Abstract：
UNLABELLED:The lymphoid tissue of the appendix is considered as part of the gut-associated lymphoid tissue (GALT). In order to understand better the immunological significance of the appendix we analyzed the cellular composition of normal and inflamed human appendix tissue by flow cytometer with special attention to expression of the CD19 and CD5 markers on B cells. Cellular analysis was also performed on peripheral and appendical vein blood samples as well as on omentum and peritoneal fluid samples. The study population included seventeen patients aged 2-15 yr. (mean age - 11.5 yr.) undergoing appendectomy. Ten children were diagnosed with acute appendicitis while 7 had a normal appendix. RESULTS:Compared to the peripheral blood, the appendix contained a significantly higher percentage of CD19 cells (47.6% of total lymphocytes versus 15%, p<0.0001), and B1 cells (4.98% of total lymphocytes versus 2.42%, p=0.001). In addition, the intensity of CD19-staining was markedly decreased in the appendix (mean - 395.7), and also in the omentum (mean - 398.2) as compared to peripheral lymphocytes (mean - 497.7, p<0.0001 for both comparison). Comparison between the inflamed and the non-inflamed appendices revealed that the inflamed appendix contained a significantly higher proportion of B1 cells (5.64% of total lymphocytes versus 3.53%, p=0.032), and also a higher B1/b cell ratio (0.13 vs. 0.07, p=0.01). CONCLUSIONS:These results indicate that the appendix tissue contains higher number of B1 (and B) cells compared to the peripheral blood and that these cells play a role in the primary immune response to acute infection/inflammation in the appendix. Appendiceal B cell population is unique in term of CD19 intensity expression on their surface.
journal_name
Immunobiologyjournal_title
Immunobiologyauthors
Somekh E,Serour F,Gorenstein A,Vohl M,Lehman Ddoi
10.1016/S0171-2985(00)80098-4subject
Has Abstractpub_date
2000-01-01 00:00:00pages
461-9issue
3-4eissn
0171-2985issn
1878-3279pii
S0171-2985(00)80098-4journal_volume
201pub_type
杂志文章相关文献
IMMUNOBIOLOGY文献大全abstract：:Ribosomal 30S and 50S subunits as well as total proteins from the large subunit of E. coli ribosomes were found to be potent mitogens for both human adult and cord blood lymphocytes. On the other hand, the RNA fraction of the 50S subunit had only a weak stimulatory capacity suggesting that the protein moiety contains ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(80)80079-9
更新日期：1980-01-01 00:00:00
abstract：:A heterogeneous set of cells that are commonly grouped as "myeloid cells", interacts in a complex landscape of physiological and pathological situations. In this review we attempt to trace a profile of the "myeloid connection" through different normal and pathological states, by analyzing common metabolic pathways of ...
journal_title：Immunobiology
pub_type： 杂志文章,评审
doi：10.1016/j.imbio.2007.09.008
更新日期：2007-01-01 00:00:00
abstract：:High Mphi:T cell ratios suppress the immune response to the retroviral superantigen Mls by IFNgamma-triggered production of the arg- and trp-consuming enzymes iNOS and IDO. Attempts to reverse suppression by treatment with pro-inflammatory cytokines revealed that IL-6 improved the T cell response to Mls and the pro-he...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2009.01.015
更新日期：2010-01-01 00:00:00
abstract：:It is established that (1) subclinical Bordetella pertussis colonization of the nasopharynx persists in highly vaccinated populations, and (2) B. pertussis toxin is a potent adjuvant that, when co-administered with neural antigens, induces neuropathology in experimental autoimmune encephalomyelitis, the principle anim...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2015.12.008
更新日期：2016-04-01 00:00:00
abstract：:Airway epithelial cells induce a tolerogenic microenvironment by modulating immune cells in the lung. We recently showed that the supernatant of airway epithelial cells induces two marker genes of alternative activation, Ym1 and Ms4a8a, in respiratory myeloid cells. This induction was partially mediated by glucocortic...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2017.02.003
更新日期：2017-05-01 00:00:00
abstract：:In contrast to murine spleen M phi, resident peritoneal M phi from health mice express very little Forssman glycolipid antigen (Fo). The following experiments suggest that Fo expression by peritoneal M phi may be associated with inflammation. Balb/c and CBA/J mice were given inflammatory stimuli by i.p. injection of l...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/s0171-2985(11)80302-5
更新日期：1990-06-01 00:00:00
abstract：:This study demonstrates immune complexes in thyroid glands of Obese strain (OS) chickens, that consist of thyroglobulin (Tg) and antibodies to Tg. In IIF tests it was shown that these complexes fix complement with an age-dependent increase from 12% in 19-day-old embryos up to 100% in 6-week-old animals. This finding a...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(83)80035-7
更新日期：1983-05-01 00:00:00
abstract：:Bone marrow-derived macrophages (BMM) comprise a population of quiescent cells which can be activated by defined signals. Here, we directly compare the release of chemokines and monokines by BMM raised either in serum-supplemented or in serum-free medium in response to Listeria monocytogenes EGD or Mycobacterium bovis...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(99)80037-0
更新日期：1999-02-01 00:00:00
abstract：:To date, numerable reviews are found in the literature prominent to the effect of caffeine on the immune system, with the latest review published in 2006. Database screening reveals around three thousand articles that have been published during the last decade. Interestingly, less than hundred articles involved humans...
journal_title：Immunobiology
pub_type： 杂志文章,评审
doi：10.1016/j.imbio.2018.08.011
更新日期：2018-12-01 00:00:00
abstract：:The leukocyte integrins CD11a/CD18 (LFA-1, alphaLbeta2) and CD49d (VLA-4, alpha4beta1, alpha4beta7) mediate leukocyte transendothelial migration during immune and inflammatory responses and provides co-stimulatory signals for the activation of T lymphocytes. Our previous studies demonstrate that the CD11a gene promote...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2005.05.008
更新日期：2005-01-01 00:00:00
abstract：:Signal transduction by the Toll-like receptors (TLRs) is a key component of innate immunity against many pathogens and also underlies a large burden of human diseases. Therefore, the mechanisms and regulation of signaling from the TLRs are of considerable interest. Here we seek to determine the molecular mechanism by ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2019.12.004
更新日期：2020-03-01 00:00:00
abstract：:We investigated the effect of Lewis Lung carcinoma cells on the production of C3 by murine macrophages and examined the capacity of secreted C3 to opsonize Lewis Lung carcinoma cells. C3 released in culture from macrophages obtained from tumor-bearing C57Bl/6 mice as well as from normal macrophages exposed to Lewis Lu...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(88)80043-3
更新日期：1988-07-01 00:00:00
abstract：:OK-432, a streptococcal preparation, has been shown as an effective activator to induce human monocyte-derived dendritic cells maturation. During this process, the activation of Toll-like receptor 4 plays an important role. However, the signaling pathway involved in has not been fully understood. In the present study,...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2008.10.008
更新日期：2009-01-01 00:00:00
abstract：:The polymorphonuclear neutrophil (PMN) regulates in vivo and in vitro immune responses. We report that the immunoenhancing properties of PMN culture supernatants from PMN recruited by the bacterium Actinomyces viscosus (AV) show its exclusive effects on the T cell lymphocyte population. A study of the effect of PMN su...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(83)80066-7
更新日期：1983-12-01 00:00:00
abstract：:The goal of our work was to titer the IgG, IgM and IgA in Pentaglobin® (a preparation enriched in IgM), targeting specific surface antigens of Gram-positive and Gram-negative bacteria as well as a C. albicans strain. Lipopolysaccharides from Gram-negative bacteria, peptidoglycan and lipoteichoic acid from the other mi...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2019.12.006
更新日期：2020-03-01 00:00:00
abstract：:α-Synuclein plays a crucial role in Parkinson's disease and dementias defined as synucleinopathies. α-Synuclein is expressed in hematopoietic and immune cells, but its functions in hematopoiesis and immune responses are unknown. We utilized α-synuclein(-/-) (KO) mice to investigate its role in hematopoiesis and B cell...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2014.07.014
更新日期：2014-11-01 00:00:00
abstract：:A "lectin-like" receptor for bacterial cell wall sugars, originally described in the membrane of mouse peritoneal macrophages, which facilitates binding of various bacteria to the macrophage surface, has now been shown to be present on most phagocytes from a variety of species. The role of such a receptor in the infla...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(82)80090-9
更新日期：1982-04-01 00:00:00
abstract：:Lymphocytes from spleen, thymus and lymph nodes from individual young adult (3--4 months) and aged (26--30 months) NMRI mice were stimulated with the mitogens Con A, PHA and LPS. 24 hours later, the number of cell with increased RNA-content (G1 cells) was determined by cytofluorometry. In parallel the 3H-thymidine inc...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(81)80014-9
更新日期：1981-06-01 00:00:00
abstract：:The aim of this study was to evaluate some immunological patterns involved in natural and acquired resistance against MHV3 using the original model of genetically modified lines of mice selected for high (HIII) and low (LIII) antibody responsiveness. As previously shown, a lower pre-existing anti-MHV antibody level wa...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1078/0171-2985-00239
更新日期：2003-01-01 00:00:00
abstract：:Th2 cells play a central role in type I allergies. However, the source of interleukin-4 which may lead to a Th1/Th2 imbalance is unknown. Valpha24+CD161+ Natural killer T (NKT) cells secrete high amounts of interleukin-4 and/or interferon-gamma and are assumed to participate in the initiation of Th1/Th2 immune respons...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1078/0171-2985-00284
更新日期：2003-01-01 00:00:00
abstract：:Interleukin-7 (IL-7) supports the proliferation of mature T lymphocytes, however, the cellular source of IL-7 for T lymphocyte activation has not been well established. We therefore investigated whether human peripheral blood dendritic cells (DC) produce IL-7 as a contribution towards T lymphocyte activation. Human CM...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(98)80075-2
更新日期：1998-03-01 00:00:00
abstract：:Numerous studies have shown that over-activation of microglia could cause neuroinflammation and release pro-inflammatory mediators, which could result in neurodegenerative diseases, like Parkinson's disease, Alzheimer's disease etc. Beta-naphthoflavone (BNF) has anti-oxidant and anti-inflammatory effects in borderline...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2020.151965
更新日期：2020-07-01 00:00:00
abstract：:Non-antigen-specific activation of human B lymphocytes for IgE production in vitro requires the presence of interleukin 4 and non-cognate physical interaction with T cells. The latter can be replaced by antibodies directed against the B cells' CD40 structure. Antigen-specific induction of immunoglobulin responses, inc...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(11)80234-2
更新日期：1993-07-01 00:00:00
abstract：:We investigated the mRNA expression of inducible nitric oxide synthase (iNOS) and the production of nitric oxide (NO) in human T cell lymphotropic virus type I (HTLV-I) p40tax-transfected U937 cells, a human monoblast cell line. Transfection of HTLV-I p40tax U937 cells induced up-regulation of iNOS mRNA expression and...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/s0171-2985(97)80083-6
更新日期：1997-11-01 00:00:00
abstract：:Cytophotometric measurements of DNA, dry mass (total proteins) and surface localized IgM and Ia were performed on individual cells of a Burkitt lymphoma derived cell line after X-irradiation and after polyploidization. Blockage of cell division was achieved with 250 r, and an arrest in G2 was found 48 hr after irradia...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(80)80099-4
更新日期：1980-07-01 00:00:00
abstract：:T-lymphocytes, including Th17-cells and T-cells expressing acetylcholine (ACh), are key components of systemic inflammation in chronic obstructive pulmonary disease (COPD). We investigated whether ACh promotes Th17 cells in COPD. ACh, IL-17A, IL-22, RORγt, FOXP3 expression and AChIL-17A, AChIL-22, AChRORγt coexpressio...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2014.01.004
更新日期：2014-05-01 00:00:00
abstract：:The suppressive activity of two bovine serum albumin-specific class II-restricted T suppressor cell clones (BVI/5 and 83/2-D11) was compared to that of a feeder cell-independent, IL 2-dependent subline (HF1.IL-2) of an originally antigen-dependent class II-restricted Ts cell clone (HF1). No soluble suppressor factors ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/S0171-2985(87)80008-6
更新日期：1987-05-01 00:00:00
abstract：:The heavy-chain variable regions (VH) from 14F7 MAb, an IgG1 antibody specific for GM3(NeuGc) ganglioside, and its anti-idiotype, the 4G9 MAb, were cloned and sequenced. Comparison with previously reported sequences showed that VH 14F7 belongs to the J558(VHI) gene family and that it is highly mutated. VH 4G9 belongs ...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2006.08.005
更新日期：2007-01-01 00:00:00
abstract：:The defective antigen presenting ability of antigen presenting cells (APCs) modulates host cytokines and co-stimulatory signals that may lead to severity of leprosy. In the present study, we sought to evaluate the phenotypic features of APCs along with whether DC SIGN (DC-specific intercellular adhesion molecule-grabb...
journal_title：Immunobiology
pub_type： 杂志文章
doi：10.1016/j.imbio.2013.05.004
更新日期：2013-12-01 00:00:00
abstract：:Respiratory viral infections have been implicated in the origin of, protection from and exacerbation of allergy-related symptoms in a variety of ways. Viral infections are closely linked to infantile wheezing. Severe bronchiolitis in early infancy may predispose to chronic childhood asthma as well as allergic sensitiz...
journal_title：Immunobiology
pub_type： 杂志文章,评审
doi：10.1016/j.imbio.2007.03.008
更新日期：2007-01-01 00:00:00
