Dependence of trehalose protective action on the initial phase state of dipalmitoylphosphatidylcholine bilayers.

abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...

journal_title：Cryobiology

authors： Shaik S,Wu X,Gimble JM,Devireddy R

更新日期：2020-10-01 00:00:00

abstract：:A number of living creatures in the Antarctic region have developed characteristic adaptation of cold weather by producing antifreeze proteins (AFP). Antifreeze peptide (Afp1m) fragment have been designed in the sequence of strings from native proteins. The objectives of this study were to assess the properties of Afp...

journal_title：Cryobiology

authors： Khan MS,Ibrahim SM,Adamu AA,Rahman MBA,Bakar MZA,Noordin MM,Loqman MY

更新日期：2020-02-01 00:00:00

abstract：:The importance of the frozen phase in the formation of cryooscillations of trypsin activity has been shown in experiments conducted at -10 degrees C under frozen and supercooled conditions, respectively. A solution containing trypsin obtained by trypsinogen activation and 0.1 M MnCl2 was distributed in test tubes with...

journal_title：Cryobiology

authors： Vajda T

更新日期：1992-08-01 00:00:00

abstract：:Appropriate timing of cold deacclimation is an important component of winter survival of perennial plants, such as trees, in temperate and boreal zones. Recently, concerns about predicted global climate change disturbing deacclimation timing have been increasing. The relationship between ambient temperatures and the m...

journal_title：Cryobiology

authors： Takeuchi M,Kasuga J

更新日期：2018-02-01 00:00:00

abstract：:The cold tolerance mechanisms of insect have been studied extensively on the model species Drosophila and a few other species at the transcriptional level. However studies on insects that inherit strong cold tolerance are limited. Cold hardy Tenebrionid beetle Microdera punctipennis is endemic to Gurbantonggut Desert,...

journal_title：Cryobiology

authors： Tusong K,Guo X,Meng S,Liu X,Ma J

更新日期：2017-10-01 00:00:00

abstract：:The recently formulated bioheat equation of Weinbaum and Jiji which accounts for the vascular ultrastructure and blood perfusion was applied to the freezing of peripheral tissue. Using quasi-steady approximation the temperature distribution in the two-phase tissue and the motion of the frozen front were determined. Re...

journal_title：Cryobiology

authors： Song WJ,Jiji LM

更新日期：1988-04-01 00:00:00

abstract：:Organ preservation solutions have been designed to protect grafts against the injury inflicted by cold ischemia. However, toxicity of University of Wisconsin (UW) solution during rewarming has been reported. Therefore, we here assessed the toxicity of UW, histidine-tryptophan-ketoglutarate (HTK), Euro-Collins, histidi...

journal_title：Cryobiology

authors： Rauen U,de Groot H

更新日期：2008-02-01 00:00:00

abstract：:Cell volumes, viability, ultrastructure, and metabolism have been studied in mouse L cells at reduced water contents. Dehydration was achieved by addition of sorbitol to the incubation medium at concentrations of 0.15, 0.3, and 0.6 m. In 0.3 m sorbitol, cell volume was reduced to about 35% of the initial control level...

journal_title：Cryobiology

authors： Mansell JL,Clegg JS

更新日期：1983-10-01 00:00:00

abstract：:Oocytes have been successfully cryopreserved using rapid and slow freezing procedures. However, variability in the success of replicates has limited its practical application. In the present study, mature mouse oocytes were vitrified in 6 M dimethyl sulfoxide supplemented with 1 mg/ml antifreeze glycoproteins (AFGP) (...

journal_title：Cryobiology

authors： O'Neil L,Paynter SJ,Fuller BJ,Shaw RW,DeVries AL

更新日期：1998-08-01 00:00:00

abstract：:Drosophila embryos manifest unusually high sensitivity to chilling in that they are killed with increased rapidity by exposure to temperatures between 0 and -25 degrees C in the absence of ice formation. Thus, 50% of 15-h eggs succumb in 35, 4, and 1 h at 0, -9, and -15 degrees C, respectively. The sensitivity becomes...

journal_title：Cryobiology

authors： Mazur P,Schneider U,Mahowald AP

更新日期：1992-02-01 00:00:00

abstract：:Although sperm cryopreservation is a powerful tool widely applicable in biodiversity conservation and broodstock management, cryopreservation of teleost eggs and embryos remains challenging. In the present study, we demonstrated that spermatogonia of rainbow trout (Oncorhynchus mykiss) cryopreserved for 5 years posses...

journal_title：Cryobiology

authors： Lee S,Iwasaki Y,Yoshizaki G

更新日期：2016-10-01 00:00:00

abstract：:A total of 498 porcine embryos at various stages of development collected from superovulated gilts was used to investigate cryopreservation. First, blastocysts (BL), expanded blastocysts (ExB), and hatched blastocysts (HB) were used to determine the effect of exposure to concentrated solutions of ethylene glycol as cr...

journal_title：Cryobiology

authors： Kobayashi S,Takei M,Kano M,Tomita M,Leibo SP

更新日期：1998-02-01 00:00:00

abstract：:Among the various types of stem cells, induced pluripotent stem cells (iPSCs) have gained much attention due to their pluripotent nature. iPSCs help us to understand the processes that regulate pluripotency and specialization. However, in order to use them in various applications in regenerative medicine, their effici...

journal_title：Cryobiology

authors： Fernandes S,Khan N,Kale V,Limaye L

更新日期：2019-10-01 00:00:00

abstract：:The effects of intracellular lipid polarization and lipid removal treatments on the postthawed in vitro development of frozen bovine embryos at the 8-cell stage were studied. As the first step, bovine presumptive zygotes were centrifuged at 16,000 g for 20 min for the cytoplasmic lipid polarization and their lipid lay...

journal_title：Cryobiology

authors： Murakami M,Otoi T,Sumantri C,Suzuki T

更新日期：1998-05-01 00:00:00

abstract：:Despite some 26 published reports addressing oyster sperm cryopreservation, systematic factor optimization is lacking, and sperm cryopreservation has not yet found application in aquaculture on a commercial scale. In this study, the effects of cooling rate, single or combined cryoprotectants at various concentrations,...

journal_title：Cryobiology

authors： Dong Q,Huang C,Eudeline B,Tiersch TR

更新日期：2005-10-01 00:00:00

abstract：:Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...

journal_title：Cryobiology

authors： Shaw JM,Trounson AO

更新日期：1989-10-01 00:00:00

abstract：:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, ...

journal_title：Cryobiology

authors： Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

更新日期：2004-10-01 00:00:00

abstract：:Heterocyclic derivatives of butylated hydroxytoluene (BHT) were studied as cryoprotectants of the basic media for cryopreservation of the Russian sturgeon sperm. Rates of lipid peroxidation of sturgeon sperm before and after cryopreservation were reduced in the presence of the studied compounds, exceeding the effects ...

journal_title：Cryobiology

authors： Osipova VP,Berberova NT,Gazzaeva RA,Kudryavtsev KV

更新日期：2016-04-01 00:00:00

abstract：:Maintenance of membrane fluidity is of crucial importance in ectotherms experiencing thermal changes. This maintenance has in ectotherms most often been indicated using indirect measures of biochemical changes of phospholipid membranes, which is then assumed to modulate the physico-chemical properties of the membrane....

journal_title：Cryobiology

authors： Waagner D,Bouvrais H,Ipsen JH,Holmstrup M

更新日期：2013-12-01 00:00:00

abstract：:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. ...

journal_title：Cryobiology

authors： Zeng C,Peng W,Ding L,He L,Zhang Y,Fang D,Tang K

更新日期：2014-08-01 00:00:00

abstract：:Lichens are considered freezing tolerant, although few species have been tested. Growth, a robust measure of fitness integrating processes in all partners of a lichen thallus, has not yet been used as a viability measure after freezing. We compared relative growth rates (RGR) after freezing with short-term viability m...

journal_title：Cryobiology

authors： Solhaug KA,Chowdhury DP,Gauslaa Y

更新日期：2018-06-01 00:00:00

abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...

journal_title：Cryobiology

authors： Duvivier V,Darquy S,Reach G

更新日期：1999-06-01 00:00:00

abstract：:Cryoshock is a syndrome of coagulopathy, renal, and pulmonary injury following cryotherapy, and its etiology is unknown. The aim of this study was to assess the impact of hepatic cryotherapy on renal function, and whether this effect is related to volume of cryotherapy, and to identify any predictors of renal impairme...

journal_title：Cryobiology

authors： Bagia JS,Perera DS,Morris DL

更新日期：1998-06-01 00:00:00

abstract：:The general belief is that slow freezing injury is either the result of exposure to high salt concentrations or the result of excessive cell shrinkage. Increased salt concentration arises as increasing amounts of pure ice precipitate out of solution during freezing and cause the liquid-filled channels in which the cel...

journal_title：Cryobiology

authors： Mazur P,Rigopoulos N

更新日期：1983-06-01 00:00:00

abstract：:It is known that life processes below the melting point temperature can actively evolve and establish in micrometer-sized (and larger) veins and structures in ice and permafrost soil, filled with unfrozen water. Thermodynamic arguments and experimental results indicate the existence of much smaller nanometer sized thi...

journal_title：Cryobiology

authors： Möhlmann DT

更新日期：2009-06-01 00:00:00

abstract：:There is no consensus on aspects of equine bone marrow collection and processing. The study aimed to describe the collection of large volumes of bone marrow from horses of advanced age, with emphasis on bone marrow mononuclear cells (BMMCs) recovery and viability after cryopreservation. Fourteen horses, aged 3-24 year...

journal_title：Cryobiology

authors： Bastos FZ,Barussi FCM,Santi TF,Vieira BP,Senegaglia AC,Cruz FF,Michelotto PV Jr

更新日期：2017-10-01 00:00:00

abstract：:The rapid expansion of mutant mouse colonies for biomedical research has resulted in lack of space at laboratory animal facilities and increasing risks of losing precious lines. These challenges require cheap and effective methods in addition to freezing embryos and sperm to archive the expanding mutant mouse lines. C...

journal_title：Cryobiology

authors： Huang KY,de Groot SA,Woelders H,van der Horst GT,Themmen AP,Colenbrander B,van Vlissingen JM

更新日期：2010-04-01 00:00:00

abstract：:Cryopreservation of tissue engineered bone (TEB), whilst maintaining its osteogenic ability, is imperative for large-scale clinical application. We previously reported a novel cell transplantation method, in which bone-marrow-derived mesenchymal stem cells (BMSCs) were cultured to confluence and differentiated down th...

journal_title：Cryobiology

authors： Shimizu T,Akahane M,Ueha T,Kido A,Omokawa S,Kobata Y,Murata K,Kawate K,Tanaka Y

更新日期：2013-06-01 00:00:00

abstract：:An ion beam has been used to irradiate various organisms and its effects have been studied. Because of the poor tolerance that mammalian cells have for vacuum, such studies have not been carried out on living mammalian cells until now. However, this work is important both for elucidating the mechanism of mutation in r...

journal_title：Cryobiology

authors： Feng H,Wu L,Xu A,Hu B,Hei TK,Yu Z

更新日期：2004-12-01 00:00:00

abstract：:Increasing the cell concentration during the cryopreservation of red blood cells (RBC) increased hemolysis. Similarly, increasing the cell concentration during the cryopreservation of hepatocytes reduced both the viability of the cells as assessed by trypan blue exclusion and the metabolic activity of the trypan blue-...

journal_title：Cryobiology

authors： De Loecker W,Koptelov VA,Grischenko VI,De Loecker P

更新日期：1998-09-01 00:00:00