Abstract:
:When monocytes are stimulated with LPS (lipopolysaccharide) repeatedly then the initially high expression of the TNF (tumor necrosis factor) gene is only very low, i.e. the cells are tolerant to LPS. Tolerant cells still express the CD14 receptor and they can still be activated to mobilize NF-kappa B into nucleus. Analysis of the binding proteins employing the -605 motif of the human TNF promoter (GGGGCTGTCCC) revealed that in tolerant cells of the human monocytic cell line Mono Mac 6 there is a predominance of p50p50 of NF-kappa B. We now show that a mutant motif that exchanges the terminal 3' C for a G fails to bind the p50 homodimer that is upregulated in LPS toler ant human Mono Mac 6 cells. The same is true for nuclear extracts taken from the murine P388D1 macrophage cell line when tested with the -516 motif of the murine TNF promoter (GGGGGCTTTCCC). Here the wild type motif gives efficient binding of p50p50 that again is upregulated in tolerant cells whereas a mutant with a 3' G shows hardly any binding of p50p50. Conversely, the murine kappa light chain enhancer motif (GGGGACTTTCCG) does not efficiently bind the nuclear p50p50 from tolerant murine P388 macrophages. Binding is, however, readily detected when the 3' G is replaced by a C. These data show that the detection of upregulated p50 homodimers in LPS tolerant cells is dependent on subtle differences in the sequence of the DNA binding motif.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Frankenberger M,Ziegler-Heitbrock HWdoi
10.1016/s0171-2985(97)80029-0subject
Has Abstractpub_date
1997-12-01 00:00:00pages
81-90issue
1-3eissn
0171-2985issn
1878-3279pii
S0171-2985(97)80029-0journal_volume
198pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::Glypican-3 (GPC3) is an attractive target for chimeric antigen receptor (CAR)-T cell therapy, as it is overexpressed in most hepatocellular carcinoma (HCC) tissues but shows restricted expression in healthy adult tissues. Herein, we generated GPC3-specific CAR-T cells for HCC therapy by electroporation with plasmid DN...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.09.009
更新日期:2020-01-01 00:00:00
abstract::Autoantibodies against DNA topoisomerase I (anti-topo I) have been reported to be specific to systemic sclerosis (SSc), however, anti-topo I was detected in patients with silicone breast implants, SLE without features of SSc, and rheumatic diseases. We detected anti-topo I positive silicosis patients without any sympt...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00055
更新日期:2001-12-01 00:00:00
abstract::Activity of acid phosphatase was found to be higher in T than in non-T cells of human peripheral blood lymphocytes. The T cell mitogen PHA induced an increase in the magnitude of acid phosphatase activity, while the B cell mitogen LPS was not able to produce any effect on acid phosphatase activity. Acid phosphatase ac...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(85)80033-4
更新日期:1985-03-01 00:00:00
abstract::Individuals infected with Strongyloides stercoralis have been reported to produce different immunoglobulins isotypes, yet few studies have evaluated their use in strongyloidiasis diagnosis. The aim of this work was to evaluate the immunoreactivity of different classes and subclasses of anti-S. stercoralis circulating ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.151898
更新日期:2020-03-01 00:00:00
abstract::Splenic B cells of BALB/c mice were stimulated in vitro either with lipopolysaccharide (LPS), dextran sulphate (DxS), or with both LPS and DxS. The absolute frequency of B cells that differentiate into clones of immunoglobulin (Ig)-secreting cells upon activation with these mitogens was determined by means of limiting...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(88)80060-3
更新日期:1988-02-01 00:00:00
abstract::The tumor suppressor protein P53 is strongly involved in orchestrating cellular defenses in the diverse variety of human tissues. Anomalies to lung endothelium permeability are streaming severe consequences towards human health, often associated with fatal outcomes. Ongoing investigations suggest that P53 exerts a pro...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.04.008
更新日期:2019-07-01 00:00:00
abstract::The part played by MHC antigens on lymphocyte homing has been investigated by inducing modifications of their expression on lymphoid cells. A significant, though moderate, enhancement of H-2 antigen expression has been observed following in vitro treatment of lymphocytes with alpha-IFN. A decrease has been obtained af...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(84)80073-X
更新日期:1984-12-01 00:00:00
abstract::We examined the expression of seven V alpha or V beta T cell receptor (TCR) segments on human CD4+ and CD8+ T cells. Confirming previously published results, we found a preferential expression of four V segment gene products on CD4+ T cells. One of these markers (V beta 6.7) was constantly expressed on more CD4+ T cel...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80242-1
更新日期:1993-01-01 00:00:00
abstract::In order to evaluate the role of CD8+ T cells in cutaneous leishmaniasis, major histocompatibility complex (MHC) class II-deficient mice of C57BL/6 background lacking functional CD4+ T cells were infected with Leishmania major. In contrast to C57BL/6 wild-type mice which are resistant to infection with L. major, these...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(96)80043-X
更新日期:1996-07-01 00:00:00
abstract:BACKGROUND:Ficolins serve as soluble recognition molecules in the lectin pathway of complement. They are known to participate in the systemic host-response to infection but their role in local pulmonary defence is still incompletely understood. The purpose of this study was to clarify whether acute lung and systemic in...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.11.015
更新日期:2016-05-01 00:00:00
abstract:BACKGROUND:Male reproductive tract infection and inflammation are important aetiological factors of infertility. Experimental Autoimmune Orchitis (EAO) is a model of chronic inflammation useful to study mechanisms of inflammatory reactions leading to testicular impairment. EAO is characterised by interstitial cell infi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.04.007
更新日期:2012-08-01 00:00:00
abstract::Interleukin (IL)-27, a member of the IL-6/IL-12 heterodimeric cytokine family, induces pro-inflammatory responses including early T helper (Th)1 differentiation and generation of cytotoxic T lymphocytes, and also anti-inflammatory responses including the differentiation to IL-10-producing regulatory T cells, inhibitio...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.07.028
更新日期:2013-04-01 00:00:00
abstract::Regulatory properties of macrophages associated with alternative activation serve to limit the exaggerated inflammatory response during pneumonia caused by Pseudomonas aeruginosa infection. Arginase-1 is an important effector of these macrophages believed to play an essential role in decreasing injury and promoting re...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2020.152034
更新日期:2020-11-24 00:00:00
abstract::Telomere erosion and residual replicative capacity can be used as markers of the replicative history of somatic cells. We have investigated telomere length, in vitro replicative capacity and rate of telomere erosion in T and B lymphocyte populations from patients with primary antibody deficiency requiring immunoglobul...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00292
更新日期:2004-01-01 00:00:00
abstract::Trypanosoma cruzi is a real challenge to the host's immune system, because it requires strong humoral and cellular immune response to remove circulating trypomastigote forms, and to prevent the replication of amastigote forms in tissues, involving many regulator and effector components. This protozoan is responsible f...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.10.003
更新日期:2015-03-01 00:00:00
abstract::The aim of the work was to justify the use of trifluoromethyl-dinitrobenzene sulfonate (CF3-DNBS) modification rather than trinitrobenzene-sulfonate (TNBS) modification, so as to be able to take advantage of the presence of fluorine atoms in the analogue, which allow the analysis of the hapten-carrier bonds by 19F-NMR...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80012-6
更新日期:1983-02-01 00:00:00
abstract::Equine dendritic cells (eqDC) can be generated from peripheral blood monocytes by propagation in GM-CSF and IL-4. Despite similarities with the generation of human DC, we found significant improvements for eqDC generation and functional influences on eqDC maturation. The fractionation of peripheral blood mononuclear c...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2008.07.024
更新日期:2008-01-01 00:00:00
abstract::The polymorphonuclear neutrophil (PMN) regulates in vivo and in vitro immune responses. We report that the immunoenhancing properties of PMN culture supernatants from PMN recruited by the bacterium Actinomyces viscosus (AV) show its exclusive effects on the T cell lymphocyte population. A study of the effect of PMN su...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(83)80066-7
更新日期:1983-12-01 00:00:00
abstract::By fusion of C3H/HeJ splenic adherent mononuclear cells enriched for macrophages with HPRT-deficient C57L/J HH- hepatoma cells, we have generated six macrophage-hepatoma hybrid clones. The hybrid nature of isolated clones was demonstrated by karyotypic analysis. The hybrid clones were screened for macrophage propertie...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80017-7
更新日期:1987-08-01 00:00:00
abstract:BACKGROUND:An association between Tako-Tsubo cardiomyopathy (TTC) and underlying malignancies has been observed, suggesting that TTC might be the consequence of paraneoplastic phenomena. This study investigates the presence of autoantibodies against cardiomyocytes as well as adrenergic (β1, β2) and muscarinic (M2) rece...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2018.11.005
更新日期:2019-03-01 00:00:00
abstract::Peritoneal as well as peripheral blood, but not spleen macrophages inhibit natural killer (NK) cell activity directed against adherent growing target cells. Inhibition was observed with macrophages (Mø): spleen cell ratios as low as 0.01:1. It was independent from the activation status of Mø and was not MHC restricted...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(82)80063-6
更新日期:1982-12-01 00:00:00
abstract::Asthma is an airway inflammatory disorder. Vitamin (Vit) D is a potent immuno-modulator. It suppresses Interleukin (IL)-17 and induces IL-10. This study aims to investigate the role of IL-17A and IL-10 in predicting asthma control in case of Vit D supplementation. Seventy-nine patients enrolled in this study (42 patie...
journal_title:Immunobiology
pub_type: 杂志文章,随机对照试验
doi:10.1016/j.imbio.2019.09.005
更新日期:2019-11-01 00:00:00
abstract::Understanding the mechanisms governing the type of induced immune response after microbial invasion, could be of crucial importance for the rational design of a bacteria-based vaccine. Targeting a vaccine directly to dendritic cells (DCs), which are considered the most powerful antigen presenting cells, could be extre...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00094
更新日期:2001-12-01 00:00:00
abstract::Recently, it could be shown that Cyclosporin A (CsA) inhibited the activation of T and B lymphocytes by interfering with an early step of the activation, namely the stimulation of the plasma membrane-bound lysophosphatide acyltransferase. In this report, we compared three CsA-derivatives, Dihydro-Cyclosporin C (Dihydr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80025-6
更新日期:1987-09-01 00:00:00
abstract::The major target organ of systemic infection with the intracellular bacterium Listeria monocytogenes is the liver, to where inflammatory leukocytes are rapidly recruited. We determined by reverse transcriptase polymerase chain reaction the early chemokine response in the liver after systemic infection of mice with lis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(98)80066-1
更新日期:1998-07-01 00:00:00
abstract::Living organisms have strong defense mechanisms against invading microorganisms as survival strategies. One of the defense mechanisms is the complement system, composed of more than 30 serum and cell surface components. This system collaborates in recognition and elimination of pathogens as a part of both the innate a...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2006.01.003
更新日期:2006-01-01 00:00:00
abstract::During bacterial peritonitis of patients on continuous ambulatory peritoneal dialysis (CAPD) leukocytes, particularly polymorphonuclear neutrophilic granulocytes (PMNs), migrate into the peritoneal cavity. However, at the site of inflammation PMNs are not sufficiently able to protect the host against micro-organisms. ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80430-4
更新日期:1995-08-01 00:00:00
abstract::Ficolins are a group of proteins containing both a collagen-like domain and a fibrinogen-like domain and are found in varying tissues. Ficolins present in sera have a lectin activity toward N-acetylglucosamine through their fibrinogen-like domains. The domain organizations between ficolins and mannose-binding lectin (...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1078/0171-2985-00149
更新日期:2002-09-01 00:00:00
abstract::Chemokines are important mediators of leukocyte recruitment and activation that play critical roles in the pathology of inflammatory diseases such as atherosclerosis, rheumatoid arthritis and asthma. The vaccinia virus (strain Lister) expresses a 35 kDa soluble protein ('35K') that binds and inactivates a wide range o...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00228
更新日期:2003-01-01 00:00:00
abstract::Functional polymorphisms in RNA recognizing toll like receptors (TLR) 3, 7, 8 and toll-interleukin-1 receptor domain containing adapter protein adapter (TIRAP) coding genes were investigated in 120 dengue cases [87 dengue fever (DF) cases and 33 dengue hemorrhagic fever (DHF) cases] and 109 healthy controls (HC) to id...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.09.020
更新日期:2015-01-01 00:00:00