Determination of Acid and Neutral Cannabinoids in Extracts of Different Strains of Cannabis sativa Using GC-FID.

Abstract:

:Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 - 0.19 µg/mL, and the limit of quantitation was 0.34 - 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).

journal_name

Planta Med

journal_title

Planta medica

authors

Ibrahim EA,Gul W,Gul SW,Stamper BJ,Hadad GM,Abdel Salam RA,Ibrahim AK,Ahmed SA,Chandra S,Lata H,Radwan MM,ElSohly MA

doi

10.1055/s-0043-124088

subject

Has Abstract

pub_date

2018-03-01 00:00:00

pages

250-259

issue

4

eissn

0032-0943

issn

1439-0221

journal_volume

84

pub_type

杂志文章
  • Activity on the central nervous system of Himanthalia elongata.

    abstract::We report the effects of a fraction (F1) obtained from Himanthalia elongata on spontaneous locomotor activity, hypermotility, motor coordination, rectal temperature, pentobarbitone-induced hypnosis, pentylenetetrazole-induced convulsions and analgesic activity in the writhing and hot-plate tests. This fraction caused ...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-959655

    authors: Anca JM,Lamela M,Calleja JM

    更新日期:1993-06-01 00:00:00

  • Flavonoids and a sesquiterpene lactone from Tanacetum microphyllum inhibit anti-inflammatory mediators in LPS-stimulated mouse peritoneal macrophages.

    abstract::In our ongoing research into anti-inflammatory compounds from Tanacetum microphyllum, four naturally occurring flavonoids (santin, ermanin, centaureidin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol) and one sesquiterpene lactone (hydroxyachillin) isolated from this plant, were evaluated as potential inhibit...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2004-815452

    authors: Abad MJ,Bermejo P,Alvarez M,Guerra JA,Silván AM,Villar AM

    更新日期:2004-01-01 00:00:00

  • Uterine effects of the phytoestrogen 6-(1,1-dimethylallyl)naringenin in rats.

    abstract::Phytoestrogens are discussed as candidate substances to treat symptoms related to estrogen deficiency. In in vitro experiments, the naturally occurring flavonoid 6-(1,1-dimethylallyl)naringenin (6-DMAN) emerged as one of the most potent phytoestrogenic substances. 6-DMAN is not as well characterized as other flavonoid...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2004-827178

    authors: Zierau O,Geis RB,Tischer S,Schwab P,Metz P,Vollmer G

    更新日期:2004-07-01 00:00:00

  • Two new secoiridoid glycosides from Gentiana algida.

    abstract::Two new acylated secoiridoid glycosides were isolated from the aqueous acetone extract of the whole herb of Gentiana algida. The structures of these new products were established by spectral and chemical methods as 6'-(2,3-dihydroxybenzoyl)sweroside and 6'-(2,3-dihydroxybenzoyl)swertiamarin, respectively. ...

    journal_title:Planta medica

    pub_type: 信件

    doi:10.1055/s-2006-957770

    authors: Tan RX,Hu J,Dong LD,Wolfender JL,Hostettmann K

    更新日期:1997-12-01 00:00:00

  • Effects of Evodia rutaecarpa and rutaecarpine on the pharmacokinetics of caffeine in rats.

    abstract::To assess possible herb-drug interactions, rutaecarpine (an herbal ingredient of Evodia rutaecarpa; 25 mg/kg/day, p. o.), the ethanol extract of Evodia rutaecarpa (1 g/kg/day, p. o.), and an herbal preparation of Evodia rutaecarpa (Wu-Chu-Yu-Tang; 1 g/kg/day) were individually pretreated daily for three consecutive da...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2005-871270

    authors: Tsai TH,Chang CH,Lin LC

    更新日期:2005-07-01 00:00:00

  • Cytotoxic alkaloids from the roots of Tylophora atrofolliculata.

    abstract::Four new phenanthroindolizidine alkaloids, tylophoridicines C-F, together with three known ones, R-(+)-deoxytylophorinidine, tylophorinine and tylophorinidine, were isolated from the roots of Tylophora atrofolliculata. The structures were determined on the basis of spectral evidence. These alkaloids exhibited cytotoxi...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2004-818973

    authors: Huang X,Gao S,Fan L,Yu S,Liang X

    更新日期:2004-05-01 00:00:00

  • Somatic embryogenesis and rhizogenesis of tissue cultures of two genotypes of Papaver somniferum: relationships to alkaloids production.

    abstract::PAPAVER SOMNIFERUM L. tissue cultures, issued from various explants (cotyledons, hypocotyls, roots) derived from plantlets belonging to two genotypes, were established on LS solid medium containing growth regulators (NAA, Kin) in various combinations. Hypocotyls and roots were found to be interesting explants to obtai...

    journal_title:Planta medica

    pub_type: 信件

    doi:10.1055/s-2006-960457

    authors: Laurain-Mattar D,Gillet-Manceau F,Buchon L,Nabha S,Fliniaux MA,Jacquin-Bubreuil A

    更新日期:1999-03-01 00:00:00

  • Single-Lab Validation for Determination of Kavalactones and Flavokavains in Piper methysticum (Kava).

    abstract::Piper methysticum (Kava) is a plant whose roots are used in the preparation of traditional beverages with spiritual, medicinal, and social importance for the Pacific Islanders. Kava is also sold as a herbal supplement or recreational beverage consumed for its mild inebriating effect in Europe and North America. With a...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/a-0637-2400

    authors: Liu Y,Lund JA,Murch SJ,Brown PN

    更新日期:2018-11-01 00:00:00

  • Inhibition of butyrylcholinesterase activity by some isoquinoline alkaloids.

    abstract::The inhibition of butyrylcholinesterase activity in human serum by protoberberine, benzophenanthridine and aporphine alkaloids as well as four model compounds has been studied. The mechanism of the inhibition is discussed on the basis of the different types of interaction of these compounds with butyrylcholinesterase ...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2007-969916

    authors: Ulrichová J,Walterová D,Preininger V,Simánek V

    更新日期:1983-07-01 00:00:00

  • Spasmolytic and antinociceptive activities of ursolic acid and acacetin identified in Agastache mexicana.

    abstract::Agastache mexicana is a plant in high demand that has long been used in Mexican folk medicine to treat anxiety, insomnia, and stomachache, among other afflictions. Ursolic acid and acacetin were isolated and identified as two possible active compounds of A. mexicana aerial parts. An antinociceptive response was demons...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-0031-1298416

    authors: González-Trujano ME,Ventura-Martínez R,Chávez M,Díaz-Reval I,Pellicer F

    更新日期:2012-05-01 00:00:00

  • Cratoxylum formosum Extract Protects against Amyloid-Beta Toxicity in a Caenorhabditis elegans Model of Alzheimer's Disease.

    abstract::Amyloid-β, one of the hallmarks of Alzheimer's disease, is toxic to neurons and causes cell death in the brain. Oxidative stress is known to play an important role in Alzheimer's disease, and there is strong evidence linking oxidative stress to amyloid-β. The herbal plant "Tiew kon" (Cratoxylum formosum ssp. pruniflor...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-0041-111621

    authors: Keowkase R,Weerapreeyakul N

    更新日期:2016-04-01 00:00:00

  • Anti-asthmatic effects of Perilla seed oil in the guinea pig in vitro and in vivo.

    abstract::The aim of this study was to investigate the anti-asthmatic effects of Perilla seed oil in vitro and in vivo in sensitized guinea pigs. Aerosolized antigen caused an immediate bronchoconstriction. Perilla seed oil per os inhibited the increase in lung resistance and the decrease in dynamic lung compliance in a dose-de...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-957062

    authors: Deng YM,Xie QM,Zhang SJ,Yao HY,Zhang H

    更新日期:2007-01-01 00:00:00

  • [Germacranolides, Guaianolides, and Xanthanolides from the Flowers of Arnica mollis and an X-Ray Structure Analysis of Baileyin Acetate].

    abstract::The flowers of ARNICA MOLLIS afforded, in addition to baileyin ( 1A), baileyin acetate ( 1B), xanthalongin ( 3), and 2-deacetoxy-11alpha,13-dihydroxanthuminol ( 6), the new 1,5- CIS-guaianolides arnimollin acetate ( 2A) and arnimollin tiglinate ( 2A) and the new xanthanolides 11beta3,13-dihydroxanthalongin ( 4) and 11...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-960898

    authors: Marcinek-Hüpen-Bestendonk C,Willuhn G,Steigel A,Wendisch D,Middelhauve B,Wiebcke M,Mootz D

    更新日期:1990-02-01 00:00:00

  • Cnidicin, a coumarin, from the root of Angelica koreana, inhibits the degranulation of mast cell and the NO generation in RAW 264.7 cells.

    abstract::Cnidicin (1) and five related coumarins were isolated from the root extract of Angelica koreana (Umbelliferae) as active principles responsible for the inhibitory effect on the degranulation process of cultured mast cells. Cnidicin (1) demonstrated a significant inhibition upon the release of beta-hexosaminidase from ...

    journal_title:Planta medica

    pub_type: 信件

    doi:10.1055/s-2001-11509

    authors: Ryu SY,Kou NY,Choi HS,Ryu H,Kim TS,Kim KM

    更新日期:2001-03-01 00:00:00

  • Biosynthetic Studies on Acetosellin and Structure Elucidation of a New Acetosellin Derivative.

    abstract::Natural products from fungi, especially Ascomycota, play a major role in therapy and drug discovery. Fungal strains originating from marine habitats offer a new avenue for finding unusual molecular skeletons. Here, the marine-derived fungus Epicoccum nigrum (strain 749) was found to produce the azaphilonoid compounds ...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-0042-124493

    authors: Hufendiek P,Stölben SSM,Kehraus S,Merten N,Harms H,Crüsemann M,Arslan I,Gütschow M,Schneider T,König GM

    更新日期:2017-08-01 00:00:00

  • Plant-derived leading compounds for chemotherapy of human immunodeficiency virus (HIV) infection - an update (1998 - 2007).

    abstract::The high number of citations of the previous review on anti-HIV compounds from plants published in 1998 in Planta Medica indicates the importance of natural products research in the battle against HIV. Therefore, we have decided to write an update of our previous review paper, this time covering the time span 1998 - 2...

    journal_title:Planta medica

    pub_type: 杂志文章,评审

    doi:10.1055/s-2008-1081314

    authors: Cos P,Maes L,Vlietinck A,Pieters L

    更新日期:2008-09-01 00:00:00

  • Improved method for the determination of oxindole alkaloids in Uncaria tomentosa by high performance liquid chromatography.

    abstract::This Paper describes an improved HPLC method for the determination of pentacyclic oxindole alkaloids in Uncaria tomentosa (Cat's Claw). Six of the isomeric compounds could be baseline separated at room temperature within less than 30 min by using 3 microm C-18 column material and a mobile phase consisting of 10 mM pho...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2001-15824

    authors: Ganzera M,Muhammad I,Khan RA,Khan IA

    更新日期:2001-07-01 00:00:00

  • 6-Benzylaminopurine: a plant derived cytokinin inducing positive inotropism by P2-purinoceptors.

    abstract::Positive inotropic effects induced by 6-benzylaminopurine (6-BAP), kinetin and zeatin were studied in rat atria. The potency order observed was 6-BAP > or = kinetin > zeatin. Suramin, a P2-purinoceptor antagonist, inhibited the positive effect of 6-BAP suggesting the involvement of P2-purinoceptors in the positive eff...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-1999-13987

    authors: Froldi G,Gallo U,Ragazzi E,Caparrotta L

    更新日期:1999-04-01 00:00:00

  • Phytochemical analysis and cytotoxicity towards multidrug-resistant leukemia cells of essential oils derived from Lebanese medicinal plants.

    abstract::Juniperus excelsa fruit essential oil as well as J. oxycedrus, Cedrus libani, and Pinus pinea wood essential oils have been obtained with yields between 2.2 ± 0.3 % to 3.4 ± 0.5 % and analyzed by gas chromatography. Sesquiterpenes mainly characterized C. libani and J. oxycedrus essential oils, while in P. pinea and J....

    journal_title:Planta medica

    pub_type: 信件

    doi:10.1055/s-0032-1327896

    authors: Saab AM,Guerrini A,Sacchetti G,Maietti S,Zeino M,Arend J,Gambari R,Bernardi F,Efferth T

    更新日期:2012-12-01 00:00:00

  • High performance liquid chromatography in phytochemical analysis of Eurycoma longifolia.

    abstract::An analytical method using HPLC with UV detection was developed to investigate the quassinoid content of Eurycoma longifolia Jack (Simaroubaceae) collected from various sources. Eurycomanone (1), longilactone (2), 14,15beta-dihydroxyklaineanone (3), 15beta-acetyl-14-hydroxyklaineanone (4), 6alpha-hydroxyeurycomalacton...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-957570

    authors: Chan KL,Choo CY,Morita H,Itokawa H

    更新日期:1998-12-01 00:00:00

  • Studies on the endocrine effects of the contents of Cimicifuga racemosa:.

    abstract::Endocrine activity can be demonstrated for components of the rhizome of CIMICIFUGA RACEMOSA using ovariectomized rats as experimental model. A selective reduction of the serum concentration of the pituitary luteinizing hormone (LH) takes place upon application of an extract. The active principle can be concentrated by...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2007-969390

    authors: Jarry H,Harnischfeger G

    更新日期:1985-02-01 00:00:00

  • Isoliquiritigenin inhibits cell proliferation and induces apoptosis in human hepatoma cells.

    abstract::Isoliquiritigenin (4,2',4'-trihydroxychalcone, ISL) is a natural pigment with a simple chalcone structure. In this study, we report the ISL-induced inhibition on the growth of human hepatoma cells (Hep G2) for the first time. The cell growth inhibition achieved by ISL treatment resulted in programmed cell death in a c...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2005-837779

    authors: Hsu YL,Kuo PL,Lin LT,Lin CC

    更新日期:2005-02-01 00:00:00

  • Procyanidines from Vitis vinifera seeds protect rabbit heart from ischemia/reperfusion injury: antioxidant intervention and/or iron and copper sequestering ability.

    abstract::An isolated rabbit heart Langendorff preparation paced electrically was used to evaluate the effects of a highly purified, high molecular weight fraction of oligomeric procyanidines isolated from Vitis vinifera seeds on myocardial reperfusion injury after 40 minutes of low flow (1 ml/min) ischemia. Infusion of the hea...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-957956

    authors: Maffei Facinó R,Carini M,Aldini G,Berti F,Rossoni G,Bombardelli E,Morazzoni P

    更新日期:1996-12-01 00:00:00

  • Determination of Final Steps in Biosyntheses of Essential Oil Components in Perilla frutescens.

    abstract::Biosynthetic pathways of the essential oil components in PERILLA FRUTESCENS were examined by tracer experiments using [ (14)C]-labeled sucrose on two chemotypes: perillaketone (PK) and phenylpropanoid (PP-EM) types. The results of the experiments indicated that perillaketone is not converted from isoegomaketone but is...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-961996

    authors: Nishizawa A,Honda G,Tabata M

    更新日期:1989-06-01 00:00:00

  • Plant-derived leading compounds for chemotherapy of human immunodeficiency virus (HIV) infection.

    abstract::Many compounds of plant origin have been identified that inhibit different stages in the replication cycle of human immunodeficiency virus (HIV): 1) virus adsorption: chromone alkaloids (schumannificine), isoquinoline alkaloids (michellamines), sulphated polysaccharides and polyphenolics, flavonoids, coumarins (glycoc...

    journal_title:Planta medica

    pub_type: 杂志文章,评审

    doi:10.1055/s-2006-957384

    authors: Vlietinck AJ,De Bruyne T,Apers S,Pieters LA

    更新日期:1998-03-01 00:00:00

  • Effect on the wound healing process and in vitro cell proliferation by the medicinal Mexican plant Ageratina pichinchensis.

    abstract::The species Ageratina pichinchensis (Asteraceae) has been used for a long time in Mexican traditional medicine for the treatment of different skin conditions and injuries. In this study, the healing capacity of the plant extracts obtained was evaluated and, in order to understand the mechanism of healing, we also anal...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-0030-1250743

    authors: Romero-Cerecero O,Zamilpa-Álvarez A,Ramos-Mora A,Alonso-Cortés D,Jiménez-Ferrer JE,Huerta-Reyes ME,Tortoriello J

    更新日期:2011-07-01 00:00:00

  • Barbaloin in aloe species.

    abstract::Barbaloin levels in the exudates from the leaves of 68 species of ALOE in the Kew collection have been determined by light absorption at 375 nm following separation by HPLC. The exudates from most species contained between 10-20% although a few concentrations of around 30% were found. The level in the leaf was usually...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2006-962735

    authors: Groom QJ,Reynolds T

    更新日期:1987-08-01 00:00:00

  • A new strain of Claviceps purpurea accumulating tetracyclic clavine alkaloids.

    abstract::A new strain of Claviceps was isolated from a blokked mutant of Claviceps purpurea. This strain accumulates substantial amounts of clavine alkaloids (2 g/l). The alkaloid fraction is composed of chanoclavine-I ( approximately 10%) and a mixture of agroclavine/elymoclavine (90%). Most suitable for alkaloid production i...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2007-971231

    authors: Schumann B,Erge D,Maier W,Gröger D

    更新日期:1982-05-01 00:00:00

  • Shogaols from Zingiber officinale protect IMR32 human neuroblastoma and normal human umbilical vein endothelial cells from beta-amyloid(25-35) insult.

    abstract::From the rhizome of Zingiber officinale L. (Zingiberaceae), four shogaols that protect IMR32 human neuroblastoma and normal human umbilical vein endothelial cells from beta-amyloid(25 - 35) insult at EC50 = 4.5 - 81 microM were isolated. The efficacy of cell protection from beta-amyloid(25 - 35) insult by these shogao...

    journal_title:Planta medica

    pub_type: 信件

    doi:10.1055/s-2002-26757

    authors: Kim DS,Kim DS,Oppel MN

    更新日期:2002-04-01 00:00:00

  • Stabilization of growth factors relevant to wound healing by a plant cell wall biomaterial.

    abstract::Stabilization of growth factors in a wound environment is critical to the wound healing process. Here we report on the stabilization of key growth factors by a unique plant cell wall biomaterial. MicroSheets are clear cell wall fragments isolated from the non-living water storage cells in the pulp or inner gel of Aloe...

    journal_title:Planta medica

    pub_type: 杂志文章

    doi:10.1055/s-2007-990225

    authors: Ni Y,Turner D,Yates K,Tizard I

    更新日期:2007-10-01 00:00:00