Abstract：
:Genome mining has become an increasingly powerful, scalable, and economically accessible tool for the study of natural product biosynthesis and drug discovery. However, there remain important biological and practical problems that can complicate or obscure biosynthetic analysis in genomic and metagenomic sequencing projects. Here, we focus on limitations of available technology as well as computational and experimental strategies to overcome them. We review the unique challenges and approaches in the study of symbiotic and uncultured systems, as well as those associated with biosynthetic gene cluster (BGC) assembly and product prediction. Finally, to explore sequencing parameters that affect the recovery and contiguity of large and repetitive BGCs assembled de novo, we simulate Illumina and PacBio sequencing of the Salinispora tropica genome focusing on assembly of the salinilactam (slm) BGC.
journal_name
Mar Drugsjournal_title
Marine drugsauthors
Miller IJ,Chevrette MG,Kwan JCdoi
10.3390/md15060165subject
Has Abstractpub_date
2017-06-06 00:00:00issue
6issn
1660-3397pii
md15060165journal_volume
15pub_type
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