Abstract：
:Mouse oocytes were cryopreserved by the vitrification method using vitrification solution (VSI) and the effects of dilution methods were examined on the rate of in vitro and in vivo development. Eighty-three percent and 75% of vitrified oocytes exhibited normal morphology when diluted in glycerol + sucrose and sucrose alone, respectively. In contrast, only 35% of the oocytes diluted by a stepwise method exhibited a normal appearance. A high proportion of vitrified oocytes was fertilized in vitro (84-94%), 80 to 87% of which were normal. Of the later embryos, 69 to 78% developed to blastocysts after 4 days of culture. Thirty-six live young (51%) were obtained when vitrified oocytes were transferred to recipient females. The overall rate of development to live young was 25% when vitrified oocytes were diluted with glycerol + sucrose solution. These results indicate that the simple and rapid procedure of vitrification and glycerol + sucrose dilution is suitable for the cryopreservation of mouse oocytes.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Kono T,Kwon OY,Nakahara Tdoi
10.1016/0011-2240(91)90007-bsubject
Has Abstractpub_date
1991-02-01 00:00:00pages
50-4issue
1eissn
0011-2240issn
1090-2392pii
0011-2240(91)90007-Bjournal_volume
28pub_type
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