Abstract:
:Membrane cholesterol:phospholipids ratio is an important determinant of cell chilling sensitivity. At low temperatures, major membrane destabilisation occurs when the membrane undergoes a phase transition. To increase membrane fluidity and stability during cooling and thus increase oocyte cryoresistance, cholesterol has been added to the plasma membrane. This study was conducted to determine if cholesterol could be incorporated into rabbit oocytes by incubation with cholesterol-loaded methyl-β-cyclodextrin (CLC) and if added cholesterol could improve the developmental ability of cryopreserved oocytes after parthenogenetic activation or intracytoplasmic sperm injection. Fresh, frozen and vitrified oocytes incubated with CLC containing 20% NBD-labelled cholesterol (NBD-CLC) were evaluated using confocal microscopy. Fluorescence intensity was higher in fresh oocytes than in cryopreserved ones. Pre-treating rabbit oocytes with 1mg of NBD-CLC/mL did not improve cleavage and developmental rates after cryopreservation. Results showed that treatment with CLC increased the cytoplasmic cholesterol content, but did not improve cleavage rate and developmental competence of cryopreserved oocytes.
journal_name
Cryobiologyjournal_title
Cryobiologyauthors
Jiménez-Trigos E,Vicente JS,Mocé E,Naturil-Alfonso C,Fernandez-Gonzalez R,Gutierrez-Adan A,Marco-Jiménez Fdoi
10.1016/j.cryobiol.2013.05.006subject
Has Abstractpub_date
2013-08-01 00:00:00pages
106-8issue
1eissn
0011-2240issn
1090-2392pii
S0011-2240(13)00158-2journal_volume
67pub_type
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