Abstract：
:Sodium alginate is an effective biomaterial for tissue engineering applications. Non-purified alginate is contaminated with protein, lipopolysaccharide, DNA, and RNA, which could elicit adverse immunological reactions. We developed a purification protocol to generate biocompatible alginate based on (a) activated charcoal treatment, (b) use of hydrophobic membrane filtration (we used hydrophobic polyvinylidene difluoride membranes to remove organic contaminants), (c) dialysis, and finally (d) ethanol precipitation. Using this approach, we could omit pre-treatment with chloroform and significantly reduce the quantities of reagents used. Purification resulted in reduction of residual protein by 70% down to 0.315 mg/g, DNA by 62% down to 1.28 µg/g, and RNA by 61% down to less than 10 µg/g, respectively. Lipopolysaccharide levels were reduced by >90% to less than 125 EU/g. Purified alginate did not induce splenocyte proliferation in vitro. Three-dimensional scaffolds generated from purified alginate did not elicit a significant foreign body reaction, fibrotic overgrowth, or macrophage infiltration 4 weeks after implantation. This study describes a simplified and economical alginate purification method that results in alginate purity, which meets clinically useful criteria.
journal_name
J Biomater Appljournal_title
Journal of biomaterials applicationsauthors
Sondermeijer HP,Witkowski P,Woodland D,Seki T,Aangenendt FJ,van der Laarse A,Itescu S,Hardy MAdoi
10.1177/0885328216645952subject
Has Abstractpub_date
2016-10-01 00:00:00pages
510-520issue
4eissn
0885-3282issn
1530-8022pii
0885328216645952journal_volume
31pub_type
杂志文章abstract：:A biodegradable polylactic acid composite containing tricalcium phosphate microsphere was fabricated. The composite exhibited enhanced biocompatibility and a well-interconnected porous structure that enabled tissue ingrowth after degradation. The tricalcium phosphate microspheres had an average size of 106 ± 43 μm and...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328218763660
更新日期：2018-05-01 00:00:00
abstract：:Hemocompatibility is the goal for any biomaterial contained in extracorporeal life supporting medical devices. The hallmarks for hemocompatibility include nonthrombogenicity, platelet preservation, and maintained platelet function. Both in vitro and in vivo assays testing for compatibility of the blood/biomaterial int...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章,评审
doi：10.1177/0885328214538866
更新日期：2014-10-01 00:00:00
abstract：:Injectable thermo-sensitive hydrogels have a potential application in bone tissue engineering for their sensitivities and minimal invasive properties. Human dental pulp stem cells have been considered a promising tool for tissue reconstruction. The objective of this study was to investigate the proliferation and osteo...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328216661566
更新日期：2016-09-01 00:00:00
abstract：:Gold nanomaterials possess unique physical and chemical properties, which attracted much attention in recent years. As a new type of gold nanomaterials, gold nanostars (GNSTs) have been prepared and characterized in this study. GNSTs under near-infrared (NIR) light irradiation can exert not only cancer photothermal th...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215594481
更新日期：2015-11-01 00:00:00
abstract：:Hydrogels were prepared from 2-hydroxyethyl methacrylate and ethylene glycol dimethacrylate as cross-linking agent. Two different polymerization techniques were followed, namely bulk polymerization initiated by dibenzoyl peroxide and solution polymerization, in water, using a redox initiator system. Differences in the...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532829801200402
更新日期：1998-04-01 00:00:00
abstract：:The aim of this work was to study the cytotoxicity of different fractions of gold nanoparticles prepared by ultrasonic spray pyrolysis from gold scrap. The target cells were rat thymocytes, as a type of nonproliferating cells, and L929 mouse fibroblasts, as a type of continuous proliferating cells. Fractions 1 and 2, ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328210377536
更新日期：2012-01-01 00:00:00
abstract：:Composite hydrogel systems consisting of oxidized alginate, gelatin, and biphasic calcium phosphate were fabricated by the Schiff-base reaction and the effect of oxidation of alginate on the microstructure, material properties, and biocompatibility were evaluated. Alginate was modified by oxidizing the attached -OH gr...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211404265
更新日期：2012-09-01 00:00:00
abstract：:Aluminum-free glass ionomer cements (GICs) are under development for orthopedic applications, but are limited by their insufficient handling properties. Here, the addition of calcium polyphosphate (CPP) was investigated as an additive to an experimental zinc-silicate glass ionomer cement. A 50% maximum increase in wor...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215568985
更新日期：2015-07-01 00:00:00
abstract：:The aim of this study was to compare the osteogenic effects of periodontal ligament stem cells (PDLSCs) versus bone marrow mesenchymal stem cells (BMMSCs) in combination with Bio-Oss scaffolds on subcutaneous and critical-size defects in the immunodeficient rat calvarium. PDLSCs and BMMSCs were obtained from the same ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328214521846
更新日期：2014-08-01 00:00:00
abstract：:Two series of glasses of general formula (2-p) SiO2.1.1Na2O.CaO.pP2O5.xZnO (p=0.10, 0.20; x=0.0, 0.16, 0.35, and 0.78) have been analyzed for physico-chemical surface features before and after contact with simulated body fluid, morphological characteristics, and osteoblast-like cells behavior when cultured on them. Th...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328207079731
更新日期：2008-05-01 00:00:00
abstract：:This investigation describes the synthesis and in vitro evaluation of cationic hydrogel sub-microparticles based on polydimethylaminoethylmethacrylate for oral insulin delivery. Polymerization of dimethylaminoethylmethacrylate was carried out in aqueous medium with potassium persulfate as the initiator. Quaternization...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328212437392
更新日期：2013-07-01 00:00:00
abstract：PURPOSE:A functionalized graphene oxide-based thermosensitive hydrogel loaded with docetaxel for intratumoral delivery was designed to enhance therapeutic efficacy and alleviate system toxicity. METHODS:First, graphene oxide was functionalized with chitosan to acquire high stability in physiological solutions. And the...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215619583
更新日期：2016-03-01 00:00:00
abstract：:Hydroxyapatite (Ca10(PO4)6(OH)2) is widely investigated as an implantable material for hard tissue restoration due to its osteoconductive properties. However, hydroxyapatite in bulk form is limited as its mechanical properties are insufficient for load-bearing orthopedic applications. Attempts have been made to improv...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328215592866
更新日期：2015-11-01 00:00:00
abstract：:The carcinogenic potential of asbestos has led to the development of non-asbestos materials for use as casting ring liners. Cellulose paper are replacing asbestos paper. The brass die and crown were made to produce full crowns. The standardized wax pattern was replicated twenty-nine times. Two grooves were marked on t...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532820001400406
更新日期：2000-04-01 00:00:00
abstract：:Glass Polyalkenoate Cements (GPCs) based on strontium calcium zinc silicate (Sr-Ca-Zn-SiO2) glasses and high molecular weight poly(acrylic acid) (PAA) have been shown to exhibit suitable mechanical properties for orthopaedic arthroplasty applications, however for vertebroplasty and other medical luting applications th...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328208099085
更新日期：2010-02-01 00:00:00
abstract：:A multifunctional targeted nanoplatform combining photothermal therapy and chemotherapy has emerged as a promising strategy for comprehensive therapies of breast cancer. In this study, we constructed human epidermal growth factor receptor 2 (Her2)-targeted gold nanoshelled poly(lactic-co-glycolic acid) hybrid nanocaps...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328220929616
更新日期：2020-09-01 00:00:00
abstract：:Poor blood circulation makes it difficult for antitubercular drugs to achieve effective bactericidal concentration at tuberculose focus. The residual Mycobacterium tuberculosis around surgical wound would multiply, resulting in nonunion or sinus formation. Carbon nanotubes have strong tissue penetration and can cross ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328218814988
更新日期：2019-02-01 00:00:00
abstract：:In this study, a novel bilayer scaffold composed of electrospun polycaprolactone and poly(lacto-co-glycolic acid) (PCL/PLGA) membrane and glutaraldehyde (3.5% v/v) cross-linked chitosan/gelatin hydrogel was fabricated using two methods: electrospinning of the membrane onto the lyophilized hydrogel (BS-1) and membrane ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211416527
更新日期：2013-01-01 00:00:00
abstract：:An electronic sensor system for urinary bladder pressure monitoring requires an imbedding into a biocompatible, flexible, and liquid-impermeable material. Poly(dimethylsiloxane) (PDMS) was selected in the present set-up as packaging material because it fulfills the abovementioned requirements. However, the surface of ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328211422119
更新日期：2013-02-01 00:00:00
abstract：:A range of crystalline and nano-sized carbonate- and silicate-substituted hydroxyapatite has been successfully produced by using continuous hydrothermal flow synthesis technology. Ion-substituted calcium phosphates are better candidates for bone replacement applications (due to improved bioactivity) as compared to pha...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328212460289
更新日期：2013-09-01 00:00:00
abstract：:The objective of this study is to evaluate the osteoconductivity and bioactivity of the Bonelike graft in repairing surgical cystic bone defects. Bonelike is implanted in 11 patients, aged between 24 and 53 years with a mean age of 36 years, consisting of 5 men and 6 women. According to the standard follow up protocol...
journal_title：Journal of biomaterials applications
pub_type： 临床试验,杂志文章
doi：10.1177/0885328207078260
更新日期：2008-01-01 00:00:00
abstract：:The controlled release of insulin from a series of biodegradable hybrid hydrogel network containing dextran derivative of allyl isocyanate (dex-AI) and poly (D,L) lactide diacrylate macromer (PDLLAM) over a wide range of composition ratio was investigated. Laser confocal scanning microscope was used to understand the ...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1106/088532802024248
更新日期：2002-04-01 00:00:00
abstract：:Appropriate design of dialysis membranes requires correct values of structural parameters such as pore radius, surface porosity, water content and tortuosity. It is impossible, however, to find the pore radius of dialysis membranes using a mercury porosimeter or an electron microscope because the pores are only severa...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532828900400201
更新日期：1989-10-01 00:00:00
abstract：:Staphylococcus epidermidis plays a major role in capsular contractures of silicone breast implants. This in vitro study evaluates the antibacterial effect of copper on S. epidermidis in silicone implants. Specimens of a silicone material used for breast augmentation (Cu0) and specimens coated with different copper con...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328212441957
更新日期：2013-08-01 00:00:00
abstract：:In this work, gold embedded chitosan nanoparticles (Au@CS NPs) were fabricated by a one-pot method. The benzaldehyde-terminated poly[(2-methacryloyloxy) ethyl phosphorylcholine] (PMPC) was applied to modification of the gold doped chitosan nanoparticles. The obtained Au@CS-PMPC NPs had the diameter of 135 nm with a na...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328220952594
更新日期：2020-08-27 00:00:00
abstract：:Herb borneol is usually used in clinics for the treatment of central nervous system illness, for its ability of blood-brain barrier permeability, although its poor water solubility and poor bioavailability limit its clinical application to some degree. In this study, we developed a novel nanoparticle combining the ben...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328218799551
更新日期：2018-10-01 00:00:00
abstract：:The paper studies the coupling reaction by covalent bonding of acetylamino-2-sulfamoyl-1,3,4-thiodiazole (AcAA) on the poly(acrylic acid-costyrene) copolymer (PAcA-S) in homogeneous system, in the presence of dicyclohexylcarbodiimide (DCC) as activator. The influence of some factors on coupling efficiency (the drug/su...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532829200700105
更新日期：1992-07-01 00:00:00
abstract：:NiTi alloy is used as a biomaterial due to its pseudoelastic behaviour. It exhibits a great potential in dental and orthopedic applications where constant correcting loads are required. In order to use such materials, it is necessary to investigate the effect of the cyclic straining upon the transformation stresses an...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/088532829801200304
更新日期：1998-01-01 00:00:00
abstract：:We assess the effects of chemical processing, ethylene oxide sterilization, and threading on bone surface and mechanical properties of bovine undecalcified bone screws. In addition, we evaluate the possibility of manufacturing bone screws with predefined dimensions. Scanning electronic microscopic images show that che...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328208092110
更新日期：2009-03-01 00:00:00
abstract：:Excessive demineralization in osteoporotic bones impairs its self-regeneration potential following a defect/fracture and is of great concern among the aged population. In this context, implants with inherent osteogenic ability loaded with therapeutic ions like Strontium (Sr2+) may bring forth promising outcomes. Micro...
journal_title：Journal of biomaterials applications
pub_type： 杂志文章
doi：10.1177/0885328216647197
更新日期：2016-10-01 00:00:00
