Abstract:
:The aim of the work was to justify the use of trifluoromethyl-dinitrobenzene sulfonate (CF3-DNBS) modification rather than trinitrobenzene-sulfonate (TNBS) modification, so as to be able to take advantage of the presence of fluorine atoms in the analogue, which allow the analysis of the hapten-carrier bonds by 19F-NMR nuclear magnetic resonance. Cell-surface antigenic modifications brought about by exposure to TNBS or CF3-DNBS were found to be immunologically cross-reactive, both in cell-mediated lymphocytotoxicity and in indirect immunofluorescence. The extent of haptenic derivatizations was found to be of the same order of magnitude, as appraised both by quantitative-absorption studies or by using radioactive hapten, provided that the less chemically reactive CF3-DNBS was used at the concentration of 10 mM and TNBS at the concentration of 1 mM. However, only TNBS-modified cells were sensitive to destruction by antibody-plus-complement-mediated cytotoxicity.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Bischoff P,Maugras M,Llorens MC,Oth Ddoi
10.1016/S0171-2985(83)80012-6subject
Has Abstractpub_date
1983-02-01 00:00:00pages
1-12issue
1eissn
0171-2985issn
1878-3279pii
S0171-2985(83)80012-6journal_volume
164pub_type
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