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The effects of different concentrations of glycerol and dimethylsulfoxide on the metabolic activities of kidney slices.

Abstract：

:Kidney slices either were exposed to the cryoprotectants for 1 hr at room temperature and subsequently washed and incubated in fresh KR buffer containing only the radioactive metabolic tracers, or were immediately incubated for 2 hr at 37 degrees C in KR buffer containing the cryoprotectant and the tracers. Exposure to glycerol by incubation of kidney slices in Krebs-Ringer bicarbonate buffer containing varying concentrations of glycerol from 0 to 70% (v/v) resulted in a pronounced inhibitory effect on the protein synthesizing activity, while thymidine incorporation into DNA and the alpha-aminoisobutyric acid uptake through the cell membranes were less affected. Exposure of the tissue to buffer containing dimethylsulfoxide (Me2SO) in concentrations of 10 to 20% (v/v) resulted in a stimulatory effect on metabolism. At higher concentrations, Me2SO was toxic resulting in damaging effects on protein and DNA synthesis as well as on membrane integrity. The stimulatory effects of exposure to low concentrations of Me2SO on protein and DNA synthesis in kidney slices were concluded to be the result of an increased transport of precursors through the cell membranes.

journal_name

Cryobiology

journal_title

Cryobiology

authors

Penninckx F,Cheng N,Kerremans R,Van Damme B,De Loecker W

doi

10.1016/0011-2240(83)90059-7

subject

Has Abstract

pub_date

1983-02-01 00:00:00

pages

51-60

issue

1

eissn

0011-2240

issn

1090-2392

pii

0011-2240(83)90059-7

journal_volume

20

pub_type

杂志文章

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