Abstract:
:Glycocholic acid (GCA) is a novel identified biomarker for hepatocellular carcinoma (HCC). However, clinical pathological study of GCA has not been extensive due to the limited availability of anti-GCA monoclonal antibodies (mAbs) and restricted detection methods. In the present study, using human GCA conjugated with bovine serum albumin as the immunogen to immunize BALB/c mice, a novel anti-GCA mAb was generated and characterized. The isotypes of heavy chain and light chain of anti-GCA mAb were examined to be IgG2a and κ, respectively, with a high affinity constant (2.6×108 mol/l). The anti-GCA mAb binds GCA with high specificity and sensitivity, and the 50% inhibitory rate was 77.09 ng/ml. The present study also established a rapid, sensitive and efficient indirect competitive ELISA analysis using this anti-GCA mAb to detect the level of GCA produced by different HCC cell lines. Therefore, the present study may successfully develop a novel method for early HCC diagnosis, and also provide insights for further research and treatment of HCC.
journal_name
Oncol Lettjournal_title
Oncology lettersauthors
Wu M,Zhai S,Gao J,Wei D,Xue J,Zhou Y,Li N,Hu Ldoi
10.3892/ol.2019.9943subject
Has Abstractpub_date
2019-03-01 00:00:00pages
3103-3112issue
3eissn
1792-1074issn
1792-1082pii
OL-0-0-9943journal_volume
17pub_type
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