,Guibert EE,Rosano GL

    更新日期:2018-12-01 00:00:00

  • Some insight into the physical basis of the cryoprotective action of dimethyl sulfoxide and ethylene glycol.

    abstract::In the determination of the solid-liquid phase equilibria in the aqueous mixtures of dimethyl sulfoxide (Me2SO) and ethylene glycol (EG) one often encounters the problem of equilibrium crystallization. In the present report the above aqueous solutions are equilibrated for crystallization in a dielectric cell during wh...


    pub_type: 杂志文章


    authors: Murthy SS

    更新日期:1998-03-01 00:00:00

  • Treatment with cholesterol-loaded methyl-β-cyclodextrin increased the cholesterol in rabbit oocytes, but did not improve developmental competence of cryopreserved oocytes.

    abstract::Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol h...


    pub_type: 杂志文章


    authors: Jiménez-Trigos E,Vicente JS,Mocé E,Naturil-Alfonso C,Fernandez-Gonzalez R,Gutierrez-Adan A,Marco-Jiménez F

    更新日期:2013-08-01 00:00:00

  • Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers.

    abstract::Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...


    pub_type: 杂志文章


    authors: Jain S,Xu H,Duncan H,Jones JW Jr,Zhang JX,Clemens MG,Lee CY

    更新日期:2004-06-01 00:00:00

  • Equilibrium vitrification of mouse embryos using low concentrations of cryoprotectants.

    abstract::Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...


    pub_type: 杂志文章


    authors: Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

    更新日期:2020-12-04 00:00:00

  • Problems of cryohemorrhoidectomy.

    abstract::One thousand cryohemorrhoidectomies were performed in the past 7 years (1973-1979). Ninety percent of the patients were satisfied with the technique; approximately one-third of the patients experienced severe to moderate pain and 11% had recurrences or related problems. The majority of patients with postoperative pain...


    pub_type: 杂志文章


    authors: Oh C

    更新日期:1982-06-01 00:00:00

  • Comparison of three methods for human corneal cryopreservation that utilize dimethyl sulfoxide.

    abstract::We compared endothelial cell survival in human corneas after cryopreservation by three methods that utilize dimethyl sulfoxide. Twenty-eight human cadaver corneas were cryopreserved by one of three methods, stored briefly over liquid nitrogen, thawed, cultured at 37 degrees C for 3 days, and fixed for scanning electro...


    pub_type: 杂志文章


    authors: Bourne WM,Nelson LR,Hodge DO

    更新日期:1999-08-01 00:00:00

  • Desiccation tolerance in bovine sperm: a study of the effect of intracellular sugars and the supplemental roles of an antioxidant and a chelator.

    abstract::Desiccation preservation holds promise as a simplified alternative to cryopreservation for the long term storage of cells. We report a study on the protective effects of intracellular and extracellular sugars during bovine sperm desiccation and the supplemental effects of the addition of an antioxidant (catalase) or a...


    pub_type: 杂志文章


    authors: Sitaula R,Elmoazzen H,Toner M,Bhowmick S

    更新日期:2009-06-01 00:00:00

  • Effectiveness of a tris-based extender (SHOTOR diluent) for the preservation of Bactrian camel (Camelus bactrianus) semen.

    abstract::The development of a suitable semen extender is required to extend artificial breeding programs and to preserve the genetic potential of Bactrian camel. Experiments were conducted to provide the optimal osmolality and pH of tris-based extender and to compare that with available extenders for short-term preservation of...


    pub_type: 杂志文章


    authors: Niasari-Naslaji A,Mosaferi S,Bahmani N,Gharahdaghi AA,Abarghani A,Ghanbari A,Gerami A

    更新日期:2006-08-01 00:00:00

  • Vitrification properties of solutions of ethylene glycol in saline containing PVP, Ficoll, or dextran.

    abstract::Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...


    pub_type: 杂志文章


    authors: Shaw JM,Kuleshova LL,MacFarlane DR,Trounson AO

    更新日期:1997-11-01 00:00:00

  • Evaluation of cell viability and apoptosis in human amniotic fluid-derived stem cells with natural cryoprotectants.

    abstract::A previous study demonstrated that disaccharides, antioxidants, and caspase inhibitors can be used in freezing solutions to reduce the concentration of Me2SO from the current standard of 10% (v/v) to 5% (v/v) or 2.5% and to eliminate fetal bovine serum (FBS) for the cryopreservation of human amniotic fluid-derived ste...


    pub_type: 杂志文章


    authors: Cho HJ,Lee SH,Yoo JJ,Shon YH

    更新日期:2014-04-01 00:00:00

  • Cellular and molecular consequences of reduced cell water content.

    abstract::Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...


    pub_type: 杂志文章


    authors: Mansell JL,Clegg JS

    更新日期:1983-10-01 00:00:00

  • 0 °C is better?- Thawing temperature optimization study for cancer cryoablation in a mouse model with green fluorescent protein-labeled Lewis lung cancer.

    abstract:PURPOSE:There are two kinds of thawing temperatures commonly adopted in cancer cryosurgery. We attempted to compare their efficacy differences in this study to optimize the surgical method. METHOD:Forty-five C57BL/6 J mice with GFP-labeled Lewis lung cancer were randomized into three groups (n = 15 for each): control ...


    pub_type: 杂志文章


    authors: Zhou T,Li Q,Chi X,Li L,Rao J,Hu K

    更新日期:2017-04-01 00:00:00

  • A re-evaluation of the role of type IV antifreeze protein.

    abstract::A lipoprotein-like antifreeze protein (type IV AFP) has previously been isolated only from the blood plasma of the longhorn sculpin. However, the plasma antifreeze activity in all individuals of this species tested from Newfoundland and New Brunswick waters ranges from low to undetectable. A close relative of the long...


    pub_type: 杂志文章


    authors: Gauthier SY,Scotter AJ,Lin FH,Baardsnes J,Fletcher GL,Davies PL

    更新日期:2008-12-01 00:00:00

  • Physiological evaluation of a rabbit kidney perfused with VS41A.

    abstract::The current report compares the renal physiological impact of a standard vitrification solution, VS41A, as measured by normothermic blood perfusion, to the physiological effects of VS4, a related but more dilute vitrification solution previously shown to be consistently compatible with life support function of transpl...


    pub_type: 杂志文章


    authors: Arnaud FG,Khirabadi B,Fahy GM

    更新日期:2003-06-01 00:00:00

  • Viability tests of cryopreserved endocrine pancreatic cells.

    abstract::Collagenase-isolated islets, which had been cultured for 1 week, were frozen at two different cooling rates. Islets frozen at 5 degrees C/min behaved from a functional point of view very similarly to that of nonfrozen, cultured control islets, except for a reduced maximal insulin secretory capacity and a reduced insul...


    pub_type: 杂志文章


    authors: Andersson A,Sandler S

    更新日期:1983-04-01 00:00:00

  • Cardiovascular and autonomic responses to whole-body cryostimulation in essential hypertension.

    abstract::Over recent years, a considerable increase in the popularity of cryostimulation and whole body cryotherapy (WBC) procedures has occurred both among healthy individuals and in various groups of patients, including those with primary untreated hypertension. The aim of this study was to compare the effects of WBC on the ...


    pub_type: 杂志文章


    authors: Zalewski P,Buszko K,Zawadka-Kunikowska M,Słomko J,Szrajda J,Klawe JJ,Tafil-Klawe M,Sinski M,Newton J

    更新日期:2014-10-01 00:00:00

  • Vitrification of in vitro produced goat blastocysts: effects of oocyte donor age and development stage.

    abstract::This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatch...


    pub_type: 杂志文章


    authors: Morató R,Romaguera R,Izquierdo D,Paramio MT,Mogas T

    更新日期:2011-12-01 00:00:00

  • Restoration of resistance to osmotic swelling of vitrified mouse embryos by short-term culture.

    abstract::In cryopreservation of mammalian embryos, embryos can be injured by osmotic swelling during removal of the cryoprotectant after warming. We have shown that vitrified embryos are more sensitive to osmotic swelling than fresh cells but that sensitivity is reduced or abolished if vitrified cells are cultured for a short ...


    pub_type: 杂志文章


    authors: Edashige K,Asano A,An TZ,Kasai M

    更新日期:1999-06-01 00:00:00

  • Magnetic resonance imaging-estimated three-dimensional temperature distribution in liver cryolesions: a study of cryolesion characteristics assumed necessary for tumor ablation.

    abstract::The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...


    pub_type: 杂志文章


    authors: Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

    更新日期:2001-11-01 00:00:00

  • Evidence for two modes of hypothermia damage in five cell lines.

    abstract::The Arrhenius plots of inactivation (killing) rates of five mammalian cell lines, V-79 Chinese hamster lung, mouse L-929, mouse neuroblastoma, human 18LU, and human erythrocytes, exposed to hypothermia contain a break somewhere between 5 and 10 degrees C caused by a change from positive to negative slope, which corres...


    pub_type: 杂志文章


    authors: Kruuv J,Glofcheski DJ,Lepock JR

    更新日期:1995-04-01 00:00:00

  • Cryopreservation of umbilical cord blood: 2. Tolerance of CD34(+) cells to multimolar dimethyl sulphoxide and the effect of cooling rate on recovery after freezing and thawing.

    abstract::Cryopreservation protocols for umbilical cord blood have been based on methods established for bone marrow (BM) and peripheral blood stem cells (PBSC). The a priori assumption that these methods are optimal for progenitor cells from UCB has not been investigated systematically. Optimal cryopreservation protocols utili...


    pub_type: 杂志文章


    authors: Hunt CJ,Armitage SE,Pegg DE

    更新日期:2003-02-01 00:00:00