Abstract:
:The aim of the present study was to investigate whether oridonin is able to increase the effects of lentinan (LNT) in HepG2 human hepatoblastoma cells by MTT, flow cytometry, reverse transcription-quantitative polymerase chain reaction and western blot analysis. The in vitro results demonstrated that 20 µg/ml of oridonin was a nontoxic concentration for L02 normal liver cells and HepG2 liver cancer cells. Furthermore, treatment with 0-200 µg/ml LNT was only able to decrease the viability of HepG2 liver cancer cells. The growth inhibitory rate of the LNT-L (100 µg/ml) treatment group was 20.7% and the rate of the LNT-H (200 µg/ml) treatment group was 54.8%. Notably, the growth inhibitory rate of the oridonin + LNT-H group was 84.3%. The highest percentage of apoptotic cells was observed in the oridonin + LNT-H group (20 µg/ml oridonin and 200 µg/ml LNT). The percentage of apoptotic cells in the oridonin + LNT-H group was significantly different from the percentage of apoptotic cells in the LNT-H (26.1%) and the LNT-L (16.8%) groups. Treatment with LNT produced an increase in caspase-3, caspase-9, Bcl-2-like protein 4, p53, p21, nuclear factor κB inhibitor-α mRNA and protein expression and a decrease in B-cell lymphoma 2 and nuclear factor-κB expression in HepG2 cells compared with untreated control cells. Treatment with a combination of oridonin and LNT-H induced a further increase in expression with the biggest differences in expression observed between the oridonin + LNT-H group and control. It was observed that treatment with oridonin was able to increase the anticancer effects of LNT in HepG2 cells. Therefore, oridonin may be used to sensitize cells to LNT.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Sun Z,Han Q,Duan L,Yuan Q,Wang Hdoi
10.3892/ol.2017.7485subject
Has Abstractpub_date
2018-02-01 00:00:00pages
1999-2005issue
2eissn
1792-1074issn
1792-1082pii
OL-0-0-7485journal_volume
15pub_type
杂志文章相关文献
Oncology Letters文献大全abstract::Synovial sarcoma (SS) is a soft tissue sarcoma of unknown histogenesis that rarely occurs in the female genital tract. We report a case of SS occurring in the right vulva of a young Japanese female. The tumor was composed of poorly differentiated rounded cell areas, surrounded by fibroblastic spindle-shaped cell areas...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2012.857
更新日期:2012-11-01 00:00:00
abstract::The present study aimed to identify differences in protein expression in cases of endometrioid endometrial cancer (EEC) with and without coexisting adenomyosis uteri (AM), and to evaluate the histopathological and prognostic distinctions. The total cohort included 22 patients in Group A (patients with concomitant AM a...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6592
更新日期:2017-09-01 00:00:00
abstract::Application of dexmedetomidine combined with sufentanil in colon cancer resection and its effect on immune and coagulation function of patients was studied. Colon cancer cases (n=176) admitted to Xiangya Hospital Central South University were selected into the study. They were divided into group A (n=92) and group B (...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.11643
更新日期:2020-08-01 00:00:00
abstract::Mangiferin, a flavonoid extracted from the mango tree, possesses anti-inflammatory, antibacterial, anti-herpes simplex and antitumor activity, and is able to affect immune function. The present study investigated the anticancer effects of mangiferin treatment on PC3 human prostate cancer cells, and the potential under...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3924
更新日期:2016-01-01 00:00:00
abstract::Nervous system polycomb 1 (NSPc1) is a member of the polycomb group (PcG) family of proteins and has been demonstrated to maintain the differentiation and pluripotency of stem cells. Long non-coding RNAs (lncRNAs) have been demonstrated to be involved in the control of pluripotency and differentiation in embryonic and...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10254
更新日期:2019-06-01 00:00:00
abstract::Mammary fibromatosis is a rare and locally aggressive benign tumor of the breast; it originates from fibroblasts and myofibroblasts within the breast parenchyma and does not metastasize. The condition is locally aggressive and has a high rate of recurrence. The etiology of mammary fibromatosis is unknown. Breast imagi...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6337
更新日期:2017-08-01 00:00:00
abstract::Hepatocellular carcinoma (HCC) is one of the leading digestive malignancies, with a high metastasis and recurrence. The development and rapid progression of HCC involves numerous complex molecular and cellular events. Therefore, developing effective methods for the prevention and treatment of HCC requires an improved ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6369
更新日期:2017-08-01 00:00:00
abstract::The correlation between the expression levels of p65 and TNF-α in patients with acute myelocytic leukemia (AML) and AML cell lines were investigated. The bone marrow samples of 30 AML patients and 10 non-leukemia controls were studied. The mRNA expression levels of p65 and TNF-α were detected by reverse transcription-...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3720
更新日期:2015-11-01 00:00:00
abstract::Significant antitumor activity of Momordica anti-human immunodeficiency virus protein of 30 kDa (MAP30) purified from Momordica charantia has been the subject of previous research. However, the effective mechanism of MAP30 on malignant glioma cells has not yet been clarified. The aim of the present study was to invest...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8073
更新日期:2018-04-01 00:00:00
abstract::Poly(ADP-Ribose) polymerase family member 9 (PARP9) promotes the proliferation, survival and chemotherapy resistance in lymphoma and prostate cancer. The expression and function of PARP9 in human breast cancer remains unknown. In the present study, it was demonstrated that PARP9 is frequently overexpressed in human br...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9124
更新日期:2018-09-01 00:00:00
abstract::Hepatocellular carcinoma is a serious public health problem in China. The mortality rate associated with the majority of cancer types has decreased as a result of targeted therapy. However, the mortality rates associated with hepatocellular carcinoma have not improved; therefore, the identification of new molecular ta...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12245
更新日期:2020-12-01 00:00:00
abstract::The aim of this study was to investigate the characteristics of serum secreted frizzled-related protein 5 (SFRP5), an inhibitor of Wnt signaling, in hepatitis B virus (HBV)-associated infections and hepatocellular carcinoma (HCC) patients. Serum SFRP5 levels were detected in 147 patients with HBV-associated chronic in...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2256
更新日期:2014-09-01 00:00:00
abstract::The present study examined the expression of podocalyxin (PODX) in surgically-resected astrocytomas, associated the levels of PODX expression with the clinicopathological characteristics and survival outcomes of astrocytoma and assessed how PODX affected the viability of astrocytoma cells following the administration ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2013.1556
更新日期:2013-11-01 00:00:00
abstract::The aim of the present study was to investigate the effects of the heat shock protein (HSP)90 inhibitor AUY922 on the proliferation and migratory ability of renal cell carcinoma (RCC) cells. The expression of HSP90 was measured in vitro using western blotting and quantitative polymerase chain reaction in the ACHN and ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3299
更新日期:2015-08-01 00:00:00
abstract::Although bulk high-throughput genomic profiling studies have led to a significant increase in the understanding of cancer biology, there is increasing awareness that bulk profiling approaches do not completely elucidate tumor heterogeneity. Single-cell genomic profiling enables the distinction of tumor heterogeneity, ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.5669
更新日期:2017-03-01 00:00:00
abstract::Chemotherapy failure remains a challenge when treating patients with acute myeloid leukemia (AML), who often suffer from persistent or relapsed disease. The multidrug resistance (MDR) mediated by efflux transporters of the ATP binding cassette (ABC) superfamily is a major obstacle for successful chemotherapy. The pres...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8095
更新日期:2018-05-01 00:00:00
abstract::Renal oncocytoma is one of the most unusual benign lesion, which are usually diagnosed postoperatively, since their differentiation from renal cell carcinoma is challenging. The present study reports two cases of renal oncocytoma in a 60-year-old woman and a 46-year-old man. Relevant clinical and pathological data on ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4594
更新日期:2016-07-01 00:00:00
abstract::Lung cancer is a major cause of death worldwide, and non-small cell lung cancer (NSCLC) is the most common type of lung cancer. The aim of this study was to investigate whether miR-96 mediated the invasion and metastasis of NSCLC by targeting glypican-3 (GPC3). Reverse transcription-quantitative PCR (RT-qPCR) was empl...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8507
更新日期:2018-06-01 00:00:00
abstract::Glycocholic acid (GCA) is a novel identified biomarker for hepatocellular carcinoma (HCC). However, clinical pathological study of GCA has not been extensive due to the limited availability of anti-GCA monoclonal antibodies (mAbs) and restricted detection methods. In the present study, using human GCA conjugated with ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.9943
更新日期:2019-03-01 00:00:00
abstract::Research on the immunosuppression of cancer cells has attracted much attention in recent years. The present study sought to provide a new strategy for tumor immunotherapy targeting mast cells by studying the mechanisms underlying mast cell function in cancer immunosuppression. Between January 2015 and December 2017, t...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2020.12224
更新日期:2020-12-01 00:00:00
abstract::The aim of the present study was to investigate the ability of Nimotuzumab to increase radiosensitivity at different delivery times in the mixed cancer cell line Eca109, to determine the optimal delivery time. Cultured Eca109 cells were classified into five groups: Control with no treatment (O group); irradiation with...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.9897
更新日期:2019-03-01 00:00:00
abstract::The aim of the present study was to identify the differentially expressed microRNAs (DEMs) between Lynch syndrome (LS) and the normal colonic (N-C) control samples, predict the target genes (TGs) and analyze the potential functions of the DEMs and TGs. The miRNA expression dataset GSE30454, which included data of 13 L...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2016.4816
更新日期:2016-09-01 00:00:00
abstract::Ovarian cancer is the most malignant gynecologic neoplasm in women and has the worst prognosis of all cancer types in women based on the 5-year survival rates. A previous study indicated that mangiferin exerts an anti-neoplastic effect on human ovarian cancer cells by targeting Notch3. Additionally, it has been demons...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9708
更新日期:2019-01-01 00:00:00
abstract::Bladder cancer (BC) is one of the most common urogenital malignancies. However, present studies of its multiple gene interaction and cellular pathways remain unable to accurately verify the genesis and the development of BC. The aim of the present study was to investigate the genetic signatures of BC and identify its ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.9002
更新日期:2018-09-01 00:00:00
abstract::The endogenous lipid metabolism network is associated with the occurrence and progression of malignancies. Endocannabinoids and ceramides have demonstrated their anti-proliferative and pro-apoptotic properties in a series of cancer studies. The aim of the present study was to evaluate the expression patterns of endoca...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10399
更新日期:2019-08-01 00:00:00
abstract::Gastric cancer (GC) is the second leading cause of cancer-related mortality worldwide. The usual treatment of GC consists of surgery with additional adjuvant chemotherapy. In the present study, the feasibility and safety of adjuvant S-1 plus oxaliplatin (SOX) chemotherapy for patients with GC and the optimal dosage of...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2014.2821
更新日期:2015-03-01 00:00:00
abstract::Urothelial cancer is the most frequently diagnosed type of malignant tumor in the bladder, of which primary adenocarcinoma accounts for a small percentage. Secondary malignancies, in particular metastatic adenocarcinoma from the lung, are exceedingly rare, with only six cases previously reported in the literature. The...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2015.3445
更新日期:2015-09-01 00:00:00
abstract::In patients with diffuse large B-cell lymphoma (DLBCL) classified as high-intermediate risk or high risk using the International Prognosis Index, the efficacy of high-dose chemotherapy combined with upfront autologous stem cell transplantation (HDT/ASCT) remains controversial in the rituximab era. In the present study...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2017.6589
更新日期:2017-09-01 00:00:00
abstract::Expression level of miR-181c in neuroblastoma children and its effect on proliferation of neuroblastoma M17 cells were investigated. Fifty-seven neuroblastoma patients admitted to Weifang People's Hospital from January 2013 to December 2017 were selected and their cancer tissues and normal adjacent tissues were obtain...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2019.10602
更新日期:2019-09-01 00:00:00
abstract::Hepcidin and ferroportin, which are known as key iron regulators, may be used in future treatments of pancreatic ductal adenocarcinoma. Iron is essential for life support; it helps oxygen molecules bind to hemoglobin and acts as an important catalytic enzyme center. However, iron overload is a risk factor for cancer, ...
journal_title:Oncology letters
pub_type: 杂志文章
doi:10.3892/ol.2018.8357
更新日期:2018-05-01 00:00:00