Abstract：
:Artificial insemination (AI) with cryopreserved boar semen is limited to no more than 1% of the total number of inseminations due to low conception rates and litter sizes. Cryopreservation causes a dramatic decrease in the viability, motility and fertility of spermatozoa, but the underlying mechanism remains unknown. In this study, mRNA expression and protein levels of epigenetic-related genes (Dnmt3a, Dnmt3b, Jhdm2a, Kat8, Prm1, Prm2 and IGF2) in fresh and cryopreserved boar spermatozoa were evaluated using qRT-PCR and ELISA. The results showed that cryopreservation or freezing, which drastically alter the environmental stimuli, can induce epigenetic changes of boar spermatozoa. Dramatic changes of mRNA expression of epigenetic-related genes were observed before and after cryopreservation, and low protein levels of multiple genes were mainly found in program freezing groups with or without LEY. The addition of different cryoprotective agents to the freezing extender can provide better protective effects for boar spermatozoa to avoid freezing or cryopreservation-induced expression changes of epigenetic-related genes.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Zeng C,Peng W,Ding L,He L,Zhang Y,Fang D,Tang Kdoi
10.1016/j.cryobiol.2014.06.003subject
Has Abstractpub_date
2014-08-01 00:00:00pages
119-27issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(14)00124-2journal_volume
69pub_type
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