Abstract:
:The distribution of cryoprotectant (10% glycerol) and ice along the frozen plastic straw (the most useful container for freezing mammalian semen, oocytes and embryos) was studied by Raman scattering technique. Raman spectroscopy being a contactless, non-invasive tool was applied for the straws filled with the cryoprotectant solution and frozen by controlled rate programs commonly used for mammalian embryos freezing. Analysis of Raman spectra measured at different points along the straw reveals a non-uniform distribution of the cryoprotectant. The ratio between non-crystalline solution and ice was found to be increased by several times at the bottom side of the solution column frozen by the standard freezing program. The increase of the cryoprotectant fraction occurs in the area where embryos or oocytes are normally placed during their freezing. Possible effects of the cooling rate and the ice nucleation temperature on the cryoprotectant fraction at the bottom side of the solution column were considered. Our findings highlight that the ice fraction around cryopreserved embryos or oocytes can differ significantly from the averaged one in the frozen plastic straws.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Karpegina YA,Okotrub KA,Brusentsev EY,Amstislavsky SY,Surovtsev NVdoi
10.1016/j.cryobiol.2016.01.002subject
Has Abstractpub_date
2016-04-01 00:00:00pages
148-53issue
2eissn
0011-2240issn
1090-2392pii
S0011-2240(16)30002-5journal_volume
72pub_type
杂志文章相关文献
CRYOBIOLOGY文献大全abstract::Widely regarded as the most promising approach to long-term cryopreservation of organs for transplantation, vitrification is a process where liquid is transformed into a disordered solid state free from crystals, known as the amorphous state. The vitreous state is obtained by rapid cooling to cryogenic temperatures in...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/s0011-2240(02)00017-2
更新日期:2002-04-01 00:00:00
abstract::The antimalarial drug artemisinin has been found in transformed (hairy) roots of Artemisia annua. A protocol was developed to preserve A. annua hairy roots in liquid nitrogen. Root tips were excised from 7-day-old cultures and held on solid White's medium for 24 h prior to cryoprotection. They were then treated with a...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1996.0011
更新日期:1996-02-01 00:00:00
abstract::The susceptibility of in vitro matured (IVM) porcine oocytes to be fertilized in vitro after vitrification was investigated. IVM oocytes were cryopreserved by solid surface vitrification (SSV) or treated with cryoprotectants (toxicity control, TC). Control oocytes were not treated or vitrified. Live oocytes in the thr...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2007.06.008
更新日期:2007-10-01 00:00:00
abstract::The purpose of this study was to investigate the long-term stabilization of the liver S9-fraction that is widely used in genotoxicity assays in order to mimic bio-activating processes of xenobiotics in vitro. A successful long-term stabilization of the S9-fraction meets the growing demand for the construction of a lab...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.11.007
更新日期:2009-04-01 00:00:00
abstract::Dry preservation has been explored as an energy-efficient alternative to cryopreservation, but the high sensitivity of mammalian cells to desiccation stress has been one of the major hurdles in storing cells in the desiccated state. An important strategy to reduce desiccation sensitivity involves use of the disacchari...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2011.11.007
更新日期:2012-04-01 00:00:00
abstract::Renal epithelial cells from donor kidneys are susceptible to hypothermic preservation injury, which is attenuated when they over express the cytoskeletal linker protein ezrin. This study was designed to characterize the mechanisms of this protection. Renal epithelial cell lines were created from LLC-PK1 cells, which e...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2012.04.003
更新日期:2012-08-01 00:00:00
abstract::Cryopreservation and storage of germplasm is an important factor in the prevention of disease transmission by embryo transfer and artificial insemination. Here we report the results of an investigation on transmission of selected bacterial and viral pathogens by the vapour phase of liquid nitrogen (VPLN) to embryos an...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.12.004
更新日期:2005-04-01 00:00:00
abstract::The objectives of this study were to identify the presence of different spermatozoa subpopulations (SPs) according to their kinematic characteristics in the sperm of common carp and to test the effects of cryopreservation and prolonged (6-day) storage at room temperature (RT; 23 °C) and 4 °C on spermatozoa motility an...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2021.01.007
更新日期:2021-01-12 00:00:00
abstract::Two-cell mouse embryos were frozen by direct plunging into liquid nitrogen after a 3-min exposure to solutions containing 0.25 M sucrose with 1.5, 3, or 4.5 M dimethyl sulfoxide (Me2SO), and 0, 4, 8, 16, or 32 mg/ml bovine serum albumin (BSA). In the absence of BSA, significantly more embryos were lost or damaged duri...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(89)90066-7
更新日期:1989-10-01 00:00:00
abstract::Intracerebral grafting of immature brain tissue is now widely used as a tool to study neuronal development and regeneration in the brain and spinal cord. This has stimulated the interest in methods for storage of such tissue before transplantation. In this study a method for cryopreservation of immature rat central ne...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(87)90014-9
更新日期:1987-04-01 00:00:00
abstract::1,3-Butanediol and 2,3-butanediol have been used in buffered solutions with 20, 30, or 35% (w/w) alcohol to cool erythrocytes to -196 degrees C at different cooling rates between 1 to 3500 degrees C/min, followed by slow or rapid rewarming. 1,3-butanediol shows the same shapes of red blood cell survival curves as 1,2-...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(88)90019-3
更新日期:1988-02-01 00:00:00
abstract::The effect of cryopreservation on the ability of primary human cancer cells to form colonies in a two-layer agar system was examined. Although considerable variation occurred, concentrations of 5 or 10% dimethylsulfoxide employed with slow freezing rates allowed survival of colonies in the range 20-40% or greater of n...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(84)90215-3
更新日期:1984-04-01 00:00:00
abstract::Vitrification solutions which are used for cells or embryos generally contain cryoprotectant, physiological saline, and one or more macromolecular solutes. The macromolecules modify the vitrification tendencies of these solutions, but there is little detailed information on the vitrification properties of ethylene gly...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1997.2043
更新日期:1997-11-01 00:00:00
abstract::Ibex spermatozoa can be successfully frozen using glycerolated media. However, no information is available regarding the most effective method of glycerol addition in this species. The aim of the present work was to evaluate the effect of the glycerolization temperature on the response to freezing-thawing of ibex sper...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2010.07.005
更新日期:2010-10-01 00:00:00
abstract::The site of ice nucleation in the freeze-tolerant, sub-Antarctic beetle Hydromedion sparsutum has been investigated. Ice+ bacteria, active at above -2.0 degrees C, were isolated from the guts of beetles and identified as a fluorescent Pseudomonas species. Other possible sites of nucleation, including the hemolymph, we...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1998.2147
更新日期:1999-02-01 00:00:00
abstract::The goal of this study was to estimate the three-dimensional (3D) temperature distribution in liver cryolesions and assess the margin of the transition zone between the tumoricidal core of the lesion and the surrounding unfrozen tissue, using criteria proposed in the literature. Local recurrences after liver tumor cry...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2351
更新日期:2001-11-01 00:00:00
abstract::We make some crucial remarks about the recent review by Buitink and Leprince (2004) [Cryobiology 48 (2004) 215-228] considering their false definition about the glass transition temperature based on the value of (shear) viscosity: 1014 Pa⋅s. The correct value is 1012 Pa⋅s as evidenced from http://goldbook.iupac.org/ht...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2018.07.002
更新日期:2018-08-01 00:00:00
abstract::One of the recent approaches to enhance desiccation tolerance in red blood cells (RBCs) is by loading trehalose. This process has been shown to increase the recovery of lyophilized RBCs; conversely, it results in cellular damage including hemoglobin oxidation and loss of membrane integrity. The purpose of this study w...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2008.12.003
更新日期:2009-04-01 00:00:00
abstract::Attempts to cryopreserve fish embryos have been conducted over the past three decades, nevertheless successful cryopreservation protocol for long-term storage still remains elusive. Fish oocytes offer some advantages when compared to embryos, which may help in improving the chances of cryopreservation. In the present ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2013.10.002
更新日期:2013-12-01 00:00:00
abstract::The ability to desiccate mammalian cells while maintaining a high degree of viability would have implications for many areas of biological science, including tissue engineering. Previously, we reported that introduction of the genes for trehalose biosynthesis allowed human cells in culture to be reversibly desiccated ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2324
更新日期:2001-05-01 00:00:00
abstract::This study examined the potential for "cryoimmunology" to increase the destruction of the Dunning AT-1 prostate tumor after cryosurgery. Two possible mechanisms explaining the cryoimmunologic response were studied. The first was that an antitumor antibody is produced after cryosurgery. The second was that freezing ind...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.2001.2305
更新日期:2001-02-01 00:00:00
abstract::Extremophilic lichens and their photosynthesizing photobionts from the cold regions of Earth are adapted to perform photosynthesis at subzero temperatures. To evaluate interspecific differences in the critical temperature for primary photochemical processes of photosynthesis, we exposed lichen thalli of Usnea antarcti...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2016.10.002
更新日期:2016-12-01 00:00:00
abstract::We tested a "standard" cryopreservation protocol (slow cooling with 10% DMSO) on the human embryonic stem cell (hESC) line H9 containing an Oct-4 (POU5F1) promoter-driven, enhanced green fluorescent protein (EGFP) reporter to monitor maintenance of pluripotency. Cells were cooled to -80 degrees C in cryovials and then...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2006.05.005
更新日期:2006-10-01 00:00:00
abstract::We have investigated the cryoprotective effect of alcohols on Saccharomyces cerevisiae and other yeast under various freezing conditions. For S. cerevisiae, at a cooling rate of 3 degrees C min-1 methanol and ethanol acted as cryosensitizers. However, at a cooling rate of 200 degrees C min-1, both methanol and ethanol...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1006/cryo.1994.1023
更新日期:1994-04-01 00:00:00
abstract::Liver transplantation is often the only effective treatment for end stage liver diseases resulting from cirrhosis, hepatitis, progressive jaundice, and biliary atresia. Hypothermic machine perfusion (HMP) preservation may enhance donor pool by extending preservation time and reclaiming marginal donor livers including ...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2004.01.010
更新日期:2004-06-01 00:00:00
abstract::Previous studies have shown that there are differences between hibernators and nonhibernators in the susceptibility to ventricular fibrillation. In an attempt to clarify these differences ventricular fibrillation was induced in isolated hearts of the hibernator, the woodchuck, Marmota monax by cooling, warming, punctu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(83)90031-7
更新日期:1983-08-01 00:00:00
abstract:PURPOSE:The aim is to report the first live-birth following ICSI using spermatozoa previously vitrified in a Stripper Tip. PRINCIPAL RESULTS:A 34-year-old cryptozoospermic man was enrolled in a sperm vitrification program. After failure of conventional freezing technique, spermatozoa were vitrified using two carriers:...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2021.01.005
更新日期:2021-01-02 00:00:00
abstract::Freeze-tolerant third instar larvae of the gallfly Eurosta solidaginis were cooled at 10, 5, 1, and 0.1 degrees C min-1 to -40 degrees C and then warmed to +5 degrees C at 1 degree C min-1. After cooling and warming the larvae were transferred to 21 degrees C and the survival of larvae, success of pupariation, and adu...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(89)90024-2
更新日期:1989-06-01 00:00:00
abstract::We investigated cryopreservation of horizontal transmission stages of Holospora undulata, a micronucleus-specific bacterial parasite of Paramecium caudatum. Unlike in previous studies on related Holospora species, protocols using glycerol as cryoprotectant failed entirely. In contrast, freezing with dimethyl sulfoxide...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/j.cryobiol.2005.10.014
更新日期:2006-02-01 00:00:00
abstract::The cause of slow freezing injury and the basis of the protection by solutes like glycerol are subjects of debate. During slow freezing, cells are sequestered in unfrozen channels between ice crystals that grow by removing pure water from the channels. As a consequence, the solute concentration in the channels rises a...
journal_title:Cryobiology
pub_type: 杂志文章
doi:10.1016/0011-2240(89)90030-8
更新日期:1989-02-01 00:00:00