Abstract:
:T cells play a pivotal role in controlling the immune response and have been the focus of extensive research. We studied the process of in vitro generation of antigen-specific T effector cells (Teffs) to assess the dynamics of antigen presentation and determine the best conditions for cell therapy. We used a peptidic construct consisting of combined HLA class I and II epitopes of the tumor antigen MAGE-3 as an antigen. Monocytes were isolated from healthy donors and were differentiated to dendritic cells (DCs) in vitro. The peptide was added to the DC culture, the pulsed cells were transferred to a co-culture with lymphocytes from the same donor, either as irradiated feeders or untreated, and were cultured in the presence or absence of IL-2. Several rounds of restimulation followed. The cells were analyzed by Flow Cytometry, and cytokine levels were measured by ELISA and Cytometric Bead Array for Th1/Th2/Th17 profiling. The results showed that the lymphocytes in culture upregulated their activation markers and produced Th1 proinflammatory cytokines in response to the peptide, optimally when it was presented by non-irradiated dendritic cells in the presence of IL-2. In contrast, DC irradiation resulted in low activation potential and a shift toward a suppressive phenotype. After prolonged antigenic stimulation, the culture displayed Th17 polarization. In conclusion, the functional integrity of DCs is necessary for the development of antigen-specific Teffs, and culture conditions can be developed to create Teffs with specific properties for eventual use in cell therapy applications.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
de Lastic AL,Rodi M,Mouzaki Adoi
10.1016/j.imbio.2016.04.001subject
Has Abstractpub_date
2016-08-01 00:00:00pages
862-70issue
8eissn
0171-2985issn
1878-3279pii
S0171-2985(16)30052-3journal_volume
221pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::CD163 and CD91 are scavenging receptors with highly increased expression during the differentiation of monocytes into the anti-inflammatory macrophage phenotype. In addition, CD91 is expressed in monocyte-derived dendritic cells (MoDCs), where the receptor is suggested to be important for internalization of CD91-targe...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2006.05.019
更新日期:2006-01-01 00:00:00
abstract::Vitamin D [25(OH)D3] deficiency has been associated with asthma as in many inflammatory and autoimmune pathologies; however, there is still a lack of data about the effects of administration of vitamin D in immune regulation in young asthmatic patients. In this study, we investigated its inhibitory effect on the immun...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.07.009
更新日期:2014-11-01 00:00:00
abstract::The macrophage population of the gastro-intestinal tract of the rat was studied by using a set of three monoclonal antibodies (ED1, ED2 and ED3) directed against rat macrophages, an anti-Ia monoclonal antibody and by performing an acid phosphatase reaction. The lamina propria of the whole gastro-intestinal tract compr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(86)80053-5
更新日期:1986-08-01 00:00:00
abstract::Peritoneal as well as peripheral blood, but not spleen macrophages inhibit natural killer (NK) cell activity directed against adherent growing target cells. Inhibition was observed with macrophages (Mø): spleen cell ratios as low as 0.01:1. It was independent from the activation status of Mø and was not MHC restricted...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80063-6
更新日期:1982-12-01 00:00:00
abstract::We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety l...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80181-6
更新日期:1991-09-01 00:00:00
abstract::The concept of using mesenchymal stem cells (MSCs) in bone repair has progressively evolved, and the goal of cell-mediated therapy is to prolong the natural physiological abilities of healing, or substitute them, when these are lacking, failing, or progressing too slowly. The future application of MSCs in human therap...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2008.07.030
更新日期:2009-01-01 00:00:00
abstract::A case of a 35-year-old female with multiple sclerosis is reported who developed without apparent prior sensitization a lymphocytotoxic antibody with anti-HLA-B8 specificity. The antibody persisted for several years with the same titer. The cytotoxic activity of the patient's serum was contained within the IgM fractio...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80002-8
更新日期:1982-02-01 00:00:00
abstract::To recognize patients with inhibitory and neutralizing auto-antibodies to interferon-γ (AutoAbs-IFN-γ) presenting with the sporadic phenotype of Mendelian Susceptibility to Mycobacterial Disease (MSMD) mainly characterized by recurrent intracellular mycobacterium or/and salmonella infections, we comprehensively invest...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.08.281
更新日期:2013-05-01 00:00:00
abstract::Regulation of an immune response requires complex crosstalk between cells of the innate and adaptive immune systems, via both cell-cell contact and secretion of cytokines. An important cytokine with a broad regulatory role in the immune system is transforming growth factor-β (TGF-β). TGF-β is produced by and has effec...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2012.06.009
更新日期:2012-12-01 00:00:00
abstract::Antibody-mediated elimination of foreign antigens contributes to immune protection from viral infection. We have generated phycoerythrin-conjugated HIV-1 Gag/p24 and influenza virus nucleoprotein tetramers for flow cytometric analysis of blood cell populations involved in antibody-mediated immunity. We show that in th...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2009.02.004
更新日期:2010-03-01 00:00:00
abstract::Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the pro...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.04.009
更新日期:2011-10-01 00:00:00
abstract::Mycoplasma arthritidis produces a so-far only partially characterized soluble material (MAS) that has a potent mitogenic effect on T lymphocytes of several species. Similar to staphylococcal enterotoxins and a number of related toxins secreted by other species of bacteria, nanogram quantities of these so-called supera...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80254-8
更新日期:1992-11-01 00:00:00
abstract::Psoriatic inflammation has been shown to be associated with cardiovascular dysfunction and systemic inflammation. Recently, psoriasis has also been linked to hepatic disorders, however underlying mechanism connecting the two are unknown. IL-17A being a central pro-inflammatory cytokine in the pathogenesis of psoriasis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2016.10.013
更新日期:2017-02-01 00:00:00
abstract:BACKGROUND:Ficolins serve as soluble recognition molecules in the lectin pathway of complement. They are known to participate in the systemic host-response to infection but their role in local pulmonary defence is still incompletely understood. The purpose of this study was to clarify whether acute lung and systemic in...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.11.015
更新日期:2016-05-01 00:00:00
abstract::When sheep red blood cells (SRBC) were injected intraperitoneally (i.p.), intravenously (i.v.), or into the Peyer's patches, definite plaque-forming cell (PFC) and rosette-forming cell (RFC) responses were induced in the spleen but not in the mesenteric lymph nodes (MLN). When SRBC were injected directly into the sple...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(82)80047-8
更新日期:1982-05-01 00:00:00
abstract::Macrophage functions in the immune response depend on their ability to infiltrate tissues and organs. The penetration between and within the tissues requires degradation of extracellular matrix (ECM), a function performed by the specialized, endopeptidase- and actin filament- rich organelles located at the ventral sur...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.07.009
更新日期:2018-11-01 00:00:00
abstract::The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+/CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220med/CD43+ ce...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(00)80071-6
更新日期:2000-04-01 00:00:00
abstract::In the last decade new glucocorticoid (GC)-signalling mechanisms have emerged. The evolving field of non-genomic GC actions was precipitated from two major directions: (i) some rapid/acute clinical GC applications could not be explained based on the relatively slowly appearing genomic GC action and (ii) accumulating e...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2009.10.003
更新日期:2010-07-01 00:00:00
abstract::We investigated the mRNA expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) in human T cell lymphotropic virus type I (HTLV-I) p40tax-transfected U937 cells, a human monoblast cell line. Transfection of HTLV-I p40tax U937 cells induced up-regulation of iNOS mRNA expression and...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(97)80083-6
更新日期:1997-11-01 00:00:00
abstract::Activity of acid phosphatase was found to be higher in T than in non-T cells of human peripheral blood lymphocytes. The T cell mitogen PHA induced an increase in the magnitude of acid phosphatase activity, while the B cell mitogen LPS was not able to produce any effect on acid phosphatase activity. Acid phosphatase ac...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(85)80033-4
更新日期:1985-03-01 00:00:00
abstract::Chitin and β-glucan are conserved throughout evolution in the fungal cell wall and are the most common polysaccharides in fungal species. Together, these two polysaccharides form a structural scaffold that is essential for the survival of the fungus. In the present study, we demonstrated that Aspergillus fumigatus alk...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.10.003
更新日期:2014-03-01 00:00:00
abstract::Alzheimer's disease (AD) is the most common neurodegenerative disease and it poses an ever-increasing burden to an aging population. Several loci responsible for the rare, autosomal dominant form of AD have been identified (APP, PS1 and PS2), and these have facilitated the development of the amyloid cascade hypothesis...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2011.07.017
更新日期:2012-02-01 00:00:00
abstract::Peptide immunotherapy (PIT) represents a safe and efficacious therapeutic modality for allergic diseases. Present study evaluates immunotherapeutic potential of T cell peptides of major cockroach allergen, Per a 10 in murine model of airway allergy. Treatment with peptides T-P8 and T-P10 demonstrated maximal resolutio...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.01.003
更新日期:2019-05-01 00:00:00
abstract::"Brucelline INRA" is an aqueous extract of a rough strain of Brucella melitensis that is used to test cutaneous delayed hypersensitivity in man and animals. The cellular response of mice (either normal, or sensitized by a previous brucella infection) was investigated with local implanted "cell-traps" and by immunologi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(89)80003-8
更新日期:1989-03-01 00:00:00
abstract::The anticonvulsant drug phenytoin, in less than cytotoxic concentrations, caused significant reductions in Ig secretion by unstimulated or EBV-stimulated normal MNC, as measured by PFC or secretion of Ig into the culture medium. Isotype-specific LBL varied in their sensitivity, the secretion of IgA (1 line) and IgG (3...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(85)80094-2
更新日期:1985-09-01 00:00:00
abstract::Cell walls from Streptococcus pyogenes strain Sv (Group A, M type 3) were lysed with M1 endo-N-acetylmuramidase, and the group A-specific carbohydrate antigen was purified by Sephadex G-100 gel filtration. The initial eluting antigen (M1gA) peak was assessed for mitogenic and polyclonal lymphocyte-activating propertie...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(85)80078-4
更新日期:1985-11-01 00:00:00
abstract:BACKGROUND:Male reproductive tract infection and inflammation are important aetiological factors of infertility. Experimental Autoimmune Orchitis (EAO) is a model of chronic inflammation useful to study mechanisms of inflammatory reactions leading to testicular impairment. EAO is characterised by interstitial cell infi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.04.007
更新日期:2012-08-01 00:00:00
abstract::Paracoccidioides brasiliensis is a dimorphic fungus from the Paracoccidioides genus, which is the causative agent of paracoccidioidomycosis, a chronic, subacute or acute mycosis, with visceral and cutaneous involvement. This disease that is acquired through inhalation primarily attacks the lungs but, can spread to oth...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.09.003
更新日期:2016-02-01 00:00:00
abstract::Kinetic methods can provide significant information concerning the mechanism of cellular cytotoxicity reactions. Previous kinetic studies of cytotoxic T lymphocytes (Tc) have been hampered by the heterogeneity of the effector cell population tested. We therefore examined the kinetics of lysis mediated by cloned, IL 2 ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(88)80065-2
更新日期:1988-12-01 00:00:00
abstract::This study assessed variation in plasma levels of the complement regulatorC1 inhibitor (C1inh) in patients with age related macular degeneration (AMD) and controls. Plasma from391 AMD cases and 370 controls was assayed by rate nephelometry to determine C1inh protein levels. Protein levels were analysed for relationshi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.07.015
更新日期:2012-02-01 00:00:00