MAP30 promotes apoptosis of U251 and U87 cells by suppressing the LGR5 and Wnt/β-catenin signaling pathway, and enhancing Smac expression.

Abstract:

:Significant antitumor activity of Momordica anti-human immunodeficiency virus protein of 30 kDa (MAP30) purified from Momordica charantia has been the subject of previous research. However, the effective mechanism of MAP30 on malignant glioma cells has not yet been clarified. The aim of the present study was to investigate the effects and mechanism of MAP30 on U87 and U251 cell lines. A Cell Counting Kit-8 assay, wound healing assay and Transwell assay were used to detect the effects on U87 and U251 cells treated with different concentrations of MAP30 (0.5, 1, 2, 4, 8 and 16 µM) over different periods of time. Proliferation, migration and invasion of each cell line were markedly inhibited by MAP30 in a dose- and time-dependent manner. Flow cytometry and fluorescence staining demonstrated that apoptosis increased and the cell cycle was arrested in S-phase in the two investigated cell lines following MAP30 treatment. Western blot analysis demonstrated that leucine-rich-repeat-containing G-protein-coupled receptor 5 (LGR5) expression and key proteins in the Wnt/β-catenin signaling pathway were apparently decreased, whereas second mitochondria-derived activator of caspase (Smac) protein expression significantly increased with MAP30 treatment in the same manner. These results suggest that MAP30 markedly induces apoptosis in U87 and U251 cell lines by suppressing LGR5 and the Wnt/β-catenin signaling pathway, and enhancing Smac expression in a dose- and time-dependent manner.

journal_name

Oncol Lett

journal_title

Oncology letters

authors

Jiang Y,Miao J,Wang D,Zhou J,Liu B,Jiao F,Liang J,Wang Y,Fan C,Zhang Q

doi

10.3892/ol.2018.8073

subject

Has Abstract

pub_date

2018-04-01 00:00:00

pages

5833-5840

issue

4

eissn

1792-1074

issn

1792-1082

pii

OL-0-0-8073

journal_volume

15

pub_type

杂志文章
  • Introduction and utility of liquid-based cytology on aspiration biopsy of peripheral nodular lesions of the lung.

    abstract::In the present study, aspiration biopsy cytology (ABC) was used for the diagnosis of peripheral nodular lesions in the lung (PNLL), and liquid-based cytology (LBC) was carried out on the material collected to evaluate it in comparison with the conventional method (CM). The subjects comprised 130 cases that underwent c...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1763

    authors: Imura J,Abe K,Uchida Y,Shibata M,Tsunematsu K,Sathoh M,Miwa S,Nakajima T,Nomoto K,Hayashi S,Tsuneyama K

    更新日期:2014-03-01 00:00:00

  • Zerumbone inhibits melanoma cell proliferation and migration by altering mitochondrial functions.

    abstract::It has been reported that zerumbone (ZER) has marked effects on the regulation of cell proliferation and migration in multiple types of cancer, and has anti-cancer effects on various types of malignant cell. However, the effects and underlying molecular mechanisms of treatment with ZER on melanoma cells remain unclear...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.5742

    authors: Yan H,Ren MY,Wang ZX,Feng SJ,Li S,Cheng Y,Hu CX,Gao SQ,Zhang GQ

    更新日期:2017-04-01 00:00:00

  • Genetic alterations in epidermal growth factor receptor-tyrosine kinase inhibitor-naïve non-small cell lung carcinoma.

    abstract::Epidermal growth factor receptor (EGFR)-tyrosine kinase inhibitors (TKIs) are an approved first-line therapy against unresectable or advanced non-small cell lung cancer (NSCLC) harboring EGFR gene activating mutations. However, the majority of tumors develop acquired resistance against EGFR-TKIs and some tumors exhibi...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11524

    authors: Yamaura T,Muto S,Mine H,Takagi H,Watanabe M,Ozaki Y,Inoue T,Fukuhara M,Okabe N,Matsumura Y,Hasegawa T,Osugi J,Hoshino M,Higuchi M,Shio Y,Suzuki H

    更新日期:2020-06-01 00:00:00

  • A novel POLH mutation causes XP-V disease and XP-V tumor proneness may involve imbalance of numerous DNA polymerases.

    abstract::Xeroderma pigmentosum variant (XP-V) is a subtype of xeroderma pigmentosum (XP) disease with typical pigmentation and types of cancer in the oral maxillofacial and other sun-exposed regions. Few factors of tumor proneness in XP-V have been completely elucidated with the exception of the POLH [which encodes DNA polymer...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1604

    authors: Guo J,Zhou G,Zhang W,Song Y,Bian Z

    更新日期:2013-12-01 00:00:00

  • Sigmoid adenocarcinoma with metastases to the kidney: Report of a rare case and review of the literature.

    abstract::Distant metastases from colorectal cancer (CRC) most frequently spread to the liver and lungs. CRC metastasis to the kidney is extremely rare and may be generally associated with an unfavorable prognosis. Thus, patients with metastatic CRC and kidney metastasis are a diagnostic and therapeutic challenge. The present s...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2015.3290

    authors: Dulskas A,Bagurskas P,Sinkevicius Z,Samalavicius NE

    更新日期:2015-08-01 00:00:00

  • Hsa-miR-599 suppresses the migration and invasion by targeting BRD4 in breast cancer.

    abstract::Breast cancer is the second leading cause of cancer-associated mortality in females in the USA. Hsa-miR-599 was demonstrated to function as a tumour suppressor during cancer progression. However, the function and mechanism of the hsa-miR-599 in human breast cancer remain elusive. Thus, the aim of the present study was...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.6651

    authors: Wang Y,Sui Y,Zhu Q,Sui X

    更新日期:2017-09-01 00:00:00

  • Actein inhibits cell proliferation and migration and promotes cell apoptosis in human non-small cell lung cancer cells.

    abstract::Non-small cell lung cancer (NSCLC) is the leading cause of death in smokers and the most common cause for cancer mortality in both males and females in the United States. Predisposition of this malignancy to distant metastasis leads to poor prognosis; therefore, it is urgent to discover novel therapeutic agents for me...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.7668

    authors: Zhang Y,Lian J,Wang X

    更新日期:2018-03-01 00:00:00

  • Effect of synchronous solitary bone metastasectomy and lung cancer resection on non-small cell lung cancer patients.

    abstract::Lung cancer is the most frequent cause of cancer-associated mortality among men and women globally. The skeleton is one of the most common metastatic sites. The majority of patients exhibiting bone metastases are treated using systemic therapy or symptom-based palliative approaches without surgery. The present study a...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.4190

    authors: Zhao T,Gao Z,Wu W,He W,Yang YI

    更新日期:2016-03-01 00:00:00

  • Ubiquitin-like modifier-activating enzyme 7 as a marker for the diagnosis and prognosis of breast cancer.

    abstract::Ubiquitin-like modifier-activating enzyme 7 (UBA7) is a specific E1-like ubiquitin-activating enzyme involved in interferon-stimulated gene 15 (ISG15) conjugation. UBA7 expression has been reported to be notably decreased in lung cancer. The present study aimed to investigate the changes in UBA7 expression in breast c...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11406

    authors: Lin M,Li Y,Qin S,Jiao Y,Hua F

    更新日期:2020-04-01 00:00:00

  • Sometimes it is better to wait: First Italian case of a newborn with transient abnormal myelopoiesis and a favorable prognosis.

    abstract::Congenital leukemia is rare disease with an incidence of one to five cases per million births. Transient abnormal myelopoiesis (TAM), also called transient myeloproliferative disorder, is a pre-leukemia disorder that may occur in Down syndrome (DS) or non-DS infants. TAM may enter spontaneous remission; however, conti...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2016.5401

    authors: Salvatori G,Foligno S,Sirleto P,Genovese S,Russo S,Coletti V,Dotta A,Luciani M

    更新日期:2017-01-01 00:00:00

  • Decreased copy number of mitochondrial DNA: A potential diagnostic criterion for gastric cancer.

    abstract::An alteration in the mitochondrial DNA (mtDNA) copy number has been detected in numerous human cancers. However, certain changes in the mtDNA copy number that occur during the initiation and progression of gastric cancer remain undetected. In the present study, using quantitative PCR analysis, the quantitative changes...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1492

    authors: Wen SL,Zhang F,Feng S

    更新日期:2013-10-01 00:00:00

  • Comparison of laryngeal mask airway and endotracheal intubation in gynecological cancer operation.

    abstract::Endotracheal intubation (ETI) and laryngeal mask airway (LMA) in terms of hemodynamics and reaction were compared. A total of 54 general anesthesia patients were randomized into two groups with 27 cases in each group. Acceleration index (ACI), cardiac index (CI), cardiac output (CO), left cardiac work (LCW), systemic ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2018.9813

    authors: Song Z,Tan J,Fang J,Bian Q,Gu L

    更新日期:2019-02-01 00:00:00

  • Intranasal dexmedetomidine combined with local anesthesia for conscious sedation during breast lumpectomy: A prospective randomized trial.

    abstract::Breast lumpectomy is usually performed under general or local anesthesia. To the best of our knowledge, whether conscious sedation with intranasal dexmedetomidine and local anesthesia is an effective anesthetic technique has not been studied. Thus, the present study aimed to investigate the effectiveness of conscious ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11938

    authors: Yuan YJ,Zhou P,Xia F,Zhang XB,He SS,Guo DY,Xing YH,Zhao HW

    更新日期:2020-10-01 00:00:00

  • The modulation study of multiple drug resistance in bladder cancer by curcumin and resveratrol.

    abstract::Gemcitabine (GCB), which functions via the inhibition of DNA synthesis, is commonly used in the treatment of bladder cancer; however, its response rate is not satisfactory due to the development of drug resistance. The potential for phytochemicals to reverse drug resistance in bladder cancer tumor cells was evaluated....

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.11023

    authors: Cho CJ,Yang CW,Wu CL,Ho JY,Yu CP,Wu ST,Yu DS

    更新日期:2019-12-01 00:00:00

  • Resistance to nanoparticle albumin-bound paclitaxel is mediated by ABCB1 in urothelial cancer cells.

    abstract::Nanoparticle albumin-bound (nab)-paclitaxel appears to exhibit better response rates in patients with metastatic urothelial cancer of the bladder whom are pretreated with nab-paclitaxel compared with conventional paclitaxel. Paclitaxel may induce multidrug resistance in patients with cancer, while the mechanisms of re...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.5986

    authors: Vallo S,Köpp R,Michaelis M,Rothweiler F,Bartsch G,Brandt MP,Gust KM,Wezel F,Blaheta RA,Haferkamp A,Cinatl J Jr

    更新日期:2017-06-01 00:00:00

  • Mecambridine induces potent cytotoxic effects, autophagic cell death and modulation of the mTOR/PI3K/Akt signaling pathway in HSC-3 oral squamous cell carcinoma cells.

    abstract::Plant secondary metabolites including alkaloids, demonstrate a complex diversity in their molecular scaffolds and exhibit tremendous pharmacological potential as anti-cancerous therapeutics. The present study aimed to evaluate the anticancer activity of a natural alkaloid, mecambridine, against human oral squamous cel...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.7321

    authors: Lin N,Li Z,Wang D,Zheng K,Wu Y,Wang H

    更新日期:2018-01-01 00:00:00

  • Relationship between DLK1 gene promoter region DNA methylation and non-small cell lung cancer biological behavior.

    abstract::We investigated the possible association between DLKI gene promoter region methylation and the increased invasion capacity of non-small cell lung cancer (NSCLC). Lung cancer cell line H1299, as well as the gene transfection and RNA interference technology were used to build DLK gene overexpression and knockdown cells....

    journal_title:Oncology letters

    pub_type: 杂志文章,收录出版

    doi:10.3892/ol.2017.6019

    authors: Zhong Z,Ye Y,Guo W,He Y,Hu W

    更新日期:2017-06-01 00:00:00

  • Transcript levels of ten-eleven translocation type 1-3 in cervical cancer and non-cancerous cervical tissues.

    abstract::Decreased expression of ten-eleven translocation (TET1, TET2 and TET3) proteins has been reported in various types of cancer. However, the expression levels of TET proteins in cervical cancer (CC) remain to be elucidated. The present study determined the levels of TET1, TET2 and TET3 transcripts in cancerous (n=80) an...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2017.5930

    authors: Bronowicka-Kłys DE,Roszak A,Pawlik P,Sajdak S,Sowińska A,Jagodziński PP

    更新日期:2017-05-01 00:00:00

  • miR-223 functions as a potent tumor suppressor of the Lewis lung carcinoma cell line by targeting insulin-like growth factor-1 receptor and cyclin-dependent kinase 2.

    abstract::microRNAs (miRNAs) have been hypothesized to function as oncogenes or tumor suppressors by targeting specific cancer-related genes. Previous studies have reported that miR-223 may serve as a tumor suppressor in a number of cancer types, however, knowledge of its targets in non-small cell lung cancer (NSCLC) remains li...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1375

    authors: Nian W,Ao X,Wu Y,Huang Y,Shao J,Wang Y,Chen Z,Chen F,Wang D

    更新日期:2013-08-01 00:00:00

  • Downregulation of ST3GAL5 is associated with muscle invasion, high grade and a poor prognosis in patients with bladder cancer.

    abstract::In patients with bladder cancer (BC), the association between ST3 β-galactoside α-2,3-sialyltransferase 5 (ST3GAL5) expression and clinical outcomes, particularly regarding muscle-invasive disease, high tumor grade and prognosis, remain unknown. In the present study, the expression of ST3GAL5 and its association with ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11597

    authors: Ouyang S,Liu JH,Ni Z,Ding GF,Wang QZ

    更新日期:2020-07-01 00:00:00

  • Aggressive osteoblastoma of the sphenoid bone.

    abstract::For osteoblastoma, with its predilection for the spinal column and appendicular skeleton, the skull is an unusual site, and paranasal sinus involvement is very rare. Herein, we report on a case in which the disease was located within the sphenoid bone. To the best of our knowledge, this is the 4th reported case of ost...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol_00000065

    authors: Kukwa W,Oziębło A,Oecińska A,Czarnecka AM,Włodarski K,Kukwa A

    更新日期:2010-03-01 00:00:00

  • Anesthetic effect of propofol combined with remifentanil or sevoflurane anesthesia on patients undergoing radical gastrectomy.

    abstract::Anesthetic effect of propofol combined with remifentanil or sevoflurane intravenous anesthesia on patients undergoing radical gastrectomy was evaluated. The clinical data of 516 cancer patients who received radical gastrectomy in the First Bethune Hospital of Jilin University between January 2011 and December 2017 wer...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.10238

    authors: Feng Y,Li J,Wang H,Duan Z

    更新日期:2019-06-01 00:00:00

  • Primary non-Hodgkin lymphoma of the right femur and subsequent metastasis to the left femur: A case report and literature review.

    abstract::Non-Hodgkin lymphoma of the bone is rare and typically causes an extensive bone lesion. The present study describes a case of diffuse large B-cell primary non-Hodgkin lymphoma of the bone, which occurred in the right femur, and was initially treated with surgery and chemotherapy. Following a 7-year period of complete ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2018.7895

    authors: Hu JY,Yu D,Wu YH

    更新日期:2018-04-01 00:00:00

  • Expression of DNA topoisomerase II-α: Clinical significance in laryngeal carcinoma.

    abstract::DNA topoisomerase II-α (Topo II-α) is essential for numerous cell processes, including DNA replication, transcription, recombination, and chromosome separation and condensation. Altered Topo II-α expression may lead to carcinogenesis and cancer progression. The aim of the present study was to investigate the associati...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2014.2367

    authors: Feng Y,Zhang H,Gao W,Wen S,Huangfu H,Sun R,Bai W,Wang B

    更新日期:2014-10-01 00:00:00

  • Significance of gastrin-releasing peptide in ovarian cancer ES2 cells.

    abstract::The present study aimed to investigate the effect of gastrin-releasing peptide (GRP) on the proliferation and invasion of ovarian cancer ES2 cells. The ovarian cancer ES2 cells were transfected with small interfering RNA against GRP. Cell proliferation was assessed using the Trypan blue assay, apoptosis was determined...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2015.3240

    authors: Jia Y,Shi H,Fan D

    更新日期:2015-07-01 00:00:00

  • Interferon-α suppresses invasion and enhances cisplatin-mediated apoptosis and autophagy in human osteosarcoma cells.

    abstract::Interferon (IFN)-α is generated in response to viral infections and is used clinically in the therapy of a variety of viral infections and cancers. The present study investigated whether IFN-α could inhibit the invasive ability of osteosarcoma cells using a Matrigel invasion assay. In addition, the osteosarcoma cells ...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1762

    authors: Zhao J,Wang M,Li Z,Chen J,Yin Z,Chang J,Gao D,Wang S

    更新日期:2014-03-01 00:00:00

  • Bilateral breast cancer with a unilateral carcinoma within a fibroadenoma: A case report.

    abstract::Fibroadenomas are a type of benign tumor that occur in young women below the age of 35 years old. The tumors are the second most common type of tumor after fibrocystic disease. The chance of carcinoma arising in a fibroadenoma is extremely low. To date, <130 such cases have been reported. Previous studies have reporte...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2015.3420

    authors: Zheng H,Chen J,Wu X,Jin L,Qi C

    更新日期:2015-09-01 00:00:00

  • Distinct prognostic values and antitumor effects of tumor growth factor β1 and its receptors in gastric cancer.

    abstract::Gastric cancer (GC) is one of the most common malignancies and is the second leading cause of cancer-associated mortality world-wide. In the present study, the prognostic value and antitumor effects of transforming growth factor β1 (TGFβ1) and its receptors in GC were explored. The online Kaplan-Meier plotter database...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2020.11849

    authors: Liu F,Wang H,Zhang M

    更新日期:2020-09-01 00:00:00

  • Expression of p53, epidermal growth factor receptor, Ki-67 and O6-methylguanine-DNA methyltransferase in human gliomas.

    abstract::The present study aimed to evaluate the expression of p53, Ki-67, epidermal growth factor receptor (EGFR) and O6-methylguanine-DNA methyltransferase (MGMT), and to analyze the correlation between their expression and the histological grade of the tumors in 152 patients with gliomas. The tumors were classified accordin...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2013.1317

    authors: Hu X,Miao W,Zou Y,Zhang W,Zhang Y,Liu H

    更新日期:2013-07-01 00:00:00

  • Fluoroscopy-guided long intestinal tube placement for the treatment of malignant bowel obstruction.

    abstract::The aim of the present study was to investigate the safety and efficacy of long intestinal tube placement under fluoroscopic guidance for the treatment of malignant bowel obstruction (MBO). The cases of 74 patients with MBO who underwent long intestinal tube placement under fluoroscopic guidance during the period betw...

    journal_title:Oncology letters

    pub_type: 杂志文章

    doi:10.3892/ol.2019.10151

    authors: Lai H,Wu K,Liu Y,Zeng Z,Zhang B

    更新日期:2019-06-01 00:00:00