Regional susceptibility to domoic acid in primary astrocyte cells cultured from the brain stem and hippocampus.


:Domoic acid is a marine biotoxin associated with harmful algal blooms and is the causative agent of amnesic shellfish poisoning in marine animals and humans. It is also an excitatory amino acid analog to glutamate and kainic acid which acts through glutamate receptors eliciting a very rapid and potent neurotoxic response. The hippocampus, among other brain regions, has been identified as a specific target site having high sensitivity to DOM toxicity. Histopathology evidence indicates that in addition to neurons, the astrocytes were also injured. Electron microscopy data reported in this study further supports the light microscopy findings. Furthermore, the effect of DOM was confirmed by culturing primary astrocytes from the hippocampus and the brain stem and subsequently exposing them to domoic acid. The RNA was extracted and used for biomarker analysis. The biomarker analysis was done for the early response genes including c-fos, c-jun, c-myc, Hsp-72; specific marker for the astrocytes- GFAP and the glutamate receptors including GluR 2, NMDAR 1, NMDAR 2A and B. Although, the astrocyte-GFAP and c-fos were not affected, c-jun and GluR 2 were down-regulated. The microarray analysis revealed that the chemokines / cytokines, tyrosine kinases (Trk), and apoptotic genes were altered. The chemokines that were up-regulated included - IL1-alpha, IL-Beta, IL-6, the small inducible cytokine, interferon protein 10P-10, CXC chemokine LIX, and IGF binding proteins. The Bax, Bcl-2, Trk A and Trk B were all down-regulated. Interestingly, only the hippocampal astrocytes were affected. Our findings suggest that astrocytes may present a possible target for pharmacological interventions for the prevention and treatment of amnesic shellfish poisoning and for other brain pathologies involving excitotoxicity.


Mar Drugs


Marine drugs


Gill SS,Hou Y,Ghane T,Pulido OM


Has Abstract


2008-02-14 00:00:00










  • Virescenosides From the Holothurian-Associated Fungus Acremonium Striatisporum Kmm 4401.

    abstract::Ten new diterpene glycosides virescenosides Z9-Z18 (1-10) together with three known analogues (11-13) and aglycon of virescenoside A (14) were isolated from the marine-derived fungus Acremonium striatisporum KMM 4401. These compounds were obtained by cultivating fungus on wort agar medium with the addition of potassiu...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Zhuravleva OI,Antonov AS,Oleinikova GK,Khudyakova YV,Popov RS,Denisenko VA,Pislyagin EA,Chingizova EA,Afiyatullov SS

    更新日期:2019-10-29 00:00:00

  • In vitro acylation of okadaic acid in the presence of various bivalves' extracts.

    abstract::The dinoflagellate Dinophysis spp. is responsible for diarrhetic shellfish poisoning (DSP). In the bivalves exposed to the toxic bloom of the dinoflagellate, dinophysistoxin 3 (DTX3), the 7-OH acylated form of either okadaic acid (OA) or DTX1, is produced. We demonstrated in vitro acylation of OA with palmitoyl CoA in...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Konoki K,Onoda T,Watanabe R,Cho Y,Kaga S,Suzuki T,Yotsu-Yamashita M

    更新日期:2013-01-29 00:00:00

  • Marine and semi-synthetic hydroxysteroids as new scaffolds for pregnane X receptor modulation.

    abstract::In recent years many sterols with unusual structures and promising biological profiles have been identified from marine sources. Here we report the isolation of a series of 24-alkylated-hydroxysteroids from the soft coral Sinularia kavarattiensis, acting as pregnane X receptor (PXR) modulators. Starting from this scaf...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Sepe V,Di Leva FS,D'Amore C,Festa C,De Marino S,Renga B,D'Auria MV,Novellino E,Limongelli V,D'Souza L,Majik M,Zampella A,Fiorucci S

    更新日期:2014-05-27 00:00:00

  • Fucoidan and Fucosylated Chondroitin Sulfate Stimulate Hematopoiesis in Cyclophosphamide-Induced Mice.

    abstract::Application of cytostatics in cancer patients' chemotherapy results in a number of side effects, including the inhibition of various parts of hematopoiesis. Two sulfated polysaccharides, fucoidan from the seaweed Chordaria flagelliformis (PS-Fuc) and fucosylated chondroitin sulfate from the sea cucumber Massinium magn...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Anisimova N,Ustyuzhanina N,Bilan M,Donenko F,Usov A,Kiselevskiy M,Nifantiev N

    更新日期:2017-09-30 00:00:00

  • Nutritional Profiling and the Value of Processing By-Products from Gilthead Sea Bream (Sparus aurata).

    abstract::Fish processing industries generate a large volume of discards. In order to fulfil with the principles of a sustainable circular economy, it is necessary to maintain aquaculture by-products in the food chain through the production of high-value biomolecules that can be used as novel ingredients. In this study, we try ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Pateiro M,Munekata PES,Domínguez R,Wang M,Barba FJ,Bermúdez R,Lorenzo JM

    更新日期:2020-02-04 00:00:00

  • Marine Compound 3-bromo-4,5-dihydroxybenzaldehyde Protects Skin Cells against Oxidative Damage via the Nrf2/HO-1 Pathway.

    abstract::In this study, we aimed to illustrate the potential bio-effects of 3-bromo-4,5-dihydroxybenzaldehyde (3-BDB) on the antioxidant/cytoprotective enzyme heme oxygenase-1 (HO-1) in keratinocytes. The antioxidant effects of 3-BDB were examined via reverse transcription PCR, Western blotting, HO-1 activity assay, and immuno...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Ryu YS,Fernando PDSM,Kang KA,Piao MJ,Zhen AX,Kang HK,Koh YS,Hyun JW

    更新日期:2019-04-19 00:00:00

  • Reclamation of Marine Chitinous Materials for the Production of α-Glucosidase Inhibitors via Microbial Conversion.

    abstract::Six kinds of chitinous materials have been used as sole carbon/nitrogen (C/N) sources for producing α-glucosidase inhibitors (aGI) by Paenibacillus sp. TKU042. The aGI productivity was found to be highest in the culture supernatants using demineralized crab shell powder (deCSP) and demineralized shrimp shell powder (d...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Nguyen VB,Wang SL

    更新日期:2017-11-07 00:00:00

  • Biotechnological Potential of Cold Adapted Pseudoalteromonas spp. Isolated from 'Deep Sea' Sponges.

    abstract::The marine genus Pseudoalteromonas is known for its versatile biotechnological potential with respect to the production of antimicrobials and enzymes of industrial interest. We have sequenced the genomes of three Pseudoalteromonas sp. strains isolated from different deep sea sponges on the Illumina MiSeq platform. The...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Borchert E,Knobloch S,Dwyer E,Flynn S,Jackson SA,Jóhannsson R,Marteinsson VT,O'Gara F,Dobson ADW

    更新日期:2017-06-19 00:00:00

  • Production, Characterization and Biocompatibility Evaluation of Collagen Membranes Derived from Marine Sponge Chondrosia reniformis Nardo, 1847.

    abstract::Collagen is involved in the formation of complex fibrillar networks, providing the structural integrity of tissues. Its low immunogenicity and mechanical properties make this molecule a biomaterial that is extremely suitable for tissue engineering and regenerative medicine (TERM) strategies in human health issues. Her...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Pozzolini M,Scarfì S,Gallus L,Castellano M,Vicini S,Cortese K,Gagliani MC,Bertolino M,Costa G,Giovine M

    更新日期:2018-03-29 00:00:00

  • Cyclic marinopyrrole derivatives as disruptors of Mcl-1 and Bcl-x(L) binding to Bim.

    abstract::A series of novel cyclic marinopyrroles were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-x(L), was evaluated using ELISA assays. Both atropisomers of marinopyrrole A (1) show similar potency. A tetrabromo congener 9 is t...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Cheng C,Liu Y,Balasis ME,Simmons NL,Li J,Song H,Pan L,Qin Y,Nicolaou KC,Sebti SM,Li R

    更新日期:2014-03-07 00:00:00

  • Bioactive peptides and depsipeptides with anticancer potential: sources from marine animals.

    abstract::Biologically active compounds with different modes of action, such as, antiproliferative, antioxidant, antimicrotubule, have been isolated from marine sources, specifically algae and cyanobacteria. Recently research has been focused on peptides from marine animal sources, since they have been found as secondary metabo...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Suarez-Jimenez GM,Burgos-Hernandez A,Ezquerra-Brauer JM

    更新日期:2012-05-01 00:00:00

  • Prospecting Biotechnologically-Relevant Monooxygenases from Cold Sediment Metagenomes: An In Silico Approach.

    abstract::The goal of this work was to identify sequences encoding monooxygenase biocatalysts with novel features by in silico mining an assembled metagenomic dataset of polar and subpolar marine sediments. The targeted enzyme sequences were Baeyer-Villiger and bacterial cytochrome P450 monooxygenases (CYP153). These enzymes ha...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Musumeci MA,Lozada M,Rial DV,Mac Cormack WP,Jansson JK,Sjöling S,Carroll J,Dionisi HM

    更新日期:2017-04-09 00:00:00

  • Astaxanthin Inhibits PC-3 Xenograft Prostate Tumor Growth in Nude Mice.

    abstract::Prostate cancer (PCa), the most common malignancy in men, is a major cause of cancer deaths. A better understanding of the mechanisms that drive tumor initiation and progression may identify actionable targets to improve treatment of this patient group. As a dietary carotenoid, astaxanthin has been demonstrated to exe...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Ni X,Yu H,Wang S,Zhang C,Shen S

    更新日期:2017-03-08 00:00:00

  • Bioactive Bis(indole) Alkaloids from a Spongosorites sp. Sponge.

    abstract::Six new bis(indole) alkaloids (1-6) along with eight known ones of the topsentin class were isolated from a Spongosorites sp. sponge of Korea. Based on the results of combined spectroscopic analyses, the structures of spongosoritins A-D (1-4) were determined to possess a 2-methoxy-1-imidazole-5-one core connecting the...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Park JS,Cho E,Hwang JY,Park SC,Chung B,Kwon OS,Sim CJ,Oh DC,Oh KB,Shin J

    更新日期:2020-12-23 00:00:00

  • Potential anti-HPV and related cancer agents from marine resources: an overview.

    abstract::Recently, the studies on the prevention and treatment of human papillomavirus (HPV) which is closely related to the cervical cancer and other genital diseases are attracting more and more attention all over the world. Marine-derived polysaccharides and other bioactive compounds have been shown to possess a variety of ...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Wang SX,Zhang XS,Guan HS,Wang W

    更新日期:2014-04-03 00:00:00

  • The Antimicrobial Properties of Chitosan Can be Tailored by Formulation.

    abstract::Topical administration of drugs into the vagina can provide local therapy of vaginal infections, preventing the possible systemic side effects of the drugs. The natural polysaccharide chitosan is known for its excellent mucoadhesive properties, safety profile, and antibacterial effects, and thus it can be utilized in ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Jøraholmen MW,Bhargava A,Julin K,Johannessen M,Škalko-Basnet N

    更新日期:2020-01-31 00:00:00

  • A Saponification Method for Chlorophyll Removal from Microalgae Biomass as Oil Feedstock.

    abstract::Microalgae oil is an optimal feedstock for nutraceutical, pharmaceutical and biodiesel production, but its high levels of chlorophyll limit its large-scale application. To date, few effective approaches have been developed to remove chlorophyll from microalgae oil. The main purpose of this study was to present a prepr...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Li T,Xu J,Wu H,Wang G,Dai S,Fan J,He H,Xiang W

    更新日期:2016-09-07 00:00:00

  • Antithrombotics from the Sea: Polysaccharides and Beyond.

    abstract::Marine organisms exhibit some advantages as a renewable source of potential drugs, far beyond chemotherapics. Particularly, the number of marine natural products with antithrombotic activity has increased in the last few years, and reports show a wide diversity in scaffolds, beyond the polysaccharide framework. While ...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Carvalhal F,Cristelo RR,Resende DISP,Pinto MMM,Sousa E,Correia-da-Silva M

    更新日期:2019-03-16 00:00:00

  • Comparative Analysis of Culture Conditions for the Optimization of Carotenoid Production in Several Strains of the Picoeukaryote Ostreococcus.

    abstract::Microalgae are promising sources for the sustainable production of compounds of interest for biotechnologies. Compared to higher plants, microalgae have a faster growth rate and can be grown in industrial photobioreactors. The microalgae biomass contains specific metabolites of high added value for biotechnology such ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Guyon JB,Vergé V,Schatt P,Lozano JC,Liennard M,Bouget FY

    更新日期:2018-02-28 00:00:00

  • Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice.

    abstract::Impaired wound healing is a major clinical problem in patients with diabetes and is the leading cause of lower limb amputation. This study is aimed to observe the effects of small molecule oligopeptides isolated from sea cucumber (SCCOPs) on the wound healing process in diabetic mice. Ninety db/db male mice were divid...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Li D,Li L,Xu T,Wang T,Ren J,Liu X,Li Y

    更新日期:2018-01-08 00:00:00

  • Fermentation technologies for the optimization of marine microbial exopolysaccharide production.

    abstract::In the last decades, research has focused on the capabilities of microbes to secrete exopolysaccharides (EPS), because these polymers differ from the commercial ones derived essentially from plants or algae in their numerous valuable qualities. These biopolymers have emerged as new polymeric materials with novel and u...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Finore I,Di Donato P,Mastascusa V,Nicolaus B,Poli A

    更新日期:2014-05-22 00:00:00

  • Collagen Extraction Optimization from the Skin of the Small-Spotted Catshark (S. canicula) by Response Surface Methodology.

    abstract::The small-spotted catshark is one of the most abundant elasmobranchs in the Northeastern Atlantic Ocean. Although its landings are devoted for human consumption, in general this species has low commercial value with high discard rates, reaching 100% in some European fisheries. The reduction of post-harvest losses (dis...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Blanco M,Vázquez JA,Pérez-Martín RI,G Sotelo C

    更新日期:2019-01-09 00:00:00

  • Marinopyrrole derivatives with sulfide spacers as selective disruptors of Mcl-1 binding to pro-apoptotic protein Bim.

    abstract::A series of novel marinopyrroles with sulfide and sulphone spacers were designed and synthesized. Their activity to disrupt the binding of the pro-apoptotic protein, Bim, to the pro-survival proteins, Mcl-1 and Bcl-xL, was evaluated using ELISA assays. Fluorescence-quenching (FQ) assays confirmed the direct binding of...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Cheng C,Liu Y,Balasis ME,Garner TP,Li J,Simmons NL,Berndt N,Song H,Pan L,Qin Y,Nicolaou KC,Gavathiotis E,Sebti SM,Li R

    更新日期:2014-07-29 00:00:00

  • Vasorelaxation, induced by Dictyota pulchella (Dictyotaceae), a brown alga, is mediated via inhibition of calcium influx in rats.

    abstract::This study aimed to investigate the cardiovascular effects elicited by Dictyota pulchella, a brown alga, using in vivo and in vitro approaches. In normotensive conscious rats, CH(2)Cl(2)/MeOH Extract (CME, 5, 10, 20 and 40 mg/kg) from Dictyota pulchella produced dose-dependent hypotension (-4 ± 1; -8 ± 2; -53 ± 8 and ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Queiroz TM,Machado NT,Furtado FF,Oliveira-Filho AA,Alustau MC,Figueiredo CS,Miranda GE,Barbosa-Filho JM,Braga VA,Medeiros IA

    更新日期:2011-01-01 00:00:00

  • Biosynthetic studies on water-soluble derivative 5c (DTX5c).

    abstract::The dinoflagellate Prorocentrum belizeanum is responsible for the production of several toxins involved in the red tide phenomenon known as Diarrhetic Shellfish Poisoning (DSP). In this paper we report on the biosynthetic origin of an okadaic acid water-soluble ester derivative, DTX5c, on the basis of the spectroscopi...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Vilches TS,Norte M,Daranas AH,Fernández JJ

    更新日期:2012-10-01 00:00:00

  • Pharmacokinetic Study of Bioactive Glycopeptide from Strongylocentrotus droebachiensis After Intranasal Administration to Rats Using Biomarker Approach.

    abstract::A glycopeptide fraction (GPF) from internal organs of green sea urchins (Strongylocentrotus droebachiensis Müller, Strongylocentrotidae) has been reported to be an effective bronchitis treatment. In this study, we evaluated the pharmacokinetic and tissue distribution of GPF, following single and repeated intranasal (i...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Shikov AN,Pozharitskaya ON,Faustova NM,Kosman VM,Makarov VG,Razzazi-Fazeli E,Novak J

    更新日期:2019-10-11 00:00:00

  • Seaweed hydrocolloid production: an update on enzyme assisted extraction and modification technologies.

    abstract::Agar, alginate, and carrageenans are high-value seaweed hydrocolloids, which are used as gelation and thickening agents in different food, pharmaceutical, and biotechnological applications. The annual global production of these hydrocolloids has recently reached 100,000 tons with a gross market value just above US$ 1....

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Rhein-Knudsen N,Ale MT,Meyer AS

    更新日期:2015-05-27 00:00:00

  • Marine myxobacteria as a source of antibiotics--comparison of physiology, polyketide-type genes and antibiotic production of three new isolates of Enhygromyxa salina.

    abstract::Three myxobacterial strains, designated SWB004, SWB005 and SWB006, were obtained from beach sand samples from the Pacific Ocean and the North Sea. The strains were cultivated in salt water containing media and subjected to studies to determine their taxonomic status, the presence of genes for the biosynthesis of polyk...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Schäberle TF,Goralski E,Neu E,Erol O,Hölzl G,Dörmann P,Bierbaum G,König GM

    更新日期:2010-09-03 00:00:00

  • Cephalopods as vectors of harmful algal bloom toxins in marine food webs.

    abstract::Here we summarize the current knowledge on the transfer and accumulation of harmful algal bloom (HAB)-related toxins in cephalopods (octopods, cuttlefishes and squids). These mollusks have been reported to accumulate several HAB-toxins, namely domoic acid (DA, and its isomers), saxitoxin (and its derivatives) and paly...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Lopes VM,Lopes AR,Costa P,Rosa R

    更新日期:2013-09-06 00:00:00

  • Biotechnological Production of Docosahexaenoic Acid Using Aurantiochytrium limacinum: Carbon Sources Comparison And Growth Characterization.

    abstract::Aurantiochytrium limacinum, a marine heterotrophic protist/microalga has shown interesting yields of docosahexaenoic acid (DHA) when cultured with different carbon sources: glucose, pure and crude glycerol. A complete study in a lab-scale fermenter allowed for the characterization and comparison of the growth kinetic ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Abad S,Turon X

    更新日期:2015-12-05 00:00:00