Abstract:
:This study investigated the actin scavenger function of the vitamin D binding protein (DBP) in vivo using DBP null (-/-) mice. Intravenous injection of G-actin into wild-type (DBP+/+) and DBP-/- mice showed that contrary to expectations, DBP+/+ mice developed more severe acute lung inflammation. Inflammation was restricted to the lung and pathological changes were clearly evident at 1.5 and 4h post-injection but were largely resolved by 24h. Histology of DBP+/+ lungs revealed noticeably more vascular leakage, hemorrhage and thickening of the alveolar wall. Flow cytometry analysis of whole lung homogenates showed significantly increased neutrophil infiltration into DBP+/+ mouse lungs at 1.5 and 4h. Increased amounts of protein and leukocytes were also noted in bronchoalveolar lavage fluid from DBP+/+ mice 4h after actin injection. In vitro, purified DBP-actin complexes did not activate complement or neutrophils but induced injury and death of cultured human lung microvascular endothelial cells (HLMVEC) and human umbilical vein endothelial cells (HUVEC). Cells treated with DBP-actin showed a significant reduction in viability at 4h, this effect was reversible if cells were cultured in fresh media for another 24h. However, a 24-h treatment with DBP-actin complexes showed a significant increase in cell death (95% for HLMVEC, 45% for HUVEC). The mechanism of endothelial cell death was via both caspase-3 dependent (HUVEC) and independent (HLMVEC) pathways. These results demonstrate that elevated levels and/or prolonged exposure to DBP-actin complexes may induce endothelial cell injury and death, particularly in the lung microvasculature.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Ge L,Trujillo G,Miller EJ,Kew RRdoi
10.1016/j.imbio.2013.10.001subject
Has Abstractpub_date
2014-03-01 00:00:00pages
198-207issue
3eissn
0171-2985issn
1878-3279pii
S0171-2985(13)00176-9journal_volume
219pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract::The phagocyte pattern recognition receptor Toll-like receptor 2 (TLR2) and the multi-receptor adaptor MyD88 contribute to the reduction of bacterial load in infections with intra- and extra-cellular Gram-positive bacteria. Their mechanism of antibacterial action is mostly unresolved but evident in vivo by an increased...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2005.05.017
更新日期:2005-01-01 00:00:00
abstract::Tumor necrosis factor superfamily (TNFSF) represents a group of multifunctional inflammatory cytokines that have been shown to participate in a variety of pathological and immunological process. However, the functions of these proteins in oyster are still poorly understood. In the present study, an oyster TNF homolog ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.02.002
更新日期:2017-05-01 00:00:00
abstract:UNLABELLED:The lymphoid tissue of the appendix is considered as part of the gut-associated lymphoid tissue (GALT). In order to understand better the immunological significance of the appendix we analyzed the cellular composition of normal and inflamed human appendix tissue by flow cytometer with special attention to ex...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(00)80098-4
更新日期:2000-01-01 00:00:00
abstract::Memory T cells producing interferon (IFN)γ and expressing very late antigen-1 (VLA-1) integrin collagen receptors are found in carotid atherosclerotic plaques, suggesting their involvement in coronary artery disease (CAD) as well. To determine the role of VLA-1+ T cells in CAD percent of CD3+ T cells binding monoclona...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.11.005
更新日期:2014-04-01 00:00:00
abstract::The defective antigen presenting ability of antigen presenting cells (APCs) modulates host cytokines and co-stimulatory signals that may lead to severity of leprosy. In the present study, we sought to evaluate the phenotypic features of APCs along with whether DC SIGN (DC-specific intercellular adhesion molecule-grabb...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2013.05.004
更新日期:2013-12-01 00:00:00
abstract::When sheep red blood cells (SRBC) were injected intraperitoneally (i.p.), intravenously (i.v.), or into the Peyer's patches, definite plaque-forming cell (PFC) and rosette-forming cell (RFC) responses were induced in the spleen but not in the mesenteric lymph nodes (MLN). When SRBC were injected directly into the sple...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(82)80047-8
更新日期:1982-05-01 00:00:00
abstract::Bone marrow-derived macrophages (BMM) comprise a population of quiescent cells which can be activated by defined signals. Here, we directly compare the release of chemokines and monokines by BMM raised either in serum-supplemented or in serum-free medium in response to Listeria monocytogenes EGD or Mycobacterium bovis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(99)80037-0
更新日期:1999-02-01 00:00:00
abstract::Recently, it could be shown that Cyclosporin A (CsA) inhibited the activation of T and B lymphocytes by interfering with an early step of the activation, namely the stimulation of the plasma membrane-bound lysophosphatide acyltransferase. In this report, we compared three CsA-derivatives, Dihydro-Cyclosporin C (Dihydr...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80025-6
更新日期:1987-09-01 00:00:00
abstract::Autoimmune lymphoproliferative syndrome (ALPS) is a primary immunodeficiency caused by impaired Fas/FasL-mediated apoptosis of lymphocytes and is characterized by chronic nonmalignant or benign lymphoproliferation, autoimmune manifestations and expansion of double negative (DN) T-cells (TCRαβ+CD4-CD8-). Most cases of ...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2015.08.004
更新日期:2016-01-01 00:00:00
abstract::Chemokines are important mediators of leukocyte recruitment and activation that play critical roles in the pathology of inflammatory diseases such as atherosclerosis, rheumatoid arthritis and asthma. The vaccinia virus (strain Lister) expresses a 35 kDa soluble protein ('35K') that binds and inactivates a wide range o...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1078/0171-2985-00228
更新日期:2003-01-01 00:00:00
abstract::Immunosuppressive effects of corticosteroids are generally known, but the mechanisms influencing the immune system are poorly understood. However, a correlation between cortisol levels and cytokine production has been reported by several investigators. In the present study we determined cortisol concentrations in huma...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(99)80072-2
更新日期:1999-06-01 00:00:00
abstract::Ficolins and mannan-binding lectin recognize pathogen-associated molecular patterns and initiate the lectin pathway of complement activation via the associated serine proteases. In contrast to human ficolins and mouse ficolin-A, mouse ficolin-B has been considered incapable of complement activation. Dose-dependent bin...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.01.013
更新日期:2012-10-01 00:00:00
abstract::Human Lymphocyte Associated Antigen-1 (LFA-1, CD11a/CD18, alpha L/beta 2) and p150,95 (CD11c/CD18, alpha X/beta 2) are cell surface alpha/beta heterodimers that, together with Mac-1 (CD11b/CD18, alpha M/beta 2) comprise the leukocyte-restricted beta 2 subfamily of integrins. LFA-1 is the only integrin expressed on all...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80560-7
更新日期:1995-07-01 00:00:00
abstract::Equine dendritic cells (eqDC) can be generated from peripheral blood monocytes by propagation in GM-CSF and IL-4. Despite similarities with the generation of human DC, we found significant improvements for eqDC generation and functional influences on eqDC maturation. The fractionation of peripheral blood mononuclear c...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2008.07.024
更新日期:2008-01-01 00:00:00
abstract::Periodontitis is a chronic oral pathology caused by impaired immune response against oral bacteria resulting in tissue inflammation and damage. Among the members of innate immune response, the first line of defence against pathogens, inflammasomes are macro-molecular protein complexes that can be activated by differen...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2017.01.001
更新日期:2017-05-01 00:00:00
abstract::Cryptococcus neoformans is a pathogenic fungus causing life threatening infections in humans. The present in vitro study aimed to investigate the opsonizing properties of a well characterized serum ficolin (rat ficolin-A), a member of carbohydrate-recognition molecules of the innate immune system, in the defence again...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2012.06.006
更新日期:2013-04-01 00:00:00
abstract::Three different anti-CD26 monoclonal antibodies (mAbs) are described, which specifically inhibited proliferation of human T lymphocytes after stimulation with PHA, tetanus toxoid or soluble anti-CD3 mAb. Anti-CD26 mAbs induced in T cells a dose-dependent shift of the maximum of DNA synthesis, which was due to a transi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80485-7
更新日期:1993-06-01 00:00:00
abstract::The course of legionella pneumonia in guinea pigs after infection with various quantities of virulent L. pneumophila serogroup 1 organisms by aerosol exposure was investigated. The clinical course, histopathological characteristics, manifestations in the lungs and clearance of the legionella organisms from the lungs a...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(87)80104-3
更新日期:1987-12-01 00:00:00
abstract::One of the most important effects of aging is the decline of immune effectiveness. A study is described here on the primary (IgM) antibody response to phosphorylcholine (PC), an antigen expressed on the surface of the Streptococcus pneumoniae R36a, in aged (18-22 mo.) C57BL/6 mice. Preliminary data showed a significan...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(11)80288-3
更新日期:1994-02-01 00:00:00
abstract::It is well known that adherence of monocytes (MO) to extracellular matrix substrates or tissue culture plastic activates these cells and induces the expression of a multitude of genes. Especially, it was described, that MO are primed by cell adhesion to produce higher amounts of some cytokines, e.g. interleukin (IL)-8...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(97)80069-1
更新日期:1996-01-01 00:00:00
abstract::The mechanisms of tolerance induction occurring in the course of allergen-specific immunotherapy have not been elucidated in full detail. Our study aimed to characterize high zone tolerance in mouse models of type I allergy and of allergic airway inflammation induced by subcutaneous sensitization of mice with high dos...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2014.10.022
更新日期:2015-04-01 00:00:00
abstract::Human sera enhanced spontaneous cell-mediated cytotoxicity (SCMC), while anti-IgG (Fab') 2 treatment decreased this cytotoxic activity of human lymphocytes for an in vitro growing cell line (K--562). Trypsin treatment of the effector cells considerably decreased the cytotoxic potential. However, a significant cytotoxi...
journal_title:Immunobiology
pub_type: 杂志文章
doi:
更新日期:1979-09-01 00:00:00
abstract::A cyclophosphamide (CP)-induced tolerance system in mice that primarily consists of donor cell injection followed by CP-treatment was found useful for inducing a long-lasting allo- or xeno-tolerance to various solid organs. In the cells-followed-by-CP system, the sequential mechanisms of tolerance were clarified using...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/S0171-2985(96)80033-7
更新日期:1996-07-01 00:00:00
abstract::Soluble MHC antigens are detected in body fluids but their role and origin are still unclear. This study examined whether serum IA antigens, isolated from BALB/c mice (sIA(d)), could modulate the immune response. Specific purification procedures isolated intact IA molecules, which were thereafter applied to functional...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2009.04.005
更新日期:2010-03-01 00:00:00
abstract::To date, numerable reviews are found in the literature prominent to the effect of caffeine on the immune system, with the latest review published in 2006. Database screening reveals around three thousand articles that have been published during the last decade. Interestingly, less than hundred articles involved humans...
journal_title:Immunobiology
pub_type: 杂志文章,评审
doi:10.1016/j.imbio.2018.08.011
更新日期:2018-12-01 00:00:00
abstract::Details of the "Trojan Horse" mechanism by which Zika virus (ZIKV) crosses the blood-brain barrier (BBB) remain unclear. However, the migration of ZIKV-infected monocytes to the brain is thought to be dependent on both pattern-recognition and chemokine receptors. In this study, we investigated whether the migration of...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2019.08.007
更新日期:2019-11-01 00:00:00
abstract::The major target organ of systemic infection with the intracellular bacterium Listeria monocytogenes is the liver, to where inflammatory leukocytes are rapidly recruited. We determined by reverse transcriptase polymerase chain reaction the early chemokine response in the liver after systemic infection of mice with lis...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/S0171-2985(98)80066-1
更新日期:1998-07-01 00:00:00
abstract::Antibody-mediated elimination of foreign antigens contributes to immune protection from viral infection. We have generated phycoerythrin-conjugated HIV-1 Gag/p24 and influenza virus nucleoprotein tetramers for flow cytometric analysis of blood cell populations involved in antibody-mediated immunity. We show that in th...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/j.imbio.2009.02.004
更新日期:2010-03-01 00:00:00
abstract::Based on our findings that HIV-1 gp41 independently of CD4 can bind to the human helper T lymphoid cell line H9, B cell line Raji and monocyte cell line U937, we characterized putative binding of HIV-1 gp41 to human peripheral blood lymphocytes (PBLs) and monocytes. Using flow cytometry (FACS), we demonstrated that th...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(11)80216-0
更新日期:1993-08-01 00:00:00
abstract::In a rat model, we compared the effects of various soluble products released by T cells, such as the unspecifically acting gamma-interferon or the newly detected alloantigen-induced factors that specifically act on nonlymphoid, hemopoietic bone marrow cells. We found two types of reactivity patterns with regard to the...
journal_title:Immunobiology
pub_type: 杂志文章
doi:10.1016/s0171-2985(87)80096-7
更新日期:1987-12-01 00:00:00