Abstract:
:Patients with the luminal B subtype of breast cancer exhibit a poor prognosis, high metastatic risk and high incidence of chemotherapy resistance. Luminal B breast cancer is sub-classified into B1 and B2. The pathophysiological mechanism of luminal B2 breast cancer (LB2BC) progression has yet to be characterized. Therefore, the present study aimed to identify the genes involved in the pathogenesis of LB2BC. The data of 117 LB2BC expression profiles were downloaded from The Cancer Genome Atlas (TCGA) and differentially expressed genes (DEGs) were identified by comparison with non-tumor tissue expression profiles. Gene Ontology enrichment analysis, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis and protein-protein interaction (PPI) networks were used to obtain insight into the functions of DEGs. Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) analysis was performed to validate the expression level of DEGs in tissue samples. A total of 2,251 DEGs, including 759 upregulated and 1,492 downregulated genes, were identified between LB2BC and non-tumor tissues. The top 15 upregulated and downregulated genes were used to construct a PPI network: Epidermal growth factor receptor (EGFR), fibronectin-1 (FN1) and Polo-like kinase-1 had the highest connectivity degrees. KEGG analysis identified that DEGs were most significantly enriched in 'focal adhesion', 'pathways in cancer' and 'ECM-receptor interaction' pathways. The results of RT-qPCR demonstrated that EGFR was significantly downregulated in LB2BC, whereas FN1 was significantly upregulated, whereas neurotrophic receptor tyrosine kinase 2 (NTRK2) trended towards downregulation. In conclusion, the DEGs identified in the present study, including NTRK2, FN1 and EGFR, may serve pivotal roles in the tumorigenesis of LB2BC by affecting the 'focal adhesion', 'pathways in cancer' and 'ECM-receptor interaction' KEGG pathways.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Wang J,Du Q,Li Cdoi
10.3892/ol.2017.7256subject
Has Abstractpub_date
2017-12-01 00:00:00pages
7880-7888issue
6eissn
1792-1074issn
1792-1082pii
OL-0-0-7256journal_volume
14pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Xeroderma pigmentosum variant (XP-V) is a subtype of xeroderma pigmentosum (XP) disease with typical pigmentation and types of cancer in the oral maxillofacial and other sun-exposed regions. Few factors of tumor proneness in XP-V have been completely elucidated with the exception of the POLH [which encodes DNA polymer...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1604
更新日期:2013-12-01 00:00:00
abstract::Pneumocystis jiroveci pneumonia (PCP) has long been recognized as a cause of mortality in immuno-compromised populations, including those with advanced lung cancer. Although Pneumocystis colonization has only recently been described due to the development of more sensitive molecular techniques, including polymerase ch...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.1052
更新日期:2013-02-01 00:00:00
abstract::Previous studies have shown that the activation of the β2 adrenergic receptor (ADRB2) can stimulate several signaling pathways that promote tumor growth and metastasis. β-adrenergic antagonism may have a beneficial role in cancer treatment; however, little is known about the effect of ADRB2 inhibition on the growth of...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8847
更新日期:2018-08-01 00:00:00
abstract::Breast cancer is a highly heterogeneous disease at the molecular level and >90% of mortalities are due to metastasis and its associated complications. The present study determined the impact of molecular subtypes on metastatic behavior and overall survival (OS) of patients with metastatic breast cancer. The influence ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11948
更新日期:2020-10-01 00:00:00
abstract::Esophageal cancer staging is important for the treatment of esophageal cancer. Endoscopic ultrasonography (EUS) is a common diagnostic tool for esophageal cancer prior to surgery. However, EUS is unable to accurately discriminate the N-staging of lymph nodes. In order to distinguish an optimized standard for malignant...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9716
更新日期:2019-01-01 00:00:00
abstract::Although previous studies have demonstrated that dysregulation of microRNA (miR)-126 is associated with the progression of several types of cancer, including lung cancer, the relationship between miR-126 and lung cancer metastasis remains unclear. SPC-A1 lung cancer cells were transfected with miR-126 mimic and negati...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8207
更新日期:2018-05-01 00:00:00
abstract::Metastasis negatively affects the prognosis of hepatocellular carcinoma (HCC). In the present study, niclosamide, which is known to suppress the proliferation of HCC cells, was investigated for possible suppressant effects on the migration of HCC cells. HLF and PLC/PRF/5 HCC cells were cultured in the presence of nicl...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3789
更新日期:2015-12-01 00:00:00
abstract::Previous studies have demonstrated an association between human papillomavirus (HPV) and mutations in the epidermal growth factor receptor (EGFR) gene in lung cancer patients; however, few studies have investigated this association in advanced lung adenocarcinoma patients undergoing gefitinib treatment. The present st...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4847
更新日期:2016-09-01 00:00:00
abstract::Effective therapies are limited for pancreatic cancer, particularly for those with distant tumour metastases. Therefore, more individualised drug screening is urgently required. Next-generation sequencing (NGS) is a powerful tool to investigate the genomic landscape of patients and the mechanism of drug response, whic...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9876
更新日期:2019-02-01 00:00:00
abstract::Morphological response is considered an improved surrogate to the Response Evaluation Criteria in Solid Tumors (RECIST) model with regard to predicting the prognosis for patients with colorectal liver metastases. However, its use as a decision-making tool for surgical intervention has not been examined. The present st...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6338
更新日期:2017-08-01 00:00:00
abstract::Low-grade myofibroblastic sarcoma (LGMS) is a distinct mesenchymal myofibroblastic malignancy. The tumor may occur at a variety of sites, but is particularly associated with the head and neck. Of the two maxillary sarcomas that were analyzed in the present study, one was misdiagnosed as an inflammatory myofibroblastic...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2790
更新日期:2015-02-01 00:00:00
abstract::The aim of the present study was to investigate the association between the expression of chemokine receptors CCR7 and CXCR4 and vascular endothelial growth factor (VEGF)-C and the lymph node metastasis of pancreatic ductal adenocarcinoma (PDAC). The mRNA transcription levels of CCR7, CXCR4 and VEGF-C were measured in...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1261
更新日期:2013-05-01 00:00:00
abstract::The present study aimed to explore the clinical efficacy and safety of hydromorphone combined with sufentanil in patient-controlled intravenous analgesia (PCIA) for patients with hepatocellular carcinoma (HCC) and its effect on serum immune factors in serum. Data from 385 patients with HCC, admitted to the Hunan Provi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12159
更新日期:2020-12-01 00:00:00
abstract::Cyclin-dependent kinase inhibitor 2A (p16INK4a) protein is a surrogate immunohistochemical marker of human papillomavirus infection in oropharynx squamous cell carcinoma (OPSCC). However, the effects of p16INK4a in non-OPSCC require additional analysis. In addition, major gaps remain in the literature, including small...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9353
更新日期:2018-11-01 00:00:00
abstract::Leptomeningeal metastasis (LM) is increasingly common in patients with non-small cell lung cancer (NSCLC) due to improved treatment, and ultimately, prolonged patient survival. The current study is a pooled analysis that evaluated intrathecal chemotherapy (ITC) as a treatment for NSCLC patients with LM. The PUBMED, OV...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4783
更新日期:2016-08-01 00:00:00
abstract::We previously reported that the dissected pancreatic tissue margin (DPM) and the preoperative serum level of carbohydrate antigen 19-9 (preCA19-9) were independent prognostic factors in pancreatic ductal adenocarcinoma (PDAC). In the current study, the prognostic relevance of these factors, including their molecular a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9839
更新日期:2019-02-01 00:00:00
abstract::Plumbagin, a naphthoquinone constituent of Plumbago zeylanica L. (Plumbaginaceae) is widely used in traditional Chinese medicine as an antifungal, antibacterial and anti-inflammatory agent. Plumbagin is known to exhibit proapoptotic, antiangiogenic and antimetastatic effects in cancer cells. The transcriptional co-fac...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4725
更新日期:2016-08-01 00:00:00
abstract::The present study investigated the anticancer functions of ursolic acid (UA) and its novel derivatives, with a nitrogen-containing heterocyclic scaffold and the privileged fragment at the C-28 position on apoptosis induction, cell proliferation and cell cycle in human BC lines. UA was chemically modified in the presen...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7578
更新日期:2018-02-01 00:00:00
abstract::Pancreatic cancer is one of the most lethal cancers. Transmembrane 4 superfamily member 5 protein (TM4SF5) is one of the candidate molecular targets used for the prevention and treatment of TM4SF5-expressing cancers, including hepatocellular carcinoma, colon cancer and pancreatic cancer. Recently, a previous study rep...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.11134
更新日期:2020-01-01 00:00:00
abstract::The present study aimed to investigate the effects of overexpression of interferon-induced transmembrane protein 5 (IFITM5) and IFITM5 c.-14C>T mutation on osteogenic differentiation, and the proliferation, migration and invasion of SaOS2 cells. SaOS2 cells were transfected with plasmids containing wild type IFITM5 (W...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.5426
更新日期:2017-01-01 00:00:00
abstract::The aim of the present study was to detect the amplification of the human epidermal growth factor receptor 2 (HER2) gene in esophageal squamous cell carcinoma (ESCC), gastroesophageal junction adenocarcinoma (GEJAC) and gastric cancer (GC), as well as to understand the pathological meaning of HER2 gene amplification w...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1348
更新日期:2013-07-01 00:00:00
abstract::The aim of the present study was to investigate whether oridonin is able to increase the effects of lentinan (LNT) in HepG2 human hepatoblastoma cells by MTT, flow cytometry, reverse transcription-quantitative polymerase chain reaction and western blot analysis. The in vitro results demonstrated that 20 µg/ml of orido...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7485
更新日期:2018-02-01 00:00:00
abstract::Cancer stem cells (CSCs) are proposed to be closely correlated with the development and progression of tumors, as well as with chemo- and radioresistance. Targeting CSCs may therefore be a promising potential strategy for the treatment of cancer. Currently, natural products have received great interest due to their th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4431
更新日期:2016-06-01 00:00:00
abstract::Lung cancer is one of the most common malignant cancers in the Czech Republic in men, with the highest mortality rate of all the malignant diseases. The development of biological treatment enables study into novel personalized treatment options. This type of treatment is usually of high quality, and is often demanding...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.7167
更新日期:2017-12-01 00:00:00
abstract::Thyroid cancer is the most common malignant endocrine tumor, with significant morbidity and mortality. Bovine myeloid antimicrobial peptide 28 (BMAP-28) is a cathelicidin that is found in bovine neutrophils. In the present study, the effect and relative mechanism of BMAP-28 on the human thyroid cancer TT cell line in ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3612
更新日期:2015-10-01 00:00:00
abstract::Ilex kudingcha C.J. Tseng (Kudingcha) is a traditional Chinese drink consumed in East Asia. The present study evaluated the in vitro anticancer effects of Kudingcha in MCF-7 human breast adenocarcinoma cells using a 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay. At a concentration of 200 μg/...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1253
更新日期:2013-05-01 00:00:00
abstract::Breast cancer (BC) is the most frequently diagnosed malignant tumor in women worldwide, and the development of new molecules associated with BC is essential for the management of this disease. RAS and EF-hand domain-containing (RASEF) encodes the GTPase enzyme that belongs to the Rab family. Although the effects of th...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9542
更新日期:2018-12-01 00:00:00
abstract::Forkhead box protein A1 (FOXA1) is essential for the growth and differentiation of breast epithelium, and has a favorable outcome in breast cancer (BC). Elevated FOXA1 expression in BC also facilitates hormone responsiveness in estrogen receptor (ESR)-positive BC. However, the interaction between these two pathways is...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6329
更新日期:2017-08-01 00:00:00
abstract::Keloids are a type of benign hyperplasia that cause dermatologic dysfunction and esthetic deformity by invading adjacent normal tissues. Little is known about their etiology, therefore, they are a challenge to treat using plastic surgery. In a previous study, it was demonstrated that the expression of the long non-cod...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8717
更新日期:2018-07-01 00:00:00
abstract::The underlying molecular mechanisms of pancreatic neuroendocrine tumor (pNET) development have not yet been clearly identified. The present study revealed that thrombospondin 2 (THBS2) was downregulated in pNET tissues and cells. Forced expression of THBS2 inhibited the proliferation and migration of pNET cells in vit...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11273
更新日期:2020-03-01 00:00:00