Characterization and cytotoxicity studies of the rare 21:4 n-7 acid and other polyunsaturated fatty acids from the marine opisthobranch Scaphander lignarius, isolated using bioassay guided fractionation.


:The marine opisthobranch Scaphander lignarius has been analyzed in the systematic search for novel bioactive compounds in Arctic marine organisms using bioassay guided fractionation. A number of highly cytotoxic fractions were shown to contain mainly polyunsaturated fatty acids (PUFAs). Selected PUFAs were isolated and identified using both liquid chromatography-mass spectrometry (LC-MS) and nuclear magnetic resonance (NMR). It was shown that the opisthobranch contained unusual PUFAs such as several ω3 fatty acids and the ω7 heneicosa-5,8,11,14-tetraenoic acid (21:4 n-7) not isolated before. The organism was shown to be a very rich source of PUFAs and the activity of the isolated compounds against a range of human cancer cell lines (melanoma, colon carcinoma and breast carcinoma) is further reported. The ω7 PUFA was significantly more cytotoxic in comparison with reference ω6 arachidonic and ω3 eicosapentaenoic acid. A noteworthy non-selective cytotoxicity against normal lung fibroblasts was also established. The paper contains isolation protocols in addition to cytotoxicity data of the isolated compounds. The potential of marine mollusks as a source for rare PUFAs is also discussed.


Mar Drugs


Marine drugs


Vasskog T,Andersen JH,Hansen E,Svenson J




Has Abstract


2012-12-01 00:00:00










  • Preparation of chitosan nanocomposites with a macroporous structure by unidirectional freezing and subsequent freeze-drying.

    abstract::Chitosan is the N-deacetylated derivative of chitin, a naturally abundant mucopolysaccharide that consists of 2-acetamido-2-deoxy-β-d-glucose through a β (1→4) linkage and is found in nature as the supporting material of crustaceans, insects, etc. Chitosan has been strongly recommended as a suitable functional materia...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Aranaz I,Gutiérrez MC,Ferrer ML,del Monte F

    更新日期:2014-11-24 00:00:00

  • Ciguatoxins Evoke Potent CGRP Release by Activation of Voltage-Gated Sodium Channel Subtypes NaV1.9, NaV1.7 and NaV1.1.

    abstract::Ciguatoxins (CTXs) are marine toxins that cause ciguatera fish poisoning, a debilitating disease dominated by sensory and neurological disturbances that include cold allodynia and various painful symptoms as well as long-lasting pruritus. Although CTXs are known as the most potent mammalian sodium channel activator to...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Touska F,Sattler S,Malsch P,Lewis RJ,Reeh PW,Zimmermann K

    更新日期:2017-08-30 00:00:00

  • Effect of Low Molecular Weight Oligopeptides Isolated from Sea Cucumber on Diabetic Wound Healing in db/db Mice.

    abstract::Impaired wound healing is a major clinical problem in patients with diabetes and is the leading cause of lower limb amputation. This study is aimed to observe the effects of small molecule oligopeptides isolated from sea cucumber (SCCOPs) on the wound healing process in diabetic mice. Ninety db/db male mice were divid...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Li D,Li L,Xu T,Wang T,Ren J,Liu X,Li Y

    更新日期:2018-01-08 00:00:00

  • Indole diterpenoids and isocoumarin from the fungus, Aspergillus flavus, isolated from the prawn, Penaeus vannamei.

    abstract::Two new indole-diterpenoids (1 and 2) and a new isocoumarin (3), along with the known β-aflatrem (4), paspalinine (5), leporin B (6), α-cyclopiazonic acid (7), iso-α-cyclopiazonic acid (8), ditryptophenaline (9), aflatoxin B1 (10), 7-O-acetylkojic acid (11) and kojic acid (12), were isolated from the fermentation brot...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Sun K,Li Y,Guo L,Wang Y,Liu P,Zhu W

    更新日期:2014-06-30 00:00:00

  • Absorption and Transport of Sea Cucumber Saponins from Apostichopus japonicus.

    abstract::The present study is focused on the intestinal absorption of sea cucumber saponins. We determined the pharmacokinetic characteristics and bioavailability of Echinoside A and Holotoxin A₁; the findings indicated that the bioavailability of Holotoxin A₁ was lower than Echinoside A. We inferred that the differences in ch...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Li S,Wang Y,Jiang T,Wang H,Yang S,Lv Z

    更新日期:2016-06-17 00:00:00

  • Synthesis of Alkyl-Glycerolipids Standards for Gas Chromatography Analysis: Application for Chimera and Shark Liver Oils.

    abstract::Natural O-alkyl-glycerolipids, also known as alkyl-ether-lipids (AEL), feature a long fatty alkyl chain linked to the glycerol unit by an ether bond. AEL are ubiquitously found in different tissues but, are abundant in shark liver oil, breast milk, red blood cells, blood plasma, and bone marrow. Only a few AEL are com...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Pinault M,Guimaraes C,Couthon H,Thibonnet J,Fontaine D,Chantôme A,Chevalier S,Besson P,Jaffrès PA,Vandier C

    更新日期:2018-03-23 00:00:00

  • From Marine Venoms to Drugs: Efficiently Supported by a Combination of Transcriptomics and Proteomics.

    abstract::The potential of marine natural products to become new drugs is vast; however, research is still in its infancy. The chemical and biological diversity of marine toxins is immeasurable and as such an extraordinary resource for the discovery of new drugs. With the rapid development of next-generation sequencing (NGS) an...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Xie B,Huang Y,Baumann K,Fry BG,Shi Q

    更新日期:2017-03-30 00:00:00

  • Design and Preclinical Evaluation of Chitosan/Kaolin Nanocomposites with Enhanced Hemostatic Efficiency.

    abstract::In the current study, hemostatic compositions including a combination of chitosan and kaolin have been developed. Chitosan is a marine polysaccharide derived from chitins, a structural component in the shells of crustaceans. Both chitosan and kaolin have the ability to mediate a quick and efficient hemostatic effect f...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Elsabahy M,Hamad MA

    更新日期:2021-01-22 00:00:00

  • Polyhydroxylated steroids from the South China Sea soft coral Sarcophyton sp. and their cytotoxic and antiviral activities.

    abstract::Chemical investigation on the soft coral Sarcophyton sp. collected from the South China Sea yielded three new polyhydroxylated steroids, compounds (1-3), together with seven known ones (4-10). Their structures were established by extensive spectroscopic methods and comparison of their data with those of the related kn...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Gong KK,Tang XL,Zhang G,Cheng CL,Zhang XW,Li PL,Li GQ

    更新日期:2013-12-02 00:00:00

  • Two New Piperazine-Triones from a Marine-Derived Streptomycetes sp. Strain SMS636.

    abstract::Two new piperazine-triones lansai E and F (1, 2), together with four known secondary metabolites lansai D (3), 1-N-methyl-(E,Z)-albonoursin (4), imidazo[4,5-e]-1,2,4-triazine (5), and streptonigrin (6) were isolated from a deep-sea-derived Streptomycetes sp. strain SMS636. The structures of the isolated compounds were...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Xu X,Han J,Lin R,Polyak SW,Song F

    更新日期:2019-03-21 00:00:00

  • Theonellamide G, a potent antifungal and cytotoxic bicyclic glycopeptide from the Red Sea marine sponge Theonella swinhoei.

    abstract::In our search for bioactive metabolites from marine organisms, we have investigated the polar fraction of the organic extract of the Red Sea sponge Theonella swinhoei. Successive chromatographic separations and final HPLC purification of the potent antifungal fraction afforded a new bicyclic glycopeptide, theonellamid...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Youssef DT,Shaala LA,Mohamed GA,Badr JM,Bamanie FH,Ibrahim SR

    更新日期:2014-04-01 00:00:00

  • A New Bioassay Platform Design for the Discovery of Small Molecules with Anticancer Immunotherapeutic Activity.

    abstract::Immunotherapy takes advantage of the immune system to prevent, control, and eliminate neoplastic cells. The research in the field has already led to major breakthroughs to treat cancer. In this work, we describe a platform that integrates in vitro bioassays to test the immune response and direct antitumor effects for ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Gallo C,Barra G,Saponaro M,Manzo E,Fioretto L,Ziaco M,Nuzzo G,d'Ippolito G,De Palma R,Fontana A

    更新日期:2020-11-29 00:00:00

  • Characterization of Properties and Transglycosylation Abilities of Recombinant α-Galactosidase from Cold-Adapted Marine Bacterium Pseudoalteromonas KMM 701 and Its C494N and D451A Mutants.

    abstract::A novel wild-type recombinant cold-active α-d-galactosidase (α-PsGal) from the cold-adapted marine bacterium Pseudoalteromonas sp. KMM 701, and its mutants D451A and C494N, were studied in terms of their structural, physicochemical, and catalytic properties. Homology models of the three-dimensional α-PsGal structure, ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Bakunina I,Slepchenko L,Anastyuk S,Isakov V,Likhatskaya G,Kim N,Tekutyeva L,Son O,Balabanova L

    更新日期:2018-09-24 00:00:00

  • The Marine-Derived Fungus Clonostachys rosea, Source of a Rare Conjugated 4-Me-6E,8E-hexadecadienoic Acid Reducing Viability of MCF-7 Breast Cancer Cells and Gene Expression of Lipogenic Enzymes.

    abstract::A marine-derived strain of Clonostachys rosea isolated from sediments of the river Loire estuary (France) was investigated for its high lipid production. The fungal strain was grown on six different culture media to explore lipid production changes. An original branched conjugated fatty acid, mainly present in triglyc...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Dias AC,Ruiz N,Couzinet-Mossion A,Bertrand S,Duflos M,Pouchus YF,Barnathan G,Nazih H,Wielgosz-Collin G

    更新日期:2015-08-06 00:00:00

  • λ-Carrageenan Suppresses Tomato Chlorotic Dwarf Viroid (TCDVd) Replication and Symptom Expression in Tomatoes.

    abstract::The effect of carrageenans on tomato chlorotic dwarf viroid (TCDVd) replication and symptom expression was studied. Three-week-old tomato plants were spray-treated with iota(ɩ)-, lambda(λ)-, and kappa(κ)-carrageenan at 1 g·L-1 and inoculated with TCDVd after 48 h. The λ-carrageenan significantly suppressed viroid symp...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Sangha JS,Kandasamy S,Khan W,Bahia NS,Singh RP,Critchley AT,Prithiviraj B

    更新日期:2015-05-08 00:00:00

  • Anaerobic Digestion of Laminaria japonica Waste from Industrial Production Residues in Laboratory- and Pilot-Scale.

    abstract::The cultivation of macroalgae to supply the biofuel, pharmaceutical or food industries generates a considerable amount of organic residue, which represents a potential substrate for biomethanation. Its use optimizes the total resource exploitation by the simultaneous disposal of waste biomaterials. In this study, we e...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Barbot YN,Thomsen C,Thomsen L,Benz R

    更新日期:2015-09-18 00:00:00

  • Aplisulfamines, new sulfoxide-containing metabolites from an aplidium tunicate: absolute stereochemistry at chiral sulfur and carbon atoms assigned through an original combination of spectroscopic and computational methods.

    abstract::Two new sulfoxide-containing metabolites, aplisulfamines A and B, have been isolated from an Aplidium sp. collected in the Bay of Naples. Their planar structure and geometry of a double bond were readily determined by using standard methods, mainly NMR spectroscopy. An original approach was used to assign the absolute...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Aiello A,Fattorusso E,Imperatore C,Luciano P,Menna M,Vitalone R

    更新日期:2012-01-01 00:00:00

  • Bromopyrrole alkaloids as lead compounds against protozoan parasites.

    abstract::In the present study, 13 bromopyrrole alkaloids, including the oroidin analogs hymenidin (2), dispacamide B (3) and dispacamide D (4), stevensine (5) and spongiacidin B (6), their derivatives lacking the imidazole ring bromoaldisin (7), longamide B (8) and longamide A (9), the dimeric oroidin derivatives sceptrin (10)...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Scala F,Fattorusso E,Menna M,Taglialatela-Scafati O,Tierney M,Kaiser M,Tasdemir D

    更新日期:2010-07-14 00:00:00

  • Anti-Inflammatory Polyoxygenated Steroids from the Soft Coral Lobophytum michaelae.

    abstract::Three new polyoxygenated steroids, michosterols A-C (1-3), and four known compounds (4-7) were isolated from the ethyl acetate (EtOAc) extract of the soft coral Lobophytum michaelae, collected off the coast of Taitung. The structures of the new compounds were elucidated on the basis of spectroscopic analyses and compa...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Huang CY,Tseng WR,Ahmed AF,Chiang PL,Tai CJ,Hwang TL,Dai CF,Sheu JH

    更新日期:2018-03-13 00:00:00

  • Blood-Brain Barrier Permeable Chitosan Oligosaccharides Interfere with β-Amyloid Aggregation and Alleviate β-Amyloid Protein Mediated Neurotoxicity and Neuroinflammation in a Dose- and Degree of Polymerization-Dependent Manner.

    abstract::It is proven that β-amyloid (Aβ) aggregates containing cross-β-sheet structures led to oxidative stress, neuroinflammation, and neuronal loss via multiple pathways. Therefore, reduction of Aβ neurotoxicity via inhibiting aggregation of Aβ or dissociating toxic Aβ aggregates into nontoxic forms might be effective thera...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Zhu L,Li R,Jiao S,Wei J,Yan Y,Wang ZA,Li J,Du Y

    更新日期:2020-09-25 00:00:00

  • Anti-Neuroinflammatory Agent, Restricticin B, from the Marine-Derived Fungus Penicillium janthinellum and Its Inhibitory Activity on the NO Production in BV-2 Microglia Cells.

    abstract::A new compound containing a triene, a tetrahydropyran ring and glycine ester functionalities, restricticin B (1), together with four known compounds (2-5) were obtained from the EtOAc extract of the marine-derived fungus Penicillium janthinellum. The planar structure of 1 was determined by detailed analyses of MS, 1D ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Choi BK,Jo SH,Choi DK,Trinh PTH,Lee HS,Cao VA,Van TTT,Shin HJ

    更新日期:2020-09-14 00:00:00

  • Azaspiracid shellfish poisoning: a review on the chemistry, ecology, and toxicology with an emphasis on human health impacts.

    abstract::Azaspiracids (AZA) are polyether marine toxins that accumulate in various shellfish species and have been associated with severe gastrointestinal human intoxications since 1995. This toxin class has since been reported from several countries, including Morocco and much of western Europe. A regulatory limit of 160 micr...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Twiner MJ,Rehmann N,Hess P,Doucette GJ

    更新日期:2008-05-07 00:00:00

  • Natural Products from Cyanobacteria: Focus on Beneficial Activities.

    abstract::Cyanobacteria are photosynthetic microorganisms that colonize diverse environments worldwide, ranging from ocean to freshwaters, soils, and extreme environments. Their adaptation capacities and the diversity of natural products that they synthesize, support cyanobacterial success in colonization of their respective ec...

    journal_title:Marine drugs

    pub_type: 杂志文章,评审


    authors: Demay J,Bernard C,Reinhardt A,Marie B

    更新日期:2019-05-30 00:00:00

  • Leucine aminopeptidase, beta-glucosidase and alkaline phosphatase activity rates and their significance in nutrient cycles in some coastal Mediterranean sites.

    abstract::In aquatic microbial ecology, knowledge of the processes involved in the turnover of organic matter is of utmost importance to understand ecosystem functioning. Microorganisms are major players in the cycling of nutrients (nitrogen, phosphorus) and carbon, thanks to their enzymatic activities (leucine aminopeptidase, ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Caruso G

    更新日期:2010-03-29 00:00:00

  • Vasorelaxation, induced by Dictyota pulchella (Dictyotaceae), a brown alga, is mediated via inhibition of calcium influx in rats.

    abstract::This study aimed to investigate the cardiovascular effects elicited by Dictyota pulchella, a brown alga, using in vivo and in vitro approaches. In normotensive conscious rats, CH(2)Cl(2)/MeOH Extract (CME, 5, 10, 20 and 40 mg/kg) from Dictyota pulchella produced dose-dependent hypotension (-4 ± 1; -8 ± 2; -53 ± 8 and ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Queiroz TM,Machado NT,Furtado FF,Oliveira-Filho AA,Alustau MC,Figueiredo CS,Miranda GE,Barbosa-Filho JM,Braga VA,Medeiros IA

    更新日期:2011-01-01 00:00:00

  • Cloning and characterization of glutamate receptors in Californian sea lions (Zalophus californianus).

    abstract::Domoic acid produced by marine algae has been shown to cause acute and chronic neurologic sequelae in Californian sea lions following acute or low-dose exposure. Histological findings in affected animals included a degenerative cardiomyopathy that was hypothesized to be caused by over-excitation of the glutamate recep...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Gill S,Goldstein T,Situ D,Zabka TS,Gulland FM,Mueller RW

    更新日期:2010-05-06 00:00:00

  • Virescenosides From the Holothurian-Associated Fungus Acremonium Striatisporum Kmm 4401.

    abstract::Ten new diterpene glycosides virescenosides Z9-Z18 (1-10) together with three known analogues (11-13) and aglycon of virescenoside A (14) were isolated from the marine-derived fungus Acremonium striatisporum KMM 4401. These compounds were obtained by cultivating fungus on wort agar medium with the addition of potassiu...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Zhuravleva OI,Antonov AS,Oleinikova GK,Khudyakova YV,Popov RS,Denisenko VA,Pislyagin EA,Chingizova EA,Afiyatullov SS

    更新日期:2019-10-29 00:00:00

  • New α-Glucosidase Inhibitory Triterpenic Acid from Marine Macro Green Alga Codium dwarkense Boergs.

    abstract::The marine ecosystem has been a key resource for secondary metabolites with promising biological roles. In the current study, bioassay-guided phytochemical investigations were carried out to assess the presence of enzyme inhibitory chemical constituents from the methanolic extract of marine green alga-Codium dwarkense...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Ali L,Khan AL,Al-Kharusi L,Hussain J,Al-Harrasi A

    更新日期:2015-07-14 00:00:00

  • Polysaccharide from Codium fragile Induces Anti-Cancer Immunity by Activating Natural Killer Cells.

    abstract::Natural polysaccharides exhibit beneficial immune modulatory effects, including immune stimulatory and anti-cancer activities. In this study, we examined the effect of Codium fragile polysaccharide (CFP) on natural killer (NK) cell activation, and its effect on tumor-bearing mice. Intravenous CFP treatment of C57BL/6 ...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Park HB,Hwang J,Zhang W,Go S,Kim J,Choi I,You S,Jin JO

    更新日期:2020-12-08 00:00:00

  • A kinetic study of accumulation and elimination of microcystin-LR in yellow perch (Perca flavescens) tissue and implications for human fish consumption.

    abstract::Fish consumption is a potential route of human exposure to the hepatotoxic microcystins, especially in lakes and reservoirs that routinely experience significant toxic Microcystis blooms. Understanding the rates of uptake and elimination for microcystins as well as the transfer efficiency into tissues of consumers are...

    journal_title:Marine drugs

    pub_type: 杂志文章


    authors: Dyble J,Gossiaux D,Landrum P,Kashian DR,Pothoven S

    更新日期:2011-12-01 00:00:00