Abstract:
:Cannabis (Cannabis sativa L.) is an annual herbaceous plant that belongs to the family Cannabaceae. Trans-Δ9-tetrahydrocannabinol (Δ9-THC) and cannabidiol (CBD) are the two major phytocannabinoids accounting for over 40% of the cannabis plant extracts, depending on the variety. At the University of Mississippi, different strains of C. sativa, with different concentration ratios of CBD and Δ9-THC, have been tissue cultured via micropropagation and cultivated. A GC-FID method has been developed and validated for the qualitative and quantitative analysis of acid and neutral cannabinoids in C. sativa extracts. The method involves trimethyl silyl derivatization of the extracts. These cannabinoids include tetrahydrocannabivarian, CBD, cannabichromene, trans-Δ8-tetrahydrocannabinol, Δ9-THC, cannabigerol, cannabinol, cannabidiolic acid, cannabigerolic acid, and Δ9-tetrahydrocannabinolic acid-A. The concentration-response relationship of the method indicated a linear relationship between the concentration and peak area ratio with R2 > 0.999 for all 10 cannabinoids. The precision and accuracy of the method were found to be ≤ 15% and ± 5%, respectively. The limit of detection range was 0.11 - 0.19 µg/mL, and the limit of quantitation was 0.34 - 0.56 µg/mL for all 10 cannabinoids. The developed method is simple, sensitive, reproducible, and suitable for the detection and quantitation of acidic and neutral cannabinoids in different extracts of cannabis varieties. The method was applied to the analysis of these cannabinoids in different parts of the micropropagated cannabis plants (buds, leaves, roots, and stems).
journal_name
Planta Medjournal_title
Planta medicaauthors
Ibrahim EA,Gul W,Gul SW,Stamper BJ,Hadad GM,Abdel Salam RA,Ibrahim AK,Ahmed SA,Chandra S,Lata H,Radwan MM,ElSohly MAdoi
10.1055/s-0043-124088subject
Has Abstractpub_date
2018-03-01 00:00:00pages
250-259issue
4eissn
0032-0943issn
1439-0221journal_volume
84pub_type
杂志文章相关文献
PLANTA MEDICA文献大全abstract::We report the effects of a fraction (F1) obtained from Himanthalia elongata on spontaneous locomotor activity, hypermotility, motor coordination, rectal temperature, pentobarbitone-induced hypnosis, pentylenetetrazole-induced convulsions and analgesic activity in the writhing and hot-plate tests. This fraction caused ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-959655
更新日期:1993-06-01 00:00:00
abstract::In our ongoing research into anti-inflammatory compounds from Tanacetum microphyllum, four naturally occurring flavonoids (santin, ermanin, centaureidin and 5,3'-dihydroxy-4'-methoxy-7-methoxycarbonylflavonol) and one sesquiterpene lactone (hydroxyachillin) isolated from this plant, were evaluated as potential inhibit...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-815452
更新日期:2004-01-01 00:00:00
abstract::Phytoestrogens are discussed as candidate substances to treat symptoms related to estrogen deficiency. In in vitro experiments, the naturally occurring flavonoid 6-(1,1-dimethylallyl)naringenin (6-DMAN) emerged as one of the most potent phytoestrogenic substances. 6-DMAN is not as well characterized as other flavonoid...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-827178
更新日期:2004-07-01 00:00:00
abstract::Two new acylated secoiridoid glycosides were isolated from the aqueous acetone extract of the whole herb of Gentiana algida. The structures of these new products were established by spectral and chemical methods as 6'-(2,3-dihydroxybenzoyl)sweroside and 6'-(2,3-dihydroxybenzoyl)swertiamarin, respectively. ...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2006-957770
更新日期:1997-12-01 00:00:00
abstract::To assess possible herb-drug interactions, rutaecarpine (an herbal ingredient of Evodia rutaecarpa; 25 mg/kg/day, p. o.), the ethanol extract of Evodia rutaecarpa (1 g/kg/day, p. o.), and an herbal preparation of Evodia rutaecarpa (Wu-Chu-Yu-Tang; 1 g/kg/day) were individually pretreated daily for three consecutive da...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2005-871270
更新日期:2005-07-01 00:00:00
abstract::Four new phenanthroindolizidine alkaloids, tylophoridicines C-F, together with three known ones, R-(+)-deoxytylophorinidine, tylophorinine and tylophorinidine, were isolated from the roots of Tylophora atrofolliculata. The structures were determined on the basis of spectral evidence. These alkaloids exhibited cytotoxi...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2004-818973
更新日期:2004-05-01 00:00:00
abstract::PAPAVER SOMNIFERUM L. tissue cultures, issued from various explants (cotyledons, hypocotyls, roots) derived from plantlets belonging to two genotypes, were established on LS solid medium containing growth regulators (NAA, Kin) in various combinations. Hypocotyls and roots were found to be interesting explants to obtai...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2006-960457
更新日期:1999-03-01 00:00:00
abstract::Piper methysticum (Kava) is a plant whose roots are used in the preparation of traditional beverages with spiritual, medicinal, and social importance for the Pacific Islanders. Kava is also sold as a herbal supplement or recreational beverage consumed for its mild inebriating effect in Europe and North America. With a...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/a-0637-2400
更新日期:2018-11-01 00:00:00
abstract::The inhibition of butyrylcholinesterase activity in human serum by protoberberine, benzophenanthridine and aporphine alkaloids as well as four model compounds has been studied. The mechanism of the inhibition is discussed on the basis of the different types of interaction of these compounds with butyrylcholinesterase ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969916
更新日期:1983-07-01 00:00:00
abstract::Agastache mexicana is a plant in high demand that has long been used in Mexican folk medicine to treat anxiety, insomnia, and stomachache, among other afflictions. Ursolic acid and acacetin were isolated and identified as two possible active compounds of A. mexicana aerial parts. An antinociceptive response was demons...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0031-1298416
更新日期:2012-05-01 00:00:00
abstract::Amyloid-β, one of the hallmarks of Alzheimer's disease, is toxic to neurons and causes cell death in the brain. Oxidative stress is known to play an important role in Alzheimer's disease, and there is strong evidence linking oxidative stress to amyloid-β. The herbal plant "Tiew kon" (Cratoxylum formosum ssp. pruniflor...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0041-111621
更新日期:2016-04-01 00:00:00
abstract::The aim of this study was to investigate the anti-asthmatic effects of Perilla seed oil in vitro and in vivo in sensitized guinea pigs. Aerosolized antigen caused an immediate bronchoconstriction. Perilla seed oil per os inhibited the increase in lung resistance and the decrease in dynamic lung compliance in a dose-de...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957062
更新日期:2007-01-01 00:00:00
abstract::The flowers of ARNICA MOLLIS afforded, in addition to baileyin ( 1A), baileyin acetate ( 1B), xanthalongin ( 3), and 2-deacetoxy-11alpha,13-dihydroxanthuminol ( 6), the new 1,5- CIS-guaianolides arnimollin acetate ( 2A) and arnimollin tiglinate ( 2A) and the new xanthanolides 11beta3,13-dihydroxanthalongin ( 4) and 11...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-960898
更新日期:1990-02-01 00:00:00
abstract::Cnidicin (1) and five related coumarins were isolated from the root extract of Angelica koreana (Umbelliferae) as active principles responsible for the inhibitory effect on the degranulation process of cultured mast cells. Cnidicin (1) demonstrated a significant inhibition upon the release of beta-hexosaminidase from ...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2001-11509
更新日期:2001-03-01 00:00:00
abstract::Natural products from fungi, especially Ascomycota, play a major role in therapy and drug discovery. Fungal strains originating from marine habitats offer a new avenue for finding unusual molecular skeletons. Here, the marine-derived fungus Epicoccum nigrum (strain 749) was found to produce the azaphilonoid compounds ...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0042-124493
更新日期:2017-08-01 00:00:00
abstract::The high number of citations of the previous review on anti-HIV compounds from plants published in 1998 in Planta Medica indicates the importance of natural products research in the battle against HIV. Therefore, we have decided to write an update of our previous review paper, this time covering the time span 1998 - 2...
journal_title:Planta medica
pub_type: 杂志文章,评审
doi:10.1055/s-2008-1081314
更新日期:2008-09-01 00:00:00
abstract::This Paper describes an improved HPLC method for the determination of pentacyclic oxindole alkaloids in Uncaria tomentosa (Cat's Claw). Six of the isomeric compounds could be baseline separated at room temperature within less than 30 min by using 3 microm C-18 column material and a mobile phase consisting of 10 mM pho...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2001-15824
更新日期:2001-07-01 00:00:00
abstract::Positive inotropic effects induced by 6-benzylaminopurine (6-BAP), kinetin and zeatin were studied in rat atria. The potency order observed was 6-BAP > or = kinetin > zeatin. Suramin, a P2-purinoceptor antagonist, inhibited the positive effect of 6-BAP suggesting the involvement of P2-purinoceptors in the positive eff...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-1999-13987
更新日期:1999-04-01 00:00:00
abstract::Juniperus excelsa fruit essential oil as well as J. oxycedrus, Cedrus libani, and Pinus pinea wood essential oils have been obtained with yields between 2.2 ± 0.3 % to 3.4 ± 0.5 % and analyzed by gas chromatography. Sesquiterpenes mainly characterized C. libani and J. oxycedrus essential oils, while in P. pinea and J....
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-0032-1327896
更新日期:2012-12-01 00:00:00
abstract::An analytical method using HPLC with UV detection was developed to investigate the quassinoid content of Eurycoma longifolia Jack (Simaroubaceae) collected from various sources. Eurycomanone (1), longilactone (2), 14,15beta-dihydroxyklaineanone (3), 15beta-acetyl-14-hydroxyklaineanone (4), 6alpha-hydroxyeurycomalacton...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957570
更新日期:1998-12-01 00:00:00
abstract::Endocrine activity can be demonstrated for components of the rhizome of CIMICIFUGA RACEMOSA using ovariectomized rats as experimental model. A selective reduction of the serum concentration of the pituitary luteinizing hormone (LH) takes place upon application of an extract. The active principle can be concentrated by...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-969390
更新日期:1985-02-01 00:00:00
abstract::Isoliquiritigenin (4,2',4'-trihydroxychalcone, ISL) is a natural pigment with a simple chalcone structure. In this study, we report the ISL-induced inhibition on the growth of human hepatoma cells (Hep G2) for the first time. The cell growth inhibition achieved by ISL treatment resulted in programmed cell death in a c...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2005-837779
更新日期:2005-02-01 00:00:00
abstract::An isolated rabbit heart Langendorff preparation paced electrically was used to evaluate the effects of a highly purified, high molecular weight fraction of oligomeric procyanidines isolated from Vitis vinifera seeds on myocardial reperfusion injury after 40 minutes of low flow (1 ml/min) ischemia. Infusion of the hea...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-957956
更新日期:1996-12-01 00:00:00
abstract::Biosynthetic pathways of the essential oil components in PERILLA FRUTESCENS were examined by tracer experiments using [ (14)C]-labeled sucrose on two chemotypes: perillaketone (PK) and phenylpropanoid (PP-EM) types. The results of the experiments indicated that perillaketone is not converted from isoegomaketone but is...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-961996
更新日期:1989-06-01 00:00:00
abstract::Many compounds of plant origin have been identified that inhibit different stages in the replication cycle of human immunodeficiency virus (HIV): 1) virus adsorption: chromone alkaloids (schumannificine), isoquinoline alkaloids (michellamines), sulphated polysaccharides and polyphenolics, flavonoids, coumarins (glycoc...
journal_title:Planta medica
pub_type: 杂志文章,评审
doi:10.1055/s-2006-957384
更新日期:1998-03-01 00:00:00
abstract::The species Ageratina pichinchensis (Asteraceae) has been used for a long time in Mexican traditional medicine for the treatment of different skin conditions and injuries. In this study, the healing capacity of the plant extracts obtained was evaluated and, in order to understand the mechanism of healing, we also anal...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-0030-1250743
更新日期:2011-07-01 00:00:00
abstract::Barbaloin levels in the exudates from the leaves of 68 species of ALOE in the Kew collection have been determined by light absorption at 375 nm following separation by HPLC. The exudates from most species contained between 10-20% although a few concentrations of around 30% were found. The level in the leaf was usually...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2006-962735
更新日期:1987-08-01 00:00:00
abstract::A new strain of Claviceps was isolated from a blokked mutant of Claviceps purpurea. This strain accumulates substantial amounts of clavine alkaloids (2 g/l). The alkaloid fraction is composed of chanoclavine-I ( approximately 10%) and a mixture of agroclavine/elymoclavine (90%). Most suitable for alkaloid production i...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-971231
更新日期:1982-05-01 00:00:00
abstract::From the rhizome of Zingiber officinale L. (Zingiberaceae), four shogaols that protect IMR32 human neuroblastoma and normal human umbilical vein endothelial cells from beta-amyloid(25 - 35) insult at EC50 = 4.5 - 81 microM were isolated. The efficacy of cell protection from beta-amyloid(25 - 35) insult by these shogao...
journal_title:Planta medica
pub_type: 信件
doi:10.1055/s-2002-26757
更新日期:2002-04-01 00:00:00
abstract::Stabilization of growth factors in a wound environment is critical to the wound healing process. Here we report on the stabilization of key growth factors by a unique plant cell wall biomaterial. MicroSheets are clear cell wall fragments isolated from the non-living water storage cells in the pulp or inner gel of Aloe...
journal_title:Planta medica
pub_type: 杂志文章
doi:10.1055/s-2007-990225
更新日期:2007-10-01 00:00:00