Endogenous interleukin-22 protects against inflammatory bowel disease but not autoimmune cholangitis in dominant negative form of transforming growth factor beta receptor type II mice.


:During chronic inflammation, interleukin (IL)-22 expression is up-regulated in both CD4 and CD8 T cells, exerting a protective role in infections. However, in autoimmunity, IL-22 appears to have either a protective or a pathogenic role in a variety of murine models of autoimmunity and, by extrapolation, in humans. It is not clear whether IL-22 itself mediates inflammation or is a by-product of inflammation. We have taken advantage of the dominant negative form of transforming growth factor beta receptor type II (dnTGF-βRII) mice that develop both inflammatory bowel disease and autoimmune cholangitis and studied the role and the biological function of IL-22 by generating IL-22(-/-) dnTGF-βRII mice. Our data suggest that the influence of IL-22 on autoimmunity is determined in part by the local microenvironment. In particular, IL-22 deficiency exacerbates tissue injury in inflammatory bowel disease, but has no influence on either the hepatocytes or cholangiocytes in the same model. These data take on particular significance in the previously defined effects of IL-17A, IL-12p40 and IL-23p19 deficiency and emphasize that, in colitis, there is a dominant role of IL-23/T helper type 17 (Th17) signalling. Furthermore, the levels of IL-22 are IL-23-dependent. The use of cytokine therapy in patients with autoimmune disease has significant potential, but must take into account the overlapping and often promiscuous effects that can theoretically exacerbate inflammation.


Clin Exp Immunol


Yang GX,Sun Y,Tsuneyama K,Zhang W,Leung PS,He XS,Ansari AA,Bowlus C,Ridgway WM,Gershwin ME




Has Abstract


2016-08-01 00:00:00












  • The acquisition of anti-pneumococcal capsular polysaccharide Haemophilus influenzae type b and tetanus toxoid antibodies, with age, in the UK.

    abstract::Antibody levels specific for capsular polysaccharides of Streptococcus pneumoniae and Haemophilus influenzae type b (Hib) and to tetanus toxoid (TT), were measured in serum samples of 750 age-stratified subjects from the UK. The study subjects comprised healthy adult volunteers and hospitalized children undergoing ele...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hazlewood M,Nusrat R,Kumararatne DS,Goodall M,Raykundalia C,Wang DG,Joyce HJ,Milford-Ward A,Forte M,Pahor A

    更新日期:1993-08-01 00:00:00

  • Expression of anticardiolipin cofactor, human beta 2-glycoprotein I, by a recombinant baculovirus/insect cell system.

    abstract::A full-length cDNA coding a human beta 2-glycoprotein I (beta 2-GPI) was introduced into the baculovirus genome to construct a recombinant baculovirus. Spodoptera frugiperda (Sf9) cells were infected with the recombinant baculovirus. A protein (mol. wt 43,000) reactive with anti-beta 2-GPI antisera was produced in the...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Igarashi M,Matsuura E,Igarashi Y,Nagae H,Matsuura Y,Ichikawa K,Yasuda T,Voelker DR,Koike T

    更新日期:1993-07-01 00:00:00

  • Cytotoxic effect of autocrine and macrophage-derived nitric oxide on cultured rat mesangial cells.

    abstract::Expression of the inducible form of nitric oxide synthase (iNOS) has been found to be up-regulated in cytokine-stimulated mesangial cells (MC) and in experimental glomerulonephritis. Since direct toxicity of nitric oxide (NO) has been implicated in damage of bacteria, neoplastic and intact pancreatic cells, we investi...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hruby Z,Beck KF

    更新日期:1997-01-01 00:00:00

  • CD4+ T cells from HIV-1-infected patients recognize wild-type and mutant human immunodeficiency virus-1 protease epitopes.

    abstract::Human immunodeficiency virus (HIV)-1 protease is a known target of CD8+ T cell responses, but it is the only HIV-1 protein in which no fully characterized HIV-1 protease CD4 epitopes have been identified to date. We investigated the recognition of HIV-1 protease by CD4+ T cells from 75 HIV-1-infected, protease inhibit...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Muller NG,Alencar R,Jamal L,Hammer J,Sidney J,Sette A,Brindeiro RM,Kalil J,Cunha-Neto E,Moraes SL

    更新日期:2011-04-01 00:00:00

  • 5,7-dihydroxy-3,4,6-trimethoxyflavone inhibits the inflammatory effects induced by Bacteroides fragilis enterotoxin via dissociating the complex of heat shock protein 90 and I kappaB alpha and I kappaB kinase-gamma in intestinal epithelial cell culture.

    abstract::Enterotoxin produced by enterotoxigenic Bacteroides fragilis (BFT) has been associated with mucosal inflammation and diarrhoeal diseases. In this study, the anti-inflammatory molecular mechanism of 5,7-dihydroxy-3,4,6-trimethoxyflavone (eupatilin) was characterized in an HT-29 intestinal epithelial cell line stimulate...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kim JM,Lee DH,Kim JS,Lee JY,Park HG,Kim YJ,Oh YK,Jung HC,Kim SI

    更新日期:2009-03-01 00:00:00

  • HLA class II glycoprotein expression by monocytes from HIV-infected individuals: differences in response to interferon-gamma.

    abstract::The expression of HLA class II glycoproteins by monocytes from HIV-infected individuals was evaluated. We found no differences in HLA-DR, HLA-DQ, or HLA-DP expression by freshly isolated monocytes from patients with clinical AIDS, HIV-infected patients without AIDS or from uninfected individuals. However, monocytes fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Zwilling BS,Darrow R,Mcpeek M,Laufman H,Pearl D

    更新日期:1990-12-01 00:00:00

  • Effect of IgG for intravenous use on Fc receptor-mediated phagocytosis by human monocytes.

    abstract::Polyspecific IgG given intravenously at high doses (IVIG) is used for immunomodulatory therapy in autoimmune diseases such as idiopathic thrombocytopenic purpura and myasthenia gravis. It is assumed that the clinical effect is brought about in part by a modulation of mononuclear phagocyte function, in particular by an...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Jungi TW,Brcic M,Kuhnert P,Spycher MO,Li F,Nydegger UE

    更新日期:1990-10-01 00:00:00

  • Amyloid fibril protein AA in Papua New Guinean amyloidosis.

    abstract::In this study of protein composition of amyloid fibrils isolated from eight patients representative of the spectrum of amyloidosis found in Papua New Guinea has been investigated. All fibril preparations, including three from patients with amyloidosis secondary to lepromatous leprosy and one from an unusual juvenile c...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Anders RF,Price MA,Wilkey IS,Husby G,Takitaki F,Natvig JB,McAdam KP

    更新日期:1976-04-01 00:00:00

  • The micro-sperm immobilization test: the use of only motile spermatozoa and studies of complement.

    abstract::A technique is described which shows how to obtain a suspension of only progressively motile spermatozoa, from semen, by active penetration into an overlying buffer layer. About 60% of the number of motile spermatozoa in the original semen sample can be obtained as an output in the removed buffer layer. With this meth...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Hellema HW,Rümke P

    更新日期:1978-01-01 00:00:00

  • Failure of Mycobacterium leprae soluble antigens to suppress delayed-type hypersensitivity reaction to tuberculin.

    abstract::In order to test a published claim that the inclusion of Mycobacterium leprae antigens with a tuberculin skin test reagent can suppress delayed-type hypersensitivity (DTH) to tuberculin in both paucibacillary and multibacillary leprosy cases, 109 leprosy cases and 104 non-leprosy controls were skin-tested simultaneous...

    journal_title:Clinical and experimental immunology

    pub_type: 临床试验,杂志文章,随机对照试验


    authors: Fine PE,Gruer PJ,Maine N,Ponnighaus JM,Rees RJ,Stanford JL

    更新日期:1989-08-01 00:00:00

  • 21-hydroxylase autoantibodies are more prevalent in Turner syndrome but without an association to the autoimmune polyendocrine syndrome type I.

    abstract::Turner syndrome (TS) is associated with an increased frequency of autoimmunity. Frequently observed autoimmune diseases in TS are also seen in the autoimmune polyendocrine syndrome type I (APS I), of which Addison disease is a key component. An overlapping antibody profile between TS and APS I could be considered. The...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Berglund A,Cleemann L,Oftedal BE,Holm K,Husebye ES,Gravholt CH

    更新日期:2019-03-01 00:00:00

  • Dichotomy of the human T cell response to Leishmania antigens. II. Absent or Th2-like response to gp63 and Th1-like response to lipophosphoglycan-associated protein in cells from cured visceral leishmaniasis patients.

    abstract::The T cell response to different Leishmania donovani antigens was investigated using peripheral blood mononuclear cells (PBMC) from Kenyans cured of visceral leishmaniasis and non-exposed Danes. Crude promastigote and amastigote antigens both induced proliferation and interferon-gamma (IFN-gamma) production in PBMC fr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kurtzhals JA,Hey AS,Jardim A,Kemp M,Schaefer KU,Odera EO,Christensen CB,Githure JI,Olafson RW,Theander TG

    更新日期:1994-06-01 00:00:00

  • Cytokine mRNA expression in the liver of patients with primary biliary cirrhosis (PBC) and chronic hepatitis B (CHB).

    abstract::The expression of cytokine mRNA species was determined in liver biopsies from six normal subjects, 18 patients with PBC and 14 patients with hepatitis B e antigen (HBeAg)-positive CHB using a reverse transcriptase-polymerase chain reaction (RT-PCR) technique. cDNA, obtained by reverse transcription using oligo d(T) pr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Shindo M,Mullin GE,Braun-Elwert L,Bergasa NV,Jones EA,James SP

    更新日期:1996-08-01 00:00:00

  • Generation of monoclonal anti-dermatan sulphate antibodies cross-reacting to calf thymus DNA.

    abstract::Monoclonal anti-dermatan sulphate antibodies were produced with hybridization after immunizing BALB/c mice with dermatan sulphate (DS). These antibodies were tested for cross-reactivity to double-stranded DNA (dsDNA) and heat-denatured DNA (ssDNA). Five clones had binding activity to DS, dsDNA and ssDNA in enzyme-link...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ohnishi K,Sagawa A,Nakabayashi T,Ohmoto A,Megumi,Yoshikawa M,Watanabe I,Mukai M,Yasuda I,Nakagawa S

    更新日期:1989-01-01 00:00:00

  • Ultrastructural studies bearing on the mechanism of UVB-impaired induction of contact hypersensitivity to DNCB in man.

    abstract::In both murine and human experimental systems, acute, low dose exposure of skin to ultraviolet B light (UVB) impairs the induction of allergic contact dermatitis (ACD) by haptens such as dinitrochlorobenzene (DNCB) in a significant proportion of individuals. By light microscopy, epidermal Langerhans cells (LC) have be...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mommaas AM,Mulder AA,Vermeer M,Boom BW,Tseng C,Taylor JR,Streilein JW

    更新日期:1993-06-01 00:00:00

  • Demonstration of increased anti-mycobacterial activity in peripheral blood monocytes after BCG vaccination in British school children.

    abstract::A blood sample was taken from children aged 13-15 years immediately before BCG vaccination and 8 weeks after. The children were tuberculin skin-test negative to PPD-S before vaccination and positive after. Mononuclear cells were separated from the blood, infected with Mycobacterium microti at a low bacterium/monocyte ...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cheng SH,Walker L,Poole J,Aber VR,Walker KB,Mitchison DA,Lowrie DB

    更新日期:1988-10-01 00:00:00

  • Determination of the cytokine profile in American cutaneous leishmaniasis using the polymerase chain reaction.

    abstract::The lymphokine profiles were determined in the skin lesions of the three distinct clinical forms of American cutaneous leishmaniasis (ACL), using a reverse transcriptase polymerase chain reaction (RT-PCR) and primers for various lymphokines. The message for interferon-gamma (IFN-gamma), tumour necrosis factor-beta (TN...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Cáceres-Dittmar G,Tapia FJ,Sánchez MA,Yamamura M,Uyemura K,Modlin RL,Bloom BR,Convit J

    更新日期:1993-03-01 00:00:00

  • An efficient one-step method for isolating immune complexes from whole serum using a monoclonal anti-C3g affinity immunosorbent.

    abstract::A simple and efficient method of extracting complement-fixing immune complexes (IC) from whole serum and recovering them has been developed. 125I-labelled, in vitro prepared IC in whole serum were incubated with Sepharose 4B covalently linked to monoclonal anti-C3g or anti-C1q and the binding and recovery of IC was mo...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Samuel DJ,Amlot PL,Shepherd P,Lachmann PJ

    更新日期:1986-08-01 00:00:00

  • Blocking lymphotoxin-beta receptor activation diminishes inflammation via reduced mucosal addressin cell adhesion molecule-1 (MAdCAM-1) expression and leucocyte margination in chronic DSS-induced colitis.

    abstract::The lymphotoxin-beta receptor (LTbetaR) pathway is critical for maintenance of organized lymphoid structures and is involved in the development of colitis. To investigate the mechanisms by which LTbetaR activation contributes to the pathology of chronic inflammation we used a soluble LTbetaR-Ig fusion protein as a com...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Stopfer P,Obermeier F,Dunger N,Falk W,Farkas S,Janotta M,Möller A,Männel DN,Hehlgans T

    更新日期:2004-04-01 00:00:00

  • The influence of age and gender on serum dehydroepiandrosterone sulphate (DHEA-S), IL-6, IL-6 soluble receptor (IL-6 sR) and transforming growth factor beta 1 (TGF-beta1) levels in normal healthy blood donors.

    abstract::Dysregulation of IL-6 synthesis is thought to play a role in the development of a number of age-related conditions, such as rheumatoid arthritis, osteoporosis, atherosclerosis, Alzheimer's disease and B cell malignancies. Recently it has been suggested that the production of IL-6 is influenced by the adrenal hormone d...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Young DG,Skibinski G,Mason JI,James K

    更新日期:1999-09-01 00:00:00

  • Intravenous immunoglobulin (IVIG) preparations induce apoptosis in TNF-alpha-stimulated endothelial cells via a mitochondria-dependent pathway.

    abstract::Endothelial cells (ECs) are a target in inflammation, and the death of EC is regulated by various factors. Although intravenous immunoglobulin (IVIG) preparations are known to be beneficial therapeutic agents for the treatment of autoimmune diseases and systemic inflammatory disorders, their mechanism of action have n...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Nakatani K,Takeshita S,Tsujimoto H,Sekine I

    更新日期:2002-03-01 00:00:00

  • Monocytes show immunoregulatory capacity on CD4+ T cells in a human in-vitro model of extracorporeal photopheresis.

    abstract::Extracorporeal photopheresis (ECP) is a widely used immunomodulatory therapy for the treatment of various T cell-mediated disorders such as cutaneous T cell lymphoma (CTCL), graft-versus-host disease (GvHD) or systemic sclerosis. Although clinical benefits of ECP are already well described, the underlying mechanism of...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wiese F,Reinhardt-Heller K,Volz M,Gille C,Köstlin N,Billing H,Handgretinger R,Holzer U

    更新日期:2019-03-01 00:00:00

  • Possible different mechanisms of B cell activation in systemic lupus erythematosus and rheumatoid arthritis: opposite expression of low-affinity receptors for IgE (CD23) on their peripheral B cells.

    abstract::To clarify the differential state of B cell activation in patients with systemic lupus erythematosus (SLE) and rheumatoid arthritis (RA), we investigated the expression of low-affinity receptor for IgE (Fc epsilon RII; CD23) on their peripheral B cells by a cytofluorometry using H107 (CD23) and Leu-16 (CD20) monoclona...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Kumagai S,Ishida H,Iwai K,Tsubata T,Umehara H,Ozaki S,Suginoshita T,Araya S,Imura H

    更新日期:1989-12-01 00:00:00

  • Antibody penetration of viable human cells. I. Increased penetration of human lymphocytes by anti-RNP IgG.

    abstract::Antibody penetration of viable cells and interaction with intracellular antigens may have major consequences for immunopathological processes in connective tissue diseases. We have reported previously that antibody can penetrate viable human lymphocytes. To assess further the role of antinuclear antibodies in this pro...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Ma J,Chapman GV,Chen SL,Melick G,Penny R,Breit SN

    更新日期:1991-04-01 00:00:00

  • IgG subclass distribution of antibody responses to protein and polysaccharide mycobacterial antigens in leprosy and tuberculosis patients.

    abstract::Immunoenzymatic assays were developed for the measurement of antibodies against mycobacterial lipoarabinomannan (LAM), a cell-free proteic extract (CFX) of Mycobacterium leprae, and the 38-kD protein antigen of M. tuberculosis. Sera from 108 leprosy patients, belonging to all clinical-immunological forms of the spectr...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Sousa AO,Henry S,Marója FM,Lee FK,Brum L,Singh M,Lagrange PH,Aucouturier P

    更新日期:1998-01-01 00:00:00

  • Neutrophil phagocytosis and killing in insulin-dependent diabetes.

    abstract::Neutrophil phagocytosis and killing of Candida albicans were examined using a radiometric assay in 25 patients with insulin-dependent diabetes and 17 controls under various in vitro metabolic conditions. Glucose was present at 5, 10 and 50 mM, beta-hydroxybutyrate at 1, 5 and 20 mM and glucose with beta-hydroxybutyrat...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Wilson RM,Reeves WG

    更新日期:1986-02-01 00:00:00

  • Antibodies from patients with psoriasis recognize N-acetylglucosamine terminals in glycoproteins from Pityrosporum ovale.

    abstract::We have previously reported the finding of circulating antibodies recognizing two proteins of 100 and 120 kD (PO100 and PO120) from Pityrosporum ovale in patients with psoriasis. These antibodies were specific, since they were not detected in normal sera nor in other diseases linked to P. ovale such as seborrhoeic der...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Mathov I,Plotkin L,Abatangelo C,Galimberti R,Squiquera L,Leoni J

    更新日期:1996-07-01 00:00:00

  • Altered CD46-mediated T cell co-stimulation in haemodialysis patients.

    abstract::While most of our understanding of immune dysfunction in dialysis patients involves alterations in CD28-CD80/86 signalling, nothing is known of CD46-mediated co-stimulation of T cells in these patients. Because C3b/C4b bind to CD46 and complement activation occurs during haemodialysis (HD), we addressed whether CD46-m...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Brinkkoetter PT,Marinaki S,Gottmann U,Fleckenstein S,Stump C,Van Der Woude FJ,Braun C,Yard BA

    更新日期:2005-03-01 00:00:00

  • IL-2 production by intestinal lamina propria cells in normal inflamed and cancer-bearing colons.

    abstract::Biologically significant levels of IL-2 activity were produced by isolated lamina propria mononuclear cells (LPMC) from normal intestine (n = 12), cancer-bearing colons (n = 35) and inflammatory bowel disease (IBD) affected tissue (n = 12). The levels of IL-2 produced were similar for all three sources of LPMC (normal...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Pullman WE,Doe WF

    更新日期:1992-04-01 00:00:00

  • High prevalence of anti-cardiolipin and other autoantibodies in a healthy elderly population.

    abstract::Serum samples from 64 apparently healthy individuals (32 men and 32 women, mean age 81.0 years) were examined for the prevalence of several autoantibodies, including rheumatoid factor (RF), antinuclear antibodies (ANA), antibodies to extracted cellular antigens Ro (SSA), La (SSB), Sm, U1nRNP and Scl-70. IgG and IgM is...

    journal_title:Clinical and experimental immunology

    pub_type: 杂志文章


    authors: Manoussakis MN,Tzioufas AG,Silis MP,Pange PJ,Goudevenos J,Moutsopoulos HM

    更新日期:1987-09-01 00:00:00