miR-126 suppresses epithelial-to-mesenchymal transition and metastasis by targeting PI3K/AKT/Snail signaling of lung cancer cells.


:Although previous studies have demonstrated that dysregulation of microRNA (miR)-126 is associated with the progression of several types of cancer, including lung cancer, the relationship between miR-126 and lung cancer metastasis remains unclear. SPC-A1 lung cancer cells were transfected with miR-126 mimic and negative control using Lipofectamine® 3000. Following 2 h, TGF-β1 was used to induce epithelial-to-mesenchymal transition (EMT). The protein expression levels of EMT markers: E-cadherin, fibronectin, N-cadherin and vimentin were detected by western blot analysis or immunofluorescence staining. The results demonstrated that ectopic expression of miR-126 significantly suppresses the epithelial-to-mesenchymal transition process, which is considered to be the initial step of tumor metastasis, in SPC-A1 lung cancer cells. In addition, lentivirus-delivered miR-126 was demonstrated to endow Lewis lung carcinoma (LLC) cells with the ability to suppress lung metastasis in vivo. Previous studies have demonstrated that the molecular signals for this phenomenon involve the inhibition of the phosphoinositide 3-kinase/protein kinase B/Snail pathway by miR-126. The protein levels of p-PDK1 (S241) and p-AKT (S473) decreased in miR-126 mimic transfected SPC-A1 and LLC cells, compared with the control group, which were detected by western blot analysis. Reverse transcription-quantitative polymerase chain reaction and western blot analysis results indicated that the expression of Snail decreased in miR-126 mimic transfected SPC-A1 and LLC cells. In conclusion, these results revealed an important role for miR-126 in the regulation of the invasive and metastatic potential of lung cancer, and suggested a potential application for miR-126 in lung cancer treatment.


Oncol Lett


Oncology letters


Jia Z,Zhang Y,Xu Q,Guo W,Guo A




Has Abstract


2018-05-01 00:00:00














  • MicroRNA-203 inhibits cellular proliferation and invasion by targeting Bmi1 in non-small cell lung cancer.

    abstract::MicroRNAs are proposed to serve vital functions in the regulation of tumor progression and invasion. However, the expression levels of miR-203 in non-small cell lung cancer (NSCLC) and its clinical significance remain unknown. In the present study, the association between B-cell-specific moloney murine leukemia virus ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Chen T,Xu C,Chen J,Ding C,Xu Z,Li C,Zhao J

    更新日期:2015-06-01 00:00:00

  • Therapy with transcutaneous administration of imiquimod combined with oral administration of sorafenib suppresses renal cell carcinoma growing in an orthotopic mouse model.

    abstract::Imiquimod is an imidazoquinoline immune response modifier that is used in antiviral and antiallergic creams. Combination therapy using transcutaneous imiquimod and oral sorafenib was previously demonstrated to reduce the tumor burden of renal cell carcinoma growing cutaneously in a mouse model. In the present study, a...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Karashima T,Udaka K,Niimura M,Suzuki K,Osakabe H,Shimamoto T,Fukata S,Inoue K,Kuroda N,Seiki M,Shuin T

    更新日期:2017-07-01 00:00:00

  • Expression and significance of hypoxia-inducible factor-1α and MDR1/P-glycoprotein in laryngeal carcinoma tissue and hypoxic Hep-2 cells.

    abstract::The present study aimed to evaluate the expression of hypoxia-inducible factor-1α (HIF-1α) and MDR1/P-glycoprotein (P-gp) in human laryngeal squamous cell carcinoma (LSCC) tissues, and also to investigate the regulation of MDR1 gene expression by HIF-1α in Hep-2 cells under hypoxic conditions. The expression of HIF-1α...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Xie J,Li DW,Chen XW,Wang F,Dong P

    更新日期:2013-07-01 00:00:00

  • Expression of the tumor suppressor gene hypermethylated in cancer 1 in laryngeal carcinoma.

    abstract::Hypermethylated in cancer 1 (HIC1) is a putative suppressor gene, cooperating with TP53 in the regulation of apoptosis. The promoter site of this gene contains CpG islands susceptible to methylation. Altered methylation leads to the silencing of HIC1. Persistent loss of HIC1 function reflects the attenuation of proapo...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Markowski J,Sieroń AL,Kasperczyk K,Ciupińska-Kajor M,Auguściak-Duma A,Likus W

    更新日期:2015-05-01 00:00:00

  • FISH testing of HER2 immunohistochemistry 1+ invasive breast cancer with unfavorable characteristics.

    abstract::Diagnostic assays for human epidermal growth factor receptor 2 (HER2) expression have a high predictive value because patients with HER2-positive tumors could benefit from HER2-targeted therapy. The aim of the present study is to analyze the incidence of HER2 gene amplification in selected tumors with adverse features...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Petroni S,Caldarola L,Scamarcio R,Giotta F,Latorre A,Mangia A,Simone G

    更新日期:2016-11-01 00:00:00

  • Vitamin D supply: from sun or pill? - Attitudes and recommendation on vitamin D and impact on sun protection practices among German general practitioners evaluated by the network of dermato-oncologists, Onkoderm e.V.

    abstract::Recommendations concerning the intake of vitamin D and/or sunlight exposure in the handling of patients with vitamin D deficiency remain a matter of debate. The present study of the German network of dermato-oncologists (Onkoderm e.V.) refers to an inquiry conducted among general practitioners on this and related issu...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Reinhold U,Dirschka T,Hartgens K,Kirchesch H,Ostendorf R,Petering H,Prieur H,Krähn-Senftleben G,Malaisse WJ

    更新日期:2012-12-01 00:00:00

  • MicroRNA-16 sensitizes drug-resistant breast cancer cells to Adriamycin by targeting Wip1 and Bcl-2.

    abstract::Clinical evidence indicates that drug resistance is a major obstacle in the treatment of breast cancer (BC). Drug resistance results in the disease being uncontrollable, and leads to high mortality rates. The aim of the present study was to investigate the chemosensitizing effect of microRNA (miR)-16 on Adriamycin (AD...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Gao X,Wang M,Zhang Y,Xu Z,Ding J,Tang J

    更新日期:2019-09-01 00:00:00

  • Aspirin inhibits growth of ovarian cancer by upregulating caspase-3 and downregulating bcl-2.

    abstract::[This retracts the article DOI: 10.3892/ol.2016.4607.]. ...

    journal_title:Oncology letters

    pub_type: 杂志文章,撤回出版物


    authors: Li L,Mao X,Qin X,Zhou M,Xing H,Dong F,Jiang X,Zhuang W

    更新日期:2019-05-01 00:00:00

  • Trisomy chromosome 5 is a recurrent cytogenetic lesion in mammary tumors from parous MMTV-erbB-2 transgenic mice.

    abstract::erbB-2 is amplified or overexpressed in approximately 30% of human breast cancers, and has been associated with poor prognosis and therapeutic resistance. Previous studies have suggested that erbB-2 overexpression in transgenic mice induces genomic instability; however, the patterns of genetic lesions vary with indivi...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Kim YM,Ma Z,Lee S,Lee J,Li S,Yang X

    更新日期:2011-11-01 00:00:00

  • Desmoid-type fibromatosis of the breast: A report of 2 cases.

    abstract::Mammary fibromatosis is a rare and locally aggressive benign tumor of the breast; it originates from fibroblasts and myofibroblasts within the breast parenchyma and does not metastasize. The condition is locally aggressive and has a high rate of recurrence. The etiology of mammary fibromatosis is unknown. Breast imagi...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Benej R,Mečiarová I,Pohlodek K

    更新日期:2017-08-01 00:00:00

  • Carbohydrate antigen 19-9 as a prognostic biomarker in pancreatic neuroendocrine tumors.

    abstract::Carbohydrate antigen 19-9 (CA19-9) is not generally considered to be a biomarker in pancreatic neuroendocrine tumors (pNETs), as the majority of pNETs present with a normal range of CA19-9. The present study aimed to evaluate the role of serum CA19-9 levels as a prognostic factor in a relatively large number of patien...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Luo G,Jin K,Cheng H,Liu C,Guo M,Lu Y,Yang C,Xu J,Wang W,Gao H,Zhang S,Long J,Xu J,Ni Q,Chen J,Yu X

    更新日期:2017-12-01 00:00:00

  • Overexpression of long noncoding RNA PEG10 promotes proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma.

    abstract::The present study aimed to investigate the impact of overexpression of long noncoding RNA PEG10 (lncRNA PEG10) on the proliferation, invasion and metastasis of hypopharyngeal squamous cell carcinoma (HSCC). Quantitative reverse transcription polymerase chain reaction was used to quantify lncRNA PEG10 expression levels...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhao M,Sun D,Li X,Xu Y,Zhang H,Qin Y,Xia M

    更新日期:2017-09-01 00:00:00

  • Downregulation of TACC3 inhibits tumor growth and migration in osteosarcoma cells through regulation of the NF-κB signaling pathway.

    abstract::TACC3, a member of the transforming acidic coiled-coil protein (TACC) family, is a multifunctional protein that is involved in various biological functions, including proliferation and differentiation of tumor cells, cancer progression and metastasis. The aims of the present study were to examine whether TACC3 express...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhao C,He X,Li H,Zhou J,Han X,Wang D,Tian G,Sui F

    更新日期:2018-05-01 00:00:00

  • Fibrosarcomatous pigmented dermatofibrosarcoma protuberans: A case report with review of the literature.

    abstract::Dermatofibrosarcoma protuberans (DFSP) is a relatively rare low-grade sarcoma that occasionally exhibits melanin-containing spindle cells within the tumor. Fibrosarcomatous DFSP (FS-DFSP) is a variant that is characterized by areas which are histopathologically indistinguishable from fibrosarcoma. In the present study...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Ishida M,Okabe H

    更新日期:2012-09-01 00:00:00

  • Targeted sequencing reveals distinct pathogenic variants in Chinese patients with lung adenocarcinoma brain metastases.

    abstract::Lung cancer is the most common type of malignancy to metastasize to the brain, with the median survival time of patients being 6-11 months. In the present study, the aim was to compare the actionable gene mutation profiles of primary lung adenocarcinoma (LC) samples and LC brain metastasis (LCBM) samples through targe...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Ma Y,Chen K,Yang Z,Guan M

    更新日期:2018-04-01 00:00:00

  • Piper betle extracts exhibit antitumor activity by augmenting antioxidant potential.

    abstract::The present study was conducted to evaluate the methanolic extract of Piper betle leaves (MPBL) and its organic fractions with regard to antitumor activity against Ehrlich ascites carcinoma (EAC) in Swiss albino mice and to confirm their antioxidant activities. At 24 h post-intraperitoneal inoculation of tumor cells i...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Alam B,Majumder R,Akter S,Lee SH

    更新日期:2015-02-01 00:00:00

  • DNA hypomethylation of interleukin 8 in clear cell renal cell carcinoma.

    abstract::DNA hypomethylation is associated with carcinogenesis due to its involvement in cancer initiation and progression. In this study we analyzed the hypomethylation status of candidate genes in clear cell renal cell carcinoma (CCRCC) using a large-scale, high-throughput DNA methylation profiling technique. Results reveale...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Yoo KH,Park YK,Chang SG

    更新日期:2013-01-01 00:00:00

  • Methylation of CDKN2B CpG islands is associated with upregulated telomerase activity in children with acute lymphoblastic leukemia.

    abstract::The aim of the present study was to investigate the association between methylation of cyclin-dependent kinase inhibitor 2B (CDKN2B) CpG islands and telomerase activity in children with acute lymphoblastic leukemia (ALL). A total of 72 children with ALL and 12 children with immune thrombocytopenia (ITP) were subjected...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Hu Q,Chen X,Liu S,Wen R,Yuan X,Xu D,Liu G,Wen F

    更新日期:2017-04-01 00:00:00

  • miR-451a suppression of IL-6R can inhibit proliferation and increase apoptosis through the JAK2/STAT3 pathway in multiple myeloma.

    abstract::The IL-6R/JAK2/STAT3 pathway mediated by interleukin-6 (IL-6) plays an important role in the occurrence and development of multiple myeloma (MM), which is associated with decreased microRNA-451a. However, the biological function of microRNA-451a in MM remains unclear. The bone marrow (BM) of patients with MM was sampl...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhong L,Xu Z,Jin X,He Y,Zhang J,Jiang T,Chen J

    更新日期:2020-12-01 00:00:00

  • Overexpression of RHEB is associated with metastasis and poor prognosis in hepatocellular carcinoma.

    abstract::Aberrant expression of Ras homolog enriched in brain (RHEB) has been observed in a variety of cancer tissues and is closely associated with clinicopathological features. However, the expression profile of RHEB in patients with hepatocellular carcinoma (HCC) and its clinical signature with underlying mechanisms have no...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Liu F,Pan Z,Zhang J,Ni J,Wang C,Wang Z,Gu F,Dong W,Zhou W,Liu H

    更新日期:2018-03-01 00:00:00

  • A polysaccharide isolated from Agaricus blazei Murill (ABP-AW1) as a potential Th1 immunity-stimulating adjuvant.

    abstract::In the present study, a low molecular weight polysaccharide, ABP-AW1, isolated from Agaricus blazei Murill was assessed for its potential adjuvant activity. ABP-AW1 is considered to create a 'depot' of antigen at a subcutaneous injection site. ICR mice were immunized with 100 μg ovalbumin (OVA) alone or with 100 μg OV...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Cui L,Sun Y,Xu H,Xu H,Cong H,Liu J

    更新日期:2013-10-01 00:00:00

  • Clinical value of magnetic resonance imaging in preoperative T staging of gastric cancer and postoperative pathological diagnosis.

    abstract::The aim of the present study was to evaluate the clinical value of magnetic resonance imaging (MRI) in the preoperative T staging of gastric cancer and in the postoperative pathological diagnosis. In total, 30 patients with gastric cancer were investigated, including 19 males and 11 females (age, 50-69 years; mean age...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Huo X,Yuan K,Shen Y,Li M,Wang Q,Xing L,Shi G

    更新日期:2014-07-01 00:00:00

  • Linc-OIP5 loss regulates migration and invasion in MDA-MB-231 breast cancer cells by inhibiting YAP1/JAG1 signaling.

    abstract::Breast cancer is the most prevalent cancer among women, and diagnosis and treatment represent a substantial challenge due to the lack of adequate molecular targets. It has been shown that long noncoding RNAs (lncRNAs) serve pivotal roles in regulating gene expression in tumors. The roles of long intervening noncoding ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Zhu Q,Li Y,Dong X,Yang Y,Wang H,Guo S

    更新日期:2020-01-01 00:00:00

  • Expression and regulatory effects of microRNA-182 in osteosarcoma cells: A pilot study.

    abstract::The aim of the present study was to evaluate the expression level of microRNA-182 (miRNA-182) in human osteosarcoma (OS) MG-63 cells and OS tissues, and to elucidate the effect of miRNA-182 on the biological activity of tumors. In the present study, the expression of miRNA-182 in human OS MG-63 cells, OS tissues and n...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Bian DL,Wang XM,Huang K,Zhai QX,Yu GB,Wu CH

    更新日期:2016-05-01 00:00:00

  • Activating BRAF gene mutations are uncommon in hormone refractory prostate cancer in Caucasian patients.

    abstract::Activating mutations in the cytosolic serine/threonine kinase, BRAF, have been reported in a variety of neoplasms. BRAF activation may contribute to tumor growth via activation of the MAP/ERK kinase pathway, and BRAF represents a possible therapeutic target. Activating BRAF mutations were recently reported in approxim...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Köllermann J,Albrecht H,Schlomm T,Huland H,Graefen M,Bokemeyer C,Simon R,Sauter G,Wilczak W

    更新日期:2010-07-01 00:00:00

  • Leucine zipper, down regulated in cancer-1 gene expression in prostate cancer.

    abstract::Numerous genetic alterations have been implicated in the development of prostate cancer (PCa). DNA and protein microarrays have enabled the identification of genes associated with apoptosis, which is important in PCa development. Despite the molecular mechanisms are not entirely understood, inhibition of apoptosis is ...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Salemi M,Barone N,La Vignera S,Condorelli RA,Recupero D,Galia A,Fraggetta F,Aiello AM,Pepe P,Castiglione R,Vicari E,Calogero AE

    更新日期:2016-10-01 00:00:00

  • A report of amelanotic malignant melanoma of the esophagus diagnosed appropriately with novel markers: A case report.

    abstract::The present case study reported of amelanotic malignant melanoma of the esophagus. A 68-year-old man underwent laparoscopic distal gastrectomy for early gastric cancer diagnosis. After gastrectomy, endoscopic examination revealed a protruded lesion lying adjacent to the melanosis area of the esophagus. Histology of th...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Kobayashi J,Fujimoto D,Murakami M,Hirono Y,Goi T

    更新日期:2018-06-01 00:00:00

  • Short hairpin RNA of frizzled-2 suppresses the proliferation of hepatocellular carcinoma cells.

    abstract::In the present study, Frizzled-2 (Fz2), a receptor of the Wnt ligand, was investigated as a potential target of molecular therapy for hepatocellular carcinoma (HCC). Quantitative polymerase chain reaction (PCR) was performed to determine the expression levels of Fz2. A surgical specimen of HCC was immunostained with a...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Tomizawa M,Shinozaki F,Motoyoshi Y,Sugiyama T,Yamamoto S,Sueishi M

    更新日期:2014-10-01 00:00:00

  • Secretagogin, a marker for neuroendocrine cells, is more sensitive and specific in large cell neuroendocrine carcinoma compared with the markers CD56, CgA, Syn and Napsin A.

    abstract::A common method to distinguish large cell neuroendocrine carcinoma (LCNEC) from non-neuroendocrine large cell carcinoma (non-NE LCC) is from using specific immunohistochemistry markers, such as CgA, Syn, CD56 and Napsin A, however, the results remain controversial using these markers. Secretagogin (SCGN) is a newly di...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Dong Y,Li Y,Liu R,Li Y,Zhang H,Liu H,Chen J

    更新日期:2020-03-01 00:00:00

  • Multiple regulatory mechanisms of hepatocyte growth factor expression in malignant cells with a short poly(dA) sequence in the HGF gene promoter.

    abstract::Hepatocyte growth factor (HGF) expression is a poor prognostic factor in various types of cancer. Expression levels of HGF have been reported to be regulated by shorter poly(dA) sequences in the promoter region. In the present study, the poly(dA) mononucleotide tract in various types of human cancer cell lines was exa...

    journal_title:Oncology letters

    pub_type: 杂志文章


    authors: Sakai K,Takeda M,Okamoto I,Nakagawa K,Nishio K

    更新日期:2015-01-01 00:00:00