当前位置： SCI文献检索 > CRYOBIOLOGY期刊下所有文献 > Activation of AMP-dependent protein kinase by hypoxia and hypothermia in the liver of frog Rana perezi.

Activation of AMP-dependent protein kinase by hypoxia and hypothermia in the liver of frog Rana perezi.

Abstract：

:We have investigated different signaling molecules that could be activated by temperature acclimation and hypoxia, using an experimental approach consisting in submerging frogs in a water-filled box maintained at 2-4 degrees C at ambient oxygen levels or supplied with 98% N2:2% CO2 for normoxia or hypoxia conditions, respectively. The results obtained showed no significant changes in the expression of heat shock protein 70. The phosphorylation state of AMP-dependent activated protein kinase, the down-stream component of a protein kinase cascade that acts as an intracellular energy sensor, was significantly increased in both experimental conditions, showing higher values in the absence of oxygen. Similarly, the phosphorylation state of one of its known substrates, elongation factor 2, was also increased, consistent with the arrest of protein synthesis. These results point out an important role of this kinase, adjusting the rates of ATP-consuming and ATP-generating pathways, in the survival strategies to hypoxia and hypothermia.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Bartrons M,Ortega E,Obach M,Calvo MN,Navarro-Sabaté A,Bartrons R

doi

10.1016/j.cryobiol.2004.06.004

subject

Has Abstract

pub_date

2004-10-01 00:00:00

pages

190-4

issue

2

eissn

0011-2240

issn

1090-2392

pii

S0011-2240(04)00104-X

journal_volume

49

pub_type

杂志文章

相关文献

CRYOBIOLOGY文献大全
  • Freezing monolayers of cells without gap junctions.

    abstract：:Cells in monolayers have been reported to be more susceptible to freezing injury than the same cell type frozen in dispersed suspensions. There appears to be an enhanced susceptibility to intracellular freezing in the monolayers, which is thought to be facilitated by the presence of gap junctions allowing the spread o...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(03)00017-8

    authors： Armitage WJ,Juss BK

    更新日期：2003-04-01 00:00:00

  • Cryoprotection of red blood cells by a 2,3-butanediol containing mainly the levo and dextro isomers.

    abstract：:A 2,3-butanediol containing 96.7% (w/w) racemic mixture of the levo and dextro isomers and only 3.1% (w/w) of the meso isomer (called 2,3-butanediol 97% dl) has been used for the cryoprotection of red blood cells. The erythrocytes were cooled to -196 degrees C at rates between 2 and 3500 degrees C/min, followed by slo...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/0011-2240(92)90036-2

    authors： Boutron P

    更新日期：1992-06-01 00:00:00

  • Hepatic cold hypoxia and oxidative stress: implications for ICAM-1 expression and modulation by glutathione during experimental isolated liver preservation.

    abstract：:Cold preservation and reperfusion of liver during transplantation are necessary steps in the procedure but which are also associated with damage to the organ. One aspect of this damage is thought to concern up-regulation of inflammatory markers, such as the adhesion molecule intercellular adhesion molecule 1 (ICAM-1) ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2003.09.003

    authors： El-Wahsh M,Fuller B,Davidson B,Rolles K

    更新日期：2003-10-01 00:00:00

  • Thermal study of simple amino-alcohol solutions.

    abstract：:Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(02)00017-2

    authors： Baudot A,Cacela C,Duarte ML,Fausto R

    更新日期：2002-04-01 00:00:00

  • Current techniques and the future of lung preservation.

    abstract：:Although lung transplant remains the only option for patients suffering from end-stage lung failure, donor supply is insufficient to meet demand. Static cold preservation is the most common method to preserve lungs in transport to the recipient; however, this method does not improve lung quality and only allows for 8 ...

    journal_title：Cryobiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.cryobiol.2020.04.009

    authors： Lautner LJ,Freed DH,Nagendran J,Acker JP

    更新日期：2020-06-01 00:00:00

  • Ex-vivo study of flow dynamics and endothelial cell structure during extended hypothermic machine perfusion preservation of livers.

    abstract：:Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2004.01.010

    authors： Jain S,Xu H,Duncan H,Jones JW Jr,Zhang JX,Clemens MG,Lee CY

    更新日期：2004-06-01 00:00:00

  • Comparison of four different permitting and combination of two best cryoprotectants on freezing Nguni sperm evaluated with the aid of computer aided sperm analysis.

    abstract：:Cryopreservation has been reported to damage approximately 40-50% of viable sperm in bull semen. The present study was undertaken to assess the cryo-effectiveness of glycerol (GLY), ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propylene glycol (PND) as cryoprotectant during the cryopreservation of Nguni bull se...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2016.04.001

    authors： Seshoka MM,Mphaphathi ML,Nedambale TL

    更新日期：2016-06-01 00:00:00

  • Equilibrium vitrification of mouse embryos using low concentrations of cryoprotectants.

    abstract：:Previously, we developed a method for vitrification of mouse embryos in a near-equilibrium state using EFS35c, PB1 medium containing 35% (v/v) ethylene glycol, and 0.98 M sucrose. This method has advantages in both slow freezing and vitrification. However, since the vitrification solution in this method contains high ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.11.015

    authors： Qiu J,Hasegawa A,Mochida K,Ogura A,Koshimoto C,Matsukawa K,Edashige K

    更新日期：2020-12-04 00:00:00

  • Hatching, survival and deformities of piracanjuba (Brycon orbignyanus) embryos subjected to different cooling protocols.

    abstract：:Groups of one hundred Brycon orbignyanus embryos at the stage of blastopore closure were subjected to different cooling protocols. Different combinations and concentrations of cryoprotectants were tested: sucrose, methanol, ethylene glycol and dimethyl sulfoxide (Me2SO); at different temperatures (0.0±2.0°C and 8.0±2....

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.10.003

    authors： Paes Mdo C,da Silva RC,do Nascimento NF,Valentin FN,Senhorini JA,Nakaghi LS

    更新日期：2014-12-01 00:00:00

  • Cryopreservation of Radopholus similis, a tropical plant-parasitic nematode.

    abstract：:For obligate plant-parasitic nematodes, cryopreservation has advantages over the usual preservation methods on whole plants or axenic culture systems, because the latter two are labourious and time and space consuming. In addition, cross contamination among different isolates can occur easily. Moreover, specific genet...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2007.06.006

    authors： Elsen A,Vallterra SF,Van Wauwe T,Thuy TT,Swennen R,De Waele D,Panis B

    更新日期：2007-10-01 00:00:00

  • The effect of cooling rate and temperature on the toxicity of ethylene glycol in the rabbit internal carotid artery.

    abstract：:The smooth muscle and vascular endothelium of small elastic arteries (the rabbit common carotid artery) are injured by exposure to 40% ethylene glycol (EG) at 4 degrees C, and additional damage occurs when the arteries are cooled without freezing to -20 degrees C. This paper reports attempts to reduce this injury by a...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1996.0042

    authors： Wusteman M,Boylan S,Pegg DE

    更新日期：1996-08-01 00:00:00

  • Cathepsin activities and membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling.

    abstract：:This study investigated enzymatic activity of cathepsins and the membrane integrity of zebrafish (Danio rerio) oocytes after freezing to -196 degrees C using controlled slow cooling. Stage III oocytes (>0.5mm), obtained through dissection of anaesthetised female fish and desegregation of ovarian cumulus, were exposed ...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.01.002

    authors： Zhang T,Rawson DM,Tosti L,Carnevali O

    更新日期：2008-04-01 00:00:00

  • The effect of liposomes on thermotropic membrane phase transitions of bovine spermatozoa and oocytes: implications for reducing chilling sensitivity.

    abstract：:We have examined the effects of combinations between egg-phosphatidylcholine (EPC) or dipalmitoylphosphatidylcholine (DPPC) liposomes with either bovine spermatozoa or oocytes on cellular chilling sensitivity, lipid phase transition temperature (T(m)), and the ability of the oocytes to develop to the blastocyst stage....

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/s0011-2240(02)00123-2

    authors： Zeron Y,Tomczak M,Crowe J,Arav A

    更新日期：2002-10-01 00:00:00

  • Machine perfusion enhances hepatocyte isolation yields from ischemic livers.

    abstract：BACKGROUND:High-quality human hepatocytes form the basis of drug safety and efficacy tests, cell-based therapies, and bridge-to-transplantation devices. Presently the only supply of cells derives from an inadequate pool of suboptimal disqualified donor livers. Here we evaluated whether machine perfusion could ameliorat...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2015.07.006

    authors： Izamis ML,Perk S,Calhoun C,Uygun K,Yarmush ML,Berthiaume F

    更新日期：2015-10-01 00:00:00

  • Effects of lyophilization on the stability of bacteriophages against different serogroups of Shiga toxin-producing Escherichia coli.

    abstract：:Lyophilization is commonly used to effectively preserve the stability of bacteriophages (phages) in long-term storage. However, information regarding the lyophilization of phages specific to Shiga toxin-producing Escherichia coli (STEC) strains is scarce. The objective of this study was to determine the effects of lyo...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.07.012

    authors： Lavenburg VM,Liao YT,Salvador A,Hsu AL,Harden LA,Wu VCH

    更新日期：2020-10-01 00:00:00

  • Cryopreservation of specific pathogen-free (SPF) pig islet cells: effect of culture time before cryopreservation and after thawing.

    abstract：UNLABELLED:The aim of this study was to determine the optimal conditions (effect of culture time before and after cryopreservation) for cryopreservation of specific pathogen-free pig islet cells. METHODS:(1) Glucose-induced insulin secretion by fresh islet cells cultured for 10 days was compared to that by islet cells...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1999.2181

    authors： Duvivier V,Darquy S,Reach G

    更新日期：1999-06-01 00:00:00

  • Development of a spermatogonia cryopreservation protocol for blue catfish, Ictalurus furcatus.

    abstract：:Sustainability of channel catfish, Ictalurus punctatus ♀ × blue catfish, Ictalurus furcatus ♂ hybrid aquaculture relies on new innovative technologies to maximize fry output. Transplanting spermatogonial stem cells (SSCs) from blue catfish into channel catfish hosts has the potential to greatly increase gamete availab...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.10.010

    authors： Abualreesh M,Myers JN,Gurbatow J,Johnson A,Xing,Wang J,Li S,Coogan M,Vo K,El Husseini N,Dunham RA,Butts IAE

    更新日期：2020-12-01 00:00:00

  • Effect of two cooling protocols on the post-thaw characteristics of Iberian ibex sperms.

    abstract：:The rate at which lethal intracellular ice forms during sperm cryopreservation is highly dependent on the cooling protocol. The present work compares two cooling protocols for use with Iberian ibex (Capra pyrenaica) sperm by assessing the effects on the motility, viability, and size of frozen-thawed sperm cells. Ejacu...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.01.003

    authors： Esteso MC,Toledano-Díaz A,Castaño C,Pradiee J,Lopez-Sebastián A,Santiago-Moreno J

    更新日期：2018-02-01 00:00:00

  • In vitro fertilization of ovine oocytes vitrified by solid surface vitrification at germinal vesicle stage.

    abstract：:Cryopreservation of immature oocytes at germinal vesicle (GV) stage would provide a readily available source of oocytes for use in research and allow experiments to be performed irrespective of seasonality or other constraints. This study was designed to evaluate the recovery, viability, maturation status, fertilizati...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2012.04.008

    authors： Moawad AR,Fisher P,Zhu J,Choi I,Polgar Z,Dinnyes A,Campbell KH

    更新日期：2012-10-01 00:00:00

  • Non-toxic freezing media to retain the stem cell reserves in adipose tissues.

    abstract：:Subcutaneous adipose tissue is a rich source of stromal vascular fraction (SVF) and adipose-derived stromal/stem cells (ASCs) that are inherently multipotent and exhibit regenerative properties. In current practice, lipoaspirate specimens harvested from liposuction surgeries are routinely discarded as a biohazard wast...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.07.005

    authors： Shaik S,Wu X,Gimble JM,Devireddy R

    更新日期：2020-10-01 00:00:00

  • Development of a freeze-drying protocol for the long-term storage of S9-fraction at ambient temperatures.

    abstract：:The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2008.11.007

    authors： Buchinger S,Campen E,Helmers E,Morosow V,Krefft M,Reifferscheid G

    更新日期：2009-04-01 00:00:00

  • Successful recovery of motility and fertility of cryopreserved cane toad (Bufo marinus) sperm

    abstract：:The recent decline and extinction of amphibian species is a worldwide phenomenon without an identified cause or solution. Assisted reproductive technologies, including sperm cryopreservation, are required to manage endangered amphibian species and preserve their genetic diversity. This study on the Anuran amphibian (B...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.1998.2129

    authors： Browne RK,Clulow J,Mahony M,Clark A

    更新日期：1998-12-01 00:00:00

  • Proteome variation of the rat liver after static cold storage assayed in an ex vivo model.

    abstract：:Cold storage is a common procedure for liver preservation in a transplant setting. However, during cold ischemia, the liver suffers molecular alterations that can affect its performance. Also, deleterious mechanisms set forth in the storage phase are exacerbated during reperfusion. This study aimed to identify liver p...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2018.10.003

    authors： Knecht C,Balaban CL,Rodríguez JV,Ceccarelli EA,Guibert EE,Rosano GL

    更新日期：2018-12-01 00:00:00

  • Evaluation of cell viability and apoptosis in human amniotic fluid-derived stem cells with natural cryoprotectants.

    abstract：:A previous study demonstrated that disaccharides, antioxidants, and caspase inhibitors can be used in freezing solutions to reduce the concentration of Me2SO from the current standard of 10% (v/v) to 5% (v/v) or 2.5% and to eliminate fetal bovine serum (FBS) for the cryopreservation of human amniotic fluid-derived ste...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2014.02.003

    authors： Cho HJ,Lee SH,Yoo JJ,Shon YH

    更新日期：2014-04-01 00:00:00

  • Hypothermia produces rat liver proteomic changes as in hibernating mammals but decreases endoplasmic reticulum chaperones.

    abstract：:Hypothermia is used in the clinic for protection of organs such as the brain against ischemic injury during aortic/complex congenital cardiac surgery or post-resuscitation encephalopathy. The principal mechanism of hypothermic protection is suppression of metabolism, however, the pleiotropic effects of cooling are inc...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2012.05.004

    authors： Oda T,Shimizu K,Yamaguchi A,Satoh K,Matsumoto K

    更新日期：2012-10-01 00:00:00

  • Effects of kinetin supplementation on the post-thaw motility, viability, and structural integrity of dog sperm.

    abstract：:Oxidative stress is one of the major issues associated with cryopreservation because it causes a marked reduction in the post-thaw quality of semen. This study investigated the ability of kinetin to preserve the structural and functional integrity of dog sperm during cryopreservation. Pooled ejaculates were divided in...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2020.05.015

    authors： Qamar AY,Fang X,Bang S,Kim MJ,Cho J

    更新日期：2020-08-01 00:00:00

  • The future of cryoablation: An abscopal effect.

    abstract：:Cryoablation has become a popular modality to treat a variety of malignant tumors in solid organs and soft tissues. In the future, the use of cryoablation should focus on its abscopal effect. The present review discusses the increased immune response triggered by cryoablation alone or by cryoablation combined with imm...

    journal_title：Cryobiology

    pub_type： 杂志文章,评审

    doi：10.1016/j.cryobiol.2020.02.010

    authors： Chen J,Qian W,Mu F,Niu L,Du D,Xu K

    更新日期：2020-12-01 00:00:00

  • Inherent toxicity of organ preservation solutions to cultured hepatocytes.

    abstract：:Organ preservation solutions have been designed to protect grafts against the injury inflicted by cold ischemia. However, toxicity of University of Wisconsin (UW) solution during rewarming has been reported. Therefore, we here assessed the toxicity of UW, histidine-tryptophan-ketoglutarate (HTK), Euro-Collins, histidi...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2007.09.003

    authors： Rauen U,de Groot H

    更新日期：2008-02-01 00:00:00

  • Vitrification of in vitro produced goat blastocysts: effects of oocyte donor age and development stage.

    abstract：:This study examines the effectiveness of the cryotop vitrification method for the cryopreservation of goat blastocysts. To determine the effects of embryo development stage and donor age on in vitro survival rates, good-quality blastocysts from adult and prepubertal goats were sorted into non-expanded, expanded, hatch...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1016/j.cryobiol.2011.09.002

    authors： Morató R,Romaguera R,Izquierdo D,Paramio MT,Mogas T

    更新日期：2011-12-01 00:00:00

  • Magnetic resonance imaging-estimated three-dimensional temperature distribution in liver cryolesions: a study of cryolesion characteristics assumed necessary for tumor ablation.

    abstract：:The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...

    journal_title：Cryobiology

    pub_type： 杂志文章

    doi：10.1006/cryo.2001.2351

    authors： Mala T,Samset E,Aurdal L,Gladhaug I,Edwin B,Søreide O

    更新日期：2001-11-01 00:00:00