Abstract:
:The role of the thymic microenvironment in the development of murine thymic B cells has yet to be fully clarified. We therefore investigate the microenvironment that supports the development of mature thymic B cells (sIg+/B220+/CD43-B cells) from thymic B cell progenitors with immunophenotypes of sIg-/B220med/CD43+ cells. As we have previously reported, thymic B cells generated from these progenitors in the thymus are CD5+ B cells. We next study the in vitro condition that supports the differentiation of thymic B cell progenitors. Stromal cells (from the bone marrow or thymus), thymus-derived cell lines with the character of thymic nurse cells (TNCs) or thymic epithelial cells (TECs), or the bone marrow-derived cell line (MS-5) are tested for their ability to support B-lymphopoiesis from thymic B cell progenitors. Interestingly, thymic stromal cells (but neither stromal cells from the bone marrow nor stromal cell lines) support the differentiation of thymic B cell progenitors into thymic B cells in the presence of IL-7. Cortical epithelia (but not medullary epithelia, thymic macrophages or dendritic cells) are found to contribute to thymic B cell differentiation. Surface phenotype and Ig rearrangement analyses reveal that mature B cells generated in this condition are primarily CD5+ B cells, indicating that the thymic microenvironment (particularly cortical epithelia) determines the differentiation of thymic B cells.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Sugihara A,Inaba M,Mori SI,Taketani S,Adachi Y,Hisha H,Inaba K,Toki J,Horio T,Gershwin ME,Ikehara Sdoi
10.1016/S0171-2985(00)80071-6subject
Has Abstractpub_date
2000-04-01 00:00:00pages
515-26issue
5eissn
0171-2985issn
1878-3279pii
S0171-2985(00)80071-6journal_volume
201pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::Ficolins are a group of proteins containing both a collagen-like domain and a fibrinogen-like domain and are found in varying tissues. Ficolins present in sera have a lectin activity toward N-acetylglucosamine through their fibrinogen-like domains. The domain organizations between ficolins and mannose-binding lectin (...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1078/0171-2985-00149
更新日期:2002-09-01 00:00:00
abstract::Macrophages located in airways and the alveolar space are continually exposed to different signals from the respiratory mucosa. In this respect, epithelial cells represent an important source of cytokines and mediators modulating the state of activation and/or differentiation of mononuclear phagocytes. Many of the pro...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2011.04.009
更新日期:2011-10-01 00:00:00
abstract::We studied immunohistological and biochemical aspects of the CD44 molecule with a mAb produced in our lab: GRHL-1. The characteristic expression of this antigen in cells of B lineage was analyzed. This mAb showed identical immunohistological patterns of reactivity to other mAbs included in CD44 cluster, on a variety l...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80181-6
更新日期:1991-09-01 00:00:00
abstract::Shigella spp. are known to penetrate the colonic epithelial cells causing shigellosis, which results in production of convalescent antibodies against porin, the surface exposed major outer membrane protein. Porin has been categorized as primarily TLR2-ligand and here we validated its signaling procedure in colonic INT...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.12.002
更新日期:2014-05-01 00:00:00
abstract::Interleukin-7 (IL-7) supports the proliferation of mature T lymphocytes, however, the cellular source of IL-7 for T lymphocyte activation has not been well established. We therefore investigated whether human peripheral blood dendritic cells (DC) produce IL-7 as a contribution towards T lymphocyte activation. Human CM...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(98)80075-2
更新日期:1998-03-01 00:00:00
abstract::When monocytes are stimulated with LPS (lipopolysaccharide) repeatedly then the initially high expression of the TNF (tumor necrosis factor) gene is only very low, i.e. the cells are tolerant to LPS. Tolerant cells still express the CD14 receptor and they can still be activated to mobilize NF-kappa B into nucleus. Ana...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(97)80029-0
更新日期:1997-12-01 00:00:00
abstract::Canine visceral leishmaniasis (CVL) is known to affect the cellular immunity of infected dogs, through impairing lymphoproliferation and microbicidal mechanisms. This study examined heme oxygenase-1 (HO-1) and its metabolites, oxidative stress and IL-10 levels in CVL and investigated correlations between these paramet...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2016.12.006
更新日期:2017-05-01 00:00:00
abstract::Individuals infected with Strongyloides stercoralis have been reported to produce different immunoglobulins isotypes, yet few studies have evaluated their use in strongyloidiasis diagnosis. The aim of this work was to evaluate the immunoreactivity of different classes and subclasses of anti-S. stercoralis circulating ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.151898
更新日期:2020-03-01 00:00:00
abstract::The aryl hydrocarbon receptor (AhR) is an environmental sensor and ligand-activated transcription factor that is critically involved in the regulation of inflammatory responses and the induction of tolerance by modulating immune cells. As dendritic cells (DCs) express high AhR levels, they are efficient to induce immu...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.151929
更新日期:2020-07-01 00:00:00
abstract::Culture supernatants (CS) from Hodgkin derived cell lines have previously been shown to contain colony stimulating activity (CSF) for human cord blood cells, fetal bone marrow and fetal liver cells. In this study 3-day CS from four Hodgkin lines (L428, L538, L540, L591) and two sublines (L428KS, L428KSA) were examined...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(84)80049-2
更新日期:1984-05-01 00:00:00
abstract::Macrophages are major effector cells of the innate immune system, and appropriate regulation of macrophage function requires the integration of multiple signalling inputs derived from the recognition of host factors (e.g. interferon-gamma/IFNgamma) and pathogen products (e.g. toll-like receptor/TLR agonists). The prof...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2006.05.007
更新日期:2006-01-01 00:00:00
abstract::Cytokines have profound effects on cells, and act through receptors which need only be at low concentrations (around 100 copies per cell) to transmit activation signals. The detection of such low concentrations is possible using monoclonal antibodies and fluorescence/flow cytometry, but only by using specialized techn...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/S0171-2985(11)80652-2
更新日期:1992-08-01 00:00:00
abstract::Ribosomal 30S and 50S subunits as well as total proteins from the large subunit of E. coli ribosomes were found to be potent mitogens for both human adult and cord blood lymphocytes. On the other hand, the RNA fraction of the 50S subunit had only a weak stimulatory capacity suggesting that the protein moiety contains ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(80)80079-9
更新日期:1980-01-01 00:00:00
abstract::Considering that imbalance of central tolerance in the thymus contributes to aggressive autoimmunity, we compared the expression of peripheral tissue autoantigens (PTA) genes, which are involved in self-representation in the thymic stroma, of two mouse strains; DBA-1/J (MHC-H2(q)) susceptible and DBA-2/J (MHC-H2(d)) r...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.007
更新日期:2011-05-01 00:00:00
abstract::LIF activity production by ConA-stimulated mononuclear cells was tested by measuring granulocyte migration from clotted plasma droplets placed in Microtest II plates. 90 min. incubation of MNC cultures with ConA assured a significant LIF activity in the 24 h. culture supernatants. Removal of the adherent cells after C...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(81)80097-6
更新日期:1981-01-01 00:00:00
abstract::Acute Myeloid Leukaemia (AML) is a neoplasia characterised by rapid proliferation and an increased rate of relapses. The AML blasts display features of antigen-presenting cells (APC), and thus can directly modulate the anti-tumour T cell responses. The bone marrow of a group consisting of 30 newly diagnosed patients a...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.152049
更新日期:2020-12-03 00:00:00
abstract::The alarming rise of obesity and type 2 diabetes (T2D) has put a tremendous strain on global healthcare systems. Over the past decade extensive research has focused on the role of macrophages as key mediators of inflammation in T2D. The inflammatory environment in the obese adipose tissue and pancreatic β-cell islets ...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2016.08.011
更新日期:2017-10-01 00:00:00
abstract::A case of a 35-year-old female with multiple sclerosis is reported who developed without apparent prior sensitization a lymphocytotoxic antibody with anti-HLA-B8 specificity. The antibody persisted for several years with the same titer. The cytotoxic activity of the patient's serum was contained within the IgM fractio...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80002-8
更新日期:1982-02-01 00:00:00
abstract::Alternative macrophage activation is largely defined by IL-4Rα stimulation but the contribution of Toll-like receptor (TLR) signaling to this phenotype is not currently known. We have investigated macrophage activation status under Th2 conditions in the absence of the core TLR adaptor molecule, MyD88. No impairment wa...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.07.006
更新日期:2013-04-01 00:00:00
abstract::Non-antigen-specific activation of human B lymphocytes for IgE production in vitro requires the presence of interleukin 4 and non-cognate physical interaction with T cells. The latter can be replaced by antibodies directed against the B cells' CD40 structure. Antigen-specific induction of immunoglobulin responses, inc...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80234-2
更新日期:1993-07-01 00:00:00
abstract::Fifty-nine patients with metastatic malignant melanoma were entered into a phase II trial of recombinant alpha-2 interferon given in a dosage of 10 million IU/M2 subcutaneously three times per week for one year. Forty-five of these were evaluable for response. Of five evaluable patients with ocular primaries, none res...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(86)80109-7
更新日期:1986-09-01 00:00:00
abstract:INTRODUCTION:The interaction between the immune system and malignant diseases is a proven key target for cancer therapy. We describe an innovative 3D cell culture system comprising both immune and cancer cells to evaluate their interaction and immune cell infiltration to provide an innovative in vitro screening of immu...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.12.010
更新日期:2016-05-01 00:00:00
abstract::Immunological mechanisms of hygiene hypothesis are expected to develop a novel strategy for allergy prevention. Although a large number of studies has investigated the relation between allergies and infection, little is known about the influence of the exposure to infections on antigen uptake by dendritic cells (DCs)....
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.10.018
更新日期:2018-01-01 00:00:00
abstract::The ability of RPMI 1640 enriched with FCS or AATSZ (L-alanine, BSA, human transferrin, zinc chloride, and sodium selenite) to support mitogen-induced activation (G0-G1 shift) and proliferation (G1-S shift) of thymocytes has been investigated. The two culture media were found to be equally supportive. In terms of viab...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80046-6
更新日期:1982-05-01 00:00:00
abstract:CONTEXT:The PDS gene (SLC26A4) is responsible for Pendred syndrome (PS). Genetic analysis of PDS using Tunisian samples showed evidence for linkage and association with autoimmune thyroid diseases (AITD) emergence. In addition, the PDS gene product, pendrin, was recently identified as a novel autoantigen in Graves' dis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2010.09.015
更新日期:2011-05-01 00:00:00
abstract::Hematopoietic cells express multiple receptors which bind the Fc domain of IgG. We utilized transfection of COS-1 cells, a cell line which lacks endogenous Fc receptors, to study the expression and function of Fc gamma RI, the high affinity Fc gamma receptor in the absence of other Fc gamma receptors. Fc gamma RI was ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80640-6
更新日期:1992-08-01 00:00:00
abstract::The lymphotoxin beta receptor (LTbetaR) mediates crucial signals in host defense against intracellular bacteria and viruses. Mice deficient in LTbetaR readily succumb to infections with Listeria monocytogenes, Mycobacterium tuberculosis and murine cytomegalovirus (mCMV). LTbetaR has been shown to be important for the ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2007.10.011
更新日期:2008-01-01 00:00:00
abstract::Dendritic cells (DCs) play a key role in the orchestration of the immune system by virtue of their capacity to control both immunity and tolerance induction. The functions of DCs depend on the subset as well as their location and activation state. A number of in vitro protocols, developed to recapitulate DC generation...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2008.07.017
更新日期:2008-01-01 00:00:00
abstract::Macrophage functions in the immune response depend on their ability to infiltrate tissues and organs. The penetration between and within the tissues requires degradation of extracellular matrix (ECM), a function performed by the specialized, endopeptidase- and actin filament- rich organelles located at the ventral sur...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.07.009
更新日期:2018-11-01 00:00:00
abstract::We studied local events in the popliteal lymph nodes of CD4-deficient mice following foot pad injection with an MMTV strain which carries the gene for a V beta 14-specific superantigen. Injection of the V beta 14-specific MMTV induced vigorous expansion of V beta 14+ CD4+ T cells and B cells in their lymph nodes of CD...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(96)80053-2
更新日期:1996-08-01 00:00:00